curriculum vitae personal records · 6. member of the ph. d. advisory committee for sneha sundaram,...

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CURRICULUM VITAE

PERSONAL RECORDS

Name : Shyamal K. Roy

Nationality : USA

Address: (Home) : 2645 N. 160th Avenue

Omaha, NE 68116

: (Office) : Departments of OB/GYN and Cellular

and Integrative Physiology, DRC 8042

984515 Nebraska medical Center

Omaha, NE 68198-4515

Tel: (402)-559-6163

Fax: (402)-559-6164

ACADEMIC RECORDS

Year Degree Obtained Educational Institution

1973 B. Sc. (Physiology) City College, University of Calcutta, India

1975 M. Sc. (Physiology) University of Calcutta, India

1980 Ph. D. (Reproductive All India Institute of Medical Sciences,

Endocrinology) New Delhi, India

Continued Education

1987 Certificate in Molecular American Type Culture Collection,

Biology (NIH Subsidiary), Rockville, MD

1993 Certificate in Cell Life Technologies, Germantown, MD

Signaling

1998 Education Workshop UNMC (based on Dean’s nomination)

ACADEMIC EXPERIENCES

Year Status Educational Institutions

1976-77 Lecturer Department of Physiology, City College,

University of Calcutta, India

1980-81 Post-doctoral Fellow Department of Physiology, All India

Institute of Medical Sciences, New Delhi,

India

1981-82 Instructor Department of Physiology, All India

Institute of Medical Sciences, New Delhi,

India

1982-85 Post-doctoral Fellow Department of Physiology, University of

Kansas Medical Center, Kansas City, KS

1985-89 Research Assistant Department of Physiology, University of

Professor Kansas Medical Center, Kansas City, KS

1990-94 Assistant Professor Departments of Obstetrics & Gynecology

and Cellular and Integrative Physiology

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University of Nebraska Medical Center

Omaha, NE

1994-99 Associate Professor Same as above

1998 Tenure Same as above

1999-present Professor with tenure Same as above

HONORS

1. Junior Research Fellow of Indian Council of Medical Research, Govt. of India.

2. Post-doctoral Fellow of Family Planning Foundation of India.

3. Rockefeller Foundation International Post-doctoral Research Scientist.

4. Invited speaker to California Primate Research Center, Davis, CA.

5. Invited speaker to 2nd Asian and Oceanian Congress on Physiological Sciences, New

Delhi, India (1992).

6. Invited speaker to the USDA Institute at Clay Center, Nebraska.

7. Invited speaker to the Department of Animal Sciences, University of Nebraska, Lincoln.

8. Invited speaker to the Missouri-Kansas Reproductive Biology Symposium.

9. Invited speaker to International Symposium on “Prospects of zona pellucida glycoproteins

for immunocontraception,” New Delhi, India (1995).

10. Invited speaker to a NICHD forum on current status on ovarian regulation

11. Invited speaker to the Department of Pharmacology, UNMC

12. Invited speaker to the Department of Biochemistry and Molecular Biology, UNMC

13. Invited speaker to the Department of Cell Biology and Anatomy, UNMC

14. Invited speaker to the Department of OB/GYN, University of Ghent, Belgium

15. Invited speaker to the International Workshop on Early Folliculogenesis and Oocyte

Development: Basic and Clinical Aspects held at Royal College of London by Ares-

Serono Foundation

16. Invited speaker to the Department of Integrated and Molecular Physiology, University of

Kansas Medical Center, Kansas City, KS, 2002

17. Invited speaker to the Center for Reproductive Biology and Department of Pediatrics,

University of Michigan, Ann Arbor, Michigan 2003

18. Recipient of the Golden Forceps Award of the Department of OB/GYN, UNMC, 2005.

19. Keynote speaker (Osborn memorial Lecture), The Gilbert S. Greenwald Symposium on

Reproduction, Kansas City, MO, 2005

20. Recipient of the UNMC Distinguished Scientist Award for 2007

21. Invited speaker to Donald C. Johnson Lecture, Department of Integrated and Molecular

Physiology, University of Kansas Medical Center, Kansas City, KS, 2009

22. Invited speaker to department of genetics, Cell Biology and Anatomy, UNMC-2010

23. Invited speaker to department of Biology, Wichita State University, 2012.

MEMBERSHIP IN SCIENTIFIC SOCIETIES

1. Society for the Study of Reproduction, USA.

2. Endocrine Society, USA.

3. Society for the Study of Fertility, U. K.

4. American Association for the advancement of Science, USA.

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ADMINISTRATIVE RESPONSIBILITIES

1. Ad hoc Reviewer ---- Biology of Reproduction

Endocrinology

Molecular and Cellular Endocrinology

Journal of Histology

Human Reproduction

Molecular Human Reproduction

Reproduction

American Journal of Physiology

2. Ad hoc member -- The Welcome Trust Research Grant review panel, U. K.

3. Ad hoc reviewer -- USDA review panel for Programs on Reproductive Biology

4. Ad hoc member -- NIH panel to discuss the present status and formulate the future direction

of research in ovarian biology, 1986.

5. Ad hoc member -- NIH panel to discuss the emerging roles of cytoskeleton in Reproductive

Endocrinology.

