CURRICULUM VITAENatalia Sergeenko

Fish Pathologist/Bacteriologist, Ph.D.

PRESENT POSITION

Senior Scientist Laboratory of Fish and Invertebrates Health The Kamchatka Research Institute of Fisheries and Oceanography Federal Agency of FisheriesNaberezhnaya, 1868300 Petropavlovsk-KamchatskiyRUSSIA Phone: +7 415 31623 37 Mobile: +7 962 292 69 68E-mail: nvsergeenko@gmail.com

nvsergeenko@mail.ru

PERSONAL DETAILS

Born October 6, 1967 in Elizovo, Kamchatka, Russia, USSR. Russian citizen, female, single. Passport # 64№5047762

EDUCATION QUALIFICATIONS

Ph.D. in Ichthyology from the Kamchatka Institute of Fisheries and Oceanography & Kamchatka Government Technical University (Federal Agency of Fisheries), November 2012, Petropavlovsk-Kamchatskiy, Russia.

M.Sc. in Veterinary Medicine (Doctor of Veterinary Medicine), June 1989. Moscow Academy of Veterinary Medicine, Moscow, USSR.

EDUCATION

1984-1989: Faculty of Veterinary Medicine, Moscow Academy of Veterinary Medicine, Moscow, USSR.

LANGUAGE SKILLS

Russian – native.English – good.

WORK EXPERIENCE

September 1998-To present

Federal Agency of FisheriesThe Kamchatka Research Institute of Fisheries and Oceanography

Laboratory of Fish and Invertebrates Health Junior Scientist/Scientist/Senior Scientist

Duties: diagnosis of bacterial diseases and the isolation of pathogens in hatchery-reared fish; investigation of epizootic situations in natural reservoirs; determination of bacterial pathogens in crabs; investigation of pathogens affecting the commodity quality of fish.

March 2010 –October 2010

World Wildlife Fund (WWF), Kamchatka OfficeConsultant

Duties: development of an educational program for the Kamchatka Government Technical University (KGTU) on behalf of the WWF in order to introduce sustainable fishing methodology into the course curriculum of the College of Water Biological Resource’s degree programs.

August 1989 – March 1995

Sumskaya Biological Factory, Sumi, Ukraine,Microbiologist

Duties: preparation of vaccines against swine fever and Teshena’s disease.

RESEARCH INTERESTS

The taxonomy and classification of bacteria in fish. Disease pathogenesis, pathogen detection, diagnosis and control of bacterial fish diseases in hatcheries. Investigation of the genetic differences between species of bacteria from salmon in different natural reserves. The causes behind differences in prevalence of bacteria in fish in different reserves and years. Study of causes of shell disease in crabs. Analysis of micro flora of salmon spawning reserves.

TECHNICAL AND RELATED SKILLS

Preparation of Media: Create appropriate environments for the growth of microorganisms.

Collecting Samples of hydrobionts (fish, crabs and mussels): Bio-analysis, visual examination for pathology, bacteriological inoculation from organs and injuries.

Collecting Samples of Water: Direct and deep inoculation and filtration.

Isolation and Identification of Microorganisms: Investigation of the morphology, cultural and biochemical properties of bacteria using classic methods and test systems (BioMerieux and Lachema). Identification of the taxonomic position of isolated bacteria and the investigation of their sensitivity to antibiotics.

Experimental Inoculation: Direct inoculation of bacterial pathogens into live fish and subsequent recorded observations.

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Equipment: laminar flow cabinet, microscope, thermostatic incubator, autoclave, dry sterilizer, PH meter, electronic scale, centrifuge, homogenizer, photoelectric densitometer, colony counter, self-filling transfer pipette.

Programming for processing data: MS Office, STATISTICA, statistics software IBM SPSS.

Field Work: Participated in over 30 field investigations to remote areas in Kamchatka in order to take samples from hydrobionts and water.

Experienced in scientific report writing on an international level.

Interpersonal: Highly developed communications and team skills.

RESEARCH CRUISES EXPERIENCE

Participated in 2 scientific cruises in the Bearing Sea. Positions in cruises: Fish Pathologist.

TRAININGS

Courses on Bacterial Fish Diseases, Moscow Academy of Veterinarian Medicine, Certificate of Microbiologist, February 2011.

MAJOR PRESENTATIONS

III International Conference “Problems of Pathology Immunology and Preventative Health in Fish and other Hydrobionts”, Borok, Russia, July, 2011. PRESENTATION: “Pathogens of different etiology in pink salmon of the Kamchatka”.

II Bilateral Conference “Aquatic and Marine Animal Health”, Shepherdstown, West Virginia, USA, September, 2003. PRESENTATION: “Characterization of Aeromonas salmonicida subsp. salmobicida isolated from salmon spawned in hatcheries in Kamchatka”.

MAJOR SELECTED PUBLICATIONS (total – 27)

2011Ustimenko, E. A. & Sergeenko, N. V. Cause of Furunculosis Aeromonas salmonicida in spawning sockeye salmon (Oncorhynchus nerka Walbaum) in Azabachye Lake. Fisheries, V.12, 3 (47), 2011, 576-586.The paper described the results of the bacteriological investigation of spawning sockeye salmon from Azabachye lake in 2004-2009. The A. salmonicida that we isolated in Azabachye lake shared some of the peculiarities inherent to the bacteria of this species isolated from fish in reservoirs of the Asian coast of the Pacific Ocean before. However, some of the characteristics of the bacteria we isolated from fish in Azabachye lake were unique to this lake. Pathogenesity of the isolated bacteria A. salmonicidа showed its potential danger for salmon populations.

2007Sergeenko, N. V. & Nadeeva O.A. Contamination of Pacific salmon in Kamchatka with pathogens affecting the commodity quality of fish. Fishery Issues, 8, 3(31), 2007, 547-558.

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The comparative analysis of the contamination of Pacific salmon pathogens, their influence on commodity quality and their epidemiological value from research between 1990-2006 was carried out. The kinds of salmon having the greatest epidemiological value for the distribution of anisakiosis and difillobothriosis on Kamchatka peninsula were determined.

2005Sergeenko, N. V. & Ustimenko, E. A. Characterization of strains of Aeromonas salmonicida subsp. salmonicida isolated from spawning salmon in fish hatcheries on Kamchatka. In: Health and Diseases of Aquatic Organisms: Bilateral Perspectives R.C. Cipriano, I.S. Shchelkunov (Eds.), 2005, 256-259.Incidents of carriers of Aeromonas salmonicida among spawning salmon populations were detected in 2001-2002. Somewhat “atypical” characteristics were similar to reports of atypical strains isolated in Japan. In order to test the pathogenicity of the strain, an isolate from chum salmon was inoculated into fingerlings.

2000Sergeenko, N. V., Ustimenko, E. A. & Pugaeva, V. P. Determination of the pathogenicity of the vibrions, isolated from pink salmon, Oncorhynchus gorbuscha (Walbaum) in Karaginsky gulf (Kamchatka). Investigation of the biological water resources of the Kamchatka and the Northwest part of the Pacific ocean, Kamchatka Research Institute of Fisheries and Oceanography V22, 2000, 185-187.In 1997-1998 in Karaginsky Bay in the Bearing Sea a highly pathogenic strain of Vibrio anguillarun was isolated from diseased pink salmon. Using the immersion method, it was determined that 76% of the fry died and 80% were infected.

January 10, 2013 Natalia Sergeenko

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