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“IntelliBond Trace Minerals”Shattering Trace Mineral Perceptions!

Micronutrients

• Second largest manufacturer and supplier of trace mineral nutrition in NA

• Dedicated only to trace mineral nutrition in livestock• Third party auditors certify high product quality

– HAACP, ISO 9002, Safe Feed, Safe Food, FAMIQs

• All products American made in Indianapolis, In

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IntelliBond Trace Minerals:

• Fully defined crystalline structures– High trace metal potency – Non-hygroscopic – Not soluble above pH 4.0

• Complete rumen by-pass

• Increased trace metal absorption• Greater stability of essential nutrients (vitamins, etc.)

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Trace Mineral Nutrition in Ruminants

• Sulfates / Oxides used from the 50’s– Highly reactive in feeds and the rumen– Lower cost per unit of metal

• Organics developed in the 1970’s and 80’s– Improved stability in feeds

– Better trace mineral absorption

– High cost per unit of metal

• Hydroxy trace minerals (IntelliBond®)– No adverse impact on essential nutrients in feeds– Complete rumen bypass of essential metals – Increased trace metal absorption in the small intestine– Reduced cost per unit of metal

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Smart Release Crystals - Protect Essential Nutrients Present in Premixes & Feeds

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Storage time (d)0 10 20 30 40

Fee

d V

itam

in E

(m

g/kg

)

0

10

20

30

40

50

60

70 Control (18 ppm)Cu sulfate (200 ppm)IntelliBond C (200 ppm)

*

*

* *

* Within a time point, mean is significantly different from Control (p<0.05).Data adapted from Lu et al., 2010. Biol. Trace Elem. Res. 138:181-9.

Feed levels correspond to lower liver and plasma Vit. E in CuSO4

fed chicks (p<0.05).

Mash, Broiler diet with 3.6% added fat

32% loss in Vitamin E activity

at 10 days

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Impact of Vitamin E Degradation

Assumptions:

• Cow receiving 750 IU Vit. E per day in a 3.0 lb supplement, that also delivers -

• 15 ppm copper 75 ppm Zinc and 45 ppm manganese in final ration

• Supplement specs = 0.055% Vit E, 243 ppm Cu, 1,213 ppm Zn & 728 ppm Mn

• Premix sustains a 20% loss in its Vit. E content within two week storage period

Results:

• At 750 IUs per day, cow consumes .55 Kgs per year, E 50% ($9.30 Cow/yr)

• 750 IUs / 50% = 1,500 mg/d E 50% X 365 days = .548 Kgs / yr x $17.00 Kg)

• 20% loss = .11 Kg Vit. E 50% resulting in a loss of $1.86 / cow in Vit. E activity)

Pay back: (Greater than 4.0:1)

• Cost of 15 ppm Cu from CuSO4 = .48 Kgs ($1.35) cow per year

• Cost of 15 ppm from IntelliBond C = .21 Kgs ($1.80) cow per year

• Incremental cost to replace copper sulfate is $.45 cow per year

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CuH2O

OH2

OH2

H2O

O

SO

O

O

Typical Organic Trace Mineral

X

Smart Release Crystals - Support Rumen / Abomasum By-Pass

Inorganic trace mineral

IntelliBond CDefined Crystal Matrix

CuHO

OH

OH

HO

OH

Cl

CuHO

OH

OH

HO

Cl

Cl

Stretched Covalent Bond

Covalent BondAt a pH of < 3.5

Soluble form

Trial Design:•98 day depletion period•60 steers •3 X 2 factorial setup•10 steers / treatment•Trts @ 0, 5 & 10 ppm Cu•5 ppm Mo & .15 % S

J.W. Spears et al. N.C. State University

IntelliBond C Relative Bioavailability In Cattle

IntelliBond Z Relative Bioavailability In Cattle

Smart Release Crystals –Increase Trace Mineral Intestinal Absorption

Dependent Variable Zinc Source

RBV P=

Absorbed Zn ZnSO4

IBz

100

205

0.01

Retained Zn ZnSO4

IBz

100

204

0.01

Dependent Variable Copper source

RBV P=

Plasma Cu CuSO4

IBc

100132

0.07

Liver Cu CuSO4

IBc

100196

0.04

J.W. Spears et al. N.C. State University

Trial Design:•16 yearling steers•Depleted 14 days (basal @ 25 ppm Zn)•Treatments: Zinc sulfate @ 25 ppm IntelliBond Z @ 25 ppm•Seven day adaptation•Five day total collection Urine & fecal output Feed in & Orts

