current problems2012.igem.org/files/presentation/nymu-taipei_championship.pdf · achievements we...
TRANSCRIPT
Current problems
Population↑ Pollution↑
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The Solution: Conquering Venus &
COOL!!
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But...
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1. Venus’ atmosphere…
2. Need energy, need food!!
CO2 SO2
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Atmosphere Perform
Long-lasting
CO2
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H2S SO2
How about using H2S as energy source? → Sulfide-Quinone Reductase
Lots of CO2, but no O2 →Photosynthesis →Cyanobacteria
Becoming Venusians → Endosymbiosis, INV-LLO Bba_K299812 iGEM10_Warsaw (2010-10-13)
SO2 as a toxic gas →Sulfite reductase
Practical Application →Purifying polluted air
Overcoming difficulties
How about using H2S as energy source? → Sulfide-Quinone Reductase
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Synechocystis PCC7942
Sulfite reductase
SO2
Sulfide quinone
reductase (sqr)
chloroplast +
S + (CH2O)n
C6H12O6
For Host Cell to
Use
Whole picture of our project: Inside of the Stem Cell
Glut
CO2
H2S
SO2
CO2
H2S
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Nucleus
Synechocystis PCC7942
How to become Venusians?
Desulfovibrio desulfuricans
Synechococcus sp. PCC 7002
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Engineered Synechocystis PCC7942
How to become Venusians?
Glut (BBa M11036 )
Invasin-listeriolysin O
(BBa_K299812)
Into the
Stem cell 7 NYMU-Taipei
CO2
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H2S SO2 SO2 as a toxic gas →Sulfite reductase
Sulfur Dioxide (SO2) Reducer
Solved!!
Synechocystis
PCC7942
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Sulfur Dioxide (SO2) Reducer: The Concept
Desulfovibrio desulfuricans Pseudomonas aeruginosa
Dsr Sulfite reductase CysI Sulfite reductase
SO2 HSO3- H2S
dissolve in water Sulfite reductase
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H2S serial dilution
and standard curve detection
When H2S exist,
DPDA solution turn
turbid.
H2S producing assay
Lavilla I,Pena-Pereira .F et al, Analytica Chimica Acta 647 (2009) 112–116
Sulfur Dioxide (SO2) Reducer: The Experiment
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WORK Sulfur Dioxide (SO2) Reducer: The Results
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Experiment OK!! More H2S!!
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H2S SO2
Using H2S to generate energy How about using H2S as energy source? → Sulfide-Quinone Reductase
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
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Sulfite reductase Sulfide quinone
reductase(sqr)
chloroplast
Synechocystis PCC7942
H2S
SO2
+
e-
H2S: Novel Energy Currency
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S + (CH2O)n
CO2
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Sulfur Version of
Photosynthesis H2S S+2H++energy
SQR
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Using H2S to generate energy: The Concept
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2H2O O2 +4H++energy
Cyanobacteria
DCMU challenged assay
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Using H2S to generate energy: The Results
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sqr has
advantage
DCMU Cyanobacteria growth
Sodium Sulfide consumption in E. coli SQR grows better!
Flow cytometer – Exact cell number Auto-fluorescence assay method (2011
Schulze )
sqr strain has
larger cell number
and stronger
fluorescence signal
Plate Count
sqr strain
Wild type
sqr strain has more colonies
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Using H2S to generate energy: The Results
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Sodium Sulfide
consumption in E. coli
Sodium Sulfide leftovers
after 48 hours
2.2 times!
sqr strain consumed
most of the sodium
sulfide WORK
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H2S SO2
Apoptosis
Creating Venusians
Becoming Venusians → Endosymbiosis
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
Solved!! sqr
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Invasin(inv) from Yersinia pestis
HEp-2 cell
Inv+
E.coli
listeriolysin O(llo) from
Listeria monocytogenes
HeLa cell
llo+ E.coli
[1]Isberg, R.R., and Falkow, S. (1985) A single genetic locus encoded by Yersinia pseudotuberculosis permits invasion of
cultured animal cells by Escherichia coli K-12. Nature 317: 262-264. [2]Denise M. Monack,Julie A. Theriot(2001).” Actin-based motility is sufficient for bacterial membrane protrusion formation and
host cell uptake”. Cellular Microbiology (2001) 3(9), 633-647
Endosymbiosis between Eukaryotes and
Cyanobacteria(inv-llo +)
Agapakis CM, Niederholtmeyer H, Noche RR, Lieberman TD, Megason SG, Way JC, & Silver PA (2011).
