Methods in Enzymology

Volume 138

Complex CarbohydratesPart E

Victor Ginsburg

CONTRIBUTORS TO VOLUME 138 . . .'

„ µ,. . . Xi

PREFACE XVi i

VOLUMES IN SERIES Xbi

Section I . Analytical Methods

I: . Isolation and Purification of Glycosphingolipids REIn KANNAGI ,by High-Performance Liquid Chromatography KIYOHIRO WATANABE ,

AND SEN-ITIROH HAKOMORI

:3:`

2 . Microscale Methylation Analysis of Glycolipids STEVEN B . LEVERYUsing Capillary Gas Chromatography-Chemi- AND SEN-ITIROH HAKOMORI

1 3cal Ionization Mass Fragmentography with Se -lected Ion Monitoring

3. : Reductive Cleavage of Permethylated Polysac- GARY R . GRAY

26

charide s

4 . Oligosaccharide Structure by Two-Dimensional THEODORE A . W . KOERNER ,Proton Nuclear Magnetic Resonance Spectros- JAMES H . PRESTEGARD ,copy

AND ROBERT K. Yu

3 8

5 . Direct Chemical Ionization Mass Spectrometry of VERNON N . REINHOLD

59

Carbohydrate s

6 . BioGel P-4 Column Chromatography of Oligosac- AKIRA KOBATA ,charides : Effective Size of Oligosaccharides KATSUKO YAMASHITA ,Expressed in Glucose Units

AND SEHCHI TAKASAKI

8 4

7 . Separation of Benzoylated Oligosaccharides by PETER F . DANIEL

94

Reversed-Phase High-Pressure Liquid Chroma-tography : Application to High-Mannose TypeOligosaccharide s

8 . High-Pressure Liquid Chromatography Analysis M . D . ULLMA Nof Neutral Glycosphingolipids : Perbenzoylated AND R . H . MCCLUER

11 7

Mono-, Di-, Tri-, and Tetraglycosylceramide s

9. Short-Bed, Continuous Development, Thin-Layer WILLIAM W . YOUNG, JR.Chromatography of Glycosphingolipids

AND CHERYL A . BORGMAN

125

10. Analysis of Sialic Acids

ROLAND SCHAUER

132

11 . Resialylated Erythrocytes for Assessment of the JAMES C . PAULSO NSpecificity of Sialyl Oligosaccharide Binding AND GARY N . ROGERS

16 2Protein s

12. Detection of Poly sia]osyl-ContaiBing Glycopro- FREDERIC A . TROY ,teins in Brain Using Prokaryotic-Derived PATRICK C . HALLENBECK ,Probes

RONALD D . McCoy ,AND ERIC R . VIMR

16 9

13. Inositol-Containing Sphingolipids

ROGER A . LAIN EAND THOMAS C .-Y . HSIEH

18 6

14 . Antibodies against Cell Surface Carbohydrates : JOHN L . MAGNANI ,Determination of Antigen Structure

STEVEN L . SPITALNtK ,AND VICTOR GINSBURG

19 5

15 . Immunostaining Free Oligosaccharides Directly JOHN L . MAGNANI

20 8on Thin-Layer Chromatogram s

16 . Preparation of Total Nonacid Glycolipids for KARL-ANDERs KARLSSON

21 2Overlay Analysis of Receptors for Bacteria an dViruses and for Other Studie s

17 . Overlay and Solid-Phase Analysis of Glycolipid KARL-ANDERs KARLSSO NReceptors for Bacteria and Viruses

AND NICKLAS STROMBERG

22 0

18 . Lectin Affinity Chromatography of Glycopeptides ROBERTA K . MERKL EAND RICHARD D . CUMMINGS 23 2

19 . Detection of Glycosaminoglycans with ' uI-La- PHYLLIS M . SAMPSON ,beled Cytochrome C

RALPH HEiMER ,AND ALFRED P . FiSHMAN

26 0

20 . Electrophoretic Separation of Lipopolysaccharide R . C . GOLDMAN AND

26 7Monomers Differing in Polysaccharide Length L . LEIVE

Section H . Preparations

21 . Proteoglycans : Isolation and Purification from Tis- MASAKI YANAGISHITA ,sue Cultures

RONALD J . MIDURA ,AND VINCENT C . HASCALL

279

22 . Isolation of Sialyl Oligosaccharides and Sialyl Oli- JAAKO PARKKINE Ngosaccharide Phosphates from Bovine Colos- AND JUKKA FINNE

28 9trum and Human Urine

23 . Isolation and Purification of Gangliosides from STEPHAN LADISC HPlasma

AND BAIBA GILLARD

30 0

24 . Affinity Purification of Oligosaccharides Using D . ZOPF, K . SCHROER ,Monoclonal Antibodies

G . HANNSON, J . DAKOUR ,AND A . LUNDBLAD

307

25. Fluorescent Gangliosides

SARAH SPIEGEL

31 3

26 . Lysogangliosides : Synthesis and Use in Preparing GÜNTER SCHWARZMAN NLabeled Gangliosides

AND KONRAD SANDHOFF

31 9

27. Chemical Deglycosylation of Glycoproteins

HAKIMUDDIN T . SOJARAND OM P . BAHL

34 1

28. Enzymatic Deglycosylation of Glycoproteins

NAGESWARA T . TIIOTAKURAAND OM P . BAHL

35 0

29. Chemical Synthesis of Oligosaccharides

HAROI .D M . FLOWERS

35 9

30 . Nondegradable Carbohydrate Ligands

Y . C . LEE, C . A . HOPPE ,K . KAWAGUCHI, AND P . LIN 404

31 . Attachment of Oligosaccharide-Asparagine Deriv- ARLENE J . MENCKE ,atives to Proteins : Activation of Asparagine DAVID T . CHEUNG ,with Ninhydrin and Coupling to Protein by Re- AND FINN WOLD