2 X 2 X Increase Increase in RBVin RBV

IntelliBond C vs. OTM In Broilers:

Added Copper Levels (ppm)

Test Materials Starter Grower Finisher

Control (no added Cu) None None None

TBCC 25 25 25

TBCC 50 50 50

TBCC 75 75 75

TBCC 100 100 100

TBCC 125 125 125

TBCC 150 150 150

TBCC 175 175 175

Cu Metal Proteinate 25 25 25

Cu Metal Proteinate 50 50 50

Cu Metal Proteinate 75 75 75

Cu Metal Proteinate 100 100 100

Cu Amino Acid Complex 25 25 25

Cu Amino Acid Complex 50 50 50

Cu Amino Acid Complex 75 75 75

Cu Amino Acid Complex 100 100 100

• Ross birds grown to 56 days of age

• Floor pens, w/ used litter (.8 ft2 / bird)

• 10 replicates of each treatment (25 birds per pen) Results:•All sources improved F/G

•No significant difference at any point

A.H. Pharma Research Institute

IntelliBond C vs. Cu Lysine in Lambs

Study Design:•50 wether lambs w/ initial BW of 23.8 Kg •10 lambs / trt, individually housed in wood pens

•All lambs on common diet first 3 weeks•At week 4, lambs weighed & blocked into 5 trts•60 day evaluation

• Orts were recorded and feed intake adj’d weekly

102011. Biol. Trace Elements Res. Vol 144. pp 570 - 579.

• Treatments:– Control: – no supplemental copper– Trt 2: 10 ppm Cu from Cu Lysine– Trt 3: 20 ppm Cu from Cu Lysine

– Trt 4: 10 ppm Cu from IntelliBond C– Trt 5: 20 ppm Cu from IntelliBond C

• Measurements:

– Blood drawn at days 0, 30 and 60– All lambs harvested at 60 days, livers

collected and assayed

Treatments Contrast P Value

Item Control

Cu Lysine 10 ppm

Cu Lysine 20 ppm

IB C 10 ppm

IB C 20 ppm SEM

ContVsCu

10 vs 20

ppm

IB C vs Lys

10

IB C vs Lys

20

Plasma Cu (mg/l)

Day 0 0.74 0.84 0.78 0.77 0.81 0.02 0.29 0.82 0.35 0.67

Day 60 0.89 0.96 1.08 1.03 1.09 0.03 0.02 0.11 0.32 0.89

Liver Cu (mg/Kg) 45.4 278.5 373.8 297.0 405.1 29.8 0.01 0.01 0.47 0.23

IntelliBond C and Cu-lysine were of similar availability when fed to finishing lambs.

IntelliBond C vs. Availa Cu in Yearlings

Study Design:•24 Brahman crossbred steers

•72 day study•4 Trt / 6 pens per•Trt’s

– 10 ppm Cu – Availa Cu– 10 ppm Cu – IntelliBond C– 15 ppm Cu Availa Cu + 15 ppm Cu IntelliBond C– 30 ppm Cu IntelliBond C

•Free-choice access to star-grass hay•Fed molasses supplement with Trts•Measurements – Liver copper at:

– 0, 24, 48 & 72 days

DayIBC

10 ppmAvaila C10 ppm

IBC30 ppm

IBC/ Availa C30 ppm

0 146 161 125 158

24 136 151 182 163

48 143 145 195 151

72 166 157 226 213

*Liver Cu expressed as PPM Dry MatterJ. Anim. Sci. 2007 85:871-876J. Arthington, Ph.D / J. Spears, Ph.D.

IntelliBond C and Availa Cu were of similar availability when fed to

yearling steers

All cattle on 10 ppm Cu Availa Cu

IntelliBond® Value PropositionRuminants

100% Sulfates 2 (many known antagonists)

100% Hydroxy Trace Minerals 2 (IntelliBond®)

75% Sulfates 2 + 25% OTM 2

100% Organic Minerals 2 (OTM)

Mineral Cost1

(Cow / Year)

$5.16

80% Hydroxy Trace Minerals

$37.40

$13.22

$11.77

$9.42

==

1) Mineral cost for a lactating dairy cow consuming 22 Kgs DM / day2) Supplementation levels