6. Ad hoc member -- MBRS Study Section on Neurobiology and Physiology, NIH, 2002.

7. Ad hoc member -- Reproductive Endocrinology Study Section, NIH (2002, 2003)

8. Ad hoc Member -- Seed Research Grant Review Committee, UNMC

9. Member -- Integrated Clinical Endocrinology and Reproduction Study Section,

NIH (2005-2006)

10. Ad-hoc Member -- RFA entitled “Fertility preservation research: advancing beyond

technology” review panel-2010

11. Chairperson -- NICHD Special Emphasis Panel Study Section (2006)

12. Member -- Reproduction, Andrology and Gynecology Study Section, National

Institute of Child Health and Human Development-2010-2014

13. Member --UNMC Medical Curriculum Sub-Committee (Core IV, Endocrinology)

10. Member --Graduate Committee of the Department of Cellular and Integrative

Physiology, UNMC (2000 to 2006)

11. Member --Search Committee for the section Chief of Endocrinology, Department

of Internal Medicine

12. Member --Library Upgrade Committee, UNMC

13. Member --Education Committee, Society for the Study of Reproduction, 1998-2000

14. Member --Executive Committee of the Nebraska Physiological Society (APS

Chapter)-1998

15. Member -- Steering Committee of the Nebraska Physiology Society-1998

16. Member -- Editorial Board, Biology of Reproduction (2001-2004)

17. Member -- UNMC promotion and Tenure Committee (2004-2010)

18. Coordinator --Work-in-progress meetings for Basic Science Research, Department of

OB/GYN (1994-1996)

19. Course Director --Reproductive Endocrinology (Physiology 914), Department of Cellular

and Integrative Physiology, UNMC

20. Member -- Comparative Medicine Advisory Group-UNMC-2004-present

21. Secretary

and Treasurer --Nebraska Physiology Society (1999- 2001)

22. Chairperson -- Education Committee, Society for the Study of Reproduction-2000

23. Director -- Molecular Endocrinology Laboratory

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24. Interim Director -- Reproductive Endocrinology/Infertility, Department of OB/GYN

25. President -- Nebraska Physiological Society (2002-2003)

26. Chairperson -- Promotion and Tenure Committee, Department of OB/GYN, UNMC,

2001-2008

27. Director -- Molecular Biology Core, Department of Cellular and Integrative

Physiology, 2001-2013

28. Chairperson -- Local Arrangement Committee for 2006 Annual meeting of the Society

for the Study of Reproduction

29. Member -- Research and Development Committee, College of Medicine, UNMC,

2005-2011

30. Member -- Editorial Board of the Molecular and Cellular Endocrinology-2009 to

2014

31. Member -- Future meeting site committee, Society for the Study of Reproduction,

2008-2010

32. Member -- Editorial Board of the Hormonal Studies-2012 to present

33. Member -- Editorial Board of Scientific Reports (Nature Publication)-2017 to present

34. Co-leader -- Endocrinology curriculum development committee, UNMC new medical

Curriculum

35. Co-leader -- GU/GYN curriculum development committee, UNMC new medical

Curriculum

Graduate Advisory Committee

1. Member of the Ph. D. Advisory Committee for Constance T. Moore, Department of

Anatomy and Cell Biology, 1994-1998

2. Chair of the Ph. D. Advisory Committee for James R. Schwartz, OB/GYN through MSIA,

3. Chair of the MS Advisory Committee for Ni Yu, Department of Biology, University of

Nebraska at Omaha

4. Member of the Ph. D. Comprehensive Examination Committee for S. Nuti, Department of

Biochemistry and Molecular Biology

5. Chair of the Ph. D. Advisory Committee for Y-Ming Zhang, Department of Cellular and

Integrative Physiology, 2001-2004

6. Member of the Ph. D. Advisory Committee for Sneha Sundaram, Department of

Pharmaceutical Sciences

7. Member of the Ph. D. Advisory Committee for Edward Arvasias, Department of

Pharmacolgy and Neuroscience

8. Chair of the Ph. D. Advisory Committee for Anindit Mukherjee, Department of Cellular and

Integrative Physiology, 2007-2012

9. Member of the Ph. D. Advisory Committee for Dulce Maroni, Department of Biochemistry

and Molecular Biology

10. Chair of the Ph. D. Advisory Committee for Prabuddha Chakraborty, Department of Cellular

and Integrative Physiology, 2010-present

Teaching

1. Endocrinology in Core 4 of M-1

2. Team based Learning-M1

3. Problem based learning- Core 4 of M-1 (2001-2002)

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4. Small Group Teaching-M2

5. Grand rounds in the Department of OB/GYN

6. Graduate course in the Department of Cellular and Integrative Physiology (1995-1998)

7. Nursing course in the Department of Cellular and Integrative Physiology (1995-2000)

8. 606/806 Course in the Department of Cellular and Integrative Physiology

9. Reproductive Endocrinology Journal Club in the Department of OB/GYN (1996-1998)

10. Reproductive Endocrinology (Physiology 914) in the Department of Cellular and

Integrative Physiology

Mentoring students

1. Summer Student -- 1990 -- Christine L. Ogren, Buena Vista College, Iowa

(recipient of the UNMC Alumni Summer Fellowship).

