colloque enzinov : enzymes innovations industries 27 et 28 ... · world leader in enzymes and...
TRANSCRIPT
LpHera® colloque ENZINOV :
Enzymes Innovations Industries
27 et 28 octobre 2014
Sylvain Laperche –
Strategic Account Manager
Outline
Novozymes in brief
Novozymes sustainability and goals
Starch industry as an example
Alpha amylase history
Refinery processes
How does Novozymes develop new enzyme product
LpHera® plant trial results
Summary
Novozymes in brief
Danish biotech-based company.
World leader in enzymes and microorganisms.
More than 6,000 employees globally.
More than 500 products for many different industries sold in 130 countries.
2013 sales: more than $ 2.1 BUSD.
Novozymes strives for sustainability
Included in DJSI Biotechnology sector since 2000
Sector leader 2000-2007 and 2009-2012
‘Gold Class’ rating in 2010-2013 in Sustainable Asset Management (SAM) Sustainability Yearbook
Novozymes in the Dow Jones sustainability indexes (DJSI):
Enzymes can increase efficiency and yield of a wide range of processes in our society
With enzymes we can “produce more with less” and contribute to the decoupling of economic growth and use of natural resources
Use o
f n
atu
ral
reso
urces
Economic growth
Bioinnovation
Business as usual
Decoupling use of resources from growth
Less chemicals used, significant savings
2013
2015
2030
Million tons CO2
using our solutions instead of traditional processes
Million tons CO2
by switching to more sustainable solutions
Billion tons CO2 using enzymes in industry
52 75 1
Across industries our products help reduce CO2
Cereals:
110 kg CO2 per ton of
bread
Detergent:
100 kg CO2 per ton of laundry
Animal feed:
80 kg CO2 per ton of
feed
Textile:
1100 kg CO2 per ton of
fabric
Agriculture:
15 kg CO2 per ton of
corn
Beverage:
25 kg CO2 per 1000 litre of
beer
Leather:
100 kg CO2 per ton of
hide
Paper making:
150 kg CO2 per ton of
pulp
Vegetable oil:
44 kg CO2 per ton of
oil
Dairy:
230 kg CO2 per ton of mozzarella
Cosmetics:
190 kg CO2 per ton of
fatty acid ester
Margarine:
23 kg CO2 per ton of
hardstock
Alpha-Amylases
Essential enzymes for starch processing
Segment Enzymes
Liquefaction Liquefying a-amylases
Saccharification
Saccharifying amylases Glucoamylase Pullulanase Acidic a-amylase
Speciality syrups and dextrins
b-amylase
CGTase
Maltogenic a-amylase
a-amylase development history
Enzyme
Introduced
Issue addressed
Operating Parameters
Termamyl L (B. licheniformis)
1973 Heat-stability pH 6.2, Ca-addition and inactivation required
Termamyl S (B. stearothermophilus)
1986 (fuel alcohol) Specific activity pH 6.0, Ca-addition and inactivation required
Termamyl LS (mixture of L and S)
1987 Sediments pH 6.0, Ca-addition a nd inactivation required
Termamyl LC (B. lich. hybrid-variant)
1998 Ca-addition and pH pH 5.6, inactivation required
Termamyl SC (B. stearo. variant)
1998 (fuel alcohol) Viscosity pH 5.6, inactivation required
Termamyl Supra (mixture of LC and SC)
1998 Specific activity pH 5.6, inactivation required
Liquozyme X 2002 Product specificity pH 5.6, no inactivation required
Liquozyme Supra 2003 Product specificity, viscosity pH 5.6, no inactivation required
LpHera® 2014 Liquefaction cost savings
pH 4.5 to 5.0
Starch liquefaction processes
Enzyme development Multiple stages
Robustness Testing
PE / Assay
Application Testing
Test Marketing
Low pH Amylase
WO 2013/057141 WO 2013/057143
Protein Engineering (PE) approaches Tailoring nature’s product
Native enzyme Improved acid tolerance
Improved thermostability
Altered specificity
mutations
Engineered amylases Cut further from branches for higher dextrose
Amylopectin
Panose precursors
Traditional Bacterial a-amylase
Panose (DP3)
DP1
SHIFT
Performance Demonstrated improvements at lower pH’s
LpHera® shows excellent DE performance at low pH where conventional alpha-amylases do not.
0
10
20
30
40
50
60
70
80
90
100
110
4 4.2 4.4 4.6 4.8 5 5.2 5.4 5.6 5.8 6
% R
ela
tive D
E p
erfo
rm
an
ce
pH in liquefaction
Novozymes® LpHera
Novozymes Liquozyme® Supra 2.2X
LpHera®
Liquozyme Supra 2.2X
Caustic savings
6% caustic flow rate (liter/min @ 2.38 vs. 1.33)
3
2.5
2
1.5
1
0.5
Cau
sti
c f
low
rate
Liquozyme Supra 2.2X
LpHera®
Plant trial results Syrup conductivity reduction
Sacc sample conductivity (438 vs. 379)
IX feed conductivity (330 vs. 299)
The data indicates that conductivity is lowered, thus reducing IX cost.
480
460
440
420
400
380
360
340
Sacc s
am
ple
co
nd
.
IX
feed
co
nd
.
Liq. Supra 2.2X
LpHera®
400
380
360
340
320
300
280
260
Liq. Supra 2.2X
LpHera®
CH 2 OH
OH
OH
OH
H H
H H
HO
H
O
Dextrose output increased Consistently seen in lab and in full-scale tests
95.1
95.2
95.3
95.4
95.5
95.6
95.7
95.8
95.9
96
% D
P1
00.10.20.30.40.50.60.70.80.9
11.11.21.31.4
% D
P3
Lab scale data. Production scale data might be different
LpHera®
Liquozyme® Supra 2.2X
LpHera® offers a range of benefits From liquefaction and beyond
Drops liquefaction pH to 4.8-5.0, while conventional liquefaction with conventional amylases are done at pH 5.5-5.8.
Saves customer plant chemicals.
Boosts dextrose levels.
Potentially reduces enzyme usages.
Has potential to provide other process savings such as energy, water, and wastewater treatment etc.
Has demonstrated performance in full-scale testing.
LpHera® launches a whole new era of low pH liquefaction.
In summary…
LpHera® alpha amylase:
Contact information: Sylvain Laperche Novozymes Switzerland AG Neumattweg 16 4243 Dittingen Switzerland Phone: +41 617656514 Mobile: +41 794110025 E-mail: [email protected] Web: www.novozymes.com