1) Zinc @ 80 ppm2) Manganese @ 40 ppm3) Copper @15 ppm

Cornell – Transition Cow Study Design

Design:• Transition cow study (21 days prepartum through 84 days post partum)

• 60 total cows – 3 treatments of 20 cows each– Cows individually fed (tie stall)

• Cows milked 2x / day, within the Cornell milking facility• Milk weighed at each milking• Separate composite milk sample taken from AM / PM milkings weekly

Treatments: (zinc @ 60 ppm; Mn @ 40 ppm & Cu @ 15 ppm)

– All sulfate– 75% sulfate / 25% Availa Zn, Mn & Cu– All IntelliBond (hydroxy trace minerals)

Measurements:• All performance parameters

• Cow well being & health13 T. Overton, Ph.D, Cornell University, Ithaca, NY

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Cornell Transition: Fat Corrected Milk

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Cornell Transition: Performance Results

T. Overton, Ph.D, Cornell University, Ithaca, NY

Parameter Inorganic ITM / Availa IntelliBond SEM Trt. Week Trt. / Week

DMI (Kg/d)

PrepartumPostpartum

15.025.4

14.824.5

14.825.8

.03

.05NSNS

<.0001<.0001

NSNS

Milk (Kg/d) 45.3 45.7 46.6 1.1 NS <.0001 0.019

Fat (Kg/d) 1.48 1.45 1.49 .05 NS <.0001 0.109

Protein (Kg/d) 1.34 1.33 1.37 .04 NS 0.002 NS

Lactose (Kg/d) 2.14 2.16 2.18 .05 NS <.0001 0.053

FCM (Kg/d) 43.6 43.3 44.3 1.15 NS <.0001 0.027

No statistical differences in Gait Scores, SCC, BCS or other health parameters

Availa® - Registered Trademark of Zinpro Corporation

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Cornell Transition: Oxidative Stress

Treatments P-value

Item ITM ITM/OTM IntelliBond SEM Trt Wk Trt x Wk

TBARS, uM (Thiobarbituric acid-reactive substances)

Whole study 2.11A 1.98AB 1.95B 0.07 0.16 <.01 0.40

Prepartum 1.47 1.49 1.38 0.06 0.37 0.16 0.74

Postpartum 2.26 2.11 2.10 0.09 0.29 <.01 0.23

TAC, mM (Total antioxidant capacity)

Whole study 2.14a 2.07ab 1.93b 0.07 0.09 <.01 0.94

Prepartum 2.09a 1.93ab 1.84b 0.08 0.10 0.01 0.86

Postpartum 2.16a 2.09ab 1.95b 0.08 0.12 <.01 0.88 A,B Least square means within row with different upper-case superscript tend to differ (P < 0.1)

a,b Least square means within row with different lower-case superscript differ (P < 0.05)

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Parameter InorganicLbs (value)

Combo (ITM/OTM)

Lbs (value)

IntelliBondLbs (value)

IntelliBond Incremental Value ITM vs. IB Combo

vs. IB

Milk (lbs) 99.9 ($19.17) 100.8 ($19.34) 102.7 ($19.71) $.54 cow/d $.37 cow/d

Fat (lbs) 3.26 ($5.68) 3.20 ($5.57) 3.28 ($5.71) $.03 cow/d $.14 cow/d

Protein (lbs) 2.95 ($9.78) 2.93 ($9.71) 3.02 ($10.00) $.22 cow/d $.29 cow/d

Lactose (lbs) 4.72 ($2.21) 4.76 ($2.23) 4.81 ($2.25) $.04 cow/d $.02 cow/d

Program Cost $.0130 cow/d $.0325 cow/d $.0295 cow/d $.0165 cow/d -$.003 cow/d

Projected Economics:

Milk pricing based on N.E. Milk Order as of Dec. 31st, 2011

Key Value Points:

• Fully defined crystalline structure

• Increased metal potency

• Maintains essential nutrient potency in the supplement / feed

• Rumen By-Pass– Increased rumen function (DM digestibility)

• Crystalline structure provides for sustained release and improved absorption of the mineral in the intestinal tract

• Increased relative bioavailability (equivalent or better than best OTMs)

– Greater essential mineral absorption drives cow productivity and health

– Ability to reduce total mineral fed, reducing cost & environmental compliance

The only cost effective means to completely replace Trace Mineral sulfate / oxides forms in the diet

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Thank You!