1

Curriculum Vitae

Pallab Sarker, PhD Research Assistant Professor

Environmental Studies Program, Dartmouth College, Hanover, NH, USA

Tel.+1(603) 646-6461, Email: pallab.k.sarker@dartmouth.edu

Research Interests

My research interests involve shifting aquaculture, the world’s fastest growing food sector, to sustainability by redesigning the composition of aqua-feeds because they drive life-cycle environmental effects of aquaculture, both inputs and emissions. I also am interested in developing ecological aquaculture principles and practices. The focus of my current research is to develop a fish-free and crop-free aquaculture diet by combining different species of micro- and macro-algae and developing targeted biochemical manipulations to maximize the diet’s nutrient quality, economic viability, and benefits for environmental conservation. Specifically, my major areas of current research are:

• Improve environmental sustainability of aquafeeds while assuring human health benefits of fish raised on micro- and macro-algae diets

• Formulation of environmentally friendly low-pollution (phosphorus and nitrogen loading) feeds

• Evaluation/digestibility/palatability of feed ingredients (marine and freshwater microalgae, microalgae co-products, and terrestrial plant sources)

• Biochemical manipulation of feed ingredients to make environmentally sustainable and economically viable aquaculture feed

• Improvement, standardization, and documentation of methods in fish nutrition applied to advance environmentally sustainable aquaculture.

Education

Ph.D. (Aquaculture) United Graduate School of Agricultural Sciences Ehime University, Japan

2007

M.Sc. (Aquatic chemistry, major: Aquaculture Nutrition) Kochi University, Japan

2004

M.S. (Fisheries Technology, major: Aquaculture Nutrition) Bangladesh Agricultural University, Mymensingh, Bangladesh

1998

B.Sc. (Fisheries) Bangladesh Agricultural University, Mymensingh, Bangladesh

1994

Positions

Research Assistant Professor Nov 2015- Environmental Studies Program, Dartmouth College, Hanover, NH Senior Research Associate in Sustainable Fish Nutrition (Jul 2012~Oct 2015): Environmental Studies Program, Dartmouth College, Hanover, NH Post-doctoral Researcher (Jul 2008~ Jul 2012): Department of Animal Science, Laval University, Quebec, Canada

2

Teaching Assistant and Doctoral Researcher (Oct 2004- Sept 2007): Laboratory of Fish Nutrition, Kochi University, Japan Research Assistant and Master’s Researcher (Oct 2002-Sept 2004): Laboratory of Fish Nutrition, Kochi University Scientific Officer (Aug 1998-Sept 2002): Bangladesh Fisheries Research Institute, Brackishwater Station, Khulna, Bangladesh Master’s Researcher (Jul 1996-Dec 1998): Department of Fisheries Technology, Bangladesh Agricultural University, Bangladesh

Selected Teaching Experience

Ecological Agriculture, ENVS 25 -Dartmouth (undergraduate course; 37 students in the class) Invited Teaching (March 2016): 3rd year BSc level: The Transition from Endogenous to Exogenous

Nutrition in Larval Fish” Faculty of Agriculture, Dalhousie University, Canada. Teaching Assistant (Oct 2004- Sept 2007): Laboratory of Fish Nutrition, Kochi University, Japan, with

Prof. Toshiro Masumoto. Biology Teacher (Nov 1990-Jul 1991): Dutta Higher Secondary School, Netrakona, Bangladesh.

Fellowships/Awards

Post-doctoral research at Laval University with Prof. Grant W. Vandenberg, funded by Natural Sciences

and Engineering Research Council of Canada (June 2009-June 2012). Post-doctoral research at Laval University with Prof. Grant W. Vandenberg, funded by Network in

Aquaculture Quebec (Réseau Aquaculture Québec), Canada (2008-2009). Scholarship (Monbukagakusho) from Government of Japan, Ministry of Education, Culture, Sports,

Science and Technology for Ph.D. study at United Graduate School of Agricultural Sciences, Ehime University (affiliated at Kochi University, 2004-2007).

Scholarship (Monbukagakusho) from Government of Japan Ministry of Education, Culture, Sports,

Science and Technology for M.S. study at Kochi University (2002-2004). Fellowship from Government of Bangladesh for B.Sc. Fisheries (Hons.) and M. S. in Fisheries Technology

(July 1992-December 1998).

Patent

Kapuscinski, A.R and Sarker, P.K., 2016. Aquaculture Feed Formulation and Aquaculture Product Produced with the Same. International application published under the patent corporation treaty. International Publication Number: WO 2016/065231. A1. International patent application number: PCT/US2015/057065.

Successful Funding

3

Sarker, P.K. (lead PI) and A.R. Kapuscinski (co-PI). Development of Cost-Viable and Fishmeal- and Fish Oil-Free Feeds Feeds for Nile Tilapia (Oreochromis niloticus) Using Microalgal Cells and Co-Products. USDA-NIFA: Animal Health and Production and Animal Products. The proposal is awarded for $500,000, from January 1, 2016 to June 14, 2018.

