College of Engineering, Computing and Applied Science Mark Alan Blenner PERSONAL DATA McQueen-Quattlebaum Associate Professor Chemical & Biomolecular Engineering Clemson University Clemson, SC 29634 864-656-0290 May 20, 1982 Queens, New York USA EDUCATION Ph.D., Columbia University, 2009, Chemical Engineering M.S., Columbia University, 2006, Chemical Engineering B.S., Manhattan College, 2004, Chemical Engineering PROFESSIONAL EXPERIENCE

NASA 2019-Present, Visiting Scientist, Synthetic Biology Group, Bioengineering Division

Clemson University 2019-Present, Beckman Scholars Mentor 2018-Present, Director, Molecular Engineering for Sustainability & Health GAANN 2018-Present, McQueen-Quattlebaum Associate Professor 2018-Present, Associate Professor (Courtesy), Biological Sciences 2018-Present, Associate Professor, Chemical & Biological Engineering 2018, Assistant Professor (Courtesy), Biological Sciences 2016-2018, CECAS Dean’s Assistant Professor 2016-Present, Faculty Fellow, CU School of Health Research 2014-Present, Coordinator, Biomanufacturing & Bioproducts Consortium 2012-2018, Assistant Professor, Chemical & Biomolecular Engineering

Harvard Medical School 2011-2012, Research Fellow, Biological Chemistry & Molecular Pharmacology 2009-2011, Research Fellow, Pathology

Children’s Hospital Boston 2009-2012, Research Fellow, Immune Disease Institute

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Coca Cola, North America 2002, Intern, Water-Energy-Solid Waste Division

HONORS AND AWARDS

Presidential Early Career Award for Scientists & Engineers (PECASE) (2019) NIH Outstanding Investigator Award (2019) Outstanding Junior Researcher of the Year, Clemson University (2019) Outstanding Junior Researcher of the Year, CECAS (2019) McQueen-Quattlebaum Junior Professorship (2018-2023) Camille Dreyfus Teacher-Scholar University Nominee (2018) CECAS Dean’s Professorship Award (2016-2019)

NASA Early Career Faculty Award (2015) Air Force Office of Scientific Research, Young Investigator Award (2015) Ruth L. Kirschstein National Research Service Award, NIH (2011-2012) Charles Lapple Fellowship, Columbia University (2007-2009) Draddy Medal for Excellence in Engineering, Manhattan College (2004) Omega Chi Epsilon Award, Manhattan College (2004) Sigma Xi (2004) Epsilon Sigma Pi (2003) Tau Beta Pi (2002) PUBLICATIONS (undergraduate, *corresponding author, ‡equal contribution).

Books and Monographs (1)

1. Blenner, M.* "Enzymatic Deconstruction of Lignin for Biofuels" in Advances in Enzymatic Conversion of Biomass to Biofuels, 1st Edition (2015), Future Medicine Ltd, Montreal Canada.

Refereed Journal Publications (25)

2. Schwartz, C., Cheng, JF., Evans, R., Schwartz, CA., Wagner, JM., Anglin, S., Beitz, A., Pan, W., Lonardi, S., Blenner, M., Alper, H., Yoshikuni, Y., Wheeldon, I.* “Validating genome-wide CRISPR-Cas9 function in the non-conventional yeast Yarrowia lipolytica” Metabolic Engineering 55:9 102-110 (2019).

3. Spagnuolo, M., Yaguchi, A., Blenner, M.* “Engineering of oleaginous yeast for biofuels production: recent advances and future directions.” Current Opinion in Biotechnology 57:73-81 (2019).

