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Concepts of Neuronal Network Function December 2, 2011
Lawrence N Eisenman MD PhD
Adult Epilepsy Center
Washington University in St. Louis
American Epilepsy Society | Annual Meeting
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Disclosure
Name of Commercial
Interest
Johnson & Johnson, Medtronic, Merck, Pfizer
Type of Financial Relationship
Stock Ownership
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Symposium Learning Objectives
• Apply knowledge of motor-based signs and symptoms in
epilepsy with localization of partial seizures.
• Correlate common ictal semiology findings with
pathways of seizure propagation.
• Understand and, as appropriate, apply MRI-based
functional connectivity techniques in the diagnosis of
epileptic seizures.
• Utilize concepts of neuronal network function and
associated applications in MRI-based functional
connectivity techniques in evaluating patients with
epilepsy
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Neuronal network activity is
determined by:
• Intrinsic neuronal properties
– Neuronal morphology/passive properties
– Types and distributions of ion channels
• Synaptic properties
– Types and distributions of synaptic channels
• Pattern of network connectivity
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Network properties and activity
Netoff et al, J Neurosci 24:8075 2004
“Normal” “Pathologic”
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How do we think about
connectivity data?
Is there a way categorize and/or
quantify connectivity data?
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Learning Objectives
• Understand the basic concepts of graph theory.
• Recognize the potential utility of graph theory for advancing our understanding of the functioning of neuronal networks in epilepsy and other neuropsychiatric diseases.
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Graph Theory
• Graph theory is a branch of mathematics
devoted to the study of graphs.
• A graph is defined as a collection of nodes
and a collection of the edges
(connections) between nodes.
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Graph theory is used in a wide
variety of disciplines
• Social networks
• Computer networks
• Gene networks
• Neuroscience
Figure courtesy of Luigi Maccotta MD PhD
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Basic Graph Metrics • Degree = number of edges connected to a particular
node
– Hub = node with high degree
• Degree distribution = probability of a node having a
particular degree (often shown as a histogram)
• Average shortest path length = number of edges in the
shortest past between two nodes averaged over all pairs
of nodes
– Betweenness centrality = The ratio of number of shortest paths
between nodes that include the node to the total number of
shortest paths.
• Clustering coefficient = fraction of neighbors of a node
that are connected to each other Bullmore and Sporns, Nat Rev Neurosci 10:186 2009
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Small World Graph
• High average
clustering coefficient
• Low average path
length
• Good compromise
between computational
efficiency and wiring
economy
Watts and Strogatz, Nature 393:440 1998
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0.1
1
pro
ba
bil
ity
12 3 4 5 6 7 8 9
10
number of edges
Scale Free Graph
• Degree distribution
follows a power law
– P(k) ~ k-
• Small number of
highly connected
nodes called hubs
• Especially resistant to
random loss of
individual nodes Barabasi and Albert, Science 286:509 1999
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Neuronal networks in the brain
are organized as:
• Small world networks?
– Functional neuroimaging suggests
connectivity between brain regions is
consistent with a small world network.
– There are also highly connected hub regions.
Bullmore and Sporns, Nat Rev Neurosci 10:186 2009
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Neuronal networks in the brain
are organized as:
• Scale free networks?
– Optical imaging in hippocampal slices
suggests neuronal networks in CA3 are
consistent with scale free networks (Bonifazi et al,
Science 326:1419 2009).
– Multi-electrode array recording in cultured
neurons suggests that neurons spontaneously
form scale free networks (Eisenman et al, in preparation).
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Functional connectivity in temporal
lobe epilepsy
• 7 of 8 L mTLE patients showed decreased
connectivity in the left temporal lobe (Bettus et
al, Hum Brain Mapp 30:1580 2009)
• R mTLE patients showed decreased
connectivity in both mesial temporal lobes (Zhang et al, Brain Res 1323:152 2010)
• mTLE patients showed increased
connectivity within the mesial temporal
lobes (Liao et al, PLoS One 5:e8525 2010)
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Graph theory may be useful for
identifying MTLE patients • Functional connectivity measured using
resting state fMRI in 52 controls and 16
MTLE patients.
• Graph theory measures calculated for each
subject.
• Data was used to construct a classifier with
77% sensitivity and 84% specificity calculated
with „leave one out‟ cross validation.
Zhang et al, J Neurosci Methods 199:129 2011
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Graph theory may be useful for
identifying the ictal onset zone
• 25 patients underwent intracranial
electrocorticography for refractory
neocortical onset epilepsy. 15 underwent
resection and 5 became seizure free.
• Graphs were constructed by considering
recording contacts as nodes and using the
directed transfer function calculated during
seizures to identify edges. Wilke et al, Epilepsia 52:1528 2011
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Graph theory may be useful for
identifying the ictal onset zone
• Betweenness centrality was calculated for
each node in the network and regions with
high betweenness centrality during
seizures were identified.
• Seizure free patients had more overlap
between regions of high betweenness
centrality and clinically identified ictal
onset zones. Wilke et al, Epilepsia 52:1528 2011
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Functional connectivity in other
neuropsychiatric diseases
• Alzheimer‟s disease is associated with
disruption of hubs which are highly
correlated with areas of high amyloid
deposition.
• Schizophrenia is associated with an
overall decrease in connectivity with less
highly connected hubs.
Sporns, Front Comput Neurosci 5:5 2011
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Hypothesis:
Some neuropsychiatric diseases
are associated with an increased
“randomization” of brain networks
Sporns, Front Comput Neurosci 5:5 2011
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Summary and impact on clinical
care and practice
• Neuronal network activity is dependent on
neuronal properties, synaptic properties and the
pattern of network connectivity.
• Graph theory can be used to classify and
quantify connectivity data to advance our
understanding of epilepsy and other
neuropsychiatric diseases.
• Graph theory may eventually be useful for the
diagnosis and treatment of epilepsy.