Sarker, P.K., jointly with Grant W. Vandenberg. Review project on Relative efficiency of feeding salmonids:

Sustainability issues related to feeding salmonids: a Canadian perspective)-funded by Aquaculture Collaborative Research and Development Program (ACRDP) and Canadian Aquaculture Industry Alliance (CAIA), Canada (March-September, 2011, CAD 20,000)

Sarker, P.K. Monbukagakusho, Japanese Government fund for M.Sc. and doctoral, 7.9 million yen (US$

100,000). 2002-2007. Sarker, P.K., jointly with M.J. Alam. Development of artificial diet from locally available ingredients for

shrimp, Penaeus monodon. World Bank Project through Ministry of Fisheries and livestocks, Bangladesh. (2002-2003, BDT 100,000 (US$ 1,365)

Grant Proposals Submitted Sarker, P.K. Invention of a fish-free and crop-free aquaculture diet by combining different species of

micro- and macro-algae and developing targeted biochemical manipulations to maximize the diet’s nutrient quality and benefits for environmental conservation. Dartmouth selected me as one of the two nominees for The Moore Inventor Fellows. Proposal submitted on March 5, 2018. Award amount: $675,000 for 3 years, starting from 01/01/19.

Sarker, P.K. (lead PI) and A.R. Kapuscinski (co-PI). Cost-competitive, fish-free and crop-free feeds for Nile

tilapia (Oreochromis niloticus) using microalgae. Pre-Proposal submitted to Foundation for Food Agriculture on April 18, 2018. Total Proposed Budget: 1,000,000 for 3 years, starting from 02/01/19.

Sarker, P.K. (lead PI) and A.R. Kapuscinski (co-PI). Towards Sustainable Fish-free Aquafeed: Enhancing

Performance of Microalgal Co-product in Nile Tilapia (Oreochromis niloticus) Diets. USDA NIFA. Amount requested: $500,000. Proposed duration: 24 months, starting 01/01/18. Declined and positive comments will lead to resubmission.

Kapuscinski A.R., and Sarker, P.K. et al. NSF-INFEWS/T3: Upcycling brewery wastes to produce

microalgae for aquaculture feeds: improving wastewater treatment, increasing energy savings, and reducing pressure on fisheries. Amount requested: $2,318,517. Proposed duration 36 months, starting from 01/01/18. Declined and positive comments will lead to resubmission

Sarker, P.K. (lead PI) and A.R. Kapuscinski (co-PI). Development of Cost-Viable and Fishmeal- and Fish Oil-

Free Feeds for Nile Tilapia (Oreochromis niloticus) Using Effluent-Raised Microalgae (USDA, program area: Animal Health and Production and Animal Products). Submitted May 01, 2014, $500,000. Declined and positive comments led to resubmission and award as mentioned above.

Kapuscinski, A.R. (lead PI) and P.K. Sarker (co-PI). Life-cycle environmental benefits and impacts of the

use of effluent-fertilized microalgae in fish feed in recirculating aquaculture systems. (USDA,

4

program area: Renewable Energy, Natural Resources, and Environment). Submitted on June 01, 2014, $500,000). Declined (plan to revise and resubmit in 2016).

Sarker, P.K. jointly with Grant W. Vandenberg, Laval University, Canada. Bio Cage: A novel nutritional

approach for biological containment of farmed fish (NSERC strategic project, Canada, April 2011, CAD 467,000; 2nd phase with salmonids).

Current Research Projects at Dartmouth College

1. Development of Cost-Viable and Fishmeal- and Fish Oil-Free Feeds Feeds for Nile Tilapia (Oreochromis niloticus) Using Microalgal Cells and Co-Products. (Fund: USDA-NIFA: Animal Health and Production and Animal Products.

• Determine the level of nutrients and anti-nutrients in whole cells and co-product of a promising microalga, Nannochloropsis oculata.

• Determine the digestible/bioavailable nutrient content of this microalgal co-product for tilapia.

• Measure effects of different levels of fishmeal replacement with co-product on the growth and flesh nutritional quality of fish.

• Investigate combining DHA-rich Schizochytrium sp. with Nannochloropsis oculata co-product to maintain fish flesh ω3/ω6 and DHA/EPA ratios that are beneficial for human health when partially or totally replacing fishmeal and fish oil in tilapia diets.

• Compare nutrient digestibility, retention and waste outputs (N and P) of co-product diets to quantify their pollution potential.

• Quantify financial viability of incorporating co-product in tilapia diets.

2. Improving Environmental Sustainability of Feeds for Salmonids Aquaculture: Formulation of Rainbow Trout (Oncorhynchus mykiss) Feed with Complete Substitution of Fishmeal and fish oil using Marine Microalgae (Fund: Sherman Sustainability Science, Dartmouth). Collaborating with Dr. Grant Vandenberg, Université Laval, Canada.

• Digestibility of macronutrients, amino acids and fatty acids in marine microalgae (Nannochloropsis sp. and Isochrysis sp.) feed ingredients for rainbow trout.

• Determine combining whole cells of Isochrysis sp. and Schizochytrium sp. with Nannochloropsis oculata maintain fish flesh ω3/ω6 and DHA/EPA ratios that are beneficial for human health when totally replacing fishmeal and fish oil in trout diets.

