confoscan images to follow
DESCRIPTION
Confoscan Images to Follow. MICROSCOPIC EXAMINATION. - PowerPoint PPT PresentationTRANSCRIPT
Confoscan Images to Follow
MICROSCOPIC EXAMINATION
• Two H & E, two PAS, one B & B, one GMS, one Alcian blue, one Masson Trichrome and one Congo red stained slides are examined. Sections through the specimen show a piece of cornea with a variably thickened epithelium. There is subepithelial fibrosis with Bowman’s layer appearing disrupted; fragments of which are seen in the stroma. The stroma also shows red-staining material on Masson’s Trichrome. A few lymphocytes and plasma cells are also noted. Congo red and Alcian blue stains are negative. B & B and GMS stains are negative for any organisms associated with infectious crystalline keratopathy. PAS stain confirms the above findings.
Thickened epithelium
Subepithelial fibrosis
Fragments of Bowman’slayer
Red-staining material on Masson’s Trichrome
PAS
Congo red and Alcian blue stains are negative.
B & B and GMS stains are negative for any organisms.
Congo red
GMS
Alcian blue
B & B
Diagnosis
1) Subepithelial fibrosis.
2) Red-staining stromal deposits on Masson’s Trichrome.
Corneal epithelium, keratocytes, and endothelium should stain red; however, the most common stromal material which stains this way is hyaline. If a different material is suspected one could consider further studies.