Laboratory of Cancer Biology and Cannabinoid Research

Technion

David (Dedi) Meiri

Cannabidiolic acid, a major cannabinoid in fiber-type cannabis, is an inhibitor of MDA-MB-231 breast cancer cell migration. Takeda S, Okajima S, Miyoshi H, Yoshida K, Okamoto Y, Okada T, Amamoto T, Watanabe K, Omiecinski CJ, Aramaki H.

Cannabidiolic acid-mediated selective down-regulation of c-fos in highly aggressive breast cancer MDA-MB-231 cells: possible involvement of its down-regulation in the abrogation of aggressiveness. Takeda S, Himeno T, Kakizoe K, Okazaki H, Okada T, Watanabe K, Aramaki H.

leukaemiaAcute lymphoblastic

(ALL) (AML) leukaemiaAcute myeloid

Anal cancer Bile duct cancer Bladder cancer

Bone cancer Bowel cancer

tumoursBrain Breast cancer

Cancer of unknown primary Cancer spread to bone Cancer spread to brain Cancer spread to liver Cancer spread to lung

Carcinoid Cervical cancer

Children's cancer Choriocarcinoma

leukaemiaChronic lymphocytic (CLL)

(CML) leukaemiaChronic myeloid Colon cancer

Colorectal cancer Endometrial cancer

Eye cancer Gallbladder cancer

Gastric cancer tumoursGestational trophoblastic

(GTT) leukaemiaHairy cell

Head and neck cancer

Hodgkin lymphoma Kidney cancer

Laryngeal cancer Leukaemia

Liver cancer Lung cancer Lymphoma

Melanoma skin cancer Men's cancer

Mesothelioma Molar pregnancy

Mouth and oropharyngeal cancer Myeloma

Nasal and sinus cancers Nasopharyngeal cancer

Non Hodgkin lymphoma (NHL) cancer Oesophageal

Ovarian cancer Pancreatic cancer

Penile cancer Prostate cancer

Rare cancers Rectal cancer

Salivary gland cancer Secondary cancers

Skin cancer (non melanoma)

Soft tissue sarcoma Stomach cancer

Testicular cancer Thyroid cancer

Unknown primary cancer Uterine cancer Vaginal cancer

cancer Vulval Womb cancer

Women's cancers

CannaCollage – Israel’s strains Dedi Meiri, Ph.D. Assistant Professor | Faculty of Biology Laboratory of Cancer Biology and Cannabinoid Research

Teva Adir

Seach

IMC Canndoc

Sheifa Lachaim Pharmocann

Better

Tikun Olam

DMSO Extract1 THC CBD

Extract2 CBD THC

Extract3 CBD THC

רפאל משולם

Different extract from CBD high cannabis plant

Cont CBD1 CBD2 CBD3 CBD4 CBD5 CBD6 CBD7

Tonic-clonic seizures

ImageXpress micro (IXM) analysis

The effect of pure Δ9THC on cancer cell survival

A375 melanoma cell line

THC µ g/ml %

D e

a d c

e l l s

2 4 8

0

20

40

60

80

100 A375

A375 melanoma cell line

U-87 cell vability48 Hours from treatment

CNB1CNB2

CNB3CNB4

CNB6CNB8

0

25

50

75

100

125

4 ug/ml

Cannabis Extract

cell

viab

ility

(% o

f con

trol

)

PC3- cell viability48 Hours from treatment

CNB1CNB2

CNB3CNB4

CNB6CNB8

0

25

50

75

100

125

4ug/ml

cannabis extract

cell

viab

ility

(% o

f con

trol

)

1 2 3 4 5 6

glioblastoma Prostate cancer

1 2 3 4 5 6

Conversion of THCA to THC

O

COOH

OH

CH3

CH3

CH3

CH3 O

OH

CH3

CH3

CH3

CH3

to or h

- CO2

Tetrahydrocannabinolic acid, "inactive" (THCA)

C22H30O4, MW = 358.47

transTetrahydrocannabinol, "active" (THC)

C21H30O2, MW = 314.46

O

OH

CH3

CH3

CH3

CH3

Cannabinol,"slightly active" (CBN),

C21H26O2, MW = 310.43

- 4H

41

U-87 cell vability48 Hours from treatment

CNB1CNB2

CNB3CNB4

CNB6CNB8

0

25

50

75

100

125

4 ug/ml

Cannabis Extract

cell

viab

ility

(% o

f con

trol

)

PC3- cell viability48 Hours from treatment

CNB1CNB2

CNB3CNB4

CNB6CNB8

0

25

50

75

100

125

4ug/ml

cannabis extract

cell

viab

ility

(% o

f con

trol

)

1 2 3 4 5 6

glioblastoma Prostate cancer

1 2 3 4 5 6

No mutation Mutation 50% activity Mutation 30% activitv Mutation no activity

DATA BASE PROJECT

Optimize personalized Cannabis treatment

Cannabis strain database

Mouse models

Clinical Trails

Patient database Cannabis growers: Personalized strain

usage history

Foreigner clinics

Smart Phone - App4Cannabis:

Report symptoms and view optimized treatments

Aim 2

Aim 1

Mass-spec based metabolomics profiling

Computational analysis via machine learning

Aim 3 Lab research on therapeutic potential

of specific Cannabis strains

Clinical trials

Maximizing therapeutic efficacy while minimizing side effects

Aim 4

Optimize personalized Cannabis treatment

Cannabis strain database

Mouse models

Clinical Trails

Patient database Cannabis growers: Personalized strain

usage history

Foreigner clinics

Smart Phone - App4Cannabis:

Report symptoms and view optimized treatments

Aim 2

Aim 1

Mass-spec based metabolomics profiling

Computational analysis via machine learning

Aim 3 Lab research on therapeutic potential

of specific Cannabis strains

Clinical trials

Maximizing therapeutic efficacy while minimizing side effects

Aim 4

Reducing anxiety and violence in autistic kids

Reducing anxiety and violence in autistic kids

Reducing anxiety and violence in autistic kids

Optimize personalized Cannabis treatment

Cannabis strain database

Mouse models

Clinical Trails

Patient database Cannabis growers: Personalized strain

usage history

Foreigner clinics

Smart Phone - App4Cannabis:

Report symptoms and view optimized treatments

Aim 2

Aim 1

Mass-spec based metabolomics profiling

Computational analysis via machine learning

Aim 3 Lab research on therapeutic potential

of specific Cannabis strains

Clinical trials

Maximizing therapeutic efficacy while minimizing side effects

Aim 4

CK Sience

MEIRI’S LAB