3. How does fish oil and fishmeal replacement by different microalgae/co-products affect

phosphorous in aquaculture effluents? (Fund: Sherman Sustainability Science, Dartmouth). 4. Develop an in-vitro method to assess the digestibility of phosphorus and nitrogen compounds

within dried whole cells/co-products of marine water and freshwater microalgae, under chemical conditions that resemble conditions in the digestive system of Nile tilapia (Fund: Sherman Sustainability Science, Dartmouth).

5. Fertilize microalgae with organic waste streams instead of inorganic fertilizers. Life-cycle effects? (Fund: Sherman Sustainability Science, Dartmouth).

5

Projects Conducted at Laval University, Canada (2008-2012)

1. Feed Formulation strategies to improve trout aquaculture sustainability and profitability (Fund: DFO-ACRDP. Co-funded by: SORDAC, FQRNT, RAQ, NRC-IRAP)

• Evaluated various feed ingredients and additives, and developing various economical feed formulae for environmentally friendly and sustainable aquaculture

• Improved feed formulation (nutritional approaches) to reduce phosphorus and nitrogen waste output (effluent) from rainbow trout aquaculture.

• Formulated aquaculture feed to reduce the reliance on marine-derived fishmeal and fish oil with alternative feedstuffs of plant, terrestrial animal or microbial origins.

2. Strategies to prevent off-flavors in fish raised in closed-circuit aquaculture system (NSERC strategic project, Canada)

• Developed strategies to monitor and prevent the occurrence of off-flavor compounds (geosmin and MIB) in RAS (Recirculating Aquaculture System).

3. Bio Cage: A novel nutritional approach for biological containment of farmed fish (NSERC strategic project, Canada)

• Quantified biotin requirement of fish; biotin and avidin interaction in fish; biotin metabolism and physiology and gene expression in fish.

4. Relative efficiency of feeding salmonids: Sustainability issues related to feeding salmonids: a Canadian perspective)-review project funded by ACRDP and CAIA.

• Summarized the recent data regarding efficiency and sustainability of feeding farmed salmonids in Canada, with particular emphasis on Atlantic salmon

Papers in review and in preparation

* denotes Dartmouth undergraduate student or recent alum

1. Sarker, P.K., Kapuscinski, A.R., Nash H.M*., Fitzgerald, D.F., 2018. Towards sustainable Fish-free aquafeeds: Complete substitution of Fishmeal and fish oil with marine microalgae improve growth, nutrient digestibility and Fatty acid deposition in Nile Tilapia (Oreochromis niloticus). Manuscript in preparation.

2. Nash H.M*., Sarker, P.K., Kapuscinski, A.R., Fitzgerald, D.F., 2018. Towards sustainable aquafeeds: Evaluation of phosphorus digestibility, retention, and metabolic phosphorus waste outputs of Nile Tilapia (Oreochromis niloticus) fed microalgae-based fish-free feed. Manuscript in preparation.

3. Sarker, P.K., Kapuscinski, A.R., Proulx, E., Vandenberg, G.W., 2018. Towards sustainable

aquafeeds: Evaluating full substitution of fishmeal and fish oil with three marine microalgae

(Nannochloropsis sp., Isochrysis sp. and Schizochytrium sp.) in diets of juvenile rainbow trout

(Oncorhynchus mykiss). Manuscript in preparation.

6

4. Sarker, P.K., A.R. Kapuscinski, M. Coley*, A. Lanois*, and B.N. Zachary*. 2018d. Digestibility of

energy, protein, amino acids, lipid and fatty acids of Nannochloropsis sp. and Isochrysis sp. by Nile

tilapia (Oreochromis niloticus). Manuscript in preparation.

Peer Reviewed Journal Articles

* denotes Dartmouth undergraduate student or recent alum

Sarker, P.K., Kapuscinski, A.R., Bae, A.Y., Donaldson, E., Sitek, A.J., Edelson, O.F*., Fitzgerald, D.F., 2018. Towards sustainable aquafeeds: Evaluating substitution of fishmeal with lipid-extracted microalgal co-product (Nannochloropsis oculata) in diets of juvenile Nile tilapia (Oreochromis niloticus). PLoS ONE 13(7): e0201315. https://doi.org/10.1371/journal.pone.0201315.

Bélanger-Lamonde, A., Sarker, P.K., Ayotte, P., Bailey, J.L., Bureau, D.P., Chouinard, Y., Dewailly, É.,

Leblanc, A., Weber, J-P., Vandenberg, G.W., 2018. Vegetable oils (combined with soybean, canola and a blend of canola oil and microalgae, Schizochytrium sp) as sustainable fish oil substitutes in rainbow trout diets: An approach to reduce contaminant exposure and feed costs. Journal of Food Quality (under revision R1; manuscript number 7949782).

Shah, M.R., Lutzu, G.A., Asraful, A., Sarker P., Chowdhury, M.A.K., Parsaeimehr, A., Liang, Y., Daroch, M.,

2017. Microalgae in aquafeeds for a sustainable aquaculture industry (review article). Journal of Applied Phycology. DOI 10.1007/s10811-017-1234-z.