4. Gao, D., Smith, S., Rodriguez, G., Shabbir Hussain, M., Blenner, M.* “Dual CRISPR-

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Cas9 Cleavage in Yarrowia lipolytica Leads to Efficient Gene Excision and Targeted Integration”, Biotechnology Journal 13:9 1-8 (2018) doi: 10.1002/biot.201700590. 5. Spagnuolo, M., Shabbir Hussain M., Gambill L., Blenner, M.*, “Alternative Substrate Metabolism in Yarrowia lipolytica”, Frontiers in Microbiology 9:1077 (2018). 6. Brabender, M., Shabbir Hussain, M., Rodriguez, G., Blenner, M.* “Urea and Urine are Efficient Nitrogen Sources for Growth of Yarrowia lipolytica”, Applied Microbiology and Biotechnology 102(5):2313-2322 (2018) DOI: 10.1007/s00253-018-8769-z 7. Yaguchi, A., Spagnuolo, M., Blenner, M*. “Engineering Yeast for Utilization of Alternative Feedstocks” Current Opinion in Biotechnology 53:122-129 (2018). 8. Yaguchi, A., Robinson A., Mihealsick E., Blenner, M.* “Metabolism of Aromatic Compounds by Trichosporon oleaginosus While Remaining Oleaginous”, Microbial Cell Factories 16:206 (2017) DOI: 10.1186/s12934-017-0820-8 9. Dasetty, S., Blenner, M.*, Sarupria, S.* “Engineering Lipases: Walking the Fine Line Between Activity and Stability” Materials Research Express – Emerging Investigators Awards Series 4:114008 (2017) DOI: 10.1088/2053-1591/aa9946 10. Shabbir Hussain, M., Wheeldon, I., Blenner, M.* “A Strong Hybrid Fatty Acid Inducible Transcriptional Sensor Built from Yarrowia lipolytica Upstream Activating and Regulatory Sequences”, Biotechnology Journal 12(1):1-11 (2017). DOI: 10.1002/biot.201700248. Research is featured on the inside cover. 11. Bulutoglu, B., Dooley, K., Szilvay, G., Blenner M., and Banta, S.* “Catch & Release: Fishing for target proteins with an evolved β-roll peptide exhibiting allosteric regulation”, ACS Synthetic Biology 6(9):1732-1741 (2017). DOI: 10.1021/acssynbio.7b00089 12. Yaguchi, A.‡, Rives, D.‡, Blenner, M.* “The New Kids on the Block: Emerging Oleaginous Yeast of Biotechnological Importance”, AIMS Microbiology 3(2):186-206 (2017). Top 4 most read in 2017. 13. Schwartz, C., Shabbir-Hussain, M., Frogue, K., Blenner, M., Wheeldon, I.* “Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica”, ACS Synthetic Biology 6(3):402-409 (2017). ISI Highly Cited. 14. Shabbir Hussain, M.‡, Rodriguez, G.‡, Gao. D., Spagnuolo M., Gambill, L., Blenner, M.* “Recent Advances in Bioengineering of the Oleaginous Yeast Yarrowia lipolytica”, AIMS Bioengineering 3(4):493-514 (2016). 15. Rodriguez, G.‡, Shabbir Hussain, M.‡, Gambill L., Gao, D., Yaguchi, A., Blenner, M.* “Engineering Xylose Utilization in Yarrowia lipolytica by Understanding its Cryptic Xylose Pathway”, Biotechnology for Biofuels 9:149 (2016). 16. Schwartz, C., Shabbir-Hussain, M., Blenner, M., Wheeldon, I.* “Synthetic RNA

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Polymerase III Promoters Facilitate High Efficiency CRISPR-Cas9 Mediated Genome Editing in Yarrowia lipolytica”, ACS Synthetic Biology 5(4):356-359. (2016). Paper is most read during past 12 months in 2016-2017. ISI Highly Cited. 17. Shabbir Hussain, M., Gambill L., Smith, S., Blenner, M.* “Engineering Promoter Architecture in Oleaginous Yeast Yarrowia lipolytica”, ACS Synthetic Biology, 5(3):213-223. (2016). Research is featured on the cover of the March 2016 issue. 18. Wilson, A., Blenner, M., Guiseppi-Elie, A.* “Polyplex Formation Influences Release Mechanism of Mono- and Di-Valent Ions from Phosphorylcholine Group Bearing Hydrogels”, Polymers, 6, 2451-2472 (2014). 19. Nedumpully-Govindan, P., Lin, L., Alexov, E., Blenner, M., Ding, F*. “Structural and Energetic Determinants of Tyrosylprotein Sulfotransferase Specificity”. Bioinformatics, 15(30):2302-2309 (2014). 20. Blenner, M., Dong, X., Springer, T*. “The structural basis of regulation of von Willebrand Factor binding to glycoprotein Ib”, J. Biological Chemistry, 289:5565-5579 (2014). 21. Shur, O., Dooley, K., Blenner, M., Baltimore, M., Banta, S*. “A designed, phase changing RTX-based peptide for efficient bioseparations”, Biotechniques, 54(4):197-206 (2013). Prior to Clemson

22. Banta, S.*, Wheeldon, I., Blenner, M. “Protein engineering in the development of functional hydrogels” Annual Reviews in Biomedical Engineering, 12, 167-186 (2010)

23. Blenner, M., Shur, O., Szilvay, G., Cropeck, D., Banta, S*. (2010), “Calcium induced folding of a Repeat in Toxin (RTX)- Domain Via C-Terminal Entropic Stabilization” Journal of Molecular Biology, 400, 244-256 (2010).