Presently a Physician

2. Summer Student -- 1991-- Andrew Kouba, Northwest Missouri College, MO (selected to

present his research in the National Undergraduate Research

Forum held in March 1992 in Minnesota). Presently Assistant

Professor

3. Summer Student -- 1992 --Jennifer Hughes, University of Nebraska, Omaha

4. Summer Student -- 1993-- Michael Bassik, University of Wisconsin. Presently post-

doctoral research fellow

5. Summer Student -- 1994-- Delores Terada, Creighton University, NE. Presently a

pharmacist

6. Summer Student -- 1994-- Sonal Saxena, High School senior, Nebraska. Presently a

Physician

7. Resident Research Fellow -- 1992-- Ann Sjulin, MD, OB/GYN, recipient of the Ortho

Award for best research paper in Resident Day, OB/ GYN.

Presently a OB/GYN practioner

8. Resident Research Fellow – 1997-2000—Brittani Antisdel, MD, OB/GYN. Presently a

OB/GYN practioner

9. M. S. Student -- Ni Yu -- Biology (UNO)—completed in March 1998. Presently

Researcher at a Pharmaceutical Compnay in San Francisco

10. Ph. D. Student-- James Schwartz --- OB/GYN (MSIA)-completed in 2000. Presently

Director of Assisted Reproductive Endocrinology laboratory, St. Louis,

MO.

11. Ph. D. Student—Robert Ostronic— Cellular and Integrative Physiology

12. Ph. D. Student— Yi-Ming Zhang —Completed in 2004- Cellular and Integrative

Physiology, Present a Faculty in a University in Shanghi.

11. Post-doctoral Research Associate – 1990-1991, Subhadra Ghosh, Ph. D., University of

Calcutta, India. Presently computer specialist.

12. Post-doctoral Research Associate -- Alok R. Kole, Ph. D., University of Calcutta, India.

Presently, Associate professor

13. Post-doctoral Research Associate –1999-2004- P. Yang, Ph. D., Zhejiang University, P. R.

China. Presently, Professor and Chair, Department of

Cell Biology and Anatomy, University of Louisiana at

Sreevpot.

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14. Post-doctoral Research Associate –2001-2004- J. R. Wang, Ph. D., Zhejiang University, P.

R. China. Presently, Post-doctoral Research Associate,

Baylor College of Medicine

15. Post-doctoral Research Associate –2007-2011, C. Wang, Ph. D., Zhejiang University, P.

R. China, presently Associate Professor, Ob/Gyn,

Massachusetts General Hospital, Harvard Medical

School, MA.

16. Post-doctoral Research Associate – 2008-2009, Liying Geng, Ph. D., Zhejiang University,

P. R. China

17. Summer Research Student- 1999 -- Amy Clark—M1, UNMC, Presently a physician

18. Summer Student-1999-Kristina Garnett—University of Nebraska at Kearney,

Presently a physician

19. Summer student-2000-Amy Guminski-University of Nebraska at Lincoln. Presently a

Physician

20. Summer student-2000-Micelle Dorsey, Nebraska Wesleyan University, Lincoln. Presently

a graduate student

21. Summer student-2003-Helen Gougeon, France.

22. Summer student-2004-Lindsay Ackerman, UNO.

23. Summer student-2006-Jean Huff, M1 Student, UNMC, currently a physician

24. Summer student-2007-Kristina Derrick, M1 student, currently a physician

25. Summer student-2008-Chelsea Newman, M1 Student, UNMC, currently a physician

26. Graduate student (Rotation)-Che Qi- Department of Cellular and Integrative Physiology,

UNMC. Presently doing residency program

27. Graduate student (Rotation)-Jennifer Green- Department of Cellular and Integrative

Physiology, UNMC. Currently a physician

28. Ph. D. Student—Anindit Mukherjee --- Department of Cellular and Integrative

Physiology, 2007-2012. Now a post-doctoral trainee in University of Pittsburgh.

29. Ph. D. Student-- Prabuddha Chakraborty-- Department of Cellular and Integrative

Physiology, 2009-2016. Now a post-doctoral trainee in the Institute of

Genetics, University of North Carolina, NC

RESEARCH INTERESTS AND EXPERIENCES

My research interests began with the study of the mechanism(s) of embryo implantation

and pregnancy recognition in laboratory mammals and in sub-human primates. Those studies

included in vitro culture of rat and mouse preimplantation embryos, histochemical and bio-

chemical studies of lysosomal hydrolases in embryo and uterus during pregnancy and

pseudopregnancy, biochemical studies on carbohydrate metabolic enzymes in rodents' embryos

and monkey uterus, and embryo transfer to foster mothers.

For the last 19 years, I have been working on the biology and endocrinology of ovarian

follicles and non-follicular compartments. More specifically, I am attempting to unravel the

intricate cellular and molecular changes that occur in growing follicles during their sojourn from

primordial to antral to ovulatory stages, and the modulatory roles of endocrine and paracrine

factors in these processes. For this purpose, I have developed a model, which allows, for the first

time to study directly the biology of intact preantral follicles in each stage of development and

the importance of this model has already been internationally recognized. Besides, this model

has already initiated an awareness to address follicular development from an entirely new

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dimension in a variety of species. Using hamster as an animal model, I have already shown the

roles of gonadotropins on follicular DNA replication and steroidogenesis, and documented the

presence of peptide receptors, in a series of publications. My current interests include the

understanding of the stage specific changes in follicular protein synthesis in signaling follicular

differentiation and its hormonal control, specific gene expression during granulosa cell

differentiation, molecular and cellular mechanisms of growth factors mediation of signal

transduction and subsequent intracellular events in follicular cells. We are looking into the cell-

type specific hormonally regulated expression of growth factor receptor messenger RNA

expression during follicular development. I have developed a long-term culture system that

supports the growth of intact antral follicles in vitro for several days. This model adds a new

dimension to studying the factors regulating follicular development, effects of xenobiotics on the

biology of follicular cells -- cellular and molecular aspects, expression of specific genes (e.g.

