clip. 1 bq 20 wl 10 quality: 0 - 9 sequence: cmvfor cmvfor ...€¦ · page: 1 / 3 22.05.2019...
TRANSCRIPT
Page: 1 / 322.05.2019
Sequence: CMVfor_CMVfor Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8771288853.4
Samples:Bases:Average spacing:
12964102213.0
Average quality >= 10: 45, 20: 69, 30: 821
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C G AA T G G C T G10
A T T G C T C TA G20
C G C CA C C A T G30
A A A C A G G C A G40
C A G C C A C A G G50
T A A G A T T G C T60
G T T G C A C A G T70
G A T T C G G G A T80
G T T T
A A T A C C90
A C T A C A A G A A100
A T T G A G A T G G110
T G C A C T A C C A120
C T A C A A T G G A130
T G T C A T C G G A140
A G A G A G T A G T150
T T T T C A C T T C160
A C A G G T C
G A T170
A A G C A T G C G T180
A G G A G A A A G A190
G T C T T A A G T A200
A T C T A G A T A A210
C G A G C C C A G C220
A A C A C C A A G G230
A T T A G G A C A A240
G G C T G G T G G T250
G
A G T T T C G T C260
A C C A G T A C A G270
G A A A C A G C T A280
T G A C C T G T A A290
A A C G A C G G C C300
A G T A G G G T T T310
T C C C A G T C A C320
G A C G T T G G T C330
G T C A G
A C T G T340
C G A T G A A G C C350
C G T T C G A C C C360
C G C C T C G A T C370
C G G C T A A C T A380
G A G A A C C C A C390
T G G G C A A C T A400
G A A G G C A C A G410
T C A G A G C T C
G420
T T T A G T G A A C430
C G G T G G T T T G440
T C C A A A C T C A450
T C T A G A A G G C460
A C A G T C G A G G470
G A T C A C T C T C480
G G C A T G G A C C490
A T T T T A T G T T500
T C
Page: 2 / 322.05.2019
Sequence: CMVfor_CMVfor Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8771288853.4
Samples:Bases:Average spacing:
12964102213.0
Average quality >= 10: 45, 20: 69, 30: 821
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A G G T T C A G510
G G G T C G T A A C520
A A C T C C G C C C530
G T C C A G C T C G540
A C C A G G A T G A550
G G T T A A G T A A560
A G C G T C T G A C570
C G T A A G T A G C580
A T C A C C
T T C T590
G G T C T T G T T A600
G A A T T T G T T A610
C C T T T T C G G T620
T A G A G C G G A T630
G T G T C G C G T T640
A A C G C T A G C A650
T G G A T C T C A C660
A T C A G A G A
T T670
T T G A G A C A C G680
G G C G G A T A T G690
T A T A T G G T G G700
T A A T G C T G A C710
C A A A C C T C T G720
G C G A A G A T G C730
G T C C G G C G T A740
G A T C C G G A T T750
A T T C A T A C C G760
T C C C C C T C T A770
C A A A T G T G G T780
A T G G C T G A T T790
A T G A T C A G A T800
A G C A T G G C C T810
T T G C A G G G T A820
T C T T A T G G T
A830
C T G T A A C T G C840
T T T A T G T T T T850
T G G C G T C T T C860
C C T A G C A A A A870
T A C G C T G T C C880
C A A G A A C A T C890
G A T T T T C C A T900
G G C A
G G C C T G910
A C A C C A T A T C920
C A T C G C A G C T930
G A G A T A T T G T940
A G A G A T C A A A950
T G A T A C A A T C960
T C G C G G G
Page: 3 / 322.05.2019
Sequence: CMVfor_CMVfor Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8771288853.4
Samples:Bases:Average spacing:
12964102213.0
Average quality >= 10: 45, 20: 69, 30: 821
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T T C970
A A G T T C C T G C980
A A T A A C T G C A990
A T C T A T C A A C1000
A A G G A G G A T T1010
C T T A T T T A T A1020
A
Page: 1 / 322.05.2019
Sequence: DuetDOWN1_DuetDOWN1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101017102651.14
Samples:Bases:Average spacing:
16302124814.0
Average quality >= 10: 112, 20: 103, 30: 921
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C GG C C T GG G A10
A GG G C G G A A T20
G T A T A T G G T G30
G T AA T G C T G A40
C C A A A C C T C T50
G G C G A A G A T G60
C G T C C G G C G T70
A G A T C C G G A T80
T A
T T C A T A C C90
G T C C C C C T C T100
A C A A A T G T G G110
T A T G G C T G A T120
T A T G A T C A G A130
T A G C A T G G C C140
T T T G C A G G G T150
A T C T T A T G G T160
A C T G T A A
C T G170
C T T T A T G T T T180
T T G G C G T C T T190
C C C T A G C A A A200
A T A G G C T G T C210
C C A A G A A C A T220
C G A T T T T C C A230
T G G C A G G C C T240
G A A C A C C A T A250
T C C A T C C G C A260
G C T G A G A A T A270
T T G T A G G A G A280
T C A A A A T G A T290
A A C C A T C T C G300
C G G G T C C A A G310
T T T C C C T G C A320
T T A C T G G A T C330
T A T C A
A C A G G340
A G G G T C T A T A350
T A A G C A G A G C360
T G A A T C T G T G370
T G G G C A C T C G380
C T T C T G C G T T390
C T G A T T T A A T400
C T G C A T T T A G410
G T G A C A C T A
T420
A G A A T T A A C C430
C T C A C T A A A G440
G G T A A T A C G A450
C T C A C T A T A G460
G G A G A G C G G C470
T G T C T G G A T C480
T A C G T A A T A C490
G C T A G T T A C T500
T T
Page: 2 / 322.05.2019
Sequence: DuetDOWN1_DuetDOWN1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101017102651.14
Samples:Bases:Average spacing:
16302124814.0
Average quality >= 10: 112, 20: 103, 30: 921
