computational analysis of metabolic networkscomputational analysis of metabolic networks ralf steuer...
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Computational Analysis of Metabolic Networks
Ralf SteuerHumboldt-University Berlin, GermanyInstitute of Theoretical Biology (ITB)
CzechGlobeGlobal Change Research Centre, Brno, CZ
Fakulta informatiky MUpondělí 14. 5. 2012, 14:00
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A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
outline
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A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
(1) Transcriptional regulation: from DNA to protein
DNA
protein
transcription/translation
outline
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A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
(2) Posttranscriptional regulation: (de)activation of proteins
DNA
protein
protein activity
signaling networks
transcription/translation
outline
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A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
(3) Cellular metabolism: energy and growth
DNA
protein
protein activity
cell metabolism
signaling networks
building “infrastructure”
transcription/translation
outline
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A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
(4) Cellular physiology: division, motility, etc ...
DNA
protein
protein activity
cell metabolism
physiology andbehavior
signaling networks
building “infrastructure”
transcription/translation
outline
![Page 7: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/7.jpg)
A living cell is a complex dynamic systeminvolving many hierarchies of regulation:
(4) Cellular physiology: division, motility, etc ...
DNA
protein
protein activity
cell metabolism
physiology andbehavior
signaling networks
building “infrastructure”
transcription/translation
outline
![Page 8: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/8.jpg)
A living cell is a complex dynamic systeminvolving many hierarchies of regulation:Aim: Computational Modeling of Cellular Processes
DNA
protein
protein activity
cell metabolism
physiology andbehavior
signaling networks
building “infrastructure”
transcription/translation
outline
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Computational modeling as a tool to understandthe functioning of cellular interactions?
Three main issues:
(1) Why should one care about modeling?(2) How should a (good) model be constructed?(3) And to what end?
the rationale of mathematical modeling
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The rationale of mathematical modelling
Mathematical Models: A method for representation1.1.
Mathematical Models: A method for deduction2.2.
the rationale of mathematical modeling
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Mathematical Models: A method of representationTranscription of properties into a formal representation.Modeling provides a language for representation.1.1.
An example: The basic MichaelisMenten Scheme
The rationale of mathematical modelling
the rationale of mathematical modeling
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A slightly more complicated example:
MFPMFPMNFP2
MNFP1XYZ1
XYZ2
XYZ3
ABC1ABC1 ABCa2ABCa2?
R2D2 inhibition Note that models do notneed tobe 'true'.
The rationale of mathematical modelling
Mathematical Models: A method of representationTranscription of properties into a formal representation.Modeling provides a language for representation.1.1.
the rationale of mathematical modeling
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Mathematical Models: A method for deductionTranslation into emergent properties at the systems level.Mathematical models organize parts into a coherent whole.
2.2.
Facts often only mean little in isolation, but need to be interpretedin terms of a theory or model.
Science is built with facts, as a house is with stones. But a collection of facts is no more a science than a heap of stones is a house. — Henri Poincare
The rationale of mathematical modelling
the rationale of mathematical modeling
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Mathematical Models: A method for deductionTranslation into emergent properties at the systems level.Mathematical models organize parts into a coherent whole.
2.2.
An example: Can this mechanism oscillate?
Translation intoemergent properties
MFPMFPMNFP2
MNFP1XYZ1
XYZ2
XYZ3
ABC1ABC1 ABCa2ABCa2?
R2D2 inhibition
The rationale of mathematical modelling
the rationale of mathematical modeling
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The modeling relation according to Casti:
The rationale of mathematical modelling
the rationale of mathematical modeling
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“There is a zoo of mathematical models in the literature. Many of these appear to have little purpose other than calculating numbers which conform reasonably to experimental data. This is, in itself, not a distinguished endeavor; it is not particularly difficult, and it teaches little. ... Modeling is relatively meaningless without explicit definition, at the outset, of its purpose”
J. E. Bailey (19442001) in Biotechnol. Prog. 14:820, 1998
The rationale of mathematical modelling
the rationale of mathematical modeling
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Computational models of metabolic networks
models of metabolic networks
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Computational models of metabolic networks
“food”
proteincarbohydrates
fat
“useful”energy
building blocks
models of metabolic networks
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Computational models of metabolic networks
“food”
proteincarbohydrates
fat
“useful”energy
building blocks
Cellularconstituents
maintenance
models of metabolic networks
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Computational models of metabolic networks
Copyright 2004 by Alberts, Bray, Johnson, Lewis, Raff, Roberts, Walter.Garland Publishing: Taylor Francis Group.