24. Szilvay, G., Blenner, M., Cropeck, D., Banta, S*. “A FRET-based Method for Probing the Conformational Behavior of an Intrinsically Disordered Repeat Domain from Bordetella pertussis Adenylate Cyclase” Biochemistry, 48, 11273-11282 (2009).

25. Blenner, M., Banta, S*. “Characterization of the 4D5Flu single chain antibody with a stimulus-responsive elastin-like peptide linker: A potential reporter of peptide linker conformation” Protein Science, 17, 527-536 (2008).

26. Chockalingam, K., Blenner, M., Banta, S*. “Design and application of stimulus responsive peptide systems” Protein Engineering Design and Selection, 20, 155-61 (2007).

Patents & Intellectual Property

1. Banta, S*., Blenner, M., Wheeldon, I., Dooley, K. “Leucine Beta Roll Domains And Uses Thereof” US 9,127,267 B2 (September 2015)

Non-Refereed Journal Publications

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1. Blenner, M. “Microbes might be key to a mars mission” Scientific American Blog, 2019. https://blogs.scientificamerican.com/observations/microbes-might-be-key-to-a-mars-mission/

2. Blenner, M. “Influence of epigenetics on bioprocessing” Chemical Engineering Progress, 9:72-76 (2018).

3. Schwartz, C., Cheng, JF., Evans, R., Schwartz, CA., Wagner, JM., Anglin, S., Beitz, A., Pan, W., Lonardi, S., Blenner, M., Alper, H., Yoshikuni, Y., Wheeldon, I.* “Validating genome-wide CRISPR-Cas9 function in the non-conventional yeast Yarrowia lipolytica” BioRxiv http://dx.doi.org/10.1101/358630 (2018).

Publications Submitted

1. Manglass, L.; Wintenberg, M.; Blenner, M.; Martinez, N. "The influence of citrate on plutonium-239 concentration in E. coli and P. putida grown in liquid cultures" Health Physics Journal (In Review). 2. Molin, W., Yaguchi, A., Blenner, M., Saski, C. “The eccDNA Replicon: A self-replicating, extra-nuclear vehicle driving gene amplification and rapid adaptive evolution” Science Advances (In Revision). 3. Ganesen, V. ‡, Spagnuolo, M. ‡, Agrawal, A. ‡, Smith, S., Gao, D., Blenner, M.* “An expanding toolkit for metabolic engineering of Yarrowia lipolytica” Microbial Cell Factories (In Revision).

4. Blenner, M.* “Gene excision by dual-guide CRISPR-Cas9” Methods in Molecular Biology, Yarrowia lipolytica (In Review).

5. Blenner, M.* “Simultaneous gene excision and integration with dual-guide CRISPR-Cas9” Methods in Molecular Biology, Yarrowia lipolytica (In Review).

6. Yaguchi, A., Lee, S., Frankaszek, N., O’Neill, K., Boundy-Mills, K., Blenner, M.*. “Identification of aromatic metabolizing oleaginous yeast”. Journal of Industrial Microbiology & Biotechnology (In Preparation).

PRESENTATIONS (162 Total; *speaker; #invited talk (30); ‡program review; undergraduate).

SPONSORED RESEARCH Total extramural funding of approximately $8.60 million ($6.7 million in force; $7.18

million dollars to MAB), derived from NSF, AFOSR, NASA, DTRA, Dept of Ed, NIH, USDA, NOAA, Industrial Sponsors.

ACTIVE FUNDING “Establishing Protective Biomanufacturing Processes for Human Systems in Remove Environments”, DTRA through Rhodium Scientific, co-Principal Investigator, $150,000 ($75,000), (2019-2021).

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“Enabling Terrestrial Omega-3 Production from Local Natural Resources”, NOAA – New Aquaculture Opportunities, Principal Investigator, $150,000 ($100,000 federal), (2019-2021). “Enabling Plant Natural Product Biosynthesis by Debugging Heterologous Protein Expression in Yeast”, NIH R35 MIRA ESI, Principal Investigator, $1,767,240 ($1,767,240), (2019-2024). “Increasing Genomic Stability and Antibody Titer with a Mammalian Artificial Chromosome”, AMBIC Project, co-Principal Investigator, $75,000 ($37,500), (2019). “GAANN: Molecular Engineering for Sustainability and Health” Department of Education, Principal Investigator, $936,950 ($749,560 federal), (2018-2021). P200A180076. “Understanding and Controlling ER Stress in Highly Producing CHO Cells” AMBIC Project, Principal Investigator, $105,000 ($105,000), (2018-2019). “Collaborative Research: Controlling Cellular Physiology and Enzyme Localization for Enhanced Oleochemical Biosynthesis in Yeast”, NSF CBET Cellular & Biochemical Engineering, Principal Investigator, $355,277 ($355,277), (2017-2020). Award CBET:1706134.

