crdf bactericide survey report jan 2019 · pr2016 ba_may2016 adjuvant_ may2016 ba_jun2016....
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CRDF Bactericide Survey Report Jan 2019
CRDF survey of the commercial use of bactericides in Florida citrus. (2016/2017 and 2017/2018).
CRDF staff approached growers in the Central Ridge, Indian River, and Southwest FLA total of 48 cooperator locations with various aged trees, rootstocks and scions.
Staff set up field trials over 2 years Data: Fruit yield (kg/tree), fruit drop (%), PCR cycle threshold count (CT),
and visual disease index score (DI).
1
2
5529292963342776111111101111910113161610101010 26
48 locations Table 1Tree age (years)
Con’t …48 total locations
3
Var / Location Age/ RtSt BA_Mar2016Adjuvant_Mar2016 BA_Apr2016
Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016 BA_Jun2016
BA_Jul2016
BA_Aug2016
Adjuvant_Aug2016
BA_Sep2016
BA_Oct2016
BA_Nov2016
BA_Dec2016
BA_Jan2017
Adjuvant_Jan2017
BA_Feb2017
BA_Mar2017
Adjuvant_Mar2017
BA_Apr2017
Adjuvant_Apr2017
BA_May2017
Adjuvant_May2017
BA_Jun2017
Adjuvant_Jun2017
BA_Jul2017
BA_Aug2017
BA_Sep2017
BA_Oct2017
BA_Nov2017
BA_Dec2017
BA_Jan2018
BA_Feb2018
Adjuvant_Feb2018
BA_Mar2018
Adjuvant_Mar2018
BA_Apr2018
Adjuvant_Apr2018
BA_May2018
Adjuvant_May2018
BA_Jun2018
Adjuvant_Jun2018
Grfrt 1/Riv 5 / SO Mycoshield LI-700 FireLine LI-700 FireLine LI-700 FireWall LI-700Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
Mycoshield
ZnSulphate
BA_Mar2016Adjuvant_Mar2016 BA_Apr2016
Adjuvant_2016 BA_May2016
Adjuvant_May2016
BA_June2016
BA_July2016
BA_Aug2016
Adjuvant_Aug2016
Grfrt 3/Riv 5 / SO Mycoshield LI-700 FireLine LI-700 FireLine LI-700 FireWall LI-700
BA_Apr2016Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016 BA_Jun2016
Adjuvant_Jun2016
BA_Jul2016
Adjuvant_Jul2016
Grfrt 5/Riv 29 / Swg Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700
BA_Apr2016Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016 BA_Jun2016
Adjuvant_Jun2016
BA_Jul2016
Adjuvant_JuL2016
Grfrt 7/Riv 29 / Swg Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700
BA_Apr2016Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016 BA_Jun2016
Adjuvant_Jun2016
BA_Jul2016
Adjuvant_Jul2016
Grfrt 8/Riv 29 / Swg Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700 Mycoshield LI-700
BA_April2016
Adjuvant_May2016 BA_May2016 BA_June20
16 BA_July2016 BA_Aug2016
Adjuvant_Aug2016
Ham 3/SW 6 / Carr FireLine Tactic FireLine Tactic
BA_May2016Adjuvant_May2016 BA_Jun2016
Adjuvant_Jun2016
BA_JuL2016
Adjuvant_JuL2016
BA_Aug2016
Adjuvant_Aug2016
Ham 4/ Riv 33/ SO FirelineKeyplex-445 FireWall Oil-435 FireWall Oil-435 FireLine Oil-435
BA_Apr2016Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016
BA_June2016
BA_July2016
Adjuvant_July2016
BA_Aug2016
Adjuvant_Apr2016
BA_Sep2016
BA_Oct2016
BA_Nov2016
Adjuvant_Nov2016
Ham 5/SW 4 / Swg Mycoshield LI_700 Mycoshield LI_700Mycoshield LI_700
Mycoshield LI_700
Mycoshield LI_700
BA_Apr2016Adjuvant_Apr2016 BA_May2016
Adjuvant_May2016 BA_Jun2016
BA_Jul2016
BA_Aug2016
BA_Sep2016
BA_Oct2016
Adjuvant_Oct2016
Ham 8/ SW 6 / Swg FireLine Tactic FireWall Tactic FireLine N_Sure
BA_Mar2016Adjuvant_Mar2016 BA_Apr2016
Adjuvant_Apr2016 BA_May2016
BA_Jun2016 BA_Jul2016
Adjuvant_Jul2016
BA_Aug2016
BA_Sep2016
Adjuvant_Sep2016
BA_Oct2016
Adjuvant_Oct2016
BA_Nov2016
BA_Dec2016
BA_Jan2017
BA_Feb2017
Adjuvant_Feb2017
BA_Mar2017
Adjuvant_Mar2017
BA_Apr2017
Adjuvant_Apr2017
Ham 12/Rid 11/Cleo Mycoshield Pas-800 FireLine Pas-800 Mycoshield Pas-800Mycoshield LI-700 FireWall LI-700
Mycoshield Pas-800
Mycoshield Pas-800
Mycoshield Pas-800