models of metabolic networks
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The “bowtie” structure of metabolism:
A bow-tie
models of metabolic networks
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The “bowtie” structure of metabolism:
A Complex Network of Interactions:
• Biochemical reactions
• Metabolic compounds
• Regulatory interactions
Applications of Relevance:
Biotechnology
Diseases and medical applicationsA link from genotype to phenotype
models of metabolic networks
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The network of enzymecatalyzed biochemical reactions:
Enzymes are biological molecules that catalyze (i.e., increase the rates of) chemical reactions. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Since enzymes are selective for their substrates and speed up only a few reactions from among many possibilities, the set of enzymes made in a cell determines which metabolic pathways occur in that cell.
Like all catalysts, enzymes work (only) by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction.
from: WIKIPEDIA
models of metabolic networks
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Enzymecatalyzed reactions:
Reaction: A B (exergonic ∆G<0)
models of metabolic networks
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Enzymecatalyzed reactions:
Reaction: A B (exergonic ∆G<0)
Reaction: C D (endergonic ∆G>0)
models of metabolic networks
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Enzymecatalyzed reactions:
Reaction: A B (exergonic ∆G<0)
Reaction: C D (endergonic ∆G>0)
Enzymes can couple processes
Reaction: C D (∆G>0)
Reaktion:ATP ADP + “energy” (∆G<0)
C + ATP ADP + D (∆G<0)
models of metabolic networks
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Enzyme mechanisms:
models of metabolic networks
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Enzyme mechanisms and rate equations:
Translation into mathematical model: rate equations
models of metabolic networks
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MichaelisMenten Kinetics:
Translation into mathematical model: rate equations
models of metabolic networks
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MichaelisMenten Kinetics:
Translation into mathematical model: rate equations
Maximal velocity
models of metabolic networks
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Translation into mathematical model: rate equations
MichaelisMenten constant
Maximal velocity
MichaelisMenten Kinetics:
models of metabolic networks
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MichaelisMenten Kinetics:
MichaelisMenten constant
Maximal velocity
models of metabolic networks
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More complicated enzymekinetic mechanisms ...
models of metabolic networks
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More complicated enzymekinetic mechanisms ...
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites(2) Assign rate equations to each reaction
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites(2) Assign rate equations to each reaction(3) Assign values to all parameters
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites(2) Assign rate equations to each reaction(3) Assign values to all parameters(4) Solve the system numerically
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites(2) Assign rate equations to each reaction(3) Assign values to all parameters(4) Solve the system numerically(5) DONE!
models of metabolic networks
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Building a model of cellular metabolism ...
(1) Assemble the building blocks: reactions and metabolites(2) Assign rate equations to each reaction(3) Assign values to all parameters(4) Solve the system numerically
Unfortunately not so easy ...(1) The 'Building blocks' are often unknown.(2) Parameters are difficult to estimate(3) Numerically complex
models of metabolic networks
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A few words on nomenclature:
Reaction:
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A few words on nomenclature:
Reaction:
Stoichiometry:
Rate equation:
models of metabolic networks
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A few words on nomenclature:
Organize the network into a set of differential equations:
models of metabolic networks
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A few words on nomenclature:
Organize the network into a set of differential equations:
Stoichiometric coefficients
models of metabolic networks
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A few words on nomenclature:
Organize the network into a set of differential equations:
Rate equations
models of metabolic networks
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A few words on nomenclature:
Organize the network into a set of differential equations:
In matrix notation:
models of metabolic networks
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Organize the network in a set of differential equations:
models of metabolic networks
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Organize the network in a set of differential equations:
models of metabolic networks
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Organize the network in a set of differential equations:
models of metabolic networks
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The stoichiometry is increasingly known:
models of metabolic networks
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The stoichiometry is increasingly known:
models of metabolic networks
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The stoichiometry is increasingly known:
models of metabolic networks
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Other resources:
models of metabolic networks
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Modelling metabolic networks
models of metabolic networks
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Modelling metabolic networks ... has many facettes:
models of metabolic networks
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Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis● Static description● No kinetic parameters● Topological properties
Quantita tive M
o dels
models of metabolic networks
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Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis● Static description● No kinetic parameters● Topological properties
Quantita tive M
o dels
the substrate graph
models of metabolic networks
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Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
An example: The S. cerevisiae metabolic network
M = 810 vertices (metabolites)R = 843 reactionsE = 3419 edges
models of metabolic networks
![Page 59: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/59.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
Typical network properties:Pathlength and clusteringDegree distributionHierarchies and modularityRobustness and error tolerance
Advantages:Applicable to largescale systemsRequires only topological information
models of metabolic networks
![Page 60: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/60.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
the degree distribution
Typical network properties:Pathlength and clusteringDegree distributionHierarchies and modularityRobustness and error tolerance
models of metabolic networks
![Page 61: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/61.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
the degree distribution
highly connected metabolites:
glycolysis, TCA cycle (pyruvate, etc ...) cofactors (ATP, NAD, etc ...)