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A T C C A G C C510
C T C A C T A G G T520
C C T T T G T A G C530
T T T C T G G C A A540
G G T A G A C A A G550
C C C A T T G C T G560
A T T A G A G G A G570
T C A A C C G T A G580
A A T C G
A G A C C590
G A G G A G A G G G600
C A T T G G G T C A610
A C A G T A T A G A620
A C C G T G G A T G630
A T G T T G C C A C640
C T T T A T G T A T650
G T A C G G T T A C660
C T T G T T A C
G A670
C T T A C G G G C G680
G T G T G T G T A C690
A G A G T G A C T A700
T C A T A T G C T T710
A C C G T G A G A G720
G T C C A C T G A C730
G G G T G C C C C G740
C T C A A C T T C A750
G
G A T A C G G T C760
C T G G G T G C T G770
T T C G T G G T T A780
A T T A A T C T A G790
A G C T A G C G A A800
T T C C T G T G T G810
A A A T T G T A T C820
G C G G G T G G C A830
G C T
C T A G A G C840
T A G C G A A T T C850
T T T G G T G A A A860
T T G T T A T C C G870
C T C A C A A T T C880
C A C A C A A C A T890
A C G A G C C G G A900
A G C A T A A A G T910
G T A A A
G C C T G920
G G G T G C C T A A930
T G A G T G A G C T940
A A C T C A C A T T950
A A T T G C G T T G960
C G C T C A C T G C970
C C G C T T T C C A980
G T C G G G A A A C990
C T G T
Page: 3 / 322.05.2019
Sequence: DuetDOWN1_DuetDOWN1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101017102651.14
Samples:Bases:Average spacing:
16302124814.0
Average quality >= 10: 112, 20: 103, 30: 921
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C G T G C C1000
A G C T G C A T T A1010
A T G A A T C G G C1020
C A A C G C G C G G1030
G G A G A G G C G G1040
T T T G C G T A T T1050
G G C G C T C T T C1060
G C T T C C T C
G C1070
T C A C T G A C T C1080
G C T G C G C T C G1090
G T C G T C G G C T1100
G C G G C G A G C G1110
T A T C A G C T C A1120
C T C A A G G C G G1130
T T A T A C
G C T A1140
T C A CA G A T C A1150
G G G A T A C G C A1160
A G G A A G A A C A1170
T G T G A G C A A A1180
G C C C A G C A G
C1190
C A G A A C C G T T1200
A A A A G G C C G C1210
G A T G C C T G GC1220
G T T T T C C A A T1230
A A G G T T
C C G C1240
C C C C C TG
Page: 1 / 322.05.2019
Sequence: DuetUP2_DuetUP2 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
102111103151.6
Samples:Bases:Average spacing:
16299123714.0
Average quality >= 10: 100, 20: 90, 30: 929
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G G G C T C A G C G10
TT A C G C G A CA20
C A T C C G C T C T30
A A C C G A A A A G40
G T A A C A A A T T50
C T A A C A A G A C60
C A G A A G G T G A70
T G C T A C T T A C80
G G
T C A G A C G C90
T T T A C T T A A C100
C T C A T C C T G G110
T C G A G C T G G A120
C G G G C G G A G T130
T G T T A C G A C C140
C C T G A A C C T G150
A A A C A T A A A A160
T G G T C C
A T G G170
G A A T G T G T G T180
C A G T T A G G G T190
C C G A G A G T G A200
T C C C T C G A C T210
G T G C C T T C T A220
G A T G A G T T T G230
G A C A A A C C A C240
C G G T T C A C T A250
A A C G A G C T C T260
G A C T G T G A C T270
T C T A G T T G C C280
C A G T G G G T T C290
T C T A G T T A G C300
C G G A T C G A G G310
C G G G G T C G A A320
C G G G C T T C A T330
C G A C
A G T C T G340
A C G A C C A A C G350
T C G T G A C T G G360
G A A A A C C C T A370
C T G G C C G T C G380
T T T T A C A G G T390
C A T A G C T G T T400
T C C T G T A C T G410
G T G A C G A A
A C420
T C A C T T G G T G430
T T G C T G G G C T440
C G T T A T C T A G450
A T T A C T T A A G460
A C T C T T T C T C470
C T A C G C A T G C480
T T A T C G A C C T490
G T G A A G T G A A500
A A
Page: 2 / 322.05.2019
Sequence: DuetUP2_DuetUP2 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
102111103151.6
Samples:Bases:Average spacing:
16299123714.0
Average quality >= 10: 100, 20: 90, 30: 929
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A C T A C T C T510
C T T C C G A T G A520
C A T C C A T T G T530
A G T G G T A G T G540
C A C C A T C T C A550
A T T T C T T G T A560
G T G G T A T T A A570
A C A T C C C G A A580
T C A C T
G T G C A590
A C C T G C C T G T600
T T C A T G G T G G610
C G C T A G A G C A620
A A A C A G C G T G630
G A T G G C G C A C640
G C C T A C C G C C650
C A T T T G C G G C660
A G C A A T G C
T G670
G C A A T A G T A G680
C T T G T C A A T T690
G G A A C C A G T C700
G G A T G T C A G A710
A T G C C A T T T G720
G A A T C A T G G G730
A A A T A G G C C C740
T C C G T T A A T T750
A A C T C G A G G T760
C G A C C A T A T G770
G T G C A C T C T C780
A G T A C A A T C T790
G C T C T G A T G C800
C G C T A C G G G C810
G C G C C C T C G A820
G G T C G A C C A A830
T T
G C C A A A C T840
C T C A G T A C A A850
T C T G C T C T G A860
T G C C G C A T A G870
T T A A G C C A G C880
C C C G A C A C C C890
G C C A A C A C C C900
G C T G A C G C G C910
C C T
G A C G G G C920
T T G T C T G C T C930
C C G G C A T C C G940
C T T A C A G A C A950
A G C T G T G A C C960
G T G T C C G G G A970