Sarker, P.K., Kapuscinski, A.R. Lanois, A.* Livesey, E.* Bernhard, K.* and Coley, M.,* 2016. Towards sustainable aquafeeds: complete substitution of fish oil with marine microalga Schizochytrium sp. improves growth and fatty acid deposition in juvenile Nile tilapia (Oreochromis niloticus). PLOS ONE, http://dx.doi.org/10.1371/journal.pone.0156684.

Sarker, P.K., *Gamble, M.M., *Kelson, S., Kapuscinski, A.R., 2016. Nile tilapia (Oreochromis niloticus) show

high digestibility of lipid and fatty acids from marine Schizochytrium sp. and of protein and essential amino acids from freshwater Spirulina sp. feed ingredients. Aquaculture Nutrition 22: 109-119.

Yossa, R., Sarker, P.K., Proulx, E., Vandenberg, G.W., 2015. Effect of the dietary biotin on zebrafish, Danio

rerio reproduction. Aquaculture Research, 46, 117–130. Yossa, R., Sarker, P.K., Mock, D.M., Lall, S.P., Vandenberg, G.W., 2015. Current knowledge on biotin

nutrition in fish and research perspectives. Reviews in Aquaculture, 7: 59–73. Sarker, P.K., Pilote, A., Auffret, A., Proulx, E., Villemur, R., Vandenberg, G., 2014. Reducing geosmin-

associated off-flavor compounds and metabolic phosphorus waste outputs through dietary phosphorus management in rainbow trout raised in recirculating aquaculture systems. Aquaculture and Environment Interaction, 6: 105–117.

Sarker, P.K., Bureau, D.P., Drew, M., Hua, K., Forster, I., Were, K., Hicks, B., Vandenberg, G.W., 2013.

Sustainability issues related to feeding salmonids: a Canadian perspective. Reviews in Aquaculture, 5: 1-21.

7

Yossa, R., *Sarker, P.K., Mock, D.M., Vandenberg, G.W., 2014. Dietary biotin requirement for growth of

juvenile zebrafish Danio rerio (Hamilton-Buchanan). Aquaculture Research, 45, 1787–1797.

Bai, Z., Pilote, A., Sarker, P.K., Vandenberg, G., Pawliszyn, J., 2013. In-vivo solid phase microextraction with ex-vivo calibration: Determination of off flavor components in live fish. Analytical Chemistry 85: 2328-2332.

Yossa, R., Sarker, P.K., Proulx, E., Saxena, V., Ekker, M., Vandenberg, G.W., 2013. A practical approach for sexing zebrafish Danio rerio. Journal of Applied Aquaculture, 25 (2): 148-153.

Sarker, P.K., Yossa, R., Karanth, S., Ekker, M., and Vandenberg, G.W., 2012. Influences of dietary biotin and avidin on growth, survival, deficiency syndrome and hepatic gene expression of juvenile Nile tilapia Oreochromis niloticus. Fish Physiology and Biochemistry, 38 (4): 1183-1193.

Khan, N., Ashraf, M., Qureshi, N.A., Sarker, P.K., Vandenberg , G.W., Rasool, F., 2012. Effect of similar

feeding regime on growth and body composition of Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) under mono and polyculture. African Journal of Biotechnology, 11(44): 10280-10290.

Sarker, P.K., Fournier, J., Boucher, E., Proulx, E., Noüe de al J., Vandenberg, G.W., 2011. Effects of low

phosphorus ingredient combinations on weight gain, apparent digestibility coefficients, non-fecal phosphorus excretion, phosphorus retention and loading of large rainbow trout (Oncorhynchus mykiss). Animal Feed Science and Technology, 168, 241-249.

Yossa, R., Sarker P.K., Karanth, S., Ekker, M., Vandenberg, G.W., 2011. Effects dietary biotin and avidin on

growth, survival, feed conversion, biotin status and gene expression of zebrafish Danio rerio. Journal of Comparative Biochemistry and Physiology, Part B. 160(4):150-158.

Yossa, R., Sarker P.K., Vandenberg, G.W., 2011. Preliminary evidence of the contribution of the intestinal

microflora to biotin supply in zebrafish Danio rerio (Hamilton-Buchanan). Zebrafish, 8 (4): 221-227.

Vandenberg, G.W., Scott, S.L., Sarker, P.K., Dallaire, V., de la Noüe, J., 2011. Encapsulation of microbial

phytase: effects on phosphorus bioavailability in rainbow trout (Oncorhynchus mykiss). Animal Feed Science and Technology, 169 (3-4): 230-243.

Koko, G.K.D., Sarker, P.K., Proulx, E., Vandenberg, G.W., 2010. Effects of alternating feeding regimes with

varying dietary phosphorus levels on growth, mineralization, phosphorus retention and loading of large rainbow trout (Oncorhynchus mykiss). Aquatic Living Resources, 23, 277-284.

Sarker, P.K., Fukada, H., Masumoto, T., 2009. Phosphorus availability from inorganic phosphorus sources in yellowtail (Seriola quinqueradiata Temminck and Schlegel) Aquaculture 289, 113-117.

8

Sarker, P.K., Shuichi, S., Fukada, H., Masumoto, T. 2009. Effects of dietary phosphorus level on non-faecal phosphorus excretion from yellowtail (Seriola quinqueradiata Temminck and Schlegel) fed purified and practical diets. Aquaculture Research, 40 (2), 225-232.

