development of local and scanning probe techniques heinrich hoerber nanobiophysics university...
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Development of Development of Local and Scanning Local and Scanning Probe TechniquesProbe Techniques
Heinrich HoerberNanoBioPhysicsUniversity Bristol
200 nm
Haberle, W., J. K. H. Horber, and G. Binnig, 1991, Journal of Vacuum Science & Technology B 9, 1210
Combining AFM and Combining AFM and optical microscopyoptical microscopyCombining AFM and Combining AFM and optical microscopyoptical microscopy
AFM image sequence of a pox virus release at the end of a microvillus
EM image by Stokes 1976
Scanning fast - making Scanning fast - making moviesmoviesScanning fast - making Scanning fast - making moviesmovies
Horber, J.K.H., et al., 1992, Scanning Microscopy 6, 919
Horber, J. K. H., W. Haberle and
B. Sakmann, 1995 Biophysical Journal 68, 1687
Combination with Combination with electro-physiological techniqueselectro-physiological techniquesCombination with Combination with electro-physiological techniqueselectro-physiological techniques
Koitschev, A., S. Fink, U. Rexhausen, K. Loffler, J. K. H. Horber, H. P. Zenner, J. R. Ruppersberg, and M. G. Langer, 2002, Hno 50, 464-469
AFM in hearing AFM in hearing researchresearchAFM in hearing AFM in hearing researchresearch
Cantilever Cantilever developmentdevelopmentCantilever Cantilever developmentdevelopment
IBM Research Laboratory RueschlikonM. Despont, G. Binnig, P. Vettiger and C. Gerber
Heat flow between Heat flow between cantilever and substratecantilever and substrate
Heat flow between Heat flow between cantilever and substratecantilever and substrate
Heat transfer throughcantilever arms (~4 mW)
Cantilever with heater
through tip in contact 50-500 nW
Through air to substrate (10-20 μW)
50-5000C
ΔR/R: 10-6–10-5/nm
2 μm
Lipid vesicles with reconstituted membrane proteins (SNAP25, B.Jena)
2 μm1 μm
SiC GaN
Metal connection on a storage chip structure underneath a SiO2 layer(Zarlink)
Cut through a transistor structure(M. Kubal)
Heat conductivityHeat conductivityHeat conductivityHeat conductivity
Haeberle, W, Pantea, M & Hoerber, JKH. 2006, Ultramicroscopy, 106 (8-9), 678
100 m 1 m
length 260 m, width 1 m, thickness 200 nm, spring constant 0.03 pN/nm
Smaller cantileversSmaller cantileversSmaller cantileversSmaller cantilevers
James Vicary
AFM with the cantilever AFM with the cantilever verticalverticalAFM with the cantilever AFM with the cantilever verticalvertical
Massimo Antognozzi, Arturas Ulcinas
Maximal cantilever bending
Molecular Motor Molecular Motor movementmovementMolecular Motor Molecular Motor movementmovement
Massimo Antognozzi, Tim Scholz
0.5 nm
200 nm
200 μm
0.02 N/m
Florin, E. L., A. Pralle, E. H. K. Stelzer, and J. K. H. Horber, 1998, Applied Physics A-Materials Science & Processing 66, S75
Spatial resolution Spatial resolution ~ 1nm~ 1nmTime resolution ~ 1Time resolution ~ 1μμsecsec
PPhotonic hotonic FForce orce MMicroscopyicroscopyPPhotonic hotonic FForce orce MMicroscopyicroscopy
Agarose polymer networkAgarose polymer network
Tischer, C. et al., 2001, Appl. Phys. Lett. 79, 3878
Thermal fluctuation Thermal fluctuation imagingimagingThermal fluctuation Thermal fluctuation imagingimaging
Membrane diffusion 2D / Membrane diffusion 2D / 3D3DMembrane diffusion 2D / Membrane diffusion 2D / 3D3D
Confining potential map
Viscosity map
Interaction map of an LDL Interaction map of an LDL receptorreceptorInteraction map of an LDL Interaction map of an LDL receptorreceptor
Parallelisation of Parallelisation of PFMPFMParallelisation of Parallelisation of PFMPFM
Producing many Producing many independent independent focused laser beamsfocused laser beams
Producing many Producing many independent independent focused laser beamsfocused laser beams
Nano-particles crossing Nano-particles crossing the cell membranethe cell membrane
Nano-particles crossing Nano-particles crossing the cell membranethe cell membrane
Types of nano-Types of nano-particlesparticlesTypes of nano-Types of nano-particlesparticles
Gold nano-particles Gold nano-particles
interacting with interacting with lightlight
Gold nano-particles Gold nano-particles
interacting with interacting with lightlight
Nearfield fluorescence Nearfield fluorescence excitationexcitationNearfield fluorescence Nearfield fluorescence excitationexcitation
Raman Spectroscopy
Chemical sensingChemical sensingChemical sensingChemical sensing
Surface EnhancedRaman Spectroscopy
Nano-Sensors (Scanning Probe Microscopies)
Nano-toxicology
Nano-medicine (markers, drug delivery)
Nano-particles - areas of interestNano-particles - areas of interest