G C T G C A T G T G980
T C A G A G G T T T990
T C
Page: 3 / 322.05.2019
Sequence: DuetUP2_DuetUP2 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
102111103151.6
Samples:Bases:Average spacing:
16299123714.0
Average quality >= 10: 100, 20: 90, 30: 929
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A C C G T C A T1000
C A C C G A A A C G1010
C G C G A T A C G A1020
A A G G G C C T C G1030
T G A T A C G C C T1040
A T T T T A T A G G1050
T A A T G T C A T G1060
A T A A T A A
T G G1070
T T T C T T A G A C1080
G T C A G T G C A C1090
T T T T C G G G A A1100
A T G T G C G C G G1110
A C C C T A T T G T1120
T A T T T T C T A
A1130
T T A C A T C A A T1140
A T G T A T C C G C1150
T C A T G A A C C A1160
T T A C C C T T G A1170
T A A T G C T C C A1180
A T A A A T G
A A A1190
C G G A GAGC TA1200
G G A T A T C A C A1210
T T C GT T C G C C1220
C T T A T T T
C C T1230
T T T T T G
Page: 1 / 322.05.2019
Sequence: M13 rev (-29)_M13rev-29 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8761589053.8
Samples:Bases:Average spacing:
12966101313.0
Average quality >= 10: 46, 20: 50, 30: 836
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A A C G G A C G TA10
C G G T T T C CA G20
T C A C G A C G T T30
G G T C G T C A G A40
C T G T C G A T G A50
A G C C C G T T C G60
A C C C C G C C T C70
G A T C C G G C T A80
A
C T A G A G A A C90
C C A C T G G G C A100
A C T A G A A G G C110
A C A G T C A G A G120
C T C G T T T A G T130
G A A C C G G T G G140
T T T G T C C A A A150
C T C A T C T A G A160
A G G C A
C A G T C170
G A G G G A T C A C180
T C T C G G C A T G190
G A C C A T T T T A200
T G T T T C A G G T210
T C A G G G G T C G220
T A A C A A C T C C230
G C C C G T C C A G240
C T C G A C C A G
G250
A T G A G G T T A A260
G T A A A G C G T C270
T G A C C G T A A G280
T A G C A T C A C C290
T T C T G G T C T T300
G T T A G A A T T T310
G T T A C C T T T T320
C G G T T A G A G C330
G G A
T G T G T C G340
C G T T A A C G C T350
A G C A T G G A T C360
T C A C A T C A G A370
G A T T T T G A G A380
C A C G G G C G G A390
T A T G T A T A T G400
G T G G T A A T G C410
T G A C C A A
A C C420
T C T G G C G A A G430
A T G C G T C C G G440
C G T A G A T C C G450
G A T T A T T C A T460
A C C G T C C C C C470
T C T A C A A A T G480
T G G T A T G G C T490
G A T T A T G A T C500
A
Page: 2 / 322.05.2019
Sequence: M13 rev (-29)_M13rev-29 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8761589053.8
Samples:Bases:Average spacing:
12966101313.0
Average quality >= 10: 46, 20: 50, 30: 836
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G A T A G C A T G510
G C C T T T G C A G520
G G T A T C T T A T530
G G T A C T G T A A540
C T G C T T T A T G550
T T T T T G G C G T560
C T T C C C T A G C570
A A A A T A G G C T580
G T C
C C A A G A A590
C A T C G A T T T T600
C C A T G G C A G G610
C C T G A A C A C C620
A T A T C C A T C C630
G C A G C T G A G A640
A T A T T G T A G G650
A G A T C A A A A T660
G A T A A C
C A T C670
T C G C G G G T C C680
A A G T T T C C C T690
G C A T T A C T G G700
A T C T A T C A A C710
A G G A G G G T C T720
A T A T A A G C A G730
A G C T G A A T C T740
G T G T G G G
C A C750
T C G C T T C T G C760
G T T C T G A T T T770
A A T C T G C A T T780
T A G G T G A C A C790
T A T A G A A T T A800
A C C C T C A C T A810
A A G G G T A A T A820
C G A C T C
A C T A830
T A G G G A G A G C840
G G C T G T C T G G850
A T C T A C G T A A860
T A C G C T A G T T870
A T T G C T C A G C880
G G T C C T T T A T890
A C A T T A G T C C900
T T T G T A G C T T910
T C T G G C A A G G920
T A G A C A A G C C930
C A T T G C T G A T940
T A G A G G A G T C950
A C C G T A G A T C960
G A G A C C
Page: 3 / 322.05.2019
Sequence: M13 rev (-29)_M13rev-29 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8761589053.8
Samples:Bases:Average spacing:
12966101313.0
Average quality >= 10: 46, 20: 50, 30: 836
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G A A G970
A G A G G C C A T G980
G G T C A C A G T A990
T A G A A C C G T G1000
G A T G A A T G T T1010
T G
Page: 1 / 322.05.2019
Sequence: M13 rev (-49)_M13rev-49 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9121692752.48
Samples:Bases:Average spacing:
12964103613.0
Average quality >= 10: 58, 20: 68, 30: 833
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A G C C A T G C T C10
A GG A T T A T T A20
A C CA C G A A C A30
G C A C C C A G G A40
C C G T A T C C T G50
A A G T T G A G C G60
G G G C A C C C G T70
C A G T G G A C C T80
C T C
A A C T C T C90
C C G C T G A G C C100
A T G A T C A A A C110
T C T G T A C A C A120
C A C C G C C C G T130
A A G T C G T A A C140
A A G G T A A C C G150
T A C A T A C A T A160
A A G G T G G
C A A170
C A T C A T C C A C180
G G T T C T A T A C190
T G T T G A C C C A200
A T G C C C T C T C210
C T C G G T C T C G220
A T T C T A C G G T230
T G A C T C C T C T240