BA_Mar2016Adjuvant_Mar2016 BA_Apr2016
Adjuvant_Apr2016 BA_May2016
BA_June2016
Adjuvant_June2016
BA_July2016
Adjuvant_July2016
BA_Aug2016
BA_Sep2016
Adjuvant_Sep2016
BA_Oct2016
Adjuvant_Oct2016
BA_Nov2016
BA_Dec2016
BA_Jan2017
BA_Feb2017
Adjuvant_Feb2017
Ham 16/ SW 11 / Kuh FireLine Sil-MES FireLine Sil-MES FireLine Sil-MESMycoshield LI-700
Mycoshield LI-700 FireWall LI-700
Mycoshield LI-700
BA_Apr2016 Adjuvant_Apr2016 BA_May2016 BA_Jun201
6 BA_Jul2016 BA_Aug2016
Adjuvant_Aug2016
MidSwt 2/SW 9 / Carr FireLine Tactic FireLine Tactic
BA_Apr2016Adjuvant_A
pr2016 BA_May2016Adjuvant_May2016 BA_Jun2016
Adjuvant_Jun2016
BA_Jul2016
BA_Aug2016
Adjuvant_Aug2016
BA_Sep2016
BA_Oct2016
Adjuvant_Oct2016
BA_Nov2016
Adjuvant_Nov2016
BA_Dec2016
BA_Jan2017
BA_Feb2017
Adjuvant_Feb2017
BA_Mar2017
BA_Apr2017
BA_May2017
BA_Jun2017
Adjuvant_Jun2017
BA_Jul2017
BA_Aug2017
Adjuvant_Aug2017
BA_Sep2017
BA_Oct2017
Adjuvant_Oct2017
BA_Nov2017
BA_Dec2017
BA_Jan2018
BA_Feb2018
BA_Mar2018
BA_Apr2018
Adjuvant_Apr2018
BA_May2018
Adjuvant_May2018
Val 4/ SW 16 / Kuh FireLine Tactic FireWall Mycoshield 435_Oil FireLine 435_Oil Mycoshield LI-700 FireWall Tactic FireLine Exit FireWall 435_Oil FireWall 435_Oil FireLine Exit FireWall Response FireLine Response
BA_May2016Adjuvant_May2016 BA_Jun2016
BA_Jul2016
Adjuvant_Jul2016
BA_Aug2016
BA_Sep2016
Adjuvant_Sep2016
BA_Oct2016
BA_Nov2016
Adjuvant_Nov2016
BA_Dec2016
BA_Jan2017
Adjuvant_Jan2017
BA_Feb2017
BA_Mar2017
BA_Apr2017
BA_May2017
BA_Jun2017
Adjuvant_Jun2017
BA_Jul2017
BA_Aug2017
Adjuvant_Aug2017
BA_Sep2017
BA_Oct2017
BA_Nov2017
BA_Dec2017
BA_Jan2018
Adjuvant_Jan2018
Val 12/ SW 26 /Carr FireLine KeyPlex445 FireWallKeyPlex44
5Mycoshiel
d LI-700 FireWallPT_Surf_90_10
Mycoshield LI-700 FireWall LI-700 FireLine LI-700 FireWall LI-700
BA_Mar2016Adjuvant_Mar
2016 BA_Apr2016Adjuvant_A
pr2016 BA_May2016Adjuvant_May2016 BA_Jun2016
BA_Jul2016
BA_Aug2016
Adjuvant_Aug2016
BA_Sep2016
BA_Oct2016
BA_Nov2016
BA_Dec2016
BA_Jan2017
Adjuvant_Jan2017
BA_Feb2017
BA_Mar2017
BA_Apr2017
Adjuvant_Apr2017
BA_May2017
BA_Jun2017
Adjuvant_Jun2017
BA_Jul2017
BA_Aug2017
BA_Sep2017
BA_Oct2017
Adjuvant_Oct2017
BA_Nov2017
BA_Dec2017
BA_Jan2018
BA_Feb2018
Adjuvant_Feb2018
BA_Mar2018
BA_Apr2018
Adjuvant_Apr2018
Val 17/ Rid 21 / Swg Mycoshield LI-700 Mycoshield Orocit FireLineMycoshiel
dMycoshiel
d OrocitMycoshiel
d OrocitMycoshiel
d EnhanceMycoshiel
dDW_Surfactant
Mycoshield
DW_Surfactant
Mycoshield
BA_Mar2016Adjuvant_Mar2016 BA_Apr2016
BA_May2016
Adjuvant_May2016
BA_June2016 BA_July2016
Adjuvant_July2016
BA_Aug2016
BA_Sep2016
Adjuvant_Sep2016
BA_Oct2016
Adjuvant_Oct2016
BA_Nov2016
BA_Dec2016
BA_Jan2017
BA_Feb2017
BA_Mar2017
Adjuvant_Mar2017
BA_Apr2017
Adjuvant_Apr2017
BA_May2017
Adjuvant_May2017
BA_June2017
Adjuvant_June2017
BA_July2017
BA_Aug2017
BA_Sep2017
Adjuvant_Sep2017
BA_Oct2017
Adjuvant_Oct2017
BA_Nov2017
BA_Dec2017
BA_Jan2018
BA_Feb2018
BA_Mar2018
Adjuvant_Mar2018
BA_Apr2018
Adjuvant_Apr2018
Val 38/ Rid 20+/ Kuh Mycoshield Pas-800 FireLine Pas-800 Mycoshield Pas-800Mycoshiel
d LI-700 FireWall LI-700Mycoshiel
d Pas-800Mycoshiel
d Pas-800Mycoshield Pas-800
Mycoshield Pas-800
Mycoshield Pas-800
Mycoshield LI-700
Mycoshield LI-700
Mycoshield LI-700
Bactericide treatment = Region, tree age, rootstocks, scions,
bactericide products, timing, dosage, number of applications, and tank mixincluding choice of adjuvants
Data Year 1 + Year 2
4
Single block design
4 x 5 sentinel trees
5
2 Block Design
2.6 2.3
6
Fruit Yield (40 kg = 90 lb box) 16 locations w/ 2 years:
45
293233
3.6 > 32.6 > 2.3
2.4 2.8
.75 1.1
Tree age
Inc. yieldNot yr 2 w low yield
dec. yieldboth yrs
ns.