Sarker, P.K., Hosokawa, H., 2009. Effects of phytase on growth and phosphorus utilization in Japanese flounder (Paralichthys olivaceus). International Journal of Recirculating Aquaculture, 10, 25-41.

Mondal, S., Sarker, P.K., Islam, M.M., Alam, M.J., 2009. Nutritional evaluation of substitute protein

sources in locally made diets for juvenile shrimp, Penaeus monodon. International Journal of BioResearch, 6 (2), 69-73.

Sarker, P.K., Fukada, H., Masumoto, T., 2007. Suitable fecal collection technique for determining apparent phosphorus availability in yellowtail (Seriola quinqueradiata). Aquaculture Science (Japan Aquaculture Society), 54 (3), 319-324.

Sarker, P.K., Fukada, H., Hosokawa, H., Masumoto, T., 2006. Effects of phytase with inorganic phosphorus

supplement diet on nutrient availability of Japanese flounder (Paralichthys olivaceus). Aquaculture Science (Japan Aquaculture Society), 54 (3), 391-398.

Dey, D., Sarker, P.K., Mohiuddin, K.M., Chowdhury, M.A.H., 2006. Litter decomposition and nutrient release pattern in madhupur forest soils of Bangladesh. Bangladesh Journal of Agricultural Sciences, 33 (1), 99 - 109.

Sarker, P.K., Rahman, S., Ahmed, S.U., Rahaman, M., Dey, D., 2005. Suitability and production performance of Oreochromis niloticus (GIFT) and Cyprinus carpio under mono and mixed culture system in coastal rice field. Journal of Biological Sciences, 5 (3), 289-291

Sarker, P.K., Chowdhury, B.B.P., Khan, M.S.A., Pal, H.K., 2002. Mondal S. A study of silver barb (Puntius gonionotus) monoculture Vs. Mixed culture with carps (Cyprinus carpio) in the yard ditches of Bangladesh. Journal of Biological Sciences, 2 (4), 230-231.

Sarker, P.K., Pal, H.K., Rahman, M.M., Rahman, M.M., 2002. Observation on the fecundity and gonado-somatic index of Mystus gulio in Brackishwaters of Bangladesh. Journal of Biological Sciences, 2 (4), 235-237

Ahmed, S.U., Sarker, P.K., Islam, M.N., Kamal, M., Mazid, M.A., 2001. Identification of causes of shrimp quality loss due to farm operation and post-harvesting at depots and markets of Bangladesh. Journal of Biological Sciences, 1 (4), 188 -191.

Sarker, P.K., Rahman, S., Rahman, M.M., Chakraborty, S.C., Islam, M.N., 2000. Protein digestibility of different animal and plant protein sources for Labeo rohita fingerlings. Journal of Biological Sciences, 3 (4), 590- 592.

Chowdhury, B.B.P., Chakraborty, S.C., Sarker, P.K., 2000. Effects of different feeding frequencies on the growth of Indian major carp Labeo rohita (Ham.) fingerlings fed different formulated diets. Progressive Agriculture, 11 (1& 2), 175-179.

9

Sarker, P.K., Chakraborty, S.C., Islam, M.N., Chowdhury, B.B.P., 2000. Effect of animal protein and plant protein diets on growth of Indian major carp (Labeo rohita Ham.) fingerlings. Bangladesh Journal of Fisheries Research, 4 (2), 114- 120.

Rahaman, S. Sarker, P.K., Ahmed, S.U., Rafiquzzaman, M., Ferdous, S.M., 1999. Study on the suitability of

culture of exotic fish species in the coastal paddy fields of Bangladesh under mono and mixed culture system. Journal of Biological Sciences, 3 (4).

Ahmed, S.U., Sarker, P.K., Rhaman, S., Kamal, M., Islam, M.N., Mazid, M.A., 1999. Study on the impact of farm management on the production level of shrimp under various culture system of Bangladesh. Banglsdesh Journal of Fisheries Science, 22 (2) 105-111.

Peer reviewed books, book chapters Sarker, P.K., Masumoto, T., 2009. Nutritional approach for the reduction of phosphorus waste from

yellowtail (Seriola quinqueradiata) aquaculture effluents. In: Aquaculture: Economic and Environmental Impacts (Felice D. C. and Alessio B., eds.) Academic Press/Nova Science Publishers, Inc. Hauppauge, NY 11788. pp. 157-176.

Sarker, P.K., Ahmed, S. U. (2002). Chingrir ahoronttor paricharja abong poribahan prokria: Post-harvest handling and transportation system of shrimp extension booklet in Bengali. Bangladesh Fisheries Research Institute, Paikgacha, Khulna. 13P.

Invited Talks

• Towards sustainable fish-free Aquafeeds: Evaluating microalgal co-product for replacement of fishmeal in aquaculture diets for Nile tilapia (Oreochromis niloticus). World Aquaculture Society conference; Aquaculture America 2018, Las Vegas, USA, February 19- 23, 2018.

