digital analysis of quadrats to determine percent cover of metaphyton in conesus lake, ny
DESCRIPTION
Digital Analysis of Quadrats to Determine Percent Cover of Metaphyton in Conesus Lake, NY. Alternate method for accurate determination of percent cover. Michael Pagano Dr. Sid Bosch State University of New York at Geneseo Summer 2003. Problem at Conesus Lake- Metaphyton. - PowerPoint PPT PresentationTRANSCRIPT
Digital Analysis of Quadrats to Determine Percent Cover of
Metaphyton in Conesus Lake, NY Alternate method for accurate determination of percent cover
Michael Pagano
Dr. Sid Bosch
State University of New York at Geneseo
Summer 2003
Problem at Conesus Lake- Metaphyton
• Correlation between growth of metaphyton with nutrient loading from streams
Problem with determination of percent cover
• Metaphyton biomass hard to estimate
• Entangled in Milfoil, can’t separate
• Percent Cover best estimation
Traditional Method
• Visual determination of cover
• Stationed off side of boat
• Dependant on researcher
• Lack of precision
Alternate Method• Construction of new quadrat (.5x.5m) to enable
use of digital camera to capture image of algae• Camera mounted Tri-pod • Polarized lens used to reduce glare
Alternate Method
• Digital Pictures (3.2mp) uploaded onto computer
• Images enhanced using Kodak Photo Enhancer
Alternate Method• Images then analyzed using Image
J to determine percent cover
Percent Cover=
Total Cover Total Area
Results2003 Metaphyton
0
20
40
60
80
100
120
perc
ent c
over
Error bars indicate one standard deviation above and below mean
Bars above represent results of Tukey’s Statistical Analysis
ANOVA p<0.05
Results
0
10
20
30
40
50
60
70
80M
ea
n P
erc
en
t M
eta
ph
ton
co
ve
r
2001
2002
2003
Graywood 2003, Cottonwood 2002, Sandpoint 2002, & Sutton 2002 not used due to sampling error which included date and condition of weed beds. Numbers above bars represent one standard deviation
7
40
24
14
25
21
27
35
306.5
29
15
32
37
Results
No loading data for McPherson, Graywood Gully not used for statistical purposes because unrepresentative sampling period; Error Bars (+/- 1 S.D.) to small to see
R2 = 0.8806
0
10
20
30
40
50
60
70
0.00 5.00 10.00 15.00 20.00
Loading SRP (6/1-8/31, 2003) Kg
Pe
rce
nt
Co
ve
r M
eta
ph
yto
nP=0.06
Metaphyton Cover Determination• Is Digital Analysis more accurate than traditional
visual estimation?
Results• Coefficient of Variation
– CV= S.D./Mean– Measure of Relative Variability
Organism Coefficient of Variation
Plankton 0.70
Benthic Organisms (grab sample) 0.40
Benthic Organisms
(Surber sampler, counts)
0.60
Benthic Organisms
(Surber sampler, biomass)
0.80
Terrestrial Organisms
(Roadside Counts)
0.80
Shellfish 0.40
Alternate Method
0
0.2
0.4
0.6
0.8
1
1.2
1.4
Coe
ffici
ent o
f Var
iatio
n
2001
2002
2003
Mean C.V.
2001= 0.883319 +0.385
2002= 0.979605 + 0.110
2003= 0.397958 + 0.137
Conclusions
• No consistent trends seen between weed beds
• Correlation seen between metaphyton percent cover and summer SRP loading, 2003
• Alternate method more accurate for determination of percent cover, but more replicates needed
Special Thanks
• Dr. Sid Bosch, Mentor and Project Advisor
• Megan Mongiovi, Jamie Romieser, Evan Zynda, Student Researchers
• SUNY Geneseo Biology