growth factor genes, etc.), proto-oncogenes and foreign genes introduced in vitro. We were the

first to develop a successful technique to dissociate intact preantral follicles from human ovaries

and a long-term culture system for their in vitro growth and differentiation. These latter

techniques will add significantly to the current understanding of the mechanism of endocrine and

autocrine/ paracrine regulation of human folliculogenesis. We are also studying the endocrine

regulation of epithelial-mesenchymal interaction in forming theca cells during follicular de-

velopment, and cell lineage in the developing ovary.

Currently, my laboratory focuses on understanding the mechanisms whereby estrogen

regulates the differentiation of granulosa cells, and promotes the formation and development of

primordial follicles.

GRANT SUPPORT

Grant application pending: R01 HD-038468 (Roy) 01/01/2015 – 12/31/2019 NIH/NICHD Primordial follicle formation: Role of estrogen The goal of this project is to identify molecular mechanism(s) involved in primordial follicle formation in the ovary Role in Project: PI Grant #: 1528415 NSF

Primordial follicle formation: Role of BMP2 and Estradiol-17 The goal of this project is to identify the role of BMP2 in primordial follicle formation. Role in the Project: PI

Past Research Support P01-HL062222 (Zucker) 07/01/2005-06/30/2014 DHHS/NIH/NHLBI Neurocirculatory function in chronic heart failure Core C - Molecular Biology, Biochemistry and Histology Core The goal of this core unit is to provide complementary molecular, cellular, biochemical and scientific support for the four research projects by carrying out a variety of biochemical, molecular biological and histological techniques.

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Role in Project: Core Director

SUB090218 (Davis) 04/01/2009-03/31/2014 DHHS/NIH/NICHD Aging Pituitary Gonadal Axis Project 2: Role of Glycosylation in FSH Signaling in FSH Target Cells To study the effects of di- and tetra-glycosylated isoforms of FSH on granulosa cells, intact ovarian follicles, and bone cells. Role in Project: Co-I

Roy 07/01/2013– 6/30/2014 UNMC Bridge Funding Follicular morphogenesis during perinatal development The goal of this project is to identify molecular mechanism(s) involved in primordial follicle formation in the ovary Role in Project: PI Roy/Wang 07/01/2013 – 6/30/2014 Olson Foundation Role of estrogen in Primordial follicle formation The goal of this project is to identify the mechanisms whereby membrane estrogen receptor mediates estrogen action during primordial follicle formation in the ovary Role in Project: Co-PI Padmanavan, P 07/01/2007-06/30/2009 NIH P01 Prenatal programming of reproductive Health and Disease Role in the Project: Co-I 31-3205-0852 (Roy) 07-01-2011-6-30-2011 NRI/UNMC Laser Capture Microdissection: Tissue-based translational research Equipment grant for the Imaging Core; Role in the project: PI

PUBLICATIONS

1. Sengupta J, Roy SK, Manchanda SK Hormonal control of implantation: A possible

lysosomal function in the embryo-uterus interaction. J Steroid Biochem 11: 729-744, 1979

2. Roy SK, Sengupta J, Manchanda SK Effect of a long-acting fluorinated androgen on

implantation in 'delayed' mice. Hormn Res 12: 22-31, 1980

3. Roy SK, Sengupta J, Manchanda SK Induction of implantation by androgen in mice with

delayed implantation. J Reprod Fertil 58: 339-343, 1980

4. Roy SK, Sengupta J, Manchanda SK Studies on embryonic estrogen induced growth and

differentiation of embryos in the mouse. In: Proc XXVIII International Congress on

Physiological Sciences, Budapest, p. 671, 1980

5. Roy SK, Sengupta J, Manchanda SK Rat embryo development in vitro. Acta Endocrinol 96:

546-551, 1981

6. Sengupta J, Roy SK, Manchanda SK Effect of anti-estrogen on implantation in the mouse. J

Reprod Fertil 62: 433-436, 1981

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7. Roy SK, Sengupta J, Paria BC, Manchanda SK Inhibition of trophoblast maturation and

expansion of rat blastocysts by estrogen antagonist in vitro. Acta Endocrinol 99: 129-135,

1982

8. Sengupta J, Roy SK, Manchanda SK The effect of an estrogen synthesis inhibitor, 1,4,6-

androstatriene-3, 17-dione on mouse embryo development in vitro. J Reprod Fertil 66: 63-

66, 1982

9. Roy SK, Sengupta J, Manchanda SK Histochemical study of -glucuronidase in the rat

uterus during implantation and pseudopregnancy. J Reprod Fertil 68: 161-164, 1983

10. Sengupta J, Roy SK, Paria BC, Manchanda SK Role of embryonic estrogen during early

gestation and implantation. Indo-US workshop on ovum implantation, ICMR Publication, p.

