combination of natural micro- flora with industrial ... · combination of natural micro-flora with...

COMBINATION OF NATURAL MICRO-FLORA WITH INDUSTRIAL STRAINS IN

BIO REFINERIES

Adj Prof Elias Hakalehto University of Eastern Finland

Adj Prof Elias Hakalehto Beneficial Microbes 2582015 Valencia Spain


MICROBES ARE INVISIBLE TO THE NAKED EYE

BUT WE NOTICE THEIR INFLUENCE ON US


MICROBES LIVE IN THE INTERFACES OF THE THREE PHASES 1 SOLID 2 LIQUID AND 3 GAS PHASE


MICROBES ARE UNIVERSAL

IN NATURE THEY EXIST AS MIXED COMMUNITIES


CONTRARY TO THE ANIMAL SPECIES MICROBIAL STRAINS ARE GLOBALLY DISTRIBUTED THEY LIVE IN COEXISTENCE WITH ANIMAL AND PLANT KINGDOMS AND WITH MAN


IN ORDER TO STUDY THE INTERACTIONS BETWEEN VARIOUS MICROBES WE NEED TOOLS

PMEU versions

Syringes in the PMEU (Portable Microbe Enrichment Unit)

PMEU Scentrion reg

PMEU Spectrion reg

PMEU with Automated Sample Collection System


Hakalehto E 2013 Interactions of Klebsiella sp With other intestinal flora In Pereira LA amp Santos A (eds) Klebsiella infections Epidemiology pathogenesis and clinical outcomes Nova Science Publishers Inc New York USA

EXHAUST GASES OF ONE MICROBIAL CULTURE EXPLOITED BY THE SUBSEQUENT ONES


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MOS1_Cl

MOS2_Cl

MOS3_Cl

ScCell1_Cl

ScCell2_Cl

MOS1_Cl + La

MOS2_Cl + La

MOS3_Cl + La

ScCell1_Cl + La

ScCell2_Cl + La

Hakalehto E amp Haumlnninen O (2012) Can J Microbiol 58 928-931

CARBON DIOXIDE SPEEDS UP THE BACTERIAL GROWTH

Effect of CO2 from preceding PMEU syringe culture on the onset of clostridial growth

Thin lines = C butyricum alone Thick lines = C butyricum connected from L brevis culture

Gaseous CO2 signal initiates growth of butyric-acid-producing Clostridium butyricum in both pure culture and mixed cultures with Lactobacillus brevis



DUALISTIC BALANCE IN THE SMALL INTESTINES

Esherichia coli alone

Ecoli and Klebsiella sp together

Klebsiella alone

GROWTH LEVELS IN PURE CULTURES (AC) AND IN A MIXED CULTURE (B) Klebsiella and E coli GROW UP TO SAME CONCENTRATION AS EACH OF THEM SEPARATELY TOGETHER THEY KEEP THE PH OF DUODENUM AT 6 WITH SIMULTANEOUS EMISSION OF VOLATILES CO2 AND H2

Hakalehto E Humppi T Paakkanen H 2008 Dualistic acidic and neutral glucose fermentation balance in small intestine Simulation in vitro Pathophysiology 15 211-220

A B C


HUMAN GASTROINTESTINAL TRACThellip

hellipIS A BIOREFINERY

Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD

Hakalehto E amp Jaakkola K 2013 Synergistic effect of probiotics and prebiotic flax product on intestinal bacterial balance Poster in 35th ESPEN Congress on Clinical Nutrition and Metabolism September 2013

Leipzig Germany Clinical Nutrition 2013 Vol 32 Supplement 1 S200

The joint effect of a probiotic mixture of Lactobacillus acidophilus Bifidobacterium bifidum and B lactis with prebiotic flax on Escherichia coli growth in the PMEU Note that the logarithmic scale indicates approximately 100-fold restriction of the colibacteria


Patient no Cardia Antrum Pylorus

1 Enterococcus faecalis (2)a

L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus

L salivarius ssp salivarius

2 S salivarius (2)

4 Ssanguinis (2) S salivarius

Ssalivarius

E faecalis

5 S salivarius Str salivarius

L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)

L reuteri Ssalivarius

8 Strsanguinis Lactobacillus

reuteri (2)

9 Lactococcus lactis ssp

lactis (2) S thermophilus

10 S sanguinis S sanguinis S sanguinis

11 Ssalivarius (2) Str salivarius (2) L reuteri (3)

12 S salivarius Strsalivarius (3) Ssalivarius

L reuteri (2)