A A T C A G C A A T250
G
G G C T T G T C T260
A C C T T G C C A G270
A A A G C T A C A A280
A G G A C C T A A T290
G T A T A A G G A C300
C G C T G A G C A A310
T A A C T A G C G T320
A T T A C G T A G A330
T C C A G A
C A G C340
C G C T C T C C C T350
A T A G T G A G T C360
G T A T T A C C C T370
T T A G T G A G G G380
T T A A T T C T A T390
A G T G T C A C C T400
A A A T G C A G A T410
T A A A T C A G A A420
C G C A G A A G C G430
A G T G C C C A C A440
C A G A T T C A G C450
T C T G C T T A T A460
T A G A C C C T C C470
T G T T G A T A G A480
T C C A G T A A T G490
C A G G G A A A C T500
T G G A
Page: 2 / 322.05.2019
Sequence: M13 rev (-49)_M13rev-49 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9121692752.48
Samples:Bases:Average spacing:
12964103613.0
Average quality >= 10: 58, 20: 68, 30: 833
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C C G C G510
A G A T G G T T A T520
C A T T T T G A T C530
T C C T A C A A T A540
T T C T C A G C T G550
C G G A T G G A T A560
T G G T G T T C A G570
G C C T G C C A T G580
G A A A A T C
G A T590
G T T C T T G G G A600
C A G C C T A T T T610
T G C T A G G G A A620
G A C G C C A A A A630
A C A T A A A G C A640
G T T A C A G T A C650
C A T A A G A T A C660
C C T G C A A A G G670
C C A T G C T A T C680
T G A T C A T A A T690
C A G C C A T A C C700
A C A T T T G T A G710
A G G G G G A C G G720
T A T G A A T A A T730
C C G G A T C T A C740
G C C G G A C G C A750
T C T
T C G C C A G760
A G G T T T G G T C770
A G C A T T A C C A780
C C A T A T A C A T790
A T C C G C C C G T800
G T C T C A A A A T810
C T C T G A T G T G820
A G A T C C A T G C830
T A G C
G T T A A C840
G C G A C A C A T C850
C G C T C T A A C C860
G A A A A G G T A A870
C A A A T T C T A A880
C A A G A C C A G A890
A A G T G A T G C T900
A C T T A C G G T C910
A G
A C G C T T T A920
C T T A A C C T C A930
T C C T G C T C G A940
G C T G G A C G G C950
G A G T G T A C G A960
C C C C C T G A C T970
G A A A C A T A A
Page: 3 / 322.05.2019
Sequence: M13 rev (-49)_M13rev-49 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9121692752.48
Samples:Bases:Average spacing:
12964103613.0
Average quality >= 10: 58, 20: 68, 30: 833
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A980
T G T C A T G C G A990
G A G T G A T C C C1000
T C G A C T G T G C1010
C C T T T T C T A G1020
A A T G G A G T T T1030
G G A C C
Page: 1 / 322.05.2019
Sequence: M13 uni (-21)_M13uni-21 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8741088352.7
Samples:Bases:Average spacing:
12962101813.0
Average quality >= 10: 58, 20: 57, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A GG AA T A A C G10
A CG T T G G T C G20
T CA G A C T G T C30
G A T G A G C C C G40
T T C G A C C C C G50
C C T C G A T C C G60
G C T A A C T A G A70
G A A C C C A C T G80
G G C
A A C T A G A90
A G G C A C A G T C100
A G A G C T C G T T110
T A G T G A A C C G120
G T G G T T T G T C130
C A A A C T C A T C140
T A G A A G G C A C150
A G T C G A G G G A160
T C A C T C T
C G G170
C A T G G A C C A T180
T T T A T G T T T C190
A G G T T C A G G G200
G T C G T A A C A A210
C T C C G C C C G T220
C C A G C T C G A C230
C A G G A T G A G G240
T T A A G T A A A G250
C
G T C T G A C C G260
T A A G T A G C A T270
C A C C T T C T G G280
T C T T G T T A G A290
A T T T G T T A C C300
T T T T C G G T T A310
G A G C G G A T G T320
G T C G C G T T A A330
C G C T A G
C A T G340
G A T C T C A C A T350
C A G A G A T T T T360
G A G A C A C G G G370
C G G A T A T G T A380
T A T G G T G G T A390
A T G C T G A C C A400
A A C C T C T G G C410
G A A G A T G C G
T420
C C G G C G T A G A430
T C C G G A T T A T440
T C A T A C C G T C450
C C C C T C T A C A460
A A T G T G G T A T470
G G C T G A T T A T480
G A T C A G A T A G490
C A T G G C C T T T500
G C A
Page: 2 / 322.05.2019
Sequence: M13 uni (-21)_M13uni-21 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8741088352.7
Samples:Bases:Average spacing:
12962101813.0
Average quality >= 10: 58, 20: 57, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G G G T A T C510
T T A T G G T A C T520
G T A A C T G C T T530
T A T G T T T T T G540
G C G T C T T C C C550
T A G C A A A A T A560
G G C T G T C C C A570
A G A A C A T C G A580
T T T T C C
A T G G590
C A G G C C T G A A600
C A C C A T A T C C610
A T C C G C A G C T620
G A G A A T A T T G630
T A G G A G A T C A640
A A A T G A T A A C650
C A T C T C G C G G660
G T C C A A G T
T T670
C C C T G C A T T A680
C T G G A T C T A T690
C A A C A G G A G G700
G T C T A T A T A A710
G C A G A G C T G A720
A T C T G T G T G G730
G C A C T C G C T T740
C T G C G T T C T
G750
A T T T A A T C T G760
C A T T T A G G T G770
A C A C T A T A G A780
A T T A A C C C T C790
A C T A A A G G G T800
A A T A C G A C T C810
A C T A T A G G G A820
G A G C G G C T
G T830
C T G G A T C T A C840
G T A A T A C G C T850
