dental caries (i): the...
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Dental Caries (I):The Etiology
Early Theories of Caries Etiology1.Worms: before B. C.2.Humors: four elemental fluids of the body3.Vital theory: analogous to bone gangrene 4.Chemical theory: inorganic acids corroded
enamel 5. Parasitic or septic theory: filamentous parasite6.Chemo-parasitic theory: blend of two theories7.Proteolytic theory: organic or protein elements
are the initial pathway of invasion by microorgasms
8.Proteolysis-chelation theory9.Other theories: bacteria take up phosphate
Current Concepts of Caries Etiology
Dental caries is a multi-factorial disease1) The Host : the saliva and teeth2) The microflora : S. mutans3) The substrate or diet : fermentable
carbohydrate4) Time: the duration of teeth attacked by
organic acid
Development of Dental Caries
food + dental plaque acid (carbohydrate) (bacteria)
production demineralization of
teeth cavities dental caries
Host factors: The Saliva and Dental Caries• Effect of desalivation on incidence and
extent of caries in animals• Decreased salivary flow and caries in
humans (Xerostomia)
Critical pH=5.5
The pH at which any particular saliva ceases to be saturated with calcium and phosphate is referred to as the critical pH; below this value the inorganic material of the tooth may dissolve.
PROTEIN AND NONPROTEIN CONSTITUENTS OF SALIVA
AlbuminAmmoniaAmylaesBicarbonateBeta-glucuronidaseCalcium ChlorideCreatinine CystatinsEsterasesFluorideGlucoseGustin
Nonspecific buffersPhosphatasesPhosphorusPotassiumProline–rich proteinsRibonucleasesSerum proteins (trace)Sialic acidSodiumStatherinSulfatesThiocyanateUric acid
HistatinsIgA (sIgA)IgGIgMIodineKallikreinLactoferrinLactoperoxidaseLDHLysozymeMagnesiumMucinsNitrogen
FUNCTIONS OF SALIVA
Function LubricationAntimicrobial
Remineralization
CleansingBufferingDigestive
Mucosal Integrity
Salivary Components Involved Mucins, Proline-rich proteins,H2OLactoferrin, Lysozyme, Lactoperoxidase, sIgA, Mucins, Histatins, Cystatins, Proline-rich proteinsCa, P, Pi, Statherin, Anionic proline-rich proteinsH2OHCO3, PO4Amylase, Lipase, Proteases, H2O, Nucleases, Mucins, Gustin
H2O, Electrolytes, Mucins
Salivary Composition and Caries
1. Salivary buffers– Bicarbonate (HCO3
- / H2CO3)– Phosphate (HPO4= / H2PO4
-)– Urea → Ammonia
2. Antibacterial factors– Lysozyme– Lactoferrin– Salivary peroxidase system– Immunoglobulins
Immunization :
The animal studies of immunization with killed cariogenic bacteria, can be divided into two groups:
1) Those involving stimulation of secretory IgA, either directly, locally, or indirectly via the gut.
2) Those involving stimulation of serum antibodies, which reach the plaque via the gingival sulcus.
Route of Immunization
1. Local immunity (direct stimulation of slgA)2. Oral immunization (indirect stimulation of
slgA)3. Stimulation of serum antibodies (IgG)4. Passive immunity
Host Factors : Tooth
Morphology : pit and fissure areas
Host factors : Tooth
Susceptibility to caries between different tooth types:
mandibular first molars (occlusal > buccal > mesial > distal > lingual) > mand. 1st molars > max. 1st molars >mand. & max. 2nd molars > 2nd premolars > max. incisors > first premolars > mand. incisors and canines
Arch form:
Irregularities in arch form, crowding and overlapping of teeth favor the development of caries lesions.
Tooth composition :
Enamel surface is more caries-resistant than the subsurface (more minerals).
Partially fluoridated hydroxyapatite: more resistant to demineralization
Bacterial Role in Caries EtiologyS. mutans has been suggested to play an
important role in the initiation of dental caries in human. Its cariogenicity is due to the bacterium’s capacity to produce various acid from dietary sugars with a resultant demineralization of tooth enamel.
Root Caries
Roots caries :1.Starts at or near the cementoenamel junction2. Appears only after cementum is exposed3. The frequency of involvement of a specific
surface being dependent upon the tooth type 4. Attack mand. molars most frequently, followed
by mand. premolars and max. canines, the mand. incisors being the least frequently involved teeth
5. Usually does not involve enamel
Bacterial interactions︰Veillonella alcalescens
Lactate → propionic and acetic acids
Bacteriocins are active against some strains of the same or closely related species, but not against unrelated species.