13 S salivarius (2) S salivarius

Identified LAB (lactic acid bacteria) species enriched from the biopsies obtained from different gastric sites and cultivated with PMEU

Hakalehto E Vilpponen-Salmela T Kinnunen K von Wright A Lactic Acid bacteria enriched from human gastric biopsies ISRN Gastroenterol 2011



Figure 4 (a) Growth of Staphylococcus aureus strain in the TYG medium without peptide additions (b) growth of Staphylococcus aureus strain in the presence of 001 mgmL of M3 sample (c) growth of Staphylococcus aureus strain in the presence of 003 mgmL of M3 sample

Samgina T Yu et al 2012 Journal of Proteome Research American Chemical Society


Study method PMEU Scentrionreg

Hybrid analyzer designed by Adj Prof Elias Hakalehto (Finnoflag Oy Finland) and Dr Heikki Paakkanen (Environics Oy Finland)

16


17 Adj Prof Elias Hakalehto Beneficial Microbes 2582015 Valencia Spain

b)

d)

The influence on growth of Enterobacter cloacae in the PMEU Spectrionreg in TYG medium in presence of different antibiotics a- E cloacae in TYG medium (control) b ndash with penicillin G c ndash with cefuroxime d ndash with netilmicin

a)

c)

18

Hakalehto E 2011 Antibiotic resistance traits of facultative Enterobacter cloacae strain studied with the PMEU (Portable Microbe Enrichment Unit) In Antonio Meacutendez-Vilas (ed) Science against microbial pathogens communicating current research and technological advances Formatex Research Center Badajoz Spain Microbiology Series No3 Vol 2 pp786-796


19

Fast detection of Mycobacterium sp

Hakalehto E Pathophysiology 2013




MICROBES CIRCULATE DIFFERENT ELEMENTS 1 CARBON 2 NITROGEN 3 PHOSPHORUS 4 SULPHUR ETC

FOR A SUSTAINABLE FUTURE WE NEED TO UTILIZE THEIR POTENTIAL TO MAINTAIN THE BALANCES

SOIL MICROBIOTA

VEGETATION FOREST ECOSYSTEM

SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4

Microbial carbon circulation in the Finnish Nature Micro-organisms liberate carbon into the air mostly as CO2 in respiration and fermentation and CH4 in symbiotic fermentation (by acid producing eubacteria and methane producing archae) Any anthropogenic impact on any of the parts of these sequences will eventually effect all the parts and consequently shake the balance

Hakalehto E 2015 Bacteriological indications of human activities in the ecosystems In Armon R amp Haumlnninen O (eds) Environmental indicators Springer Dortdrecht Germany


The Sulphur Cycle


MICROBIAL GAS EMISSIONS REGULATE THEIR COMMUNITIES


Shortening of the onset of Clostridium acetobutylicum strain ATCC 185 growth by carbon dioxide in the PMEU Spectrionreg cultures 4 Then a constant nitrogen (100) flow was in some cases interrupted with pulses of 45 CO2 with 15-30 min duration near the beginning of the cultivation The triggering CO2 impulses produced bacterial growth

Hakalehto E 2015 Enhanced microbial process in the sustainable fuel production In Boehm RF Yang H Yan J (Eds) 2015 Handbook of clean energy systems John Wiley amp Sons Inc Chichester West Sussex UK


SUSTAINABLE BIOECONOMICS BIOTECHNOLOGY IN THE PRODUCTION OF

ENERGY AND CHEMICALS

- Acetone-butanol-fermentation Clostridium acetobutylicum - Products acetone butanol ethanol hydrogen - Butanol is important raw material for chemical industry and a potential liquid fuel - Tested during ABOWE

Source wwwbutanolcom




UNIVERSITY COLLEGE LONDON BIOENGINEERING PILOT PLANT - Prof Jack Drummond vitamins 1930

-studies on antibiotic and enzyme fermentation started about 50 years ago eg by Prof Malcolm D Lilly and Prof Peter Dunnill

- development of bioreactors


EU Baltic Sea Region Projects REMOWE

Regional Mobilizing of Sustainable Waste-to-Energy Production 2009-2012 ABOWE

Implementing Advanced Concepts for Biological Utilization of Waste 2012-2014 Participating countries

Estonia Finland Germany Lithuania Poland Sweden


wwwaboweeu



BIOREFINERIES


Klebsiella sp Escherichia coli Clostridium sp and lactobacilli were studied with different natural microbes as the production organisms