“Discriminatory Transcriptional Response of Environmental Microorganisms to Low-Dose Ionizing Radiation” Defense Threat Reduction Agency (DTRA), Co-Principal Investigator $1,643,585 ($873,084), (2017-2022).

“Predictive Structure-Function Relationships for Enzymes Immobilized on Complex Surfaces”, Defense Threat Reduction Agency (DTRA), Principal Investigator, $823,899 ($487,983), (2016-2021). HDTRA1-16-1-0023.

“Synthetic Biology for Recycling Human Waste into Nutraceuticals, and Materials: Closing the Loop for Long-Term Space Travel”, NASA Early Career Faculty Award, Principal Investigator, $1,004,144 ($1,004,144), (2015-2022). NNX15AU46G

COMPLETED FUNDING “Palmetto Academy: Synthetic Biology in Space”, SC Space Grant Consortium, Principal Investigator, $36,000 ($18,000 external), (2019-2020). “Optimization and Initial Bioprocess Scale Up of Omega-3 Production from Rendered Fat”, Animal Coproducts Research & Education Center, Principal Investigator, $38,500 ($38,500), (2016-2019). “A Proteomics View of the Temporal Evolution of ER-Stress in Highly Producing CHO Cells” DRP SC INBRE, Principal Investigator, $75,000 ($75,000), (2018-2019). “Generation of Complex-I Deficient Yarrowia lipolytica”, Perlara Inc., Principal Investigator, $5,000 ($5,000), (2019). “CRISPR-Enabled Genome-Wide Screening of the Oleaginous Yeast Yarrowia lipolytica” DOE Joint Genome Institute: Community Science Program in Synthetic Biology, Co-Principal Investigator, 1.5 Mb DNA Synthesis ($0 real; ~$200,000 value) (2016-2019) DOE-DE-AC02-05CH11231 “Omega-3 and Fatty Acid Production from Sweet Sorghum” USDA/DOE Southeastern Regional Sun Grant, Principal Investigator, $197,999 ($197,999), (2016-2019). 2014-38502-22588

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“Enhanced Production of Advanced Biofuels through Model Guided Synthetic Biology”, NSF CBET Energy for Sustainability, Principal Investigator, $336,051 ($336,051), (2015-2019). Award CBET:1437836 “Determining the Role of Chromatin Structure on the Activity of CRISPR-Cas9” SC INBRE Bioinformatics Pilot Project, Principal Investigator, $10,000 ($10,000), (2017-2018).

“Understanding the Influence of Kinetics and Structure in CRISPR-Cas9 Cutting of Non-Conventional Yeast” SC EPSCoR/IDeA, Principal Investigator $10,000 ($10,000), (2017-2018).

“Engineering Robust Enzyme Activity Through Fundamental Studies of Extremophile Enzymes”, AFOSR YIP, Principal Investigator, $359,966 ($359,966), (2015-2019). Award FA9550-15-1-0163 “REU Site: Interfaces and Surfaces” NSF DMR, Senior Investigator, $330,000 ($10,890) (2015-2018) Award DMR: 1460863 “Refactoring Lignin Degradation and Utilization Pathways for Bioenergy Applications” DOE Joint Genome Institute: Community Science Program in Synthetic Biology, Principal Investigator, 200kb DNA Synthesis ($0 real; ~$90,000 value) (2014-2018) DOE-DE-AC02-05CH11231

“Intracellular Localization of Biosynthetic Pathways for Conversion of Lipids to Dicarboxylic Acids in Oleaginous Yeast Yarrowia lipolytica”, NSF CBET Biotechnology, Biochemical, & Biomass Engineering, Principal Investigator, $313,127 ($313,127), (2014-2018). Award CBET:1403099 “Fluorescence Activated Cell Sorter”, Defense University Research Instrumentation Program (DURIP), Principal Investigator, $175,100 ($175,100), (2016-2017). “Biocatalytic Conversion of Rendered Animal Fats to Value Added Products Including Omega-3 Fatty Acids”, Animal Coproducts Research & Education Center, Principal Investigator, $33,000 ($33,000), (2015-2016). “BioNanomanufacturing of Carbide Aerogels”, SC Space Grant Consortium, Co-Principal Investigator, $50,000 ($12,500), (2015-2016). “Use of High-Throughput Sequencing to Understand Protein Evolution”, SC INBRE Bioinformatics Pilot Project, Principal Investigator, $10,000 ($10,000), (2014-2016). Award NIH:P20GM103499 “Research Opportunities for Women in Biomolecular Engineering” SC EPSCoR Track III, Principal Investigator, $10,000 ($10,000), (2016).

