college of the canyons biology 107 practice lab final updated 2011 1.press f5 to switch to slide...
TRANSCRIPT
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College of the CanyonsBiology 107 Practice Lab Final
Updated 2011
1. Press F5 to switch to Slide Show2. Click your mouse or hit space bar to
proceed to next slide
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Metric Practice
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Prefix Symbol Power Number Equivalent
Giga- G 109 1,000,000,000 Billion
Mega- M 106 1,000,000 Million
kilo- k 103 1,000 Thousand
hecto- h 102 100 Hundred
deka- dk 101 10 Ten
Base Unit 100 1 One
deci- d 10-1 0.1(1/10)
Tenth
centi- c 10-2 0.01(1/100)
Hundredth
milli- m 10-3 0.001(1/1000)
Thousandth
micro- m 10-6 0.000001(1/1,000,000)
Millionth
nano- n 10-9 0.000000001(1/1,000,000,000)
Billionth
Metric System Help Sheet
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The metric system is a base 10 system, that is you can change between units by multiplying and dividing by powers of ten.
For example:1 km = 1000 m = 103 m1 mg = g = 0.000001 g = 10-6 g1 L = 100 cL = 102 cL
0000001
1
,,
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Sample Metric Conversion Problems
1 dg = ____ g0.1
4 mL = ____ cL
4 nm = ____ mm
6 m = ____ mm
7 g = ____ kg
3 g = ________ mg
6000
0.4 0.007
0.000004 3,000,000
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Looking at Life
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Ocular Lens Objective Lens
Coarse adjustment knob
Fine adjustment
knobCondenser knob
Name the parts of the microscope
Condenser
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Name this tissue
100X
Bone
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Name this tissue
Complete cross sectionClose up
Ranunculus Root
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Which of these objectives will have the largest working distance?
The 4X objective (shortest)
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What is the total magnification when thismicroscope is set as shown?
4 (objective) x 10 (ocular) = 40X
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Biological Molecules
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A B
What molecule is detected by the Iodine Test?
Tube A
Which tube is positive for the Iodine test?
–Detects Starch
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What molecule is detected by the Biuret Test?
Tube A
Which tube is positive for the Biruet test?
–Detects Protein
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A B C
What does the Benedict’s test detect?
Detects Reducing Sugar
Which of the tubes below is a strong positive for the Benedict’s test? Tube C
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Spot A Spot B
What does the Sudan test detect?
Detects lipids
Which spot is positive for the Sudan test?
Spot B
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Enzymes in Action
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On the diagram below, identify:
Substrate
Enzyme
Active site
–active site –substrate–the enzyme
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Complete the following Fermentation Reaction
Sugar + enzymes CO2 + Ethanol+ ATP+ heat
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LactoseFructose
Yeast Fermentation
Name the glassware
Fermentation Tubes (or J Tubes)
What are the air bubbles made up of?
Carbon Dioxide (C02)
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In the enzyme lab, which enzyme was tested for its abilityto cleave sucrose?
Enzyme = Sucrase (or Invertase)
Test = Benedict’s
Which biochemical test was used to determine if this enzyme was working?
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Below are example results where enzyme samples weremixed with different pH buffers. According to these results, which buffer is optimal for the enzyme?
pH 2 pH 4 pH 6 pH 8
pH 2 (red = most activity)
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Diffusion/Osmosisand
Probing the Cell
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Name these instruments
Centrifuges
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supernatant
green layer of pellet
beige layer of pellet
white layer of pellet
Which organelles are found in the supernatant and colored pellet layers of a centrifuged pea solution?
mitochondria
chloroplasts
nuclei
starch grains
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Which test tube shows the presence of mitochondria?
A B C
Tube B
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Which substance entered the bag by diffusion?
Iodine
What did the iodine react with inside the bag?
Starch
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Photosynthesis
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Spectroscope
Name the instrument
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What gas is released by this Elodea plant as it performs photosynthesis?
What solution acts asthe carbon dioxidesource for this Elodeaplant?
Oxygen
Sodium bicarbonate
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What is the function of the copper sulfate solution (blue) in the photosynthesis setup shown below?
Filters out heat from light bulb
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What is this experimental setup used for?
What solvent wasused?
Paper Chromatography
acetone
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How is the Ratio of Fronts (RF value) calculatedfor pigments separated by paper chromatography?
Rf =
= xy=
x (distance of pigment)
y (distance of solvent)
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Spectrophotometer
Name the instrument
What does it measure?
Absorbance and Transmittance of light
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The absorption spectrum for chlorophyll is shown below.According to this chart, what colors of light areabsorbed by green plants?
Predominantly blue/violet and red
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DNA Extraction LabFrom Fruit Fly & Onion
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Identify this organism and the tissue that you dissected from it.
Fruit fly (Drosophila) larvaeSalivary glands
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What is this structure?
Drosophila polytene chromosome
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What reagent was added to the onion DNA tube to make the DNA precipitate (turn solid) ?
Cold 95% Ethanol
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Micropipetting/Electrophoresis
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What is the substance that gels are made of?
Agarose
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wells
The gel below shows DNA cut by the restrictionenzyme EcoRI. How many times did EcoRI cutthe DNA sample in Lane 2?
Lane 4 = 3 cuts
Lane 2 = 0 cuts
–In Lane 4?
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Electrophoresis Setup
Power unit Gelbox
Name each piece of equipment:
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What is the name of this instrument?
micropipette
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If this is a p200 micropipette, what volume is it set to?
50 microliters (ul)
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Bacterial Transformation andProtein Purification Lab
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What is the function of the three genes found on thepGLO plasmid?
AraC:
GFP:
AmpR =resistance to ampicillin (selection marker)
activates GFP. Activated by arabinose
green fluorescent protein
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What two treatments are needed to get bacteria to absorb plasmid DNA (aka transformation)?
1. Incubate in cold CaCl2
2. Heat shock at 420C
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What is the transformation efficiency for the P+ plate shown below if 2 ug of DNA were used in the transformation experiment?
TE = 37 colonies 2ug DNA
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What is the name for this column?
What is the name of this protein?
What type of light makesthese colonies “glow”?
Hydrophobic interaction column
Ultra violet
GFP, green fluorescent protein
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Embryology
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Name the layers
1
2
3
Mesoderm
Endoderm
Ectoderm
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Name the developmental stage
Early Cleavage
100X
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Name the developmental stageLate Cleavage
100X
Name the developmental stageBlastula
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Name the developmental stage
Unfertilized Egg
200X
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Name this developmental stage
100X
Gastrula
Name this structure
Archenteron
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Name this organism
24-33 hr Chicken Embryo
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Pig Anatomy & Histology
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Oral CavityName the following structures:
Hard PalateSoft Palate
Epiglottis
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Abdominal Cavity
Name the organs:
Liver
Gallbladder Stomach
Spleen
Small Intestine
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Female Urogenital Tract
Ovary
Uterine Horns
Uterus/Vagina
Bladder
Name the following organs:
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Male Urogenital Tract
Name the organs:
Bladder
Penis
Epididymis
Scrotum
Teste
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Thoracic CavityName the organs:
Larynx
Carotid Arteries Jugular Veins
Thyroid
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Heart
Pulmonary Trunk
Atrium
Ventricle
Inferior Vena Cava
Name the organs:
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HISTOLOGY
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What tissue is this?Stomach
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What tissue is this?
Smooth Muscle
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What tissue is this?Frog Skin