SUPPLEMENTARY INFORMATION

Cyano-Bridged Coordination Polymer Nanoparticles as High

Relaxivity Contrast Agent for MRI.

Yannick Guari,a Joulia Larionova,a* Maurizio Corti,b,d Alessandro Lasciafari,c,d*

Massimo Marinone,c Giulio Poletti,c Karine Molvinger,e and Christian Guérin.a

aInstitut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, Chimie

Moléculaire et Organisation du Solide, Université Montpellier II, Place E. Bataillon,

34095 Montpellier cedex 5, France. Fax: (33) 4 67 14 38 52, e-mail: joulia@univ-

montp2.fr.

b Dipartimento di Fisica “A. Volta”, Università degli studi di Pavia, Via Bassi 6, I-27100

Pavia, Italy. Fax: (39) 0382 987563, email : corti@fisicavolta.unipv.it

c Istituto di Fisiologia Generale e Chimica Biologica, Università degli studi di Milano, I-

20134 Milano, Italy and CNR-INFM-S3 NRC, I-41100 Modena, Italy. Fax: (39) 02 50315775,

email : alessandro.lascialfari@unimi.it

d INFM-CNR c/o Dipartimento di Fisica “A. Volta”, Università degli studi di Pavia, Via

Bassi 6, I-27100 Pavia, Italy

e Institut Charles Gerhardt Montpellier, UMR 5253, Matériaux Avancés pour la Catalyse et la

Santé, Ecole Nationale Supérieure de Chimie de Montpellier, 8, rue de l’école normale,

34296 Montpellier cedex 5, France.

Electronic Supplementary Information for Dalton TransactionsThis journal is © The Royal Society of Chemistry 2008

H O

H

NH2H

CH2OH

OH O

H O

H

NHCOCH3H

OH

CH2OH

OO

n

Figure 1S. a) Chitosan chemical formula and b) Schematic representation of the intrapore

growth of cyano-bridged coordination polymer nanoparticles Gd3+/[Fe(CN)6]3-/chitosan.

a)

b)

Gd3+

[Fe(CN)6]3-

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0 5 10 15 20 25

χ' /

emu

μL-1 χ'' / em

u μL-1

T / K

0

5 104

1 105

1.5 105

2 105

2.5 105

3 105

0 50 100 150 200 250 300 350

1/χ

wei

ght /

emu-1

Oe-1

g

T / K

Figure 2S. Temperature dependence of the ac susceptibility, its χ’ (in phase) and χ’’, out-of-

phase components at the frequency of 125 Hz showing the paramagnetic behaviour of the

sample. Static field is of 0 Oe and alternative field is of 3 Oe. . b) Temperature dependence of

the inverse of the magnetic susceptibility performed with an applied field of 1000 Oe for the

sample 1.

0 2 4 6 8 100

50

100

150

200

250

Occ

uren

ce/n

Diameter/nm

Figure 3S. a) TEM image and b) size distribution of sample 1. Scale bar = 50 nm.

a)

b)

100

10

20

30

40

50

r 2p (m

M-1s-1

)

ν (MHz)

Figure 4S. Transverse relaxivity of sample 2 ( ), collected at T≈25°C, compared to the same

quantity reported for the commercial compound Omniscan ( ).

Equation 1S :

By definition of relaxivity rip (i=1 refers to longitudinal relaxivity, i=2 to transverse relaxivity): rip = [ (1/Ti)meas – (1/Ti)dia ] / c i=1,2 (1S) where (1/Ti)meas is the measured value on the sample with concentration c (mmol L-1) of magnetic center (8 mmol L-1 in our case), and (1/Ti)dia refers to the nuclear relaxation rate of the diamagnetic host solution (water in our case).