development and use of novel semiochemical technologies ...€¦ · tuzak (trimedlure) novel...

Development and Use of Novel Semiochemical Technologies for Integrated Pest Management:

Global and Local Perspectives

Orkun Baris Kovanci, Ph.D.

Uludag University, Faculty of Agriculture, [email protected]

1 BIOTECHNICAL METHODS

USE OF SEMIOCHEMICALS WORLDWIDE2

3

4

CONTENTS

NEW TECHNOLOGIES, TECHNIQUES & PRODUCTS

THE FUTURE OF BIOTECHNICAL CONTROL

BIOTECHNICAL CONTROL

• Biotechnical control is based on the methods that disrupt the biology, physiology and behavior of the target pest species.

MALE

FEMALE

PHERO-DISPENSERS

Kairomone?

Pheromone?

• It involves the use of semiochemicals such as pheromones, repellents and oviposition deterrents.

do Nascimento, R. R.; Morgan, E. D. Quim. Nova 1996, 19, 156-65.

SEMIOCHEMICALS

Semiochemicals

Pheromones Allelochemicals

Semiochemicals (Gk. semeon, meaning mark or signal)

Pheromones (Gk. pherin, to carry and hormon, to excite)

Allelochemicals (Gk. allelon, of one another)

Kermania pistaciella

(2S,12Z)-2- Acetoxy-12-heptadecene

DIRECT

DIRECT

DIRECT

INDIRECT

DIRECT & INDIRECT USE OF BIOTECHNICAL METHODS

4. ATTRACT & KILL

3. MATING DISRUPTION

2. MASS-TRAPPING

1. MONITORING

MONITORING

Medfly traps containing Trimedlure

Akdeniz meyve sineği

sarı delta tipi tuzak

(Trimedlure)

McPhail traps baited with amonium acetate, putrescine and trimethyl amine

Bactrocera oleae

MONITORING



(Trimedlure) Kairomones: (E)-anetol, (E)-cinnamon Ephestia spp. Dendroctonus spp.Tropinota hirta

Oriental fruit moth

Hoplocampa spp.

Ostrinia spp.

MONITORING



(Trimedlure)

Tuta absoluta trap developed in Demreli, Antalya TUTA-R

Flight activity of the Oriental fruit moth in İnegöl, Bursa during 2006-2009

0

50

100

150

200

250

0

10

20

30

40

50Egg/Sam

pleAdult Egg

MAR APR MAY JUN JUL AUG SEP OCT

Mot

h/Tr

ap

MONITORING FOR QUARANTINE

♀♂ ♂

MCA (4- methoxy-cinnamaldehyde)

kairomone or female sex pheromone (8-methyl-decan-2-ol propionate)

Diabrotica spp.

COMBINED TRAPPING



(Trimedlure)

BAITS FOR MONITORING?



(Trimedlure) Ephestia spp.

Pear ester: Etil (E,Z)-2,4-dekadienoat >

Terpinyl acetate > Oriental fruit moth Apple vinegar > Drosophila suzukii

MASS TRAPPING



(Trimedlure)

0 3 3-30 > 30 Tuta MOTHS CAUGHT

MASS TRAPPING (40 traps/ha)

MASS TRAPPING (20-40 traps/ha)

MASS TRAPPING (20 traps/ha)

NO INFESTATION

HOW MANY TRAPS AT VARYINGTUTA DENSITY?

FRUIT DAMAGE (%) (Aksoy and Kovanci, 2016)

a

a

C

1

2

3

0

4

5

6

FRU

IT D

AM

AG

E (%

)

Mass trapping(40 traps/ha)

Insecticide

Mass trapping low-density populations of Tuta absoluta with various types of traps in field-grown tomatoes

Journal of Plant Diseases and Protection 123 (2), 51-57.

https://scholar.google.com/citations?view_op=view_citation&hl=en&user=wfQmRgIAAAAJ&cstart=20&pagesize=80&citation_for_view=wfQmRgIAAAAJ:7PzlFSSx8tAC

MATING DISRUPTION: SPRAYABLE PHEROMONES



(Trimedlure)

Pheromone Capsule wall Water

ULTRA-LOW VOLUME

HIGH-VOLUME APPLICATION

0

5

10

15

20

LOW HIGH LOW HIGHLEAF POSITION

HIGH-VOL

ULV

NO

MIC

RO

CA

PS

ULE

S

PE

R L

EA

FCanopy (Lower < 2m) Canopy (Higher than > 2m)

ULV and high-volume sprayable pheromone applications in İnegöl, Bursa during 2006-2008

MATING DISRUPTION: SPRAYABLE KAIROMONES (Kovanci, 2015)



(Trimedlure)

Co-application of microencapsulated pear ester and codlemone for mating disruption of Cydia pomonella

Journal of Pest Science 88 (2), 311-319

https://scholar.google.com/citations?view_op=view_citation&hl=en&user=wfQmRgIAAAAJ&citation_for_view=wfQmRgIAAAAJ:LkGwnXOMwfcC

MATING DISRUPTION: AEROSOL PUFFERS



(Trimedlure)

MATING DISRUPTION: HAND-APPLIED DISPENSERS



(Trimedlure) Stethorus spp.

