Craigiebuckler, Aberdeen, AB15 8QH, UK

RECIPE Final Aberdeen Progress meeting

29-31/05/2006WP 03: Structure and diversity of the

microbial loop

UK progressRebekka ArtzEileen Reid

Stephen Chapman

D10 Genetic diversity of fungal communities

• Completed for WP1 – biogeographical effects shown, but within each country fungal communities are more similar to advanced/intact stages with each regeneration stage

• 1st paper submitted to Environmental Microbiology on the fungal diversity of Scottish bogs (as introduction paper – this includes sequencing data)

• 2nd paper on structure of WP1 data in prep. Still needs some stats (Mantel tests for vegetation/location data exploration)

• WP2 samples very problematic, still working on these….

CV1 (53.2 %)

-4 -3 -2 -1 0 1 2 3 4

CV2

(25.

3 %

)

-3

-2

-1

0

1

2

3

4ch 1 vs ch2 fb 1 vs fb2 fi 1 vs fi2 fr 1 vs fr 2 sc 1 vs sc 2

CV 1(65%)

-6 -4 -2 0 2 4

CV

2 (2

6 %

)

-3

-2

-1

0

1

2

3

PATCHYEARLYADVANCEDINTACT

CV 1(52.0 %)

-4 -3 -2 -1 0 1 2 3

CV

2 (3

4 %

)

-3

-2

-1

0

1

2

3

4

EV.WEV.DCRSPBARE

CV 1 (53 %)

-4 -3 -2 -1 0 1 2 3 4

CV

2 (3

4 %

)

-4

-3

-2

-1

0

1

2

3

4

BAREEARLYADVANCEDINTACT

CV 1(60 %)

-4 -3 -2 -1 0 1 2 3 4

CV

2 (3

2%)

-4

-3

-2

-1

0

1

2

3

BARESPEA/SPADVANCED

CH FI

FR SC

?

III. Fungal community structure analysis

0%

20%

40%

60%

80%

100%

A B C DSite

Rel

ativ

e ab

unda

nce

of fu

ngal

phy

la NFUFCZBA

Changes in the overall composition with regeneration stage

Table 4: Distribution of strictly assigned OTU types occurring more than once, by peatland regeneration stage and horizon. Bold OTU are clones related to Sarcoleotia turficola (see text). OTU # 1 5 11 15 19 22 25 29 67 70 Site Horizon 2

3 2 4 3 2 6 1

A

8 1 1 3 4 1 1 4 6 1 1

B

8 1 3 3 1 4 1 2 2 6 2 5

C

8 1 2 3 1 4 6 3 1 1

D

8 1 1 2

Sarcoleotia turficola: a rare fungus growing in association with Sphagnum spp.

Potential indicators of regeneration?• 68% of all clones were not identifiable to Genus or Order (!) level

Phialocephala spp: fungi growing as root endophytes/saprophytes in cool wetlands