Bacterial interactions
Substrate: Diet and Dental Caries
Foods and beverages serve as substrates for fermentation by the plaque microflora, which form organic acids, thereby promoting demineralization of tooth structure and directly affecting caries activity. Patients with rampant caries frequently include sucrose–containing foods in their diet.
Influence of diet on the caries process:Epidemiology observation1.Sucrose consumption and prevalence of
caries2. Strict dietary control –> less caries3. Hereditary fructose intolerance (HFI) –>
less caries4. Bakeries and candy factories –> high
caries
Sucrose ←→ Caries
• Determining sucrose content• Cariogenicity of sucrose and other
carbohydrates
Adhesiveness of foods:
Sticky or adhesive forms sucrose –containing foods, which can maintain high sugar levels in the mouth, were more cariogenic than those forms that were rapidly cleared.
Ranking the foods:Cariogenic potential of foodstuffs
Using currently accepted methods (primarily plaque pH measurements and animal testing), foods can be categorized as having: 1. No cariogenic potential2. Low cariogenic potential3. High cariogenic potential
Conclusion :
The physical form, consistency, and frequency of intake, as well as the sugar content, are major determinants of the cariogenicity of foods.
Other Dietary Components and Dental Caries:A) Phosphates:possible caries–preventive
agents1. Reduce the rate of dissolution of the
hydroxyapatite2. Redeposit calcium phosphate, particularly in
areas of enamel that have been partially demineralized
3. Buffer organic acids4. Desorb proteins from the enamel surface,
thereby modifying the acquired pellicle
B) Lipids:Medium chain fatty acids (C8 – C12) and their salts have antibacterial properties at low pH
C) Trace elements:
Dental Caries (II):Prevention
Caries prevention is based upon attempts to 1) Increase the resistance of the host:
fluoride therapy, occlusal sealants, immunization.
2) Lower the numbers of microorganisms in contact with the tooth:plaque control
3) Modify the substrate by selecting non-cariogenic foodstuffs
4) Reduce the frequency of intake sucrose
Approaches for controlling dental cariesFissure sealants– Occlusal pits and fissures︰ the most
caries-prone areas– For children
Fluoride– Water supply︰reduce the incidence of
caries by at least 50%
– Optimum concentration︰1 ppm
– Table salt, milk, toothpastes
– Mouthwash, gel, and vanish for topical
use
– Tablets︰ systemic effect
– Tea, fish bone
Fluoride– 90% absorbed by gut into blood– 50% rapidly excreted by kidneys – deposit in skeletal tissue, un-erupted
enamel, oral fluid– Fluoro-apatite resists acid– Inhibit bacteria acid production (enolase)
Dental Caries Vaccine
– Oral cavity has all the components for an immune response
– Formalin– or heat– killed S. mutans injected into the region of the salivary glands in primates
– Salive︰ sIgA, crevicular fluid︰IgG– Drawback︰ form Ab-Ag complex associates
with cardiac muscle– Damage to heart and kidney– Four protein in the cell well
Mechanisms︰unclear
– inhibiting the colonization– enhance phagocytosis by PMN– interfere bacterial metabolism
Question to anti – caries vaccine
– Non-life-threatening disease– Efficacy of vaccine in humans – Specificity: many species of bacteria can
cause caries– Cross reaction with heart tissue– Other approaches can control dental
caries
Antibiotics and antmicrobial agents
– Long-term antibiotic therapy → low caries experience
– May develop resistant-organisms– Overgrowth by opportunistic pathogens
Chlorhexidine
– inhibitory to fungi and wide range of Gram(+) and Gram(-) oral bacteria
– High concentration︰bactericidal, damaging the cell membrane
– low concentration︰bacteriostatic, reduce acid production by dental plaque
– S. mutans︰sensitive, S. snaguis︰resistance
Artificial sweetenersTwo typesI. Intense type︰– sweeter than sucrose– saccharin, cyclanates– carcinogenesis in rodents
II. Bulk agents
– not as sweet as sucrose– mannitol and sorbitol︰metabolized by S.
mutans slowly, six carbon cyclical structure
– xylitol︰not metabolize by plaque bacteria, five carbon cyclical structure
Reduce the frequency of Intake sucrose
Caries activity tests
Thank you for your attention