designing affinity ligands for downstream process intensification
TRANSCRIPT
![Page 1: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/1.jpg)
Seite 1
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Designing Affinity Ligands for Downstream Process Intensification
Prof. Dr. Hans Henning von Horsten
HTW-Berlin University of Applied Scienceswww.htw-berlin.de
Prof. Dr. Hans Henning von Horsten
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
![Page 2: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/2.jpg)
Seite 2
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Key drivers of process intensification
FlexibilityProductivityCost effectiveness Consistent Product Quality
Conventional Batch Unit Operations
Continuous Unit Operations
n cumulative cycles
ProcessIntensification
Consistent Product Quality
![Page 3: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/3.jpg)
Seite 3
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
ICHQ8 (R2)
Critical quality attribute (CQA): A physical, chemical, biological, ormicrobiological property or characteristic that should be within anappropriate limit, range, or distribution to ensure the desired productquality( ).
CDR-integrity
N-Glycan Profile
K-Clipping
Deamidation
OxidationDisulfideShuffling
Glycation
Aggregation
Adventitious viruscontamination
AdventitiousTSE
Endotoxin
Residual DNA
Residual HCP
Column Leachables/Protein A
IsoaspartateRacemization
Critical Quality Attributes
Bioburden
![Page 4: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/4.jpg)
Seite 4
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
ContinuousBioreactor /
Cell Retention
NF
Pool
PCC-cycle 1
Low pH-EluateHold
Protein A AffinityCapture
PCC-cycle 2
PCC-cycle 3
IEX-1 IEX-2
UF/DF
Intensified Process: Semi-Continuous Process Operation
BulkDS
![Page 5: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/5.jpg)
Seite 5
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Load Elute
ColumnSwitch
Load
Periodic Countercurrent Chromatography
ReequilibrationCycle
I II II I
���� Intensified use of
capture capacity
![Page 6: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/6.jpg)
Seite 6
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Periodic Countercurrent Chromatography
I II II I
Surface induced conformational changes
High on-resin protein concentrations matching resin capacity
Denaturing elution at low pH
Aggregates/HMM-species
PCC����Cumulative entrapment of aggregation seeds after multiple runs
![Page 7: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/7.jpg)
Seite 7
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Protein A-induced product aggregation
Fig. 2a: SEC Profiles illustrating increased vulnerability of protein A-purifed IgG by exposure to pH 3.0
Gagnon et al., J. Chromatogr., A 1395 (May 2015): 136–142.
![Page 8: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/8.jpg)
Seite 8
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Protein A-induced product aggregation
Fig. 2b: Semi-log plot of monomer decay rate, R0, against pH for solution-only
experiments (filled triangles) and experiments including a prior protein A
chromatography step (open circles).
Mazzer et al., J. of Chrom A, Vol 1415, 9 Oct 2015, pp 83–90
![Page 9: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/9.jpg)
Seite 9
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Alternative Affinity Ligand Design
![Page 10: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/10.jpg)
Seite 10
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Prerequisites:
Reasonable selectivity
Mild nondenaturing elution
High capacity binding
Non-immunogenic leachables
Alternative Affinity Ligand Design
Possible Solution:
� multivalent ligand binding
� competitive elution
� LMM-Ligand: enhanced ligand density
� Carbohydrate-Ligands
![Page 11: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/11.jpg)
Seite 11
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Alternative Affinity Ligands
I. Arylboronic acids
*
**
Dennis Hall, 2005* Boron Science New Technologies and Applications Narayan S. Hosmane (Ed.) Crc Pr Inc; 3. Oktober 2011** Smoum et al., Magn. Reson. Chem. 2003; 41: 1015–1020
X
II. Coordinated transition metal ions
![Page 12: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/12.jpg)
Seite 12
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Alternative Affinity Ligand Concept
Dennis Hall, 2005
![Page 13: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/13.jpg)
Seite 13
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Alternative Affinity Ligand Concept
Dennis Hall, 2005
Binding Mediators
I. Aryldiboronic Acids
II. Transition Metal Ions
X
![Page 14: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/14.jpg)
Seite 14
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Alternative Affinity Ligand Concept
Arylboronic acid functionalized ligands: Bivalent Ligand Concept
![Page 15: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/15.jpg)
Seite 15
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
+
Binding
Alternative Affinity Ligand Concept
![Page 16: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/16.jpg)
Seite 16
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
+
Competitive Elution:(Cis-diols/
alpha-hydroxycarboxylates)
Alternative Affinity Ligand Concept
R
R
![Page 17: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/17.jpg)
Seite 17
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Lactate - no longer a problem
Yuk et al., 2014
![Page 18: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/18.jpg)
Seite 18
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
Investigation of Affinity Ligand Performance
![Page 19: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/19.jpg)
Seite 19
Prof. Dr. H. Henning von HorstenHTW-Berlin University of Applied Sciences
3rd Biologics & Biosimilars Congress, March 6th-7th, 2017, Berlin, Germany
[Source: PALL/Fortebio]
Biolayer Interferometry
time
nm
shift
Baseline Association Dissociation
![Page 20: Designing affinity ligands for downstream process intensification](https://reader034.vdocuments.site/reader034/viewer/2022042723/58e4f3bf1a28abdf698b4783/html5/thumbnails/20.jpg)
Seite 20Seite 20
www.htw-berlin.de
3rd Biologics & Biosimars Congress ThankYou!
Prof. Dr. Hans Henning von
Horsten
+49-30-5019-3636
Life Science Engineering
HTW-Berlin University of Applied Sciences
Wilhelminenhofstraße 75A,
12459 Berlin