Towards a synthetic chloroplast. PloS one, 6 (4) PMID: 21533097
CHO cells J774 macrophages
cells Invasin(inv) from Yersinia pestis
listeriolysin O(llo) from Listeria
monocytogenes
Cells:
• J774 macrophage cells line
• Induced pluripotent stem cells
SO2 H2S S CysI SQR
Our work
J774 macrophage cell line
with cyanobacteria invasion
Induced pluripotent stem cells(iPS cells)
with cyanobacteria invasion
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H2S SO2
Practical Applications To confirm our project
Practical Application →purifying polluted air →confirming our project practically
SO2 CO2
Practical Experiments to confirm: The Experiment Chung Hwa Pulp Corporation
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Co-culture:
Engineered bacteria+
industrial waste
For waste gas cleaning
Water layer Gas layer
Practical Applications: The Experiment
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WORK
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H2S producing assay
For waste water cleaning
Water layer Gas layer
Practical Applications: The Experiment
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WORK
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H2S producing assay
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H2S SO2
Completing whole parts!!
Solved!!
Synechocystis
PCC7942
Solved!!
Sulfite reductase
Solved!! sqr
Solved!!
INV-LLO
Synechocystis
PCC7942
Work!!
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Integrating all factors!!
Solving all problems!!
Let’s go to VENUS!!
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Human Practice—what have they done Brand New Idea Communicating with aliens by Arecibo code Meeting with other Taiwan iGEM teams
6/1 Meeting with NTU-Taida
8/15 Taiwan iGEMer Fest (Press Release)
Having Fun
Going to Hot Spring Valley
Visiting Taipei Astronomical Museum
Advocating our project Board Game
iGEM song
iGEM short film
Advocating iGEM & synthetic biology
7/27 Synthetic Biology Worldwide
8/26 Welcome Party for Freshmen
9/13 Exhibition of iGEM in NYMU
Arecibo code scattering around Taipei
6/1 Meeting with NTU-Taida
8/15 Taiwan iGEMer Fest (Press Release)
Their E.coli
works
Creating a whole new method to exchange and
discuss ideas within Taiwan iGEM teams for themselves
and the future 2013、2014、2015… Taiwan iGEMers
Let people join iGEM in the future easier
Advocating 2012 NYMU
iGEM team
B o a r d G a m e i G E M s o n g
i G E M s h o r t f i l m
Advocating iGEM &
synthetic biology
7/27 Synthetic Biology Worldwide 8/26 Welcome Party for Freshmen
9/13 Exhibition of iGEM in NYMU
To attract more people to
What do those mean ?
To let more people to what is iGEM & synthetic biology
know
To make people iGEM &synthetic biology interested in
To let some of those people who start to realize iGEM &synthetic biology to us!! join
engage in synthetic biology field!
Achievements We have submitted 11 parts to part registry.
We have characterized and proved that these new biobrick parts:
SQR, CysI, DsrI really work as our expectation.
We have modified BBa_K299812 (INV+LLO) and elevated our
idea to a higher level, to create venusian!
We have held a meet-up with Taiwan iGEMers, with board
games, iGEM songs, movies and coorperated with NTU-
Taida.
We have already outlined and detailed an extremely new human
practice - outer space human practice project (New human
practice approaches!!).
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We are the first iGEM team who tries to…
communicate with the aliens!!
We had lots of… FUN & FUN &FUN!!