Typical network properties:Pathlength and clusteringDegree distributionHierarchies and modularityRobustness and error tolerance
models of metabolic networks
![Page 62: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/62.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
Typical network properties:Pathlength and clusteringDegree distributionHierarchies and modularityRobustness and error tolerance
highly connected metabolites:
models of metabolic networks
![Page 63: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/63.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Topological Analysis
Quantita tive M
o dels
Typical network properties:Pathlength and clusteringDegree distributionHierarchies and modularityRobustness and error tolerance
Limitations:Only qualitative descriptionNo dynamic propertiesNo specific features of metabolic networks
models of metabolic networks
![Page 64: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/64.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Explicit Kinetic Models● Dynamic description● Kinetic parameters● Differential equations
Quantita tive M
o dels
models of metabolic networks
![Page 65: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/65.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Explicit Kinetic Models● Dynamic description● Kinetic parameters● Differential equations
Quantita tive M
o dels
(1) Assemble the “parts list”: reactions and metabolites(2) Assign rate equations to each reaction(3) Assign values to all parameters(4) Solve the system numerically(5) DONE!
models of metabolic networks
![Page 66: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/66.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Explicit Kinetic Models● Dynamic description● Kinetic parameters● Differential equations
Quantita tive M
o dels
A simple Example:
models of metabolic networks
![Page 67: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/67.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Explicit Kinetic Models● Dynamic description● Kinetic parameters● Differential equations
Quantita tive M
o dels
A typical rate equation:
Example from Chassagnole (2002)
A simple Example:
models of metabolic networks
![Page 68: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/68.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Explicit Kinetic Models● Dynamic description● Kinetic parameters● Differential equations
Quantita tive M
o dels
Then solve system computationally:
models of metabolic networks
![Page 69: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/69.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
A more complex example: Identify best (putative) drugtargets in a model of human metabolism.
By E. Murabito (Manchester)
models of metabolic networks
![Page 70: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/70.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
A more complex example: Identify best (putative) drugtargets in a model of human metabolism.
By E. Murabito (Manchester)
models of metabolic networks
![Page 71: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/71.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
A more complex example: Identify best (putative) drugtargets in a model of human metabolism.
By E. Murabito (Manchester)
Matrix of control coefficients
models of metabolic networks
![Page 72: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/72.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
A more complex example: Identify best (putative) drugtargets in a model of human metabolism.
By E. Murabito (Manchester)
AdvantagesA large number od potential targets can be tested quicklyNo ethical problemsFast resultsCheap
models of metabolic networks
![Page 73: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/73.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
A more complex example: Identify best (putative) drugtargets in a model of human metabolism.
AdvantagesA large number od potential targets can be tested quicklyNo ethical problemsFast resultsCheap
But: Current models not predictive yetToo many unknown parametersToo many unknown interactions
models of metabolic networks
![Page 74: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/74.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
See repositories for explicit kinetic models:
models of metabolic networks
![Page 75: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/75.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
See repositories for explicit kinetic models:
models of metabolic networks
![Page 76: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/76.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
See repositories for explicit kinetic models:
models of metabolic networks
![Page 77: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/77.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
See repositories for explicit kinetic models:
AdvantagesA large number od potential targets can be tested quicklyNo ethical problemsFast resultsCheap
But: Current models not predictive yetToo many unknown parametersToo many unknown interactions
models of metabolic networks
![Page 78: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/78.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
Most research now on the intermediate level!