The same cycles that prevail in the environment circulate the substances in the bioreactors

ABOWE MICROBES


We can monitor the microbes and their production of bull biofuels bull chemicals (bulk platform and speciality

chemicals) bull organic fertilizers

by measuring their gas emissions


ABOWE bioreactor (patented)

upper gassing level

lower gassing level

volatile measurement


The ABOWE Pilot A interior


Testing site ZGO Gac Ltd

(Zaklad Gospodarowania Odpadami Sp z oo)

Primary waste to feed in potato waste from a chip factory near Gac

Target product is 23 Butanediol -gt plastics synthetic rubber anti-icing agents cosmetics textileshellip

Also separately collected biowaste has been added to the process


wwwaboweeu

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Run P9 gaseous products

CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)

Biohydrogen production (ppm) and other gases liberated () in ABOWE Pilot A from potato industry waste mixed with sorted restaurant biowastes This pattern of gas formation was measured from a mixed culture of natural microbes and Klebsiella mobilis (HAMBI 1271) Graph by Prof Emilia den Boer Wroclaw University of Technology Poland Hydrogen in red and oxygen in turquoise color


ABOWE Poland liquid products during run 13P (A) and their generation of glucose in different runs (gkg ) (B)

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ethanol acetic acid butyric acid propionic acid 23-butanediol

INDUSTRIAL LEVEL

A

B


ZGO Gac Ltd waste management centre Lower Silesia

SITE

Potato industry waste municipal source-sorted biowaste

BIOMASS

Initial conversion to lactate acetate H2S

PROBLEM

Optimization of the fermentation conditions

SOLUTION

23 butanediol butyrate propionate valerate hydrogen

PRODUCTS


Hagby organic chicken farm from Enkoumlping Sweden Slaughter waste and chicken manure as raw material (dowm)

Joakim Jansson and Elias Hakalehto

peeling apples for sugar source for the

test run


wwwaboweeu

Biogas yield of the different substrates used in the biorefinery pilot runs in Sweden and the residuesdigestate after the first pilot run The y-axis shows the yield in liter biogas per grams of volatile solids (ABOWE Swedish Test Report 2014-2015)

BIOGAS PRODUCTION FROM DIFFERENT SUBSTRATES

Hagby farm (slaughterhouse) Enkoumlping

SITE

Slaughterhouse waste chicken manure saw dust

BIOMASS

Inefficient hydrolysis low soluble carbohydrate

PROBLEM

Conversion to organic acids SOLUTION

Propionate valerate butyrate butanol hydrogen ammonium salts

PRODUCTS


The pioneer of biotechnological NMR studies Prof Reino Laatikainen School of Pharmacy University of Eastern Finland Kuopio Finland He has developed remarkable skills and software for interpreting the NMR graphs


FINNISH LAKE SHORE IN THE MIDDLE OF WINTER (-10 C) BENEATH THE SURFACE MICROBES keep the mud unfrozen by their metabolism (left)



0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol

Proportions of acetate propionate and ethanol in the natural mud sludge (above) and in the industrial waste treatment process of a slaughterhouse (below) Microbial metabolic activities in the latter one produce higher relative amounts of the latter two molecules However proportions to each other remained unchanged Even in the Finnish winter conditions anaerobic metabolism in the mud was high enough to keep it unfrozen under the thick snow belt (approximately 70cm) The mud sludge was slightly basic which also indicated the role of microbiological phenomena in keeping it unfrozen


EQUAL PROPORTION OF ORGANICS IN WINTER LAKE MUD AND SLAUGHTERHOUSE WASTE


Conversion of Lactate into Other Organic Acid in

Slaughterhouse Waste Treatment

Propionibacterium acidipropionici

is accepted as safe production

organism by EFSAn (European

Food Safety Association) It is

used for conserving wheat or

chicken meat for example

httpwwwjournalofdairyscienceorg

articleS0022-0302(98)75663-

2abstract


Hakalehto E 2015 Microbial Food Hygiene Nova Science Publishers Inc New York USA In print

SLAUGHTERHOUSE

WASTE A SLAUGHTERHOUSE

WASTE B

SUPPLEMENT A SUPPLEMENT B

1 000 euro 500 euro

I ENZYMATIC HYDROLYSIS


III THERMAL HYDROLYSIS


BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V

SEPARATION PRODUCT 2

500 euro

PRODUCT 3

PRODUCT 4

1 500 euro PRODUCT 5

500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED

ANIMAL WASTE PROJECT

Euros on daily basis


Pharm Dev Technol 2013 Mar 26 [Epub ahead of print]