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Reference
P1 http://www.google.com.tw/imgres?hl=zhTW&sa=X&tbm=isch&prmd=imvns&tbnid=qOU_cLnW5mjJKM:&imgrefurl=http://www.geekosystem.com/ben-stiller avataroscars/&docid=cztpwj_qRRdhBM&imgurl=http://www.geekosystem.com/wpcontent/uploads/2010/03/benstilleravatar.jpg&w=461&h=285&ei=97tnUOmbGM7umAX8hoCIAw&zoom=1&iact=rc&dur=375&sig=109169273201630242688&page=2&tbnh=139&tbnw=197&start=18&ndsp=22&ved=1t:429,r:12,s:18,i:167&tx=144&ty=61&biw=1280&bih=642
P5
computer fixerhttp://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=h2Q2KTB_ECeQFM:&imgrefurl=http://www.techsonsite.co.uk/&docid=REnxlPXOqynqOM&imgurl=http://www.techsonsite.co.uk/wp-content/uploads/2011/03/milton-keynes-computerrepairs.png&w=359&h=268&ei=Vb1nUNaWO4XLmgWCk4HICg&zoom=1&iact=hc&vpx=977&vpy=205&dur=18&hovh=194&hovw=260&tx=197&ty=124&sig=109169273201630242688&page=1&tbnh=142&tbnw=192&start=0&ndsp=18&ved=1t:429,r:11,s:0,i:101&biw=1280&bih=642
lightbulb
http://www.google.com.tw/imgres?hl=zhTW&tbm=isch&tbnid=kfPkZWWGCXwYbM:&imgrefurl=http://blog.enn.com/%3Fp%3D481&docid=MdxonwH6otD7aM&imgurl=http://blog.enn.com/wp-content/uploads/2010/10/lightbulb.jpg&w=315&h=387&ei=gL1nULjHFMXLmAX-8YGQDQ&zoom=1&iact=rc&dur=143&sig=109169273201630242688&page=2&tbnh=142&tbnw=113&start=23&ndsp=31&ved=1t:429,r:6,s:23,i:162&tx=64&ty=89&biw=1280&bih=642
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http://www.google.com.tw/imgres?hl=zh-
TW&tbm=isch&tbnid=cgZK8mOqlDXOaM:&imgrefurl=http://xiaosinyi.pixnet.net/blog/post/33570223motivation!&docid=UrV5bFu-KDNZVM&imgurl=http://4.bp.blogspot.com/_ccEezkECpdg/TSSQQ_jaMHI/AAAAAAAADsA/H1sMe6pdUKA/s1600/motivation.jpg&w=900&h=900&ei=mb1nUPScF4_JmQXQ94CwCg&zoom=1&iact=rc&dur=1&sig=109169273201630242688&page=2&tbnh=139&tbnw=139&start=17&ndsp=24&ved=1t:429,r:19,s:17,i:186&tx=69&ty=83&biw=1280&bih=642
p7
relay race
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=98OqvAVMjU7UfM:&imgrefurl=http://thepauls.wordpress.com/2011/01/31/the-gospel-baton/&docid=2trO2aDES7n-fM&imgurl=http://thepauls.files.wordpress.com/2011/01/pass_the_baton.jpg&w=1024&h=768&ei=y71nUJTJAe_LmAWiiIDQCg&zoom=1&iact=rc&dur=137&sig=109169273201630242688&page=1&tbnh=134&tbnw=230&start=0&ndsp=16&ved=1t:429,r:3,s:0,i:76&tx=110&ty=74&biw=1280&bih=642
p8
book
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=VUrSo60jjoGlSM:&imgrefurl=http://www.armenianweekly.com/2012/04/06/the-armenians-and-the-book-exhibit-to-open-at-harvard/&docid=DNtmCdMCXvPmIM&imgurl=http://www.armenianweekly.com/wp-content/uploads/2012/04/book.jpg&w=600&h=450&ei=7L1nUO-oC6POmgWu-4GoBg&zoom=1&iact=rc&dur=279&sig=109169273201630242688&page=2&tbnh=139&tbnw=188&start=18&ndsp=24&ved=1t:429,r:5,s:18,i:144&tx=83&ty=53&biw=1280&bih=642