MATING DISRUPTION: MOTH CATCHES AT DIFFERENT TREATMENTS



(Trimedlure)

0

510

15

20

2530

35

6-May

20-May

3-Jun

17-Jun

1-Jul

15-Jul

29-Jul

12-Aug

26-Aug

9-Sep

23-Sep

Mea

n cu

mul

ativ

e ca

tch/

trap

a HV sprayableLV sprayableHand-appliedInsecticide

a

b c

d

Mean cumulative codling moth pheromone trap captures in, conventional insecticide treated, and insecticide + mating disruption blocks

averaged across two orchards in Bursa in 2012.

Damageb (%)Treatment Sting Entry Live worm

Checkmate CM-F HV spray 0.9 a 0.9 a 0.1 aCheckmate CM-F LV spray 0.9 a 0.3 a 0.0 aCheckmate CMXL1000 0.0 a 0.0 a 0.0 aInsecticide 1.3 a 1.0 a 0.5 a

FRUIT DAMAGE ASSESMENT

Mean percentage fruit damage by codling moth larvae in conventional insecticide treated, and reduced insecticide + mating disruption blocks averaged across two locations in Bursa, northwestern Turkey in 2012.

b Means with the same column followed by the same letter are not significantly different by Fisher’s protected LSD test (P < 0.05). Data were analyzed using arcsine square root, but data shown are back transformations.

MATING DISRUPTION: WATER SOLUBLE PASTE FORMULATIONS



(Trimedlure)

MATING DISRUPTION WORLDWIDE



(Trimedlure)

MATING DISRUPTION IN VINEYARDS



(Trimedlure)

ATTRACT & KILL



(Trimedlure)

PHEROMONE + KAIROMONE AGAINST RED PALM WEEVIL

ATTRACT & KILL



(Trimedlure)



(Trimedlure)

NOVEL TECHNOLOGIES: ORGANIC NANOFIBERS

NOVEL TECHNOLOGIES: ORGANIC NANOFIBERS



(Trimedlure)

NOVEL TECHNOLOGIES: PHEROMONE NANOGELS



(Trimedlure)



(Trimedlure)

PARAPHEROMONES




(Trimedlure)




(Trimedlure)

NOVEL TECHNOLOGIES: RECYCLABLE DISPENSERS

• Anfora et al. (2008) used Ecodian LB dispensers.• Emitters were loaded with 10 mg (E, Z) -7,9-dodecadienyl

acetate and made from low-priced recyclable material to control Lobesia botrana.

• The efficacy of mating disruption lasted for 60 days.



(Trimedlure)

NOVEL TECHNOLOGIES: BRANCH FITTED DISPENSERS



(Trimedlure)

• Suckling et al. (2011) noted that pheromone-loaded sterile Ceratitis capitata (Wied.) adults may interfere with sexualcommunication of Epiphyas postvittana males.

• (E) -11-tetradecenyl acetate were released by sterile medflies to prevent adult males of E. postvittana from being caught in synthetic pheromone delta traps.

• Mating disruption in the first 4 nights were 95, 91, 82 and 85%, respectively.

NOVEL TECHNOLOGIES: MOBILE MATING DISRUPTION

NOVEL TECHNOLOGIES: ELECTROANTENNOGRAM MEASUREMENTS



(Trimedlure)

NOVEL TECHNOLOGIES: DRONES TO APPLY PHEROMONES?



(Trimedlure)

PUSH & PULL TECHNIQUE



(Trimedlure)

(Sorghum sudanese)

(Busseola spp.)

CAN SEMIOCHEMICALS REPLACE TRAP CROPS IN THE FUTURE?

NOVEL TECHNOLOGIES: ATTRACTING NATURAL ENEMIES



(Trimedlure)

GOOD AGRICULTURAL PRACTICES OFFERBRIGHT FUTURE FOR FARMING!

THANK YOU FOR YOUR

ATTENTION!!

Diapositive numéro 1Diapositive numéro 2BIOTECHNICAL CONTROL SEMIOCHEMICALSDiapositive numéro 5Diapositive numéro 6Diapositive numéro 7Diapositive numéro 8Diapositive numéro 9Diapositive numéro 10Diapositive numéro 11Diapositive numéro 12Diapositive numéro 13Diapositive numéro 14Diapositive numéro 15Diapositive numéro 16Diapositive numéro 17Diapositive numéro 18ULV and high-volume sprayable pheromone applications �in İnegöl, Bursa during 2006-2008Diapositive numéro 20Diapositive numéro 21Diapositive numéro 22Diapositive numéro 23FRUIT DAMAGE ASSESMENTDiapositive numéro 25MATING DISRUPTION WORLDWIDEMATING DISRUPTION IN VINEYARDSDiapositive numéro 28Diapositive numéro 29Diapositive numéro 30Diapositive numéro 31Diapositive numéro 32Diapositive numéro 33Diapositive numéro 34Diapositive numéro 35Diapositive numéro 36Diapositive numéro 37Diapositive numéro 38Diapositive numéro 39Diapositive numéro 40Diapositive numéro 41Diapositive numéro 42Diapositive numéro 43

development and use of novel semiochemical technologies ...€¦ · tuzak (trimedlure) novel...

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