A G T T A T T G C T860
C A G C G G T C C T870
T A T A C A T T A G880
T C C T T T G T A G890
C T T T C T G G C A900
A
G G T A G A C A G910
C C C A T T G C T G920
A T T A G A G A G T930
C A A C C G T A G A940
T C G A G A C G A G950
G A G A G G G C A T960
T G G G
Page: 3 / 322.05.2019
Sequence: M13 uni (-21)_M13uni-21 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8741088352.7
Samples:Bases:Average spacing:
12962101813.0
Average quality >= 10: 58, 20: 57, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T T C A A C970
A G T A T A G A T C980
G T C A T G A T G G990
T T G G C A C C A T1000
T A TA T G T T A T1010
G T T A A C G
Page: 1 / 322.05.2019
Sequence: M13 uni (-43)_M13uni-43 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8991190953.0
Samples:Bases:Average spacing:
12964102313.0
Average quality >= 10: 61, 20: 48, 30: 838
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G G A C T T G A C T10
G T C G A T G A G C20
C C G T T C G A C C30
C C G C C T C G A T40
C C G G C T A A C T50
A G A G A A C C C A60
C T G G G C A A C T70
A G A A G G C A C A80
G T C A
G A G C T C90
G T T T A G T G A A100
C C G G T G G T T T110
G T C C A A A C T C120
A T C T A G A A G G130
C A C A G T C G A G140
G G A T C A C T C T150
C G G C A T G G A C160
C A T T T T A
T G T170
T T C A G G T T C A180
G G G G T C G T A A190
C A A C T C C G C C200
C G T C C A G C T C210
G A C C A G G A T G220
A G G T T A A G T A230
A A G C G T C T G A240
C C G T A A G T A G250
C
A T C A C C T T C260
T G G T C T T G T T270
A G A A T T T G T T280
A C C T T T T C G G290
T T A G A G C G G A300
T G T G T C G C G T310
T A A C G C T A G C320
A T G G A T C T C A330
C A T C A
G A G A T340
T T T G A G A C A C350
G G G C G G A T A T360
G T A T A T G G T G370
G T A A T G C T G A380
C C A A A C C T C T390
G G C G A A G A T G400
C G T C C G G C G T410
A G A T C C G G
A T420
T A T T C A T A C C430
G T C C C C C T C T440
A C A A A T G T G G450
T A T G G C T G A T460
T A T G A T C A G A470
T A G C A T G G C C480
T T T G C A G G G T490
A T C T T A T G G T500
A C
Page: 2 / 322.05.2019
Sequence: M13 uni (-43)_M13uni-43 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8991190953.0
Samples:Bases:Average spacing:
12964102313.0
Average quality >= 10: 61, 20: 48, 30: 838
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T G T A A C T G510
C T T T A T G T T T520
T T G G C G T C T T530
C C C T A G C A A A540
A T A G G C T G T C550
C C A A G A A C A T560
C G A T T T T C C A570
T G G C A G G C C T580
G A A C A
C C A T A590
T C C A T C C G C A600
G C T G A G A A T A610
T T G T A G G A G A620
T C A A A A T G A T630
A A C C A T C T C G640
C G G G T C C A A G650
T T T C C C T G C A660
T T A C T G G A
T C670
T A T C A A C A G G680
A G G G T C T A T A690
T A A G C A G A G C700
T G A A T C T G T G710
T G G G C A C T C G720
C T T C T G C G T T730
C T G A T T T A A T740
C T G C A T T T A
G750
G T G A C A C T A T760
A G A A T T A A C C770
C T C A C T A A A G780
G G T A A T A C G A790
C T C A C T A T A G800
G G A G A G C G G C810
T G T C T G G A T C820
T A C G T A A T
A C830
G C T A G T T A T T840
G C T C A G C G G T850
C C T T A T A C A T860
T A G G T C C T T T870
G T A G C T T T C T880
G G C A A G G T A G890
A C A A G C C C A T900
T G
C T G A T T A G910
A G G A G T C A A C920
C G T A G A A T C G930
A G A C C G A G G A940
G A G G G C A T T G950
G T C A C A G T A T960
A G A A C C G T
Page: 3 / 322.05.2019
Sequence: M13 uni (-43)_M13uni-43 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8991190953.0
Samples:Bases:Average spacing:
12964102313.0
Average quality >= 10: 61, 20: 48, 30: 838
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T C970
A A T G A T G G T G980
G C A C G T T T A T990
G T T A T G T A C G1000
G C T T A C C C T T1010
G G T T A C G A A C1020
C T
Page: 1 / 322.05.2019
Sequence: pBABE3_pBABE3 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9421695753.44
Samples:Bases:Average spacing:
16301123814.0
Average quality >= 10: 87, 20: 88, 30: 917
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G GG A C A T T A10
G T T C T G T T A G20
G G G T C G T A C A30
A C T C C G C C C G40
T C C A G C T C G A50
C C A G G A T G A G60
G T T A A G T A A A70
G C G T C T G A C
C80
G T A A G T A G C A90
T C A C C T T C T G100
G T C T T G T T A G110
A A T T T G T T A C120
C T T T T C G G T T130
A G A G C G G A T G140
T G T C G C G T T A150
A C G C T A G C A T160
G G A
T C T C A C A170
G A T T A T G C G G180
C C G T G T A C A A190
T C A G A G A T T T200
T G A G A C A C G G210
G C G G A T A T G T220
A T A T G G T G G T230
A A T G C T G A C C240
A A A C C T
C T G G250
C G A A G A T G C G260
T C C G G C G T A G270
A T C C G G A T T A280
T T C A T A C C G T290
C C C C C T C T A C300
A A A T G T G G T A310
T G G C T G A T T A320
T G A T C A G A T A330
G
C A T G G C C T T340
T G C A G G G T A T350