“Palmetto Academy: Synthetic Biology Enabled Recycling of Waste for Life Support”, SC Space Grant Consortium, Principal Investigator, $36,000 ($36,000), (2016). “Biocatalytic Conversion of Rendered Animal Fats to Value Added Products Including Omega-3 Fatty Acids”, Animal Coproducts Research & Education Center, Principal Investigator, $27,500 ($27,500), (2014-2015).

Prior to Clemson

“Enabling Structural Studies of Force Activated Adhesion Complexes”, National Institutes of Health, Principal Investigator, $126,767 ($83,321), (2011-2012)

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“A Novel Extended-State Complex between VWF A1 and Platelet GPIb”, American Heart Association, Principal Investigator, $83,000, ($20,500), (2010).

“Computation Models for Reductive Dechlorination of Dioxins and Dibenzofurans”, Howard Hughes Medical Institute, Investigator, $3000 ($3000), (2002).

Additional Research Funding CU FELLOWS: Polymersome Delivery of CRISPR-based Therapies for Brain Diseases:

Towards a Center for Nanobiopharmaceutical Systems, Principal Investigator, $140,300, (2018-2020). Open Access Funding Initiative for Publication in Biotechnology for Biofuels, Principal Investigator, $1,041.25 ($1,041.25), (2016).

“Integration of Computation, Evolutionary Design, and Genomics for High-Throughput Engineering of Protein-Ligand Interactions”, CoES TIGER Grant Program, Principal Investigator, $18,000 ($18,000), (2014).

“Getting to Mars with Microbes”, Clemson Creative Inquiry, Principal Investigator, $2,600 ($2,000), (2017-Current). “Yeast Sustainable Chemical Production in Engineered Microorganisms”, Clemson Creative Inquiry, Principal Investigator, $16,690 ($16,690), (2015-Current).

“Engineering Protein Post-Translational Modification for Therapeutics”, Clemson Creative Inquiry, Principal Investigator, $13,700 ($13,700), (2013-Current).

“Sustainable Chemical Production in Engineered Microorganisms”, Clemson Creative Inquiry, Principal Investigator, $32,090 ($32,090), (2013-Current).

“Getting to Mars with Microbes”, Clemson Creative Inquiry, Principal Investigator, $2,400 ($2,400), (2017-Current).

PROFESSIONAL ACTIVITIES

Professional Leadership

American Chemical Society, Program Chair, Biochemical Technology Division (2020)

Space Travel: Adaptive Research and Technologies from Biological and Chemical Engineering (STAR Tech), AIChE Conference, Organizing Committee (2019, 2018)

American Chemical Society, Area Coordinator, Biochemical Technology Division (2019, 2018, 2016)

American Chemical Society, Executive Committee – Technology Coordinator, Biochemical Technology Division (2014-Present)

Editorship

Biotechnology Notes, Founding Editorial Board (2019-Present)

Metabolic Engineering Communications, Editorial Board (2018-Present)

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Biochemical Engineering Journal, Editorial Board (2017-Present)

Microbial Cell Factories, Editorial Board (2017-Present)

Invited Editor for Current Opinion in Biotechnology – Food Biotechnology 2020 (2018 – Present)

Bioengineering MDPI, Associate Editor (2017-Present)

Invited Co-Editor of “Methods in Molecular Biology: Yarrowia lipolytica” (2017-Present).

AIMS Bioengineering, Editorial Board (2016-Present)

Guest Editor for Bioengineering MDPI: Special Issue on Metabolic Engineering (2015)

Professional Society Meeting Organization

American Chemical Society, Program Chair, Biochemical Technology Division (2020) “Metabolic Engineering – Pathway Engineering for Biochemical Production”, American

Institute of Chemical Engineers Annual Meeting, Session Chair, (2019) “Metabolic Engineering – Non-Model Organisms”, American Institute of Chemical Engineers

Annual Meeting, Session Chair, (2019) “Frontiers of BIOT”, American Chemical Society Annual Meeting, Area Coordinator, (2019) “Advances in Metabolic Engineering”, American Institute of Chemical Engineers Annual

Meeting, Session Chair, (2018) “Biochemical Technology Division Poster Session”, American Chemical Society Annual