Why is mannitol becoming more and more popular as a pharmaceutical excipient in solid

dosage forms

Ohrem HL Schornick E Kalivoda A Ognibene R

Source

Merck KGaA Darmstadt Germany

Abstract

Abstract Various fillersbinders which are applied for the formulation of solid oral dosage forms are

assessed for their benefits and drawbacks including lactose sorbitol mannitol microcrystalline

cellulose and calcium hydrogen phosphate dihydrate A focus of this work was to evaluate the

application of mannitol in comparison to other common fillersbinders as it was observed that this

excipient is gaining more and more attention in pharmaceutical formulation development and

production While one of the main advantages of conventional fillersbinders such as lactose

microcrystalline cellulose and calcium hydrogen phosphate dihydrate is their low price level mannitol

excels regarding its physicochemical characteristics such as a low hygroscopicity a strong inertness

towards both the API and the patients body its good compactibility and the ability to produce extremely

robust tablets Additionally the suitability of mannitol for the emerging formulation technology of orally

disintegrating tablets is pointed out In summary it is emphasized that the selection of the fillerbinder is

highly individual depending for example on the preferred characteristics of the final solid dosage form

the applied API and the available budget However mannitol exhibits many strong advantages which

can be expected to result in a more widespread application in the near future

PMID 23528124

[PubMed - as supplied by publisher]



SAVON SELLU WASTE WATER TREATMENT PLANT


BIOREFINERIES AS A BASIS FOR CIRCULATION ECONOMY

INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE

INDUSTRIAL BIO WASTE

REFINERY SLUDGE

ORGANIC FERTILIZERS COMPOSTING

FUELS FOR MOTORS BIOGASES

(METHANE HYDROGEN HYTANE)


FATE OF CARBON IN INDUSTRIAL SYSTEMS

WASTE WATER

SLUDGE

WASTE PAPER CELLULOSE PAPER COMBUSTION

WOOD WASTE

CO2

PAST IN PAPER AND PULP PRODUCTION


Hakalehto E International Conference of Environmental Indicators Windsor Canada 3-582015


WASTE WATER

SLUDGE

WASTE PAPER

CELLULOSE PAPER COMBUSTION

WOOD WASTE CO2

FUTURE IN FOREST BIOREFINERIES

SIDE STREAM CHEMICALS

ENERGY FUEL BIOCHEMICALS H2 CH4

ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER



Full cell vehicle F-Cell -car which is based on model B Production 200 cars (source Mercedes-Benz)

H2

SAVON SELLU WASTE WATER TREATMENT PLANTrsquoS NATURAL H2 (HYDROGEN) PRODUCTIONDAY BY MICROBES CORRESPONDING TO 10 000 -20 000 KM WITH A FCV (Fuel Cell Vehicle)


wwwaboweeu

Savon Sellu fluting factory Kuopio

SITE

Cellulosic waste BIOMASS

Too high H2S production PROBLEM

Nutrient bed with seed bacteria SOLUTION

23 butanediol ethanol butanol butyratepropionate hydrogen

PRODUCTS


DESIGN OF A NOVEL HYDROGEN PLANT


1 1 2

4 3

Biowaste treatment in the city of Helsinki



Microbiological bioprocess can lower the environmental burden of the combustion of brown coal and other fossilic fuels

Fig National Geographic


BIOREFINERIES are production units based on sustainable principles Sustainability refers to human activities integrated with the balances in the Nature as well as with the circulation of substances In the biorefineries the rdquoworkersrdquo are the microbes and their enzymes Industry Like Naturereg


Thank You



MICROBES ARE INVISIBLE TO THE NAKED EYE

BUT WE NOTICE THEIR INFLUENCE ON US










PMEU versions


PMEU Scentrion reg

PMEU Spectrion reg






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MOS1_Cl

MOS2_Cl

MOS3_Cl

ScCell1_Cl

ScCell2_Cl

MOS1_Cl + La

MOS2_Cl + La

MOS3_Cl + La

ScCell1_Cl + La

ScCell2_Cl + La











Klebsiella alone



A B C




Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD







L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

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INDUSTRIAL LEVEL

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B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You