C T T A T G G T A C360
T G T A A C T G C T370
T T A T G T T T T T380
G G C G T C T T C C390
C T A G C A A A A T400
A G G C T G T C C C410
A A G A A
C A T C G420
A T T T T C C A T G430
G C A G G C C T G A440
A C A C C A T A T C450
C A T C C G C A G C460
T G A G A A T A T T470
G T A G G A G A T C480
A A A A T G A T A A490
C C A T C T C G
Page: 2 / 322.05.2019
Sequence: pBABE3_pBABE3 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9421695753.44
Samples:Bases:Average spacing:
16301123814.0
Average quality >= 10: 87, 20: 88, 30: 917
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C G500
G G T C C A A G T T510
T C C C T G C A T T520
A C T G G A T C T A530
T C A A C A G G A G540
G G T C T A T A T A550
A G C A G A G C T G560
A A T C T G T G T G570
G G C A C T C G C T580
T
C T G C G T T C T590
G A T T T A A T C T600
G C A T T T A G G T610
G A C A C T A T A G620
A A T T A A C C C T630
C A C T A A A G G G640
T A A T A C G A C T650
C A C T A T A G G G660
A G A G
C G G C T G670
T C T G G A T C T A680
C G T A A T A C G C690
T A G T T A C T T T700
A T C C A G C C C T710
C A C T A G G T C C720
T T T G T A G C T T730
T C T G G C A A G G740
T A G A C A A
G C C750
C A T T G C T G A T760
T A G A G G A G T C770
A A C C G T A G A A780
T C G A G A C C G A790
G G A G A G G G C A800
T T G G G T C A A C810
A G T A T A G A A C820
C G T G G A T G A
T830
G T T G C C A C C T840
T T A T G T A T G T850
A C G G T T A C C T860
T G T T A C G A C T870
T A C G G G C G G T880
G T G T G T A C A G890
A G T G A C T A T C900
A T A T G C T T A C910
C G T G A G A G G T920
C C A C T G A C G G930
G T G C C C C G C T940
C A A C T T C A G G950
A T A C G G T C C T960
G G G T G C T G T T970
C G T G G T T T A A980
T T A A T C T A G A990
Page: 3 / 322.05.2019
Sequence: pBABE3_pBABE3 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9421695753.44
Samples:Bases:Average spacing:
16301123814.0
Average quality >= 10: 87, 20: 88, 30: 917
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G C T A G C G A A T1000
T C C T G T G T G A1010
A T T G T A T C G C1020
G G G G T G G C A G1030
C T C T A G A G C T1040
A G C G A A T T C T1050
T T G G T G A A A T1060
T G T T A T
C C G C1070
T C A C A A T T C C1080
A C CA C A C A T A1090
C G A G C C G G A G1100
C A T A A A G T G T1110
A A G C C T G G G G1120
T G C T A T G A G T1130
G A G
C T A C T C A1140
C A T A T G C G T G1150
C G C T C A C T G C1160
C G C T T T C A G T1170
C G G C A A C T G T1180
C TG C A
G C T G C1190
A T A T G G A A T C1200
G C C A C G C C C G1210
G G A A A G C C G A1220
T T G G C G T
A A T1230
T G G G G G G
Page: 1 / 322.05.2019
Sequence: pBABE5_pBABE5 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
99014100351.36
Samples:Bases:Average spacing:
16301125214.0
Average quality >= 10: 111, 20: 98, 30: 912
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C G T A C T T C10
T GG C A G T A G A20
C A G C C C A T T G30
C T G A T T A G A G40
G A G T C A A C C G50
T A G A A T C G A G60
A C C G A G G A G A70
G G G C A T T G G G80
T
C A A C A G T A T90
A G A A C C G T G G100
A T G A T G T T G C110
C A C C T T T A T G120
T A T G T A C G G T130
T A C C T T G T T A140
C G A C T T A C G G150
G C G G T G T G T G160
T A C A G
A G T G A170
C T A T C A T A T G180
C T T A C C G T G A190
G A G G T C C A C T200
G A C G G G T G C C210
C C G C T C A A C T220
T C A G G A T A C G230
G T C C T G G G T G240
C T G T T C G T G
G250
T T A A T T A A T C260
T A G A G C T A G C270
G A A T T C C T G T280
G T G A A A T T G T290
A T C G C G G G T G300
G C A G C T C T A G310
A G C T A G C G A A320
T T C T T T G G T G330
A A A
T T G T T A T340
C C G C T C A C A A350
T T C C A C A C A A360
C A T A C G A G C C370
G G A A G C A T A A380
A G T G T A A A G C390
C T G G G G T G C C400
T A A T G A G T G A410
G C T A A C T
C A C420
A T T A A T T G C G430
T T G C G C T C A C440
T G C C C G C T T T450
C C A G T C G G G A460
A A C C T G T C G T470
G C C A G C T G C A480
T T A A T G A A T C490
G G C C A A C G C G500
Page: 2 / 322.05.2019
Sequence: pBABE5_pBABE5 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
99014100351.36
Samples:Bases:Average spacing:
16301125214.0
Average quality >= 10: 111, 20: 98, 30: 912
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C G G G G A G A G G510
C G G T T T G C G T520
A T T G G G C G C T530
C T T C C G C T T C540
C T C G C T C A C T550
G A C T C G C T G C560
G C T C G G T C G T570
T C G G C T G C G G580
C G A
G C G G T A T590
C A G C T C A C T C600
A A A G G C G G T A610
A T A C G G T T A T620
C C A C A G A A T C630
A G G G G A T A A C640
G C A G G A A A G A650
A C A T G T G A G C660
A A A A G G
C C A G670
C A A A A G G C C A680
G G A A C C G T A A690
A A A G G C C G C G700
T T G C T G G C G T710
T T T T C C A T A G720
G C T C C G C C C C730
C C T G A C G A G C740
A T C A C A A A A
A750