Meeting, Area Coordinator, (2018) “Detecting Epigenetic Modifications”, International Conference on Epigenetics and

Bioengineering, Session Chair, (2017) “CRISPR-Cas9 in Yeast: Roundtable Discussion”, International Conference on CRISPR

Technologies, Host, (2017) “Protein Structure, Function, and Stability I & II”, American Institute of Chemical Engineers

Annual Meeting, Session Chair, (2017) “Journey to Mars”, American Chemical Society – Division of Polymers Fall Annual Meeting,

Session Chair (2017) “Metabolic Engineering of Fuels and Chemicals”, Society of Industrial Microbiology &

Biotechnology Annual Meeting, Session Chair (2017) “Synthesis in Space”, American Chemical Society – Division of Biochemical Technology

Annual Meeting, Session Chair (2017) “Biobased Fuels and Chemicals II: Recalcitrant Feedstocks”, American Institute of Chemical

Engineers Annual Meeting, Session Chair, (2016) “Biobased Fuels and Chemicals II: Beyond Glucose”, American Institute of Chemical

Engineers Annual Meeting, Session Chair, (2015)

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“Upstream Processes: Biocatalysis”, American Chemical Society – Division of Biochemical Technology Annual Meeting, Session Chair (2015)

“Protein Engineering I – Combinatorial Techniques”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2014)

“Advances in Protein Post-Translational Modification”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2014)

“Biobased Fuels and Chemicals I: Pathway Engineering”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2014)

“Upstream Processes: General Topics”, American Chemical Society – Division of Biochemical Technology Annual Meeting, Session Chair (2014)

“Protein Engineering IV – Therapeutics”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2013)

“Protein Structure, Function, and Stability”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2013)

“Protein Expression & Post-Translational Modification”, American Institute of Chemical Engineers Annual Meeting, Session Chair, (2012)

GRADUATE STUDENT ADVISING Doctoral Graduates

Shabbir-Hussain, Murtaza, “Expanding the Genetic Toolbox to Improve Metabolic engineering in the Industrial Oleaginous Yeast, Yarrowia lipolytica” (Ph.D. Dec. 2017). Currently at Zymergen. Wilson, A. Nolan, “Drug Delivery with Feedback Control in Bioresponsive Hydrogels” (Ph.D. May 2014) Co-advised with Anthony Guiseppi-Elie. Currently at NREL.

Current Graduate Advising Agrawal, Ayushi, “Engineering Natural Product Biosynthesis in Oleaginous Yeast” (Ph.D. Expected 2024) Ganesen, Vijay, “Engineering Natural Product Biosynthesis in Oleaginous Yeast” (Ph.D. Expected 2022) Hilbert, Maxwell, “Fundamental Studies of Enzyme Immobilization” (Ph.D. Expected 2021)

Lee, Stephen, “Engineering Aromatic Metabolism in Oleaginous Yeast” (Ph.D. Expected 2023) McCarthy, Madeline, “Gene Interaction Screens with Multiplexing using Spectral Imaging and Combinatorics (MuSIC)” (Ph.D. Expected 2023; co-advised with Marc Birtwistle) Rives, Dyllan, “Cellular engineering of protein and enzyme production” (Ph.D. Expected 2021) Spagnuolo, Michael, “Metabolic Engineering of Yarrowia lipolytica for Materials Synthesis” (Ph.D. Expected 2020)

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Wang, Charles, “Developing a Human Artificial Chromosome for Large-Scale Gene Expression in Mammalian Cells” (MS Expected 2019; Co-Advised with Marc Birtwistle) Wang, Weigao, “Engineering Robust Enzyme Activity” (Ph.D. Expected 2020)

Wintenberg, Molly, “Engineering of Biological Radiation Sensors” (Ph.D. Expected 2021) Yaguchi, Allison, “Engineering Aromatic Metabolism in Oleaginous Yeast” (Ph.D. Expected 2019)

Current Postdoctoral Advising

Iqbal, Shoaib, “Nanoparticle delivery of genome editing components across the blood brain barrier” (October 1, 2018 – Current). Co-advised with Jessica Larsen and Angela Alexander-Bryant. Lu, Xiaoming, “Mammalian Artificial Chromosomes for delivering large sets of genes to CHO cells” (Jul 1, 2019 – Current). Co-advised with Marc Birtwistle. Somasundaram, Sivachandrian “Engineering Oleochemical Biosynthesis in Oleaginous Yeast” (November 15, 2019 – Current). Yang, Zhiliang, “Engineering Natural Product Biosynthesis in Oleaginous Yeast” (October 1, 2019 – Current).

