croatia - national report regional workshop 2: improving capacity for diagnosis of fish and mollusc...
TRANSCRIPT
Croatia - National report
Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Mollusc Diseases Banja Luka, Bosnia and Herzegovina, 20-24 October 2013,
Hotel Palas
Production data 2012
Today Croatia reaches production of 16.000 tons acuaqulture products:
4.400 tons carp and trout (šaran i pastrva), 4.000 tons tuna, 4.000 tons sea bass &sea brem (lubina i komarča)3.500 tons shellfish mussels and oysters (dagnji i
kamenica)
Movement/transfers EU – certificate - TRACES Internal market – health certificate
Importation of eggs/fry/live fish After accession, Denmark No imports from BiH!!!
Movement of live animals
Diseases recorded in the country
Viral diseases VHS
Bacterial diseases Aeromonas hydrophila, Vibrio anguillarum, Tenacibaculum
maritimum, Flavobacteruim psychrophila, Yersinia ruckeri,
Parasitic diseases Infection with Marteilia refringens Sea bass/bream – Sea lice
Diagnostic capacity in the country
Aquatic animal diseases laboratory within country
HVI Zagreb, National reference laboratory
Description (diagnostic, research, education) VETERINARY UNIVERSITY ZAGREB INSITUTE RUĐER BOŠKOVIĆ INSTITTUE FOR OCEANOGRPAHY AND FISHERIES
Scope and species interest Fish – freshwater, marine Molluscs Crustacean
Level of diagnostic in Croatia
Level of diagnostic:Level 1: Gross observation, general necropsy
(external, internal)Level II: Bacteriology, Parasitology, Mycology,
HistologyLevel III: Cell lines, Immunological tests,
TEM, Molecular techniques
Analysis of diagnostic capacity
List of diseases and diagnostic methods used
VHS, IHN, IPN,KHV,SVC – clinical observation, detection of gross changes, histological observation, isolation of virus on cell culture, identification by ELISA, IFAT, RT-PCR, qRT-PCR, gene sequencing
Furunculosis, Vibriosis, Yersiniosis, Tenacibaculosis – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification, serological identification, PCR, sequencing.
Mollusc diseases – citology, histology, PCR, sequencing
Surveillance of nationally listed diseases
Surveillance and diagnosis are conducted only for pathogens listed in OIE and EU, on species present in Croatia
Exception ISA on trout OSHV1 – in Ostrea edulis White spot disease in crustaceans
Surveillance and monitoring of other pathogens
Targeted pathogens and aquaculture species
VHS/IHN – salmonids KHV – carp Infection with Bonamia ostreae – Oysters Infection with Marteilia refringens – Mussels,
oysters
Study of abnormal mortality
Mortality description Mortalities reported by fisherman
Mortalities recorded in 2013 – Eustrongyloides
Suspect case of KHV – put and take fisheries – ruled out
Conclusions and perspectives
Inroduction of new diagnostic tehniques – diagnostic of white spot disease, monitoring/antimicrobial resistance, further improvment of control and diagnostic tehniques in marine fish
Education of laboratory staff, proficiency test, education of governemnt officals in aquatic animal health issues