T C G A C G C T C A760
A G T C A G A G G T770
G G C G A A A C C C780
G A C A G G A C T A790
T A A A G A T A C C800
A G G C G T T T C C810
C C C T G G A A G C820
T C C C T C G T G C830
G C
T C T C C T G T840
T C C G A C C C T G850
C C G C T T A C C G860
G A T A C C T G T C870
C G C C T T T C T C880
C C T T C G G G A A890
G C G T G G C G C T900
T T C T C A T A G C910
T C A
C G C T G T A920
G T A T C T C A G T930
T C G G T G T A G G940
T C G T T C G C T C950
C A A G C T G G G C960
T G T G T G C A C G970
A A C C C C C C G T980
T C A G C C C G A C990
C
Page: 3 / 322.05.2019
Sequence: pBABE5_pBABE5 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
99014100351.36
Samples:Bases:Average spacing:
16301125214.0
Average quality >= 10: 111, 20: 98, 30: 912
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G C T G C G C C T1000
T A T C C G G T A A1010
C T A T C G T C T T1020
G A G T C C A A C C1030
C G G T A G A C A C1040
G A C T T A T C G C1050
C A C T G G C A G C1060
A G C C A
C T G T A1070
A C A G G A T T A G1080
C A G A G C G A G G1090
T A T G T A G G C G1100
G T G C T A C A G A1110
G T T C T T G A A G1120
TG G T G G C C T A1130
A C T A
C G G C C T1140
A C C C T A G A G A1150
C A G T A T T T G G1160
T A T C T G G C G C1170
T C T G C T G A G T1180
C A G T A C C T T C1190
G A
A A G AG T T G1200
C T A G C T C T T G1210
A A T C G T C A A C1220
A A C C A C C G A C1230
T G G T T A G
G C G1240
G G G T GT G A A T1250
A
Page: 1 / 322.05.2019
Sequence: pcDNA3_rev_pcDNA3_rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
874687949.58
Samples:Bases:Average spacing:
12783100813.0
Average quality >= 10: 80, 20: 70, 30: 792
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G A C C T A G T G A10
C G G T G G T T T G20
T C C A A C T C A T30
C T A G A A G G C A40
C A G T C G A G G G50
A T C A C T C T C G60
G C A T G G A C C A70
T T T T A T G T T T80
C
A G G T T C A G G90
G G T C G T A A C A100
A C T C C G C C C G110
T C C A G C T C G A120
C C A G G A T G A G130
G T T A A G T A A A140
G C G T C T G A C C150
G T A A G T A G C A160
T C A C C
T T C T G170
G T C T T G T T A G180
A A T T T G T T A C190
C T T T T C G G T T200
A G A G C G G A T G210
T G T C G C G T T A220
A C G C T A G C A T230
G G A T C T C A C A240
T C A G A G A T
T T250
T G A G A C A C G G260
G C G G A T A T G T270
A T A T G G T G G T280
A A T G C T G A C C290
A A A C C T C T G G300
C G A A G A T G C G310
T C C G G C G T A G320
A T C C G G A T T A330
T T
C A T A C C G T340
C C C C C T C T A C350
A A A T G T G G T A360
T G G C T G A T T A370
T G A T C A G A T A380
G C A T G G C C T T390
T G C A G G G T A T400
C T T A T G G T A C410
T G T A A C T
G C T420
T T A T G T T T T T430
G G C G T C T T C C440
C T A G C A A A A T450
A G G C T G T C C C460
A A G A A C A T C G470
A T T T T C C A T G480
G C A G G C C T G A490
A C A C C A T A T C500
Page: 2 / 322.05.2019
Sequence: pcDNA3_rev_pcDNA3_rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
874687949.58
Samples:Bases:Average spacing:
12783100813.0
Average quality >= 10: 80, 20: 70, 30: 792
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C A T C C G C A G C510
T G A G A A T A T T520
G T A G G A G A T C530
A A A A T G A T A A540
C C A T C T C G C G550
G G T C C A A G T T560
T C C C T G C A T T570
A C T G G A T C T A580
T C A
A C A G G A G590
G G T C T A T A T A600
A G C A G A G C T G610
A A T C T G T G T G620
G G C A C T C G C T630
T C T G C G T T C T640
G A T T T A A T C T650
G C A T T T A G G T660
G A C A C T
A T A G670
A A T T A A C C C T680
C A C T A A A G G G690
T A A T A C G A C T700
C A C T A T A G G G710
A G A G C G G C T G720
T C T G G A T C T A730
C G T A A T A C G C740
T A G T T A T
T G C750
T C A G C G G T C C760
T T A T A C A T T A770
C G T C C T T T G T780
A G C T T T C T G G790
C A A G G T A G A C800
A A G C C C A T T G810
C T G A T T A G A G820
G A G T C
A A C C G830
T A G A A T C G A G840
A C C G A G G A G A850
G G G C A T T G G G860
T C A A C A G T A T870
A G A A C C G T G G880
A T G A T G T T G C890
C A C C T T T A T G900
T A T G T A C G G T910
T A C C T T G T A C920
G A C T T A C G G G930
C G G T G T G T G T940
A C A G A G T T T G950
A T C A T G A C T C960
AG C C G G
Page: 3 / 322.05.2019
Sequence: pcDNA3_rev_pcDNA3_rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
874687949.58
Samples:Bases:Average spacing:
12783100813.0
Average quality >= 10: 80, 20: 70, 30: 792
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G A G A970
G C T G A G A G T C980
A C T G A C G G G C990
T G C C C C C G C T1000
T C A A C T T
Page: 1 / 322.05.2019
Sequence: pCEP-Reverse_pCEP-ReverseClipped length:Left clip:Right clip:Avg. qual. in clip.:
8291484254.51
Samples:Bases:Average spacing:
12964101413.0
Average quality >= 10: 56, 20: 71, 30: 799
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C G CC T C G A G G10