Former Postdoctoral Advising Rodriguez, Gabriel, “Fatty alcohol production in Yarrowia lipolytica” (December 2014-

February 2017). Currently at Ginkgo Bioworks. Gao, Difeng, “Engineering omega-3 production in Yarrowia lipolytica by rerouting lipid metabolism” (February 2015-April 2018). Currently at BioMedican.

Technical Staff Supervision

Beitz, Adam – Lab Technician (May 2019 – August 2019) Anglin, Scott – Lab Manager (March 2017 – April 2019)

Smith, Spencer – Lab Technician (May 2017 – June 2018) Gambill, Lauren – Lab Technician (May 2017 – August 2017)

Wiseman, William – Lab Technician (May 2016 – August 2016) Cook, Taylor – Lab Technician (May 2015 – August 2015)

UNDERGRADUATE STUDENT ADVISING Departmental Honors Graduates

Taylor Cook. “Towards engineering selective biosensors using continuous evolution” (2015; ChBE)

Erika Arvay. “Improving bioprocesses using genetic and enzymatic dynamic control of metabolic pathways” (2017, ChBE)

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Matt Brabender. “Metabolic engineering for medium chain fatty alcohol production from animal fats and plant oils” (Expected 2020, ChBE)

UNDERGRADUATE RESEARCHERS

99 Total (‡ 4 URM; * 52 Woman; @ 2 High School; # 11 Grad School)

UNDERGRADS PROGRESSING TO GRADUATE SCHOOL 1. Taylor Cook – PhD in ChE at University of Wisconsin in the Brian Pfleger Group 2. James Foster – PhD in ChE at Clemson University in the Scott Husson Group 3. Erika Arvay – PhD in ChE at Northwestern University in the Keith Tyo Group 4. Lauren Gambill – PhD in Molecular Biosciences at Rice University in the James

Chappell Group 5. William Wiseman – MS in Biotechnology at Northwestern University in the Mike Jewett

Group # 6. Kyle Pazzo – MD at Medical University of South Carolina 7. Tanner Karp – MD at USC Medical School 8. Spencer Smith –PhD in Molecular Sciences in the Green Lab at Arizona State University 9. Michael Xie (Ohio) – PhD in Molecular Engineering at the University of Washington in

the Aimee Dudley Group. 10. Meredith Bailey – PhD in ChE at Dartmouth University. 11. Adam Beitz – PhD in ChE at MIT.

STUDENT HONORS & AWARDS 1. GAANN Fellowship (D. Rives, 2019) 2. Goldwater Scholarship Winner (E. Mihealsick, 2019) 3. ChBE Research Symposium, Honorable Mention in Poster Presentation (M. Wintenberg,

2019) 4. ChBE Research Symposium, 2nd Place in Student Choice Oral Presentation (M.

Spagnuolo, 2019) 5. DOE SCGSR Award (A. Yaguchi, 2019) 6. GAANN Fellowship (A. Yaguchi, 2019) 7. DTRA Life Sciences Technical Review, First Place in Poster Presentation (M. Hilbert,

2018) 8. ChBE Research Symposium, First Place in Poster Presentation, (A. Yaguchi, 2018) 9. ChBE Research Symposium, Honorable Mention in Poster Presentation (M. Wintenberg,

2018) 10. ChBE Research Symposium, Honorable Mention in Poster Presentation (M. Hilbert,

2018)

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11. NASA SC Space Grant Undergraduate Research Fellowship (A. Beitz, 2018) 12. NASA SC Space Grant Undergraduate Research Fellowship (M. Bailey, 2018) 13. NASA SC Space Grant Undergraduate Research Fellowship (M. Brabender, 2018) 14. SC EPSCoR Undergraduate Poster Competition - 2nd Place (M. Bailey, 2018) 15. Goldwater Scholarship Honorable Mention (E. Mihealsick, 2018) 16. ASM Microbe Outstanding Abstract Award (A. Yaguchi, 2018) 17. SBFC39 Poster Session Finalist (A. Yaguchi, 2017) 18. ChBE Graduate Researcher of the Year (M. Shabbir Hussain, 2017) 19. ChBE Teaching Assistant of the Year (A. Yaguchi, 2017) 20. NASA SC Space Grant Undergraduate Research Fellowship (M. Brabender, 2017) 21. NASA Center Internship (M. Brabender, 2017) 22. ChBE Research Symposium, First Place in Oral Presentation (M. Shabbir Hussain, 2017) 23. ChBE Research Symposium, First Place in Poster Competition (A. Yaguchi, 2017) 24. NSF Graduate Research Fellowship Program – Awardee (L. Gambill, 2017) 25. NSF Graduate Research Fellowship Program – Honorable Mention (E. Arvay,