• Development of Cost-Viable and Fishmeal- and Fish Oil-Free Feeds for Nile Tilapia (Oreochromis niloticus) Using Microalgal Cells and Co-Products, July 12, 2017, Project Director Meeting, USDA- Agriculture and Food Research Initiative, Baltimore, Maryland, USA.

• Nutrition strategies for improving the environmental sustainability of aquaculture, Dalhousie University, Nova scotia, Canada. March 10, 2016.

• Towards sustainable aquafeeds: Evaluating three marine microalgae for replacement of fish oil and fishmeal in aquaculture diets for Nile tilapia (Oreochromis niloticus) and rainbow trout (Oncorhynchus mykiss). Algae Biomass Summit in Washington, DC, September 29-October 2, 2015. Organized by Algae Biomass Organization.

• Aquaculture Nutrition Strategies for Environmental Sustainability: Environmental Studies Program, Dartmouth College, New Hampshire, USA. February 06, 2014.

• Toward Sustainable Aquaculture to Meet the Food Security Challenge in the World: Hanover Rotary Club, Hanover, New Hampshire, USA. December 11, 2013.

• Highly digestible ingredients for formulating diet for yellowtail (Seriola quinquerdiata): A special lecture for under graduate students, Usa Marine Biological Center, Kochi University, Japan. January 27, 2006

• Consequences of phosphorus pollution from aquaculture: A special lecture for under graduate students at Faculty of Agriculture, Kochi University, Kochi prefecture, Japan. June 21, 2005.

10

• Role of phytase in Japanese flounder (Paralichthys olivaceus) diet: Invited speaker in a summer seminar of Ehime University, Matsuyama, Japan. October 25, 2003

• Present status of production & processing technology of shrimp in coastal region of Bangladesh: Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna, Bangladesh, March 01 2001 (Host: Bangladesh Fisheries Research Institute, Bangladesh).

Conference Presentations and Abstracts

* denotes Dartmouth undergraduate student or recent alum

Sarker, P.K., and A.R. Kapuscinski, O.F. Edelson*, D.S. Fitzgerald 2018. Towards sustainable fish-free Aquafeeds: Evaluating microalgal co-product for replacement of fishmeal in aquaculture diets for Nile tilapia (Oreochromis niloticus). World Aquaculture Society conference; Aquaculture America 2018, Las Vegas, USA, February 19- 23, 2018.

Gao* A., Grimes* C., Sarker P.K., Kapuscinski A.R., Fitzgerald D.S. 2018. Towards Sustainable aquaculture

feeds: Anti-nutrients in competing microalgae and terrestrial crop protein sources. Wetterhahan Science Symposium. Dartmouth.

Schelling* B., Souza*, A., Sarker P.K., Fitzgerald D.S. , Takayuki T., Kapuscinski A. 2018. Towards

Sustainable Aquafeeds: Can enzyme additions improve digestibility? Wetterhahan Science Symposium. Dartmouth

Edelson*, O.F., Bang*, S., Gao*, A., Sarker, P.K., Kapuscinski, A.R., D.S., Fitzgerald. 2017. Making aquaculture feeds less fishy: marine microalgae replacing fishmeal. Wetterhahan Science Symposium. Dartmouth.

Gao*, A., Sarker, P.K., Kapuscinski, A.R., D.S., Fitzgerland. 2017. Determining anti-nutrient content in microalgae-based aquaculture feeds. Wetterhahan Science Symposium. Dartmouth.

Sarker, P.K., and A.R. Kapuscinski. 2016. Evaluation of microalgal co-product as a novel ingredient in Nile Tilapia (Oreochromis niloticus) feed. Poster, 2016 Project Director Meeting, USDA- Agriculture and Food Research Initiative, Salt Lake City, UT, USA.

Sitek, A., McIntyre*, J. Sarker, P.K., and A.R. Kapuscinski. 2015. Sustainable solutions for aquaculture:

Microalgae matters. Poster, 2015 Wetterhahn Undergraduate Research Symposium, Dartmouth. Bernhard*, K.P., Lanois*, A.J., Livesey*, E.D., Kapuscinski, A.R. and P.K. Sarker. 2015. (authors contributed

equally). Sustainable solutions for aquaculture: The power of pH. Poster, 2015 Wetterhahn Undergraduate Research Symposium, Dartmouth.

Sarker, P.K., Bureau, D.P., Drew, M., Hua, K., Forster, I., Hicks, B., Vandenberg, G.W., Were, K., 2011. A

review of sustainability issues related to feeding salmonids: a Canadian perspective. Aquaculture Association of Canada (AAC) 2011, Quebec City, Canada, May 09-11, 2011(Oral).

Sarker P.K., Yossa, N.R., Karanth, S., Ekker, M., Vandenberg, G.W., 2011. Restricted dietary biotin level

induces deficiency syndromes in juvenile tilapia Oreochromis niloticus. World Aquaculture

11

Society: Aquaculture America 2011, New Orleans, Lousiana, USA, February 28-March 3, 2011. (Poster).

Sarker P.K., Yossa, N.R., Karanth, S., Ekker, M., Vandenberg, G.W., 2011. Effects of dietary biotin

restriction on growth, deficiency syndrome and biotin dependent hepatic gene expression of juvenile tilapia Oreochromis niloticus. Association of Canada (AAC) 2011, Quebec City, Canada, May 09-11, 2011 (Poster).

