comparison ns1 with igm captured elisa in dengue

8/6/2019 Comparison NS1 With IgM Captured ELISA in Dengue

1/15

Comparison NS1 with IgM captured

ELISA in dengue Detection

Aji Patriajati

030.05.012


2/15

Dengue Virus

Family : Flaviviridae

Genus : Flavivirus

Four Serotypes :

Den-1

Den-2

Den-3

Den-4


3/15

Virus Morphology

Single-stranded RNA genome surrounded by

an icosahedral nucleocapsid and covered by a

lipid envelope

The genome is composed of three structural

protein genes, encoding the nucleocapsid or

core protein C, a membrane-associated

protein (M), an envelope protein (E) and seven

non-structural (NS) protein genes


4/15

Dengue Virus Polyprotein


5/15

Antibody Responce

Primary Infection

IgM antibodies appear approximately 5 days after onset of symptoms and rise forthe next 1-3 weeks.

IgM antibodies detectable for up to 6 months.

IgG are detectable at approximately 14 days after onset of symptoms and aremaintained for life.

Secondary Infection

Approximately 5% patients do not produce detectable levels of specific IgM.

IgM titer can be slower to rise in secondary infection.

IgG appears approximately 2 days after symptoms appear.

IgG titre significantly higher in secondary infection.


6/15

Dengue fever is an acute viral illness characterized by:

Fever, often with sudden onset;

Severe headache, often described as retro-ocular;

Myalgias and arthralgias that can be very severe;

Nausea and vomiting;

A rash that may present at different stages of the

illness, and whose appearance can be variableit maybe maculopapular, petechial, orerythematous. And

Hemorrhagic manifestations


7/15

NS1

a glycoprotein is detected in high titers in

patients with primary or secondary dengue

infections but its function is unknown

found associated with intracellular organelles

or is transported through the cellular

secretory pathway to the cell surface

found circulating during the acute phase of

the disease in sera from patients


8/15

Dengue Virus Polyprotein


9/15

IgM Spesific dengue

dengue virus-specic IgM as a antibodies

response of dengue virus infection

Produce after 3 days of fever onset usuallyappears on Days 45 of the illness

Detect using ELISA method


10/15

NS1 with IgM ELISA captured

Comparison


11/15


12/15


13/15

Benefit NS1

NS1 AG test kit gives an overall higher sensitivity

rate than the current three established diagnostic

test methods for laboratory diagnosis of acute

dengue infection

NS1 antigen-capture ELISA gave a higher positive

detection within the rst four days of illness

NS1 antigen-capture ELISA has the addedadvantage of continuing to give good detection

rates up to seven days of the illness


14/15

NS1

Still limited by the need for sophisticated

instrumentation

higher technical skill which is normally onlyavailable in large hospital diagnostic

laboratories


15/15

IgM ELISA Captured

The sensitivity rate of IgM assay for early

diagnosis of dengue was poor in the rst three

days of the illness

specic IgM was merely indicative of recent

dengue infection, and not conrmative of

acute dengue infection

comparison ns1 with igm captured elisa in dengue

Documents