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  1. 1. CONSIDERSAFETY: PreparingSamplesforCryopreservation
  2. 2. LOWTEMPERATUREPROTECTION Whenhandlingcryopreservedsamplesandcold temperatures,stashouldwear: Paddedgauntletsforhand/armprotection Long-sleevedlabcoats Closed-toefootwear Afull-facevisor
  3. 3. SAMPLEPREPARATION Additivesareaddedtocellswhenpreparingthemfor cryopreservationtoprotectthecellsfromdamage: dimethylsulfoxide(DMSO) polyethyleneglycol glycerol DMSOiseasilyabsorbedthroughskin.Long-sleevedlabcoats, faceprotectionandglovesshouldbewornduringpreparation.faceprotectionandglovesshouldbewornduringpreparation.
  4. 4. BIOHAZARDSANDCRYOPRESERVEDSAMPLES Whendealingwithbiosamples,stashouldtakethefollowing precautions: Beawareofthebiosafetylevels Properlylabelsamples Practicetheappropriatesafetyproceduresforthegiven samples
  5. 5.
  6. 6. CONTAMINATION Pathogenscansurvivethelowtemperatures.Itisimportant towipetheoutsideofvialswithgauzesoakedinalcohol beforeopening. Wherecontaminationissuspected,stashouldfollow appropriatedecontaminationproceduresforcleaning liquidnitrogenstoragetanks.
  7. 7. SOURCES ConsiderSafety:PreparingSamplesforCryopreservationBlog Shutterstock

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Role and Objectives of Cryopreservation Programscgn.websites.wur.nl/seminars/Workshop20100601/Role... · numbers of samples needed (e.g. Conservation planner) will be discussed elsewhere

Preparing 2–5kb Samples for Mate Pair Library Sequencingzazil.ibt.unam.mx/usmb/wp-content/uploads/2016/05/... · Preparing 2–5kb Samples for Mate Pair Library Sequencing Recovered

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Advances in sperm cryopreservation of samples collected by ...digital.csic.es/bitstream/10261/147204/1/electroejacublancelti.pdf · Advances in sperm cryopreservation of samples collected

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