54-65, 1984

11. Roy SK, Greenwald GS An enzymatic method for dissociation of intact follicles from the

hamster ovary: Histological and quantitative aspects. Biol Reprod 32: 203-215, 1985

12. Roy SK, Greenwald GS Quantitative analysis of in vitro incorporation of [3H]thymidine into

hamster follicles during the estrous cycle. J Reprod Fertil 77: 143-152, 1986

13. Roy SK, Greenwald GS Effects of FSH and LH on incorporation of [3H]thymidine into

follicular DNA. J Reprod Fertil 78: 201-209, 1986

14. Roy SK, Wang SC, Greenwald GS Radioreceptor and autoradiographic analyses of FSH,

hCG and prolactin binding sites in primary to antral hamster follicles during the

periovulatory period. J Reprod Fertil 79: 307-313, 1987

15. Roy SK, Greenwald GS In vitro steroidogenesis by primary to antral hamster follicles during

the periovulatory period: Effects of follicle stimulating hormone, luteinizing hormone and

prolactin. Biol Reprod 37: 39-46, 1987

16. Roy SK, Greenwald GS In vitro effect of follicle stimulating hormone, luteinizing hormone

and prolactin on follicular deoxyribonucleic acid synthesis in the hamster. Endocrinology

122: 952-958, 1988

17. Roy SK, Greenwald GS Long term culture of preantral follicles and their conversion to early

antral stages. Biol Reprod 38 (Suppl): 436, 1988

18. Roy SK, Greenwald GS Deoxyribonucleic acid polymerase- activity in hamster follicles

during the estrous cycle: Roles of FSH and LH. Mol Cell Endocrinol 61: 157-166, 1989

19. Roy SK, Greenwald GS Hormonal requirements for the growth and differentiation of

hamster preantral follicles in long-term culture. J Reprod Fertil 87: 103-114, 1989

20. Roy SK, Greenwald GS Immunohistochemical localization of epidermal growth factor-like

activity in the hamster ovary with a polyclonal antibody. Endocrinology 126: 1309-1317,

1990

21. Roy SK, Greenwald GS Genetic differences in follicular DNA synthesis between two strains

of hamsters. Proc Soc Exp Biol Med 194: 136-143, 1990

22. Roy SK, Greenwald GS In vitro effects of epidermal growth factor, insulin-like growth

factor-I, fibroblast growth factor and follicle stimulating hormone on hamster follicular

deoxyribonucleic acid synthesis and steroidogenesis. Biol Reprod 44: 889-896, 1991

23. Wong XN, Roy SK, Greenwald GS In vitro DNA synthesis by isolated intact follicles from

the cyclic mouse. Biol Reprod 44: 857-863, 1991

24. Roy SK, Greenwald GS Mediation of follicle stimulating hormone action on follicular

deoxyribonucleic acid synthesis by epidermal growth factor. Endocrinology 129: 1903-1908,

1991

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25. Roy SK, Ogren CL, Roy C, Lu B Cell-type specific localization of transforming growth

factor-2 and transforming growth factor-1 in the hamster ovary: Differential regulation by

follicle stimulating hormone and luteinizing hormone. Biol Reprod 46: 595-606, 1992

26. Roy SK Involvement of tyrosine kinase in follicle stimulating hormone, cyclic AMP and

epidermal growth factor induced in vitro DNA synthesis in hamster preantral and early antral

follicles. Biol Reprod 46 (Suppl): 177, 1992

27. Roy SK Epidermal growth factor and transforming growth factor-modulation of follicle

stimulating hormone-induced deoxyribonucleic acid synthesis in hamster preantral and early

antral follicles. Biol Reprod 48: 552-557, 1993

28. Roy SK Transforming growth factor- potentiation of FSH-induced deoxyribonucleic acid

synthesis in hamster preantral follicles is mediated by a latent induction of epidermal growth

factor. Biol Reprod 48: 558-563, 1993

29. Roy SK, Treacy BJ Isolation and long-term culture of human preantral follicles. Fertil Steril

53: 783-790, 1993 PMID: 8458497

30. Roy SK Regulation of ovarian follicular development: A review of microscopic studies.

Microscopy Research and Techniques 27: 83-96, 1994 PMID: 8123910

31. Roy SK, Harris SG Antisense EGF oligonucleotide inhibits follicle stimulating hormone-

induced in vitro DNA and progesterone synthesis in hamster preantral follicles. Molecular

Endocrinology 8: 1175-1181, 1994 PMID: 7838150

32. Roy SK, Hughes J Ontogeny of granulosa cells in the ovary: Lineage specific expression of

transforming growth factor-2 and transforming growth factor-1. Biol Reprod 51: 821-

830, 1994 PMID: 7849185

33. Roy SK, Kole AR Transforming growth factor- receptor Type II (TRII) expression in the

hamster ovary: Cellular site(s), biochemical properties and hormonal regulation.

Endocrinology 136: 4619-4620, 1995 PMID: 7664681

34. Roy SK, Greenwald GS Follicular development through preantral stages: signalling via

growth factors. J Reprod Fertil, Suppl. 50: 83-94, 1996 PMID: 8984191

35. Roy SK, Greenwald GS Methods of separation and in vitro culture of preantral follicles

from mammalian ovaries. Human Reproduction Update 2: 236-245, 1996 PMID: 9079416

36. Roy SK, Carlson AM Gonadotropin regulation of transforming growth factor receptor

(TR) gene expression in hamster ovarian follicles. Biol Reprod 56 (suppl), 203, 1997

37. Roy SK, Carlson AM EGF regulation of follicular TGF- receptor gene expression.

Symposium on Modes of EGF Receptor Signaling, Proc of the 9th Annual Growth factor and

