cs (soft)
DESCRIPTION
Puroindolines ‘ a ’ and ‘ b ’ are completely linked with hardness. Puroindoline a. Puroindoline b. Absence of this gene is always associated to hardness. Presence of the PCR band for the “soft” allele combined with presence of the puroindolin a is characteristic of a soft wheats. - PowerPoint PPT PresentationTRANSCRIPT
CS
(S
oft
)
RS
I5 (
Ha
rd)
An
za (
Ha
rd)
Yec
ora
Ro
jo (
Ha
rd)
UC
10
41
(Ha
rd)
Exp
res
s (H
ard
)
UC
10
36
(Ha
rd)
UC
10
37
(Ha
rd)
PuroindolinePuroindoline aa
• Absence of this gene is always associated to hardness
• Absence is always associated with a puroindoline bb mutation present in soft wheats
RFLP Puroindoline aa
CS
(S
oft
)
UC
10
36
(Ha
rd)
Yec
ora
Ro
jo (
Ha
rd)
Ch
ey
en
ne
(H
ard
)
Exp
res
s (H
ard
)
BC
1 E
xpre
ss /
2*U
C10
36
PCR of puroindoline bb sequence for the “soft” allele (Giroux & Morris TAG, 1997)
Puroindoline b
•Presence of the PCR band for the “soft” allele combined with presence of the puroindolin aa is characteristic of a soft wheats
HaeHae III- MTA9III- MTA9
BC
1 E
xpre
ss /
2*U
C10
36
BC
1 E
xpre
ss /
2*U
C10
36
BC
1 E
xpre
ss /
2*U
C10
36
Puroindolines ‘aa’ and ‘bb’ are completely linked with hardness