Treatment
<
<
Bactericide
Inc yield yr 2
box
7
(Table 2 con’t) Fruit Yield (40 kg = 1 box) in both years
4.6 > 3.6 20+ yrs dec. yieldns in yr 2 lower yd
Treatment
ns.
Overall, of 16 two year data locations, Bact. Trt inc. yield in 2 sites, 3 sites dec. yield Control averaged 1.6 boxes vs. Bactericide Trts 1.5 boxes (ns)
dec.yield
>
>
Fruit Yield (40 kg = 1 box) in Year 1 (2016-17); 15 locations, Bact. Trt. reduced yield
<9
29
26
dec.
Inc.
3.4 2 boxes
2.7 3.4
1.5 0.9
Treatment
ns
ns
Age
Overall 15 sites Year 1: Control averaged 2 boxes vs 1.8 boxes* TreatedYear 2 only 12 locations: 1.5 Control vs. 1.52 boxes NS
9
% Fruit Drop from 2 years data; 11 locations, treatment dec. drop at 2 sites, inc at 1Overall, avg 33 % : No impact of bactericide
>
><
dec. dropinc.
dec drop, Yd, T2)
Treatment
454
Age
ns
%
Year 1
10
% Fruit Drop in Year 1 only; 15 sites: avg 16 % drop Cont vs 18% Treated *
< Trt inc drop
Trt inc drop
ns
5
10
26
Treatmentns
<
< Trt inc drop*
%
11
% Fruit Frop in Year 2 only; of 17 locations, 2 inc. drop, 1 dec; 53% No Trt impact.
dec. dropInc. drop
Inc. dropns
><
<
Treatmentns
12
Visual Disease Index (DI) significant (P< 0.10) impacts only
><<<<<<<<><>><><<<><
Improved ( Y,Dr)Worse
appearance
worseImproved
ImprovedImproved
Improved
ImprovedWorse
Treatment
Of 20 locations impacted, 14 lnc. DI6 dec. DI
All locations (48), 2 yrs = 96 DIs: Overall, DI = 22 control vs. 22.2 *for treated trees.
76 sites had no treatment effects
13
PCR CT values significant impacts only (P < 0.01)39 sites had 2 years, CT avg 29 (NS) Year 1 only: 5 dec. titer and 6 inc. titer In Year 2; no dec titer and 2 inc. titer
>>><>><<><>><
Treatment
Inc. titer> Inc.> Inc.
Dec. titer> Inc.> Inc.
Dec. titerDec. titer
Inc. titer
Dec. titerInc. titer
> Inc. Dec. titer
No 40s
14
Effects of Bactericide applications on:Yield from 16 locations with 2 years of data (Table 2):
Bactericide Trts increased yield at 2 sites, decreased at 3 sites; the rest NSOverall: Control averaged 1.6 boxes vs. 1.5 boxes Treated (ns) Yield Yr 1 only: 15 sites: yield increased at 1 site, Yld dec. at 2 sites (Table 4)Control Avg: 2 boxes vs 1.8 boxes*Treated; Year 2 (1.5 vs. 1.52 boxes NS
Fruit Drop: 2 years, 11 sites, 2 dec, 1 inc. drop. (Table 5) Avg 33% No Trt effectYear 1 only, 15 sites, 3 inc. drop; Avg: 16 % drop Control vs. 18% Treated *Year 2 only; 17 sites, 2 inc. drop, 1 dec. Average 53 %, no Trt effect (T 6).
DI : Overall 48 sites, 2 yrs = 96 DIs = 22 control vs. 22.2 * for treated trees. 76 sites had no bactericide trt effects on DI Of 20 sites impacted 14 looked worse, 6 appeared better (Table 7).
PCR CT : 39 locations had CTs for both years, Avg CT = 29 (ns); Of 13 locations impacted, 8 higher titer, 5 had decreased titer (Table 8)
.