Yossa, N.R., Sarker, P.K., Vandenberg, G.W., 2010. Interactive effect of avidin and biotin on dietary biotin

deficiency in zebrafish, Danio rerio (Hamilton-Buchanan). Annual meeting of the Réseau Aquaculture Québec, RAQ, Levis, Quebec, Canada, September, 23-24.(Poster).

Sarker, P.K., Yossa, N.R., Vandenberg, G.W., 2009. Quantifying the dietary biotin requirement of juvenile

zebrafish, Danio rerio (Hamilton-Buchanan). Annual meeting of the Quebec Forum on marine Sciences (Forum Québécois en Sciences de la Mer) University of Rimouski, Quebec, Canada November, 10-11. (Poster).

Sarker, P.K., Fukada, H., Masumoto, T., 2007. Effects of dietary phosphorus level on non fecal soluble

phosphorus in yellowtail (Seriola quinqueradiata) aquaculture effluents. Annual meeting of the Japanese Society of Fisheries Science. Tokyo University of Marine Science and Technology, Tokyo, Japan. March, 27- 31. (Oral).

Sarker, P.K., Fukada, H., Masumoto, T., 2006. Dietary phosphorus requirement of Japanese flounder

(Paralichthys olivaceus). Annual meeting of the Japanese Society of Fisheries Science. Kochi University, Kochi, Japan. March, 27- 31. (Oral).

Sarker, P.K., Masumoto, T., Hosokawa, H., 2003. Eco friendly diet development and sustainable

aquaculture. The United Graduate School of Agricultural Sciences, Ehime University, September 7- September 9. (Oral).

Sarker, P.K., Takakuwa, F., Masumoto, T., Hosokawa H. 2003. Reduction of inorganic P supplement with

phytase treated Flounder (Paralichthys olivaceus) diet. Annual meeting of the Japanese Society of Fisheries Science. Tokyo University of Marine Science and Technology, Tokyo, Japan. April, 1- 5. (Poster).

Sarker, P.K., Alam, M.J., Islam, M., Mondol, S., 2002. Impact of improved management on shrimp (Penaeus monodon) production and quality. Special workshop on prospect and future of shrimp farming in Bangladesh. April, 25-28. (Oral).

Sarker, P.K., Alam, M.J., Mondol, S., 2001. Environmental conservation and shrimp culture management in coastal region of Bangladesh. National Symposium on Aquaculture Research progress. Bangladesh Agricultural University, Mymensingh March, 8-11. (Oral).

Sarker, P.K., Ahmed, S.U., Pal, H.K., Rahman, S., 2001. Formulation and preparation of shrimp (Penaeus monodon) diet from locally available animal and plant ingredients. Annual meeting of National Agricultural Research and Farming System Research Management. BARC, Bangladesh, September, 5-8. (Oral).

12

Selected Popular Articles (Newspapers/Newsletters/Websites) Kapuscinski A.R. and Sarker P.K. 2014. Study: Tiny Fish Might Hold a Big Answer for Aquaculture. http://now.dartmouth.edu/2013/09/study-tiny-fish-might-hold-big-answer-aquaculture Sarker P.K. (eds.). 2009. BioCage: A novel approach for biological containment of farmed fish. Network in

aquaculture Quebec (Réseau Aquaculture Québec, RAQ), Canada. Annual report 2008-2009, 5p.

Vandenberg, G.W., Ekker, M., Fletcher, G., Létourneau, L., Pothier F., Yossa-Nouga, R., Sarker, P.K., 2009. Biocage to control fish farm escapes through genetically engineered nutritional requirement. Canadian Aquaculture Research and Development Review. Report on Recent Research Project, 42p.

Sarker, P.K., 2001. Shrimp Quality: Maintenance of standard for development. The national daily

newspaper of Bangladesh, The Bangladesh Observer.

Supervision of Graduate and Undergraduate Students/Junior scientists/Assistant: Total 40 At Dartmouth

1. Dartmouth, NH, USA: mentor of 2 Presidential Scholar research students (completed) 2. Dartmouth, NH, USA: mentor of 3 Women in Science Project students (1 completed; 2-ongoing) 3. Dartmouth, NH, USA: mentor of 1 undergraduate senior thesis research student (completed) 4. Dartmouth, NH, USA: mentor of 15 undergraduate summer and fall research assistants

(completed) 5. Dartmouth, NH, USA: mentor of 1 undergrad independent study (ongoing) 6. Dartmouth, NH, USA: mentor of 1 visiting Scholar from Hokkaido University, Japan (ongoing)

At Laval University, Kochi University and BFRI 1. Laval University, Quebec, Canada: mentor of 1 Ph.D. student (2011). 2. Laval University, Quebec, Canada: mentor of 1 master’s student (2011). 3. Laval University, Quebec, Canada: mentor of 1 master’s student (2012). 4. Laval University, Quebec, Canada: 8 summer interns (2008-2011). 5. Kochi University, Japan, Co-supervisor: 2 MS students successfully completed their program

(2005-2007). 6. Bangladesh Fisheries Research Institute, Brackishwater station, Supervise: 3 junior scientists and

4 lab assistants (1998-2002).