2017) 26. CU College of Science Outstanding Undergraduate Research Award (L. Gambill,

2017) 27. SIMB Travel Award to SBFC Meeting (A. Yaguchi, 2017) 28. Goldwater Scholarship Winner (J. Zielinski, 2017) 29. Fulbright Fellowship Semifinalist (K. Scola, 2017) 30. Biomolecular Engineering REU – Best Poster (L. Gambill, 2016) 31. DOE Travel Award to SIMB Annual Meeting (M. Shabbir Hussain, 2016) 32. NASA SC Space Grant Undergraduate Research Fellowships (E. Arvay, 2016) 33. ACCAIC Creativity & Innovation Fellowship (S. Smith, 2016) 34. ACCAIC Creativity & Innovation Fellowship (E. Arvay, 2016) 35. Southeast Regional AIChE Poster Presentation Finalist (W. Wiseman, 2016) 36. ChBE Research Symposium, Honorable Mention in poster competition (A. Yaguchi,

2016) 37. Calhoun Honors College Award (E. Arvay, 2016, 2017) 38. ASM – South Carolina Fall Meeting, 2nd Place Graduate Student (M. Shabbir Hussain,

2015) 39. NASA SC Space Grant Undergraduate Research Fellowships (E. Arvay, 2015) 40. ACCAIC Creativity & Innovation Fellowship (L. Gambill, 2015) 41. ACCAIC Creativity & Innovation Fellowship (K. Pazzo, 2015) 42. Calhoun Honors College Award (S. Williams, 2014)

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43. Calhoun Honors College Award (T. Cook, 2014) 44. NASA SC Space Grant Undergraduate Research Fellowships (T. Cook, 2014) 45. NASA SC Space Grant Undergraduate Research Fellowships (J. Redzikowski, 2014) 46. ChBE Research Symposium, 2nd Place in Poster Competition (M. Shabbir Hussain, 2014)

TEACHING Courses Taught

ChE 4070, Unit Operations Lab II, F12 ChE 3300, Mass Transfer & Separation, S13, S14, S15 ChE 4500, Chemical Reaction Engineering, F13, F14, F15 BMOLE 4250/6250, Biomolecular Engineering, S15, S16, S17, S18, S19 ChE 8050, Graduate Reaction Kinetics, F16, F17, F18 ChE 8450, Special Topics: Modern Biomolecular Engineering, F17 ChE 3950, Honors Research, F13, S14, F14, S15, S16, F17, S18, S19 ChE 4950, Honors Research, F14, F16 ChE 4970, Honors Thesis, S15, S17 ChE X990, Creative Inquiry –Sustainable Chemicals from Yeast, F15, S16, F16, S17, F17, S18, F18, S19 ChE X990, Creative Inquiry –Sustainable Chemicals from Bacteria, S13, F13, S14, F14, S15, F15, S16, F16, S17, F17, S18, F18, S19 ChE X990, Creative Inquiry – Protein Therapeutics, F13, S14, F14, S15, F15, S16, F16, S17 ChE X990, Creative Inquiry – Getting to Mars with Microbes, F17, S18, F18, S19 ChE X990, Creative Inquiry - Human Artificial Chromosomes as a Platform for Multiplexed Expression of CRISPR-Based Genetic Recording System, F18, S19 ChE X990, Creative Inquiry – Engineering Biopharmaceutical Production from Mammalian Cell Culture, S19 CES 1900, Mentor Guided Inquiry – Su14 ChE 8910, Masters Research, F14, S15, S18, F18, S19 ChE 9910, Doctoral Research, S13, Su13, F13, S14, Su14, F14, S15, Su15, F15, S16, Su16, F16, S17, Su17, F17, S18, Su18, F18, S19, Su19, F19

Courses Developed

ChE 8450, Special Topics: Modern Biomolecular Engineering BMOLE 4250/6250, Biomolecular Engineering ChE 1990, 2990, 3990, 4990, Yeast Engineering Creative Inquiry ChE 1990, 2990, 3990, 4990, Bacteria Engineering Creative Inquiry ChE 1990, 2990, 3990, 4990, Protein Therapeutics Creative Inquiry

-15-

ChE 1990, 2990, 3990, 4990, Getting to Mars with Microbes Creative Inquiry ChE 1990, 2990, 3990, 4990, Human Artificial Chromosome Creative Inquiry ChE 1990, 2990, 3990, 4990, Biopharmaceutical Production Creative Inquiry

October 22, 2019