Culture mediaISO 11290

Culture Media in ISO 11290

Biochemical Test

Listeria Half Fraser Broth Base Cat. 1183

Listeria Fraser Broth Base Cat. 1182

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

MotilityHemolysis Carbohydrate utilization

Listeria

Culture Media in ISO 11290

Biochemical Test

Listeria Half Fraser Broth Base Cat. 1183

Listeria Fraser Broth Base Cat. 1182

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

Esculin is hydrolized to

esculetin, that reacts with ferric salts

Allows the growth of Listeria with a black color

, inhibiting the acompanying flora

Incubate at 30ºC during24 ± 2 hours

Peptones / Salts / BufferLithium Chloride/

Nalidixic AcidEsculin/Ferric Amonium

Citrate

Listeria Half Fraser Broth

Nalidixic Acid inhibits Gram

negative microorganism

sLithium Chloride inhibits Enterococcus that can hydrolize the Esculin

Culture Media in ISO 11290

Biochemical Test

Listeria Half Fraser Broth Base Cat. 1183

Listeria Fraser Broth Base Cat. 1182

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

Peptones / Salts / BufferLithium Chloride/

Nalidixic AcidEsculin/Ferric Amonium

Citrate

Listeria Fraser Broth Base

Allows the growth of Listeria with a

black color , inhibiting the

acompanying flora

Incubate at 35-37ºC during

24-48 hours

Nalidixic Acid inhibits Gram

negative microorganisms

Esculin is hydrolized to esculetin, that reacts with ferric

salts Lithium Chloride inhibits

Enterococcus that can hydrolize the Esculin

Culture Media in ISO 11290

Listeria Half Fraser Broth Base Cat. 1183

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

Peptones / Salts / BufferLithium Chloride/

Esculin/Ferric Amonium Citrate/Other antibiotics

Listeria Agar Base Oxford

Incubate at 32±2°C during 18-24 hours

Listeria monocytogenes and

Listeria innocua grow giving the medium a

black color

Esculin is hydrolized to esculetin, that

reacts with ferric salts

Listeria Fraser Broth Base Cat. 1182

Biochemical Test Cicloheximide

inhibits yeast and molds

Lithium Chloride inhibits Enterococcus that can hydrolize

the Esculin

Culture Media in ISO 11290

Biochemical Test

Buffered Peptone water Cat. 1402

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

Peptones / Salts / Chromogenic Substrate

Lithium Chloride/ Antibiotics

Listeria Chromogenic Agar Base

Incubate at 37ºC during after 24 ± 2 hours.Incubate the negative

strains for 24±2 hours more

Salmonella growth in magenta colonies

Esterase activity cleaves one of the cromogenic substrate, giving

the colony a magenta color

β-Galactosidase activity cleaves

the other chromogenic

substrate, giving the colony a green color

Listeria Fraser Broth Base Cat. 1182

Culture Media in ISO 11290

Biochemical Test

Buffered Peptone water Cat. 1402

Listeria Agar Base Oxford Cat. 1133 & Listeria

Chromogenic Agar Base Cat. 1345

MotilityListeria Fraser Broth

Base Cat. 1182

Hemolysis

Carbohydrate Utilization

Results