p9
shortcut
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=p3FTTaefkBrCuM:&imgrefurl=http://lostinexcel.blogspot.com/2012/02/all-worksheet-shortcuts-one-place.html&docid=_Zhh_o5KDcQe6M&imgurl=http://4.bp.blogspot.com/-0nKLyRIQYME/T0JGf7ScQpI/AAAAAAAAEHA/rnRE-MtXIFk/s1600/worksheet%252Bshortcut%252Bkeys.jpg&w=517&h=342&ei=EL5nUPzIB6WNmQXno4CgBg&zoom=1&iact=hc&vpx=748&vpy=173&dur=96&hovh=182&hovw=276&tx=121&ty=108&sig=109169273201630242688&page=4&tbnh=128&tbnw=193&start=69&ndsp=24&ved=1t:429,r:9,s:69,i:320&biw=1280&bih=642
p10
Arecibo code
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http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=udPyJF8_tSyg5M:&imgrefurl=http://pzzled.yolasite.com/and-everything-in-between.php&docid=KpEVCsQ7jEh26M&imgurl=http://pzzled.yolasite.com/resources/eg-arcibo-glyph.jpg%253Ftid_1258886625188&w=462&h=460&ei=S75nUML-GYGKmQXuwIGQAQ&zoom=1&iact=rc&dur=224&sig=109169273201630242688&page=1&tbnh=131&tbnw=132&start=0&ndsp=23&ved=1t:429,r:2,s:0,i:73&tx=94&ty=70&biw=1280&bih=642
p18
surprise
http://www.google.com.tw/imgres?hl=zh-TW&sa=X&tbm=isch&prmd=imvns&tbnid=HvNqWchw5GJZuM:&imgrefurl=http://dealcetera.com/sears-canada-72-hour-surprise-sale-october-8-10th-savings-and-discounts-up-to-50-off-searsca/&docid=7C58RkoEAUraJM&imgurl=http://dealcetera.com/wp-content/uploads/2009/10/surprise.jpg&w=267&h=400&ei=yb5nUOX-PM7UmAWq4IG4BQ&zoom=1&iact=rc&dur=250&sig=109169273201630242688&page=2&tbnh=141&tbnw=94&start=18&ndsp=26&ved=1t:429,r:7,s:18,i:151&tx=55&ty=54&biw=1280&bih=642
cat ball
http://www.google.com.tw/imgres?hl=zh-TW&tbm=isch&tbnid=xE69VYj-jkZ-oM:&imgrefurl=http://fineartamerica.com/featured/6891-pet-impressions-cat-with-ball-kyle-adamache.html&docid=KSw-QmydUtAoIM&imgurl=http://images.fineartamerica.com/images-medium/6891-pet-impressions-cat-with-ball-kyle-adamache.jpg&w=525&h=700&ei=6L5nUP-QNozMmAWJu4AY&zoom=1&iact=rc&dur=241&sig=109169273201630242688&page=2&tbnh=144&tbnw=117&start=18&ndsp=24&ved=1t:429,r:5,s:18,i:144&tx=73&ty=98&biw=1280&bih=642
join us
http://www.google.com.tw/imgres?start=163&hl=zh-TW&tbm=isch&tbnid=vEmiKOcs244jwM:&imgrefurl=http://km2.iiietc.ncu.edu.tw/xms/content/show.php%3Fid%3D29907&docid=y9V1AVfLMAvYFM&imgurl=http://km2.iiietc.ncu.edu.tw/xms/data/256/content/86E6DD72A5A058C0DB5D/attach/C1000086JoinUs.jpg&w=600&h=848&ei=Er9nUJuuCerKmgWSvoHYCQ&zoom=1&iact=hc&vpx=472&vpy=278&dur=1437&hovh=267&hovw=189&tx=119&ty=183&sig=109169273201630242688&page=8&tbnh=133&tbnw=94&ndsp=25&ved=1t:429,r:2,s:163,i:243&biw=1280&bih=642
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