PMEU versions


PMEU Scentrion reg

PMEU Spectrion reg






0

200

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600

800

1000

1200

1400

1600

0 5 10 15 20 25 30

Rela

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Sample 2

Sample 3

0

02

04

06

08

1

12

0 5 10 15 20 25

Re

lati

ve c

han

ge in

th

e e

lect

ric

char

ges

cau

sed

by

the

gas

em

issi

on

fro

m t

he

cu

ltu

re


MOS1_Cl

MOS2_Cl

MOS3_Cl

ScCell1_Cl

ScCell2_Cl

MOS1_Cl + La

MOS2_Cl + La

MOS3_Cl + La

ScCell1_Cl + La

ScCell2_Cl + La











Klebsiella alone



A B C




Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD







L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

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INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You





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1

12

0 5 10 15 20 25

Re

lati

ve c

han

ge in

th

e e

lect

ric

char

ges

cau

sed

by

the

gas

em

issi

on

fro

m t

he

cu

ltu

re


MOS1_Cl

MOS2_Cl

MOS3_Cl

ScCell1_Cl

ScCell2_Cl

MOS1_Cl + La

MOS2_Cl + La

MOS3_Cl + La

ScCell1_Cl + La

ScCell2_Cl + La











Klebsiella alone



A B C




Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD







L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You






Klebsiella alone



A B C




Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD







L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You





Ventricle

Duodenum

Jejunum

Ileum

Cecum

Large intestines

Distal colon

H2

FOOD







L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You








L salivarius ssp

salivarius (2)

Ls salivarius ssp

salivarius

Str thermophilus


2 S salivarius (2)


Ssalivarius

E faecalis


L salivarius

6

L reuteri Lreuteri

Lcasei 97

7 L reuteri (3)



reuteri (2)






L reuteri (2)











16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You








16



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



b)

d)


a)

c)

18



19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You


19








SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You






SOIL MICROBIOTA


SEDIMENT BACTERIA

PLANKTONIC MICROBES

SWAMPS

ANAEROBIC BACTERIA

PHOTOSYNTHESIS

CO2

CH4




The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You


The Sulphur Cycle























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You























wwwaboweeu



BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You




BIOREFINERIES




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You




ABOWE MICROBES







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You







upper gassing level

lower gassing level











wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You










wwwaboweeu

0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You


0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

10 000

0

5

10

15

20

25

30

12

30

14

00

15

30

17

00

18

30

20

00

21

30

23

00

03

0

20

0

33

0

ppm

time h


CH4 () CO2 () O2 () H2S (ppm) H2 (ppm)




0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



0

1 000

2 000

3 000

4 000

5 000

6 000

7 000

8 000

9 000

90

0

12

00

14

00

16

00

18

00

20

00

22

00

00

0

20

0

40

0

60

0

80

0

10

00

13

00

15

00

17

00

19

00

21

00

23

00

10

0

30

0

50

0

70

0

90

0

11

00

13

00

15

00

mgdm3

time h



INDUSTRIAL LEVEL

A

B



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



SITE


BIOMASS


PROBLEM


SOLUTION


PRODUCTS





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You





test run


wwwaboweeu




SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You





SITE


BIOMASS


PROBLEM



PRODUCTS







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You







0259

0046

0009

acetate

propionate

ethanol

128759

51327

11105

acetate

propionate

ethanol














articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You











articleS0022-0302(98)75663-

2abstract



SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You


SLAUGHTERHOUSE


WASTE B


1 000 euro 500 euro





BIOREACTION A

BIOREACTION B

BIOREACTION C

INOCULUM

II

INOCULUM

IV

INOCULUM

V


500 euro

PRODUCT 3

PRODUCT 4


500 euro

COMPOST

BIOGAS

FRACTIONING

PRODUCT 1

10 000-12 000 euro

PATENTED






dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You




dosage forms


Source


Abstract















PMID 23528124







INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You






INTAKE

HYDROLYSIS

BIO-PROCESS

RECOVERY

BIOREFINERY

SPECIAL CHEMICALS

ALIPHATIC COMPOUNDS

SUGAR ALCOHOLS

ALCOHOLS

ORGANIC ACIDS

ORGANIC MIXED WASTE


REFINERY SLUDGE






WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



WASTE WATER

SLUDGE


WOOD WASTE

CO2





WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



WASTE WATER

SLUDGE

WASTE PAPER


WOOD WASTE CO2




ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER




H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



H2



wwwaboweeu


SITE





PRODUCTS




1 1 2

4 3









Thank You



SITE





PRODUCTS




1 1 2

4 3









Thank You




1 1 2

4 3









Thank You








Thank You


combination of natural micro- flora with industrial ... · combination of natural micro-flora with...

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