Signal transduction Conference, Iowa State University, p 11., 1997

38. Hill GE, Lackner RP, Roy SK, Sisson JH The impact of one lung ventilation on Qs/Qt and

atrial natruretic peptide release during thoracotomy. Am J Anesth 24: 232-235, 1997

39. Roy SK, Kole AR Ovarian transforming growth factor- (TGF- ) receptors: In vitro effects

of follicle stimulating hormone, epidermal growth factor and TGF- on receptor expression

in human preantral follicles. Molecular Human Reproduction 4: 207-214, 1998 PMID:

9570266

40. Roy SK, Kurz SG, Carlson AC, DeJonge CJ, Ramey JW, Maclin-Collins VM

Transforming growth factor beta receptor expression in hyperstimulated human

granulosa cells and cleavage potential of the zygotes. Biol Reprod 59:1311-1316,

1998 PMID: 9828172

41. McConnell PI, Cornish KG, Wang W, Roy SK, Zucker IH Effects of 17-estradiol on

coronary reactive hyperemic responses in conscious dogs. FASEB abstract., 1998

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42. Schwartz JR, Roy SK In vitro culture of hamster ovarian primary interstitial cells: Effect of

serum. Biol Reprod 59:1187-1194, 1998 PMID: 9780326

43. Roy SK, Terada DM Glucose metabolic activity of human preantral follicles: In vitro

modulation by follicle stimulating hormone (FSH), luteinizing hormone (LH), epidermal

growth factor (EGF), insulin-like growth factor-I (IGF-I) and transforming growth factor-1

(TGF-1). Biol Reprod 60: 763-768, 1999

44. Ni Yu, Roy SK Development of primordial and preantral follicles from undifferentiated

somatic cells and oocytes in the hamster prenatal ovary in vitro: Effect of insulin. Biol

Reprod. 61:689-712, 1999

45. Roy SK, Albee LR, Madhwa Raj HG Preantral follicular development in the hamster ovary is

modulated by gonadotropin. Ares-Serono Foundation International Workshop on Early

folliculogenesis and oocyte development: Basic and clinical aspects, London, UK., 1999

46. Harvell DME, Strecker TE, Tochacel M, Xie B, Pennigton KL, McComb RD, Roy SK, Shull

JD Rat strain-specific actions of 17-estradiol in the mammary gland: Correlation between

estrogen-induced lobuloalveolar hyperplasia and susceptibility to estrogen-induced

mammary cancers. Proc Natl Acad Sci USA 97:2779-2784, 2000

47. Roy SK. Regulation of TGF--receptor type I (TRI) and Type II (TRII) messenger RNA

expression in the hamster ovary by gonadotropins and steroid hormones. Biol Reprod

62:1858-1865, 2000 PMID: 10819793

48. Schwartz JR, Roy SK Expression of P450 side chain cleavage (CYP11A1) and P450 17-

hydroxylase-17/20-lyase (CYP-17) messenger ribonucleic acid in hamster primary interstitial

cells in vitro: Differential regulation of steroidogenesis by cyclic adenosine monophosphate.

Biol Reprod 63:503-507, 2000 PMID: 10906056

49. Roy SK, Albee LR Glucocorticoid modulates estrogen (E)- and progesterone (P)-induced

transforming-growth factor-receptor (TR) mRNA expression in hamster preantral

follicles. Biol Reprod 62:299, 2000

50. Yang P, Roy SK Stage-specific differential induction of transforming growth factor-

receptor (TR) mRNA by epidermal growth factor (EGF) in hamster ovarian follicles. Biol

Reprod 62:298, 2000

51. Schwartz JR, Roy SK Developmental expression of cytochrome P450 side-chain cleavage

(CYP11A1) and cytochrome P450 17-hydroxylase (CYP17) messenger ribonucleic acid

and protein in the neonatal hamster ovary. Biol Reprod 63:1586-1593, 2000 PMID:

11090424

52. Roy SK, Albee LR Requirement for follicle-stimulating hormone(FSH) action in the

formation of primordial follicles during perinatal ovarian development in the hamster.

Endocrinology 141:4449-4456, 2000 PMID: 11108254

53. Yang P, Roy SK Epidermal growth factor modulates transforming growth factor receptor

messenger ribonucleic acid and protein levels in hamster preantral follicles in vitro. Biol

Reprod 65:847-854, 2001 PMID: 11514350

54. Roy SK, Garnett K Hormonal regulation of epidermal growth factor receptor (EGFR) protein

and messenger RNA. Proceeding of the 34th Internatioanl Congress of Physiological

Sciences, Christchurch, New Zealand, 1692, 2001

55. Roy SK, Yang P Ontogeny and the role of eCG on the spatio-temporal and correlative

expression of SF-1, CYP11A1 and CYP17 in perinatal hamster ovaries. Ovarian Workshop,

Upper Hut, New Zealand, 2001

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56. Yang P, Roy SK Activation of cyclin D2/CDK4 is the primary mechanism whereby FSH

stimulates DNA synthsis in granulosa cells of intact preantral hamster follicles. Annual

Meeting of the Endocrine Scociety, San Francisco, 2002

57. Li YF, Roy SK, Channon KM, Zucker IH, Patel KP Effect of in vivo gene transfer on nNOS

in the PVN on renal nerve discharge in rats. Am J Physiol Heart Circ Physiology 282:H594-

601, 2002

58. Yang P, Kriatchko A, Roy SK Expression of estrogen receptor- (ER) and estrogen

receptor- (ER) in the hamster ovary: differential regulation by gonadotropins and ovarian

steroid hormones. Endocrinology 143:2385-2398, 2002 PMID: 12021204

59. Garnett K, Wang JR, Roy SK Spatio-temporal expression of EGF-receptor (EGFR)

messenger RNA and protein in the hamster ovary: Follicles stage-specific differential

modulation by follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol and

progesterone. Biol Reprod 67: 1593-1604, 2002 PMID: 12390893

60. Wang X, Wang H, Matsumoto H, Roy SK, Das SK and Paria BC. Dual source and target of

heparin-binding EGF-like growth factor during the onset of implantation in the hamster.