Editorial Board and Reviewer for Grant/Peer Reviewed Journals

Grant Peer Review Panel (since 2017): Expert Reviewer for the Grant: Foundation for Food and Agriculture Research

Editorial Board (since 2014)

• Journal of Aquaculture and Marine Biology (http://medcraveonline.com/JAMB/editorial-board )

• EC Nutrition (https://www.ecronicon.com/nutrition-editorial-panel.php )

13

Reviewer

• PLOS One

• Aquaculture Research

• Algal Research

• Comparative Biochemistry and Physiology, part B

• Journal of the World Aquaculture Society

• Journal of Animal Physiology and Animal Nutrition

• Turkish Journal of Fisheries and Aquatic Sciences

• Journal of Shellfish Research

Press Interview

• US: Microalgae co-product may have role in cutting fishmeal use. 2018. Feed Navigator magazine: https://www.feednavigator.com/Article/2018/08/27/US-microalgae-co-product-may-have-role-in-cutting-fishmeal-use

• Sustainable aquafeed: Marine microalga co-product holds potential. 2018. Food Ingredients First magazine: http://www.foodingredientsfirst.com/news/sustainable-aquafeed-marine-microalga-co-product-holds-potential.html#.W337gNVQQLU.linkedin

• A Sustainable Superfood for Farmed Fish. 2016. Conservation Magazine. http://conservationmagazine.org/2016/06/sustainable-substitute-for-fish-oil/

• US Team Raised Value-added Tilapia on Microalgae Diet. 2016. Feed Navigator. http://www.feednavigator.com/Manufacturers/US-team-raised-value-added-tilapia-on-microalgae-diet

Media/Public Outreach Links

Good coverage by science news aggregators in both the environment and feeds arenas: New Atlas (formerly Gizmag) is one of the world's largest independent science and technology publications. Environment: Algae-meal fish food could make aquaculture more eco-friendly https://newatlas.com/tilapia-feed-microalga/55975/

Marine microalga may make aquafeeds more sustainable. 2018. Feedstuffs magazine https://www.feedstuffs.com/nutrition-health/marine-microalga-may-make-aquafeeds-more-sustainable

Scientists develop aquafeed using marine microalga co-product. 2018. Post Online Media: http://www.poandpo.com/agrifish/scientists-develop-aquafeed-using-marine-microalga-coproduct-2082018697

Marine microalgal co-product shows promise as sustainable aquafeed. 2018. Animal Science database: https://www.cabi.org/animalscience/news/65361

14

Making aquafeed more sustainable: Scientists develop feeds using a marine microalga co-product. 2018. Science Daily: https://www.sciencedaily.com/releases/2018/08/180818115645.htm

• https://www.sciencedaily.com/releases/2016/06/160604050639.htm

• https://www.undercurrentnews.com/2016/06/06/ivy-league-college-in-fish-free-tilapia-feed-breakthrough/

• https://www.linkedin.com/pulse/imminent-environmental-threats-major-economic-pallab-sarker?trk=mp-author-card

• https://www.eurekalert.org/pub_releases/2016-06/dc-dtm060216.php

Society Memberships

• World Aquaculture Society

• Aquaculture Association of Canada, Network in aquaculture Quebec (Réseau Aquaculture Québec, RAQ), Canada,

• Agriculturist Institution, Bangladesh (life time),

• Society of Fisheries Science of Japan (2002-2008),

• Japanese Society for Aquaculture Research (2002-2008).

Linguistic skills

-English (fluent): Reading, writing and speaking

-Bengali (fluent): Reading, writing and speaking

-Japanese (fluent): Speaking and writing hiragana and katakana

-French (basic): Speaking

-Hindi (learner): Speaking

Expertise in Techniques

• Fish and shellfish feed formulation and manufacture

• Experimental design, set up, and appropriate advanced statistical analysis (SPSS, SAS) of experimental data

• Bio-chemical analysis of fish sample/tissue and diet

• Analysis of enzyme activities, ELISA, and HPLC

• GC-MS, SPME, ICP, IP, blood and plasma assay and bacterial metabolites (off-flavor compounds)

• Digestibility of ingredients, feed and related chemical analysis, fish effluent characterization

• RNA preparation from tissue, cDNA synthesis, PCR for gene expression study

15

• Fish husbandry (rainbow trout, tilapia, zebrafish, Indian major carp, common carp, silver barb, yellowtail, Japanese flounder, and shrimp)

• Reproduction and breeding of ornamental fish: preparation of embryo media and larval caring

• Flow cytometer for sperm viability, computer assisted semen analyzer (CASA, Ceros Analyzer) for sperm motility

• Haemocytometer for sperm and microalgae density

Extracurricular activities

-Served as captain for Scouts and Guides on School level for three years.

-Served as Student Representative in College for two years

-Served as Workshop/Seminar Coordinator in the Brackishwater Station, Bangladesh Fisheries Research Institute

-Hosted a Japanese television show in a Japanese national TV channel on tourism industry for 2 years (in Japanese language)

-Participated actively in debate, quiz, and sports competition, and cultural programs (notably for singing) at college and University level.