models of metabolic networks
![Page 79: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/79.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Stoichiometric Analysis (Flux Balance Analysis)● Static description● No kinetic parameters● Quantitative predictions
Exploits constraints in flux distribution
models of metabolic networks
![Page 80: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/80.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Stoichiometric Analysis (FBA)● Static description● No kinetic parameters● Quantitative predictions
Exploits constraints in flux distribution
A simple Example:
models of metabolic networks
![Page 81: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/81.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Stoichiometric Analysis (FBA)● Static description● No kinetic parameters● Quantitative predictions
Exploits constraints in flux distribution
In general only a small fraction of fluxes need to be known:independent = (number fluxes) – (number metabolites)
A simple Example:
models of metabolic networks
![Page 82: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/82.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Stoichiometric Analysis (FBA)● Static description● No kinetic parameters● Quantitative predictions
Exploits constraints in flux distribution
The S. cerevisiae metabolic network
M = 810 vertices (metabolites)R = 843 reaction rates
Optimize according toan objective function
Probably the best approach to date ...
models of metabolic networks
![Page 83: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/83.jpg)
Qualitat ive M
od els
Size of System
Level of Detail
Quantita tive M
o dels
Most research now on the intermediate level!
Extend with features of explicit kinetic modelsInclude quantitative description of dynamicsInclude feedback regulation into the description
Keep the advantages of topological analysis
No knowledge of kinetic parametersComputationally feasible for large networks
No explicit enzymekinetic rate equations
models of metabolic networks
![Page 84: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/84.jpg)
Research at HUBerlin/ITB: Systems Biologyof Cyanobacterial Biofuel Production
Cyanobacterial Biofuel Production
![Page 85: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/85.jpg)
Research at HUBerlin/ITB: Systems Biologyof Cyanobacterial Biofuel ProductionFossil fuels (mainly petroleum, coal, natural gas) account for 80%-90% of the current world energy demand.
Fossil fuels account for 95% of transportation fuels.
Cyanobacterial Biofuel Production
![Page 86: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/86.jpg)
Fossil fuels (mainly petroleum, coal, natural gas) account for 80%-90% of the current world energy demand.
Fossil fuels account for 95% of transportation fuels.
Alternatives are urgently needed!Wishlist: Renewable, cheap, abundant, biodegradable, etc ...
Cyanobacterial Biofuel Production
![Page 87: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/87.jpg)
Biofuel production from biomassa) vegetable oil/biodiesel (soybean, palm, rapeseed)b) bioalcohol from sugar cane and starch (corn, maize)
+ some others (charcoal, biogas, etc ...)
maize (wiki)palm plantation (wiki)
Cyanobacterial Biofuel Production
![Page 88: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/88.jpg)
Biofuel production from biomassa) vegetable oil/biodiesel (soybean, palm, rapeseed)b) bioalcohol from sugar cane or starch (corn, maize)
Some Issues:
● High energy input in production, low net yield● Food vs. fuel issues: direct competition with food crops● Requires food crops (starch, e.g. sugarcane, maize, etc ...)● Requires large amounts of arable land● Requires large amounts fresh water● Little potential for increase in production
So what to do?Alternatives are needed!
Cyanobacterial Biofuel Production
![Page 89: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/89.jpg)
Biofuel production from cellulosic biomassBetter and cheaper biomass: non-edible parts of plants:Woodchips (e.g. poplar), fuel crops, grass, corn stover ...
A DIFFICULT AND EXPENSIVE PROCESS (AS YET)
“biomass recalcitrance”: Plant cell walls naturally resist decomposition from microbes and enzymes
Cyanobacterial Biofuel Production
![Page 90: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/90.jpg)
Biofuel production from cellulosic biomassBetter and cheaper biomass: non-edible parts of plants:Woodchips (e.g. poplar), fuel crops, grass, corn stover ...
But considerable potential within the next decade(s)!
