discussion this week: biochemical pathways splicing rna ...€¦ · rna poi ll. rna pol il...
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Discussion this week:
biochemical pathways splicing RNA processing
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transcript modifications in eukaryotes
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co-transcriptional (aka post-transcriptional)
pre-mRNA processing
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TFIIH
(YSPTSPS)52
C-terminal domain
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initiation
splicing
capping
polyadenylation
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• protects against degradation • ribosome binding
• generates diversity
• transcription termination • transport to cytoplasm • mRNA stability • translation efficiency
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“transcription unit”
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transcription unit complexity: splicing
splicing: producing the “translatable” protein coding region(s)
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intron intron intron exon exon exon exon
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“Problem 5.15”
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Consensus sequences necessary for correct splicing
Nomenclature: junctions named relative to INTRONS
splice junction
5´ donor 3´ acceptor
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spliceosome assembly
snRNAs + proteins = snRNPs (snurps)
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EM image of 2 genes being transcribed
rRNA genes
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