40 9ductive Amination

32 . Covalent Attachment of Oligosaccharide-Aspara- SAU-CHI BETTY YAN

41 3gine Derivatives : Incorporation into Glutamin eResidues with the Enzyme Transglutaminas e

33 . Noncovalent Attachment of Oligosaccharide- VICTOR J . CHEN

41 8Asparagine Derivatives to Protein s

34. Cluster Glycosides

REIKO T . LEE AND Y . C . LEE 42 4

35 . Labeling Glycoconjugates with Hydrazide Re- MEIR WILCHEK AN Dagents

EDWARD A . BAYER

42 9

36 . Biochemical Characterization of Animal Cell Gly- PAMELA STANLEY

443cosylation Mutant s

37 . Isolation of Mutant Chinese Hamster Ovary Cells APRIL R . ROBBIN SDefective in Endocytosis

AND CALVIN F . ROFF

45 8

Section III . Carbohydrate-Binding Protein s

38. Sulfatide-Binding Proteins

DAVID D . ROBERTS

473

39 . Mouse and Rat Monoclonal Antibodies Directed JOHN L . MAGNANI

484against Carbohydrate s

40 . Human Monoclonal Antibodies Directed against STEVEN L . SPITALNIK

49 2Carbohydrate s

41 . Low Molecular Weight Phosphomannosyl Recep- JACK J . DISTLE Rtor from Bovine Testes

AND GEORGE W . JOURDIAN

504

42 . Soluble Lactose-Finding Lectins from Chicken SAMUEL H . BARONDE Sand Rat

AND HAKON LEFFLER

510

43. Xenopus laevis Lectins

SAMUEL H . BARONDESAND MARIE M . ROBERSON

51 6

44 . O-Acetylsialic Acid-Specific Lectin from the Crab M . H . RAVINDRANATHCancer anntenarius

AND JAMES C . PAULSON

520

45 . Properties of a Sialic Acid-Specific Lectin from RONALD L . MILLER

527the Slug Limaxlavus

46. Vicia villosa Lectins

SHERIDA TOLLEFSE NAND ROSALIND KORNFELD

53 6

47. Erythrina Lectins

HALINA LIS AN DNATHAN SHARON

54 4

48. Evonymus europaea Lectin

JERZY PETRYNIA KAND IRWIN J . GOLDSTEIN

55 2

49 . Lectin from Hog Peanut, Amphicarpaea brat- M. MALIARIK, D . ROBERTS ,teata

AND I . J . GOLDSTEIN

56 1

Section IV. Biosynthesi s

50. Glycosyltransferase Assay

Bo E . SAMUELSSON

567

51 . Glycolipids

MANJU BASU, TRIPTI DE ,KAMAL K . DAs ,JOHN W . KYLE ,HUNG-CHE CHON ,ROBERT J . SCHAEPER, AN DSUBHASH BASU

57 5

52 . Acetyl-CoA : a-glucosaminide N-Acetyltransfer- KAREN J . BAM Ease from Rat Liver

AND LEONARD H . Rome

607

53. Sialic Acids : Metabolism of O-Acetyl Groups

ROLAND SCHAUER

61 1

54 . CMP-NeuNAc : Poly-a-2,8-Sialosyl Sialyltrans- RONALD D . McCo yferase in Neural Cell Membranes

AND FREDERIC A . TROY

627

55. Fungal 1,3-ß-Glucan Synthase

ENRICO CABI BAND MOHINDER S . KANG

637

56 . Chitin Synthase from Saccharomyces cerevisiae ENRICO CABIB ,MOHINDER S . KANG ,AND JANICE AU-YOUNG

643

57 . Dextransucrase : A Glucosyltransferase from ROBERT M . MAYER

649Streptococcus sanguis

58 . Glycosylation Inhibitors for N-Linked Glycopro- ALAN D . ELBEIN

66 1teins

59, Transport of Sugar Nucleotides into the Lumen of MARY PEREZVesicles Derived from Rat Liver Rough Endo- AND CARLOS B . HIRSCHBERG 709plasmic Reticulum and Golgi Apparatu s

60 . Plant Cell Wall Polysaccharides That Activate PAUL D . BISHO PNatural Plant Defenses

AND CLARENCE A . RYAN

71 5

Section V. Degradation

61. Enzymatic Diagnosis of Sphingolipidoses

KUNIHIKO SUZUKI

72 7

62. Endo-ß-N-acetylglucosaminidase H from Strepto- ROBERT B . TRIMBLE ,myces plicatus

ROBERT J . TRUMBLY ,

AND FRANK MALEY

76 3

63 . Peptide-N°-(N-acetyl-ß-glucosaminyl) Asparagine ANTHONY L . TARENTINO ANDAmidase and Endo-ß-N-acetylglucosaminidase THOMAS H . PLUMMER, JR.

770from Flauobacterium meningosepticu m

64 . a-Mannosidases I and II from Aspergillus saitoi AKIRA KOBATA AN D

JUNKO AMANO

77 9

65. Polysialic Acid Depolymerase

BARTrOMIEJ KwIATKOWSK IAND STEPHAN STIRM

78 6

66 . Activator Proteins for Lysosomal Glycolipid Hy- ERNST CONZELMAN Ndrolysis

AND KONRAD SANDHOFF

792

67 . Preparation of Keyhole Limpet Oligosaccharyl PREMANAND V . WAGH ,Sulfatase

KALYAN R . ANUMULA ,

AND OM P . BAHL

81 6

AUTHOR INDEX 827

SUBJECT INDEX 861