Switchgrass (wiki):
Possible Improvements:Manipulate cellusosic content in plantsEnzymatic pretreatment (funghi)Better conversion of 5-carbon sugarsHigher ethanol resistance
Cyanobacterial Biofuel Production
![Page 91: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/91.jpg)
Alternative: Cyanobacteria as a source of biofuels
Cyanobacterial Biofuel Production
![Page 92: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/92.jpg)
Photosynthetic cyanobacteria: An alternative to fossil fuels
Alternative: Cyanobacteria as a source of biofuels
They live from nothing but sun and air
High yield and fast growth rates (up to 6h division time)
Straightforwardly cultivable (algae farms)
Little arable land required
Can be cultivated in seawater An open pond Spirulina farm:
Cyanobacterial Biofuel Production
![Page 93: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/93.jpg)
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Plot courtesy of CYANO BIOFUELS
Cyano Biofuels GmbHDan KramerKarl Ziegler
Biochemistry (HU-Berlin)Wolfgang LockauYvonne Zilliges
Genetics (HU-Berlin)Thomas BoernerJan-Christoph Kehr
Modelling (ITB, HU-Berlin)Ralf SteuerHenning Knoop
Microbiology (U. Giessen)Annegret Wilde
and FRISYS (Freiburg)Wolfgang Hess
Cyanobacterial Biofuel Production
![Page 94: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/94.jpg)
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Plot courtesy of Algenol
Cyanobacterial Biofuel Production
![Page 95: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/95.jpg)
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Plot courtesy of Algenol
Cyanobacterial Biofuel Production
![Page 96: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/96.jpg)
Plot courtesy of http://www.algenolbiofuels.com/
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Cyanobacterial Biofuel Production
![Page 97: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/97.jpg)
The modelling perspective
Progress in strain improvement will depend on the development of theoretical methods that facilitate the elucidation of mechanisms and the identification of genetic targets for modification (*).
(*) from: Exploiting biological complexity for strain improvement through systems biology, G. Stephanopoulos, H. Alper & J. Moxley, Nature Biotechnology 22, 1261 – 1267, 2004
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Cyanobacterial Biofuel Production
![Page 98: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/98.jpg)
What can be, if anything, achieved by mathematical modelling?
1. Network reconstruction and FBA2. Towards largescale kinetic models3. Integrative models: The regulation of metabolism4. Predicting biotechnological modifications
FORSYSPartner: Systems Biology ofCyanobacterial Biofuel Production
Cyanobacterial Biofuel Production
![Page 99: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/99.jpg)
Understanding cellular metabolism1. Network reconstruction and FBA (Henning Knoop)
333 reactions267 metabolitesReconstruction mainly by manual curation
Cyanobacterial Biofuel Production
![Page 100: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/100.jpg)
Understanding cellular metabolism1. Network reconstruction and FBA (Henning Knoop)
333 reactions267 metabolitesReconstruction mainly by manual curation
![Page 101: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/101.jpg)
the end
End of Part II
![Page 102: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/102.jpg)
Hands on examples
Hands-on examples
![Page 103: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/103.jpg)
Hands on examples
1. Numerical Integration in matlab
Hands-on examples
![Page 104: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/104.jpg)
Hands on examples
1. Numerical Integration: Runge-Kutta algorithm
Hands-on examples
![Page 105: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/105.jpg)
Hands on examples
1. Numerical Integration: a simple example
Hands-on examples
then:
![Page 106: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/106.jpg)
Hands on examples
1. Numerical Integration: a simple example
Hands-on examples
then:
![Page 107: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/107.jpg)
Hands on examples
1. Numerical Integration: the Lorenz System
Hands-on examples
![Page 108: Computational Analysis of Metabolic NetworksComputational Analysis of Metabolic Networks Ralf Steuer Humboldt-University Berlin, Germany Institute of Theoretical Biology (ITB) CzechGlobe](https://reader034.vdocuments.site/reader034/viewer/2022042408/5f23b2b9895ec91f6e447504/html5/thumbnails/108.jpg)
Hands on examples
1. Numerical Integration: the Lorenz System
Hands-on examples
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Hands on examples
1. Numerical Integration: the Lorenz System
Hands-on examples
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Hands on examples
1. Numerical Integration: the Lorenz System
Hands-on examples
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Hands on examples
1. Numerical Integration: the Lorenz System
Hands-on examples
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Hands on examples: a metabolic pathway
Hands-on examples
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Hands on examples: a metabolic pathway
Hands-on examples
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Hands on examples: a metabolic pathway
Hands-on examples
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Hands on examples: signal transduction
Hands-on examples
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Hands on examples: a model of the cell cycle
Hands-on examples
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Hands on examples: a model of the cell cycle
Hands-on examples
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Hands on examples: a model of the cell cycle
Hands-on examples
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Hands on examples: a model of the cell cycle
Hands-on examples
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Hands on examples: a model of the cell cycle
Hands-on examples
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Gene expression as a stochastic process
Hands-on examples
Transcription of a mRNADecay of a mRNATranslation of a proteinDecay of a protein
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Gene expression as a stochastic process
Hands-on examples
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Gene expression as a stochastic process
Hands-on examples
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Gene expression as a stochastic process
Hands-on examples
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Gene expression as a stochastic process
Hands-on examples
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Gene expression as a stochastic process
Hands-on examples
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The end
Hands-on examples