Development 129: 4125-4134, 2002

61. Roy SK, Wang JR, Yang P. Dexamethasone inhibits transforming growth factor – receptor

(TR) mRNA expression in hamster preantral follicles: possible association with NF-YA.

Biol Reprod 68:2180-2188, 2003 PMID: 12606399

62. Yang P, Roy SK. Follicle-stimulating hormone-induced DNA synthesis in the granulosa cells

of hamster preantral follicles involves activation of CDK4 rather than cyclin D2 synthesis.

Biol Reprod 70: 509-517, 2004 PMID: 14561638

http://www.ncbi.nlm.nih.gov/pubmed/14561638?dopt=Citation

63. Wang JR, Roy SK. Growth differentiation factor-9 (GDF-9) and stem cell factor (SCF)

promote primordial follicle formation in the hamster: Modulation by follicle-stimulating

hormone. Biol Reprod 70: 577-585, 2004 PMID: 14585807

64. Zhang YM, Roy SK. Down regulation of follicle-stimulating hormone receptor (FSHR)

messenger ribonucleic acid (mRNA) levels in the hamster ovary: effect of the endogenous

and exogenous FSH. Biol Reprod 70: 1580-1588, 2004 PMID: 14749302,


65. Yang P, Wang JR, Shen Y and Roy SK. Developmental expression of estrogen receptor-

(ER) and ER in the hamster ovary: Regulation by follicle-stimulating hormone (FSH).

Endocrinology 145: 5757–5766, 2004 PMID: 15345677,


66. Steckler T, Wang J, Bartol FF, Roy SK, and Padmanabhan V. Fetal programming: Prenatal

Testosterone Treatment Causes Intrauterine Growth Retardation, reduces ovarian reserve and

increases ovarian follicular recruitment. Endocrinology 146:3185-3193, 2005 PMID:

15802500, http://www.ncbi.nlm.nih.gov/pubmed/15802500?dopt=Citation

67. Li X, Li S, Xu Z, Lou MF, Anding P, Liu D, Roy SK, Rozanski GJ. Redox control of K+

channel remodeling in rat ventricle. J Mol Cell Cardiol 40:333-4, 2006. PMID: 16288907,


68. Wang C, Roy SK. Expression of Growth Differentiation Factor 9 in the Oocytes Is Essential

for the Development of Primordial Follicles in the Hamster Ovary. Endocrinology 147:

1725-174, 2006. PMID: 16384866,








13

69. Yang P, Roy SK. A novel mechanism of FSH regulation of DNA synthesis in the granulosa

cells of hamster preantral follicles. Involvement of a protein kinase C mediated MAP kinase

3/1 self-activation loop. Biol Reprod 75:140-148, 2006. PMID: 16525034,


70. Yang P, Roy SK. Transforming growth factor B1 stimulated DNA synthesis in the granulosa

cells of preantral follicles: Negative interaction with epidermal growth factor. Biol Reprod

75:149-157, 2006. PMID: 16525033,


71. Liu D, Gao L, Roy SK, Cornish KG, Zucker IH. Neuronal angiotensin II type I receptor

upregulation in heart failure: Activation of activator protein 1 and Jun N-terminal kinase.

Ciculation Research 99: 1004-1011, 2006,


72. Wang C, Roy SK. Development of primordial follicles in the hamster: Role of estradiol17.

Endocrinology 148:1707-1716, 2007,


73. Wang C, Prossnitz ER, Roy SK. Expression of G protein-coupled receptor 30 in the hamster

ovary: differential regulation by gonadotropins and steroid hormones. Endocrinology 148:

4853-4864, 2007. PMID: 17640985, http://www.ncbi.nlm.nih.gov/pubmed/17640985?dopt=Citation

74. Wang C, Roy SK. Hormonal regulation of bone morphogenetic protein receptor (BMPR)

expression in perinatal hamster ovary, and the effect of BMPs on the formation and

development of primordial follicles. Biol Reprod (Suppl), Abstract 280, 2007. 75. Hou X, Arvisais EW, Jiang C, Chen DB, Roy SK, Pate JL, Hansen TR, Rueda BR and Davis

JS. Prostaglandin F2α (PGF2α) stimulates the expression and secretion of TGFB1via

induction of the Early Growth Response 1 Gene (EGR1) in the bovine corpus luteum. Mol

Endocrinol 22:403-414, 2008 PMID: 17916653,


76. Liu D, Gao L, Roy SK, Cornish KG, Zucker IH. Role of Oxidant Stress on AT1 receptor

expression in neurons of rabbits with heart failure and in cultured neurons. Circ Res 103:

186-193, 2008 PMID: 18566341,


77. Wang C, Prossnitz ER and Roy SK. G protein-coupled receptor 30 expression is required

for estrogen stimulation of primordial follicle formation in the hamster ovary. Endocrinology

149: 4452-4461, 2008, http://www.ncbi.nlm.nih.gov/pubmed/18499747?dopt=Citation

78. Sundaram S, Roy SK, Kompella UB. Differential expression of LHRH-receptor in bovine

nasal tissue and its role in deslorelin delivery. Peptides. 2009 Feb;30(2):351-8. 2009 PMID:

18992782 http://www.ncbi.nlm.nih.gov/pubmed/18992782?dopt=Citation 79. Wang C, Roy SK. Expression of bone-morphogenetic protein receptor (BMPR) during

perinatal ovary development and primordial follicle formation in the hamster: Possible

Regulation by follicle-stimulating hormone. Endocrinology, 150: 1886-1896. 2009 PMID:

19074578, http://www.ncbi.nlm.nih.gov/pubmed/19074578?dopt=Citation

80. Sundaram S, Roy SK, Ambati BK, Kompella UB. Surface-functionalized nanoparticles for

targeted genedelivery across nasal respiratory epithelium. FASEB Journal, 23: 3752-3765,

2009 PMID: 19608628 http://www.ncbi.nlm.nih.gov/pubmed/19608628?dopt=Citation 80. Cleveland AG, Oikarinen SI, Bynote KK, Marttinen M, Rafter JJ, Gustafsson JA, Roy SK,

Pitot HC, Korach KS, Lubahn DB, Mutanen M, Gould KA. Disruption of estrogen receptor









http://www.ncbi.nlm.nih.gov/pubmed/18992782?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum

http://www.ncbi.nlm.nih.gov/pubmed/18992782?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum




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signaling enhances intestinal neoplasia in Apc(Min/+) mice. Carcinogenesis, 30: 1581-1590,

2009 PMCID: 2736300 http://www.ncbi.nlm.nih.gov/pubmed/19520794?dopt=Citation

81. Wang C, Roy SK. Expression of E-Cadherin and N-Cadherin in Perinatal Hamster Ovary:

Possible Involvement in Primordial Follicle Formation and Regulation by Follicle-

Stimulating Hormone. Endocrinology, 151: 2319-2330, 2010, PMID: 20219978


82. Alwis ID, Maroni DM, Hendry IR, Roy SK, May JV, Leavitt WW, Hendry WJ. Neonatal

Diethylstilbestrol Exposure Disrupts Ovarian Structure/Function Via Both Direct And

Indirect Mechanisms In The Hamster. Reproductive Toxicology, 32, 472-483, 2011 PMID:

21963885

83. Mukherjee A, Reisdorph N, Roy SK. Changes in ovarian protein expression during

primordial follicle formation in the hamster. Mol. Cell. Endocrinol. 348, 87-94, 2012 PMID:

21821096

84. Chakraborty P, Roy SK. Expression of estrogen receptor α36 (ESR36) in the hamster ovary

throughout the estrous cycle: Effects of gonadotropins. PLosOne, 2013, 8 (3)

85. Mukherjee A, Roy SK Expression of ErbB3-binding protein-1 (EBP1) during primordial

follicle formation: Role of estradiol-17β. PLosOne, 2013, 8 (6).

86. Chakraborty P, Roy SK. Expression of FSH receptor in the hamster ovary during perinatal

development. Molecular and Cellular Endocrinology, 400, 41-47, 2014.

87. Chakraborty P, Roy SK. Effect of azaline B on follicular development and functions in the

hamster. Molecular and Cellular Endocrinology, 400, 1-9, 2014.

88. Chakraborty P, Roy SK. Bone morphogenetic protein 2 promotes primordial follicle

formation in the ovary. Scientific Reports (Nature), 2015.

Book Chapters

1. Roy SK, Greenwald GS. Evidence for binding sites for FSH and hCG in mature oocytes of

hamster, mouse, rabbit and rat. In: Proc of the V Ovarian Workshop, DO Toft, RJ Ryan

(eds), p. 143-147, 1984

2. Roy SK, Ogren CL, Roy C. Endocrine and paracrine regulation of ovarian folliculogenesis.

In: Advances in Physiological Sciences, SK Manchanda, et al. (eds), Macmilan India, Ltd,

New Delhi, p. 458-471, 1992

3. Greenwald GS, Roy SK, Wong XN. Isolation and culture of follicles from hamsters, mice

and pigs. In: Methods in Reproductive Toxicology, Academic Press, Inc, Florida, 3B, pp

142-159, 1993

4. Greenwald GS, Roy SK Follicular selection. In: The Physiology of Reproduction, E. Knobil

and JD Neill (eds), Raven Press, New York, pp. 629-724, 1994

5. Roy SK Regulation of follicular development: Beyond gonadotropins. In: Comparative

Endocrinology and Mammalian Reproductive Physiology, KP Jay, C Halder, A Krishna

(eds), Narosha Publishing House, New Delhi, pp. 315-330, 1999

6. Roy SK, Wang C, Mukherjee A, Chakraborty P. In vitro culture of fetal ovaries: A model to

study factors regulating early follicular development. In: Methods in Molecular Biology:



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Germline Development: Methods and protocols, W. Y. Chan and L. A. Blomberg (eds),

Humana Press, New York, vol. 825, 151-171, 2012.

Meeting abstracts: 70

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