denmark

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Soren ( focus on financials, investments,potential collaboration) 1. Why did they go bankrupt? their mistakes which we should not repeat? 2. How much valuation can we expect with our team ,our product and target market? 3. What are are funding sources , good investors for such initiative? 4. Philps NL, they buyer for Chempaq whats up with them? Why did they not release it? 5. Are there any vendors we can still tie up with? 6. What is good penetration strategy? 7. We lack an electronics guy so should we collaborate. What should be the offering for Prof Morgan. Prof Morgan worked on Chempaq when Philips collborated with him. What should offering to him . 8. Any suggestions to succeed in this field Dr Ulrik( focus on technical challenges, Prof Morgan and Prof Kumaran's work, share with him every thing you can ; he was the pioneer in microflow cytometer) 1. Ask about his journey ? Where did he miss ? Top mistakes he should have avoided 2. Potential manufacturers . See we are looking at an integrated chip where the manufacturer fills/assembles the reagents too in the chips and give it us. 3. How to validate the results? What are the benchmarks ? 4. CE or FDA approval. How does one apply for them. 5. What was their strategy ,how many iterations vs how many chips. I mean we cannot place an order for million chip so what is the strategy? 6. His views on Prof Morgan's work. We lack an electronics guy so should we collaborate. What should be the offering for Prof Morgan 7. Please remember Ulrik used constant DC current and sensed change in voltage. In our case we are using AC voltage and sensing change in current. The change in current is measured using difference in amplitude of current from a reference pair minus measuring electrode ( we are diubg DIFFRENTIAL sensing). The order of change in current is in 'nano ampere' for a 1um particle in a 40umx40um side facing electrode. This output will be signal conditioned and then locked in to read the value. We are not yet started electronics but exhaustive work has been done by Morgan et al . This technique is called Microfludic Impedence Cytometery

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Page 1: Denmark

Soren ( focus on financials, investments,potential collaboration)

1. Why did they go bankrupt? their mistakes which we should not repeat?2. How much valuation can we expect with our team ,our product and target market?3. What are are funding sources , good investors for such initiative?4. Philps NL, they buyer for Chempaq whats up with them? Why did they not release it?5. Are there any vendors we can still tie up with?6. What is good penetration strategy?7. We lack an electronics guy so should we collaborate. What should be the offering for Prof Morgan. Prof Morgan worked on Chempaq when Philips collborated with him. What should offering to him . 8. Any suggestions to succeed in this field

Dr Ulrik( focus on technical challenges, Prof Morgan and Prof Kumaran's work, share with him everything you can ; he was the pioneer in microflow cytometer)

1. Ask about his journey ? Where did he miss ? Top mistakes he should have avoided2. Potential manufacturers . See we are looking at an integrated chip where the manufacturer fills/assembles the reagents too in the chips and give it us.3.  How to validate the results? What are the benchmarks ? 4. CE or FDA approval. How does one apply for them.5. What was their strategy ,how many iterations vs how many chips. I mean we cannot place an order for million chip so what is the strategy?6. His views on Prof Morgan's work. We lack an electronics guy so should we collaborate. What should be the offering for Prof Morgan7. Please remember Ulrik used constant DC current and sensed change in voltage. In our case we are using AC voltage and sensing change in current. The change in current is measured using difference in amplitude of current from a reference pair minus measuring electrode ( we are diubg DIFFRENTIAL sensing). The order of change in current is in 'nano ampere' for a 1um particle in a 40umx40um side facing electrode. This output will be signal conditioned and then locked in to read the value. We are not yet started electronics but exhaustive work has been done by Morgan et al . This technique is called Microfludic Impedence Cytometery

Please ask how did he do the platelet counting without using differential electrodes.8. IN our case we are diffrentiating among WBCs using mixed frequency AC . At high frequency(2Mz) the capcitance  dominates and at low frequency(0.5Mz) frequency resistance. How did he diffrentiated using resistance only ( he did not use AC)

9. How to make a <0.25$ cartridge

10)Did they had any collboration with University? I mean did he used to pay for the scientific literatures ? Since at IISc we have free download right. I think this might be a point for expenditure.

11) what were his Major expenses?

Page 2: Denmark

Dr Jacques ( focus on cartridge making ,our design strategy, 3 D electrodes, please do not tell him we are going for Ron, he will be disappointed, he sees business with us and we will see his expertise)

1. Share with our layer stacking and seek his expertise2. From where can get an integrated chip manufacturer.Does he think China is a good place. WHich country in europe is best to check out3. About 3D electrodes issue4. What are we missing in the design philosophy for cartrdige5. How can we bond PDMS and PMMA. PDMS and metal6. What should be the design philosophy for the instrument, how should the cartridge be inserted.7. What all companies is he aware of who are doing the same8. How to make a 0.25$ cartridge9.  about flexible printing for electrodes. He will for sure know some people.10. Do ask about Denmark-India proposal. Incase you are missing a copy then

Please feel free to ask as many questions as you can. I think for Ulrik and Jacuqes you should show them the video which I sent you, incase you need to download here is the link http://www.sendspace.com/file/34l03f .Also ,do take a printout of the literature 'integrated electronics' one

P.S. I am not sure if you should share any one about your meeting with others. What do you suggest?