csii-cmv-rfa · csii-cmv-rfa 10265 bp mlui 228 nhei 3165 eco47iii 3168 agei 3174 xhoi 3180 noti...
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CSII-CMV-RfA
10265 bp
MluI 228
NheI 3165Eco47III 3168AgeI 3174XhoI 3180
NotI 3318BamHI 3389
EcoRI 3639MluI 4086BamHI 4092
PstI 4761
PstI 4905NotI 4910
BamHI 4918XbaI 4924
HpaI 4930
ApaI 6247
CMV RU55' SD
RRE
3' SAcPPT+CTS
CMV
RfA
attR1
Cm
ccdBattR2PREdel U3RU5
BGH pAf1 ori
SV40 pro & ori
Zeo
SV40 pA
ColE1 ori
Amp 1
Miyoshi
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Plasmid name: CSII-CMV-RfA Plasmid size: 10262 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence RfA: Gateway Reading Frame Cassette A containing Cm, ccdB, attR1, and attR2 Cm: Chloramphenicol resistance gene ccdB: The ccdB gene encodes a protein that interferes with E. coli DNA gyrase attR1, 2: Sites for site-specific recombination with a Gateway vector (Invitrogen). attR sites always recombine with attL sites in a reaction mediated by the LR Clonase (Invitrogen). PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene *Use the DB3.1 or ccdB Survival 2 T1R E. coli strain for propagation of plasmids containing the ccdB gene.
Miyoshi
テキストボックス
y