G AT A C T C T C G20
G C A T G G A C C A30
T T T T A T G T T T40
C A G G T T C A G G50
G G T C G T A A C A60
A C T C C G C C C G70
T C C A G C T C G A80
C
C A G G A T G A G90
G T T A A G T A A A100
G C G T C T G A C C110
G T A A G T A G C A120
T C A C C T T C T G130
G T C T T G T T A G140
A A T T T G T T A C150
C T T T T C G G T T160
A G A G C
G G A T G170
T G T C G C G T T A180
A C G C T A G C A T190
G G A T C T C A C A200
T C A G A G A T T T210
T G A G A C A C G G220
G C G G A T A T G T230
A T A T G G T G G T240
A A T G C T G A C
C250
A A A C C T C T G G260
C G A A G A T G C G270
T C C G G C G T A G280
A T C C G G A T T A290
T T C A T A C C G T300
C C C C C T C T A C310
A A A T G T G G T A320
T G G C T G A T T A330
T G A
T C A G A T A340
G C A T G G C C T T350
T G C A G G G T A T360
C T T A T G G T A C370
T G T A A C T G C T380
T T A T G T T T T T390
G G C G T C T T C C400
C T A G C A A A A T410
A G G C T G T
C C C420
A A G A A C A T C G430
A T T T T C C A T G440
G C A G G C C T G A450
A C A C C A T A T C460
C A T C C G C A G C470
T G A G A A T A T T480
G T A G G A G A T C490
A A A A T G A T A A500
C
Page: 2 / 322.05.2019
Sequence: pCEP-Reverse_pCEP-ReverseClipped length:Left clip:Right clip:Avg. qual. in clip.:
8291484254.51
Samples:Bases:Average spacing:
12964101413.0
Average quality >= 10: 56, 20: 71, 30: 799
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C A T C T C G C G510
G G T C C A A G T T520
T C C C T G C A T T530
A C T G G A T C T A540
T C A A C A G G A G550
G G T C T A T A T A560
A G C A G A G C T G570
A A T C T G T G T G580
G G C A
C T C G C T590
T C T G C G T T C T600
G A T T T A A T C T610
G C A T T T A G G T620
G A C A C T A T A G630
A A T T A A C C C T640
C A C T A A A G G G650
T A A T A C G A C T660
C A C T A T
A G G G670
A G A G C G G C T G680
T C T G G A T C T A690
C G T A A T A C G C700
T A G T T A T T G C710
T C A G C G G T C C720
T T A T A C A T T A730
G G T C C T T T G T740
A G C T T T C
T G G750
C A A G G T A G A C760
A A G C C C A T T G770
C T G A T T A G A G780
G A G T C A A C C G790
T A G A A T C G A G800
A C C G A G G A G A810
G G G C A T T G G G820
T C A A C A
G T A T830
A G A A C C G T G G840
A T G A T G T T G C850
C A C C T T T A T G860
T A T G T A C G G T870
T A C C T T T G T T880
A C G A C T T A C G890
G G C G G T G T G T900
G
T A C A G A G T T910
T G A T C A T G G C920
T C A G C G G G A G930
A G T T G A G A G T940
T C A C T G A C G G950
G T G C C C G C T C960
A A C T T T
Page: 3 / 322.05.2019
Sequence: pCEP-Reverse_pCEP-ReverseClipped length:Left clip:Right clip:Avg. qual. in clip.:
8291484254.51
Samples:Bases:Average spacing:
12964101413.0
Average quality >= 10: 56, 20: 71, 30: 799
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C A G G970
A T A C G T T C T G980
G G T G C T G A T C990
G T T G A T A G T A1000
A T C T A AG A A G1010
C T A
Page: 1 / 322.05.2019
Sequence: pCR3.1-BGHrev_pCR31BGHrevClipped length:Left clip:Right clip:Avg. qual. in clip.:
8882090752.7
Samples:Bases:Average spacing:
12965101613.0
Average quality >= 10: 53, 20: 64, 30: 822
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A T T C G G A T G T10
A C T T T T A T G T20
T T A G G T T CA G30
G G G T C G T A A C40
A A C T C C G C C C50
G T C C A G C T C G60
A C C A G G A T G A70
G G T T A A G T A A80
A G C
G T C T G A C90
C G T A A G T A G C100
A T C A C C T T C T110
G G T C T T G T T A120
G A A T T T G T T A130
C C T T T T C G G T140
T A G A G C G G A T150
G T G T C G C G T T160
A A C G C T
A G C A170
T G G A T C T C A C180
A T C A G A G A T T190
T T G A G A C A C G200
G G C G G A T A T G210
T A T A T G G T G G220
T A A T G C T G A C230
C A A A C C T C T G240
G C G A A G A T G C250
G T C C G G C G T A260
G A T C C G G A T T270
A T T C A T A C C G280
T C C C C C T C T A290
C A A A T G T G G T300
A T G G C T G A T T310
A T G A T C A G A T320
A G C A T G G C C T330
T T G C A
G G G T A340
T C T T A T G G T A350
C T G T A A C T G C360
T T T A T G T T T T370
T G G C G T C T T C380
C C T A G C A A A A390
T A G G C T G T C C400
C A A G A A C A T C410
G A T T T T C C A
T420
G G C A G G C C T G430
A A C A C C A T A T440
C C A T C C G C A G450
C T G A G A A T A T460
T G T A G G A G A T470
C A A A A T G A T A480
A C C A T C T C G C490
G G G T C C A A G T500
T T C
Page: 2 / 322.05.2019
Sequence: pCR3.1-BGHrev_pCR31BGHrevClipped length:Left clip:Right clip:Avg. qual. in clip.:
8882090752.7
Samples:Bases:Average spacing:
12965101613.0
Average quality >= 10: 53, 20: 64, 30: 822
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C T G C A T510
T A C T G G A T C T520
A T C A A C A G G A530
G G G T C T A T A T540
A A G C A G A G C T550
G A A T C T G T G T560
G G G C A C T C G C570
T T C T G C G T T C580
T G A T T T
A A T C590
T G C A T T T A G G600
T G A C A C T A T A610
G A A T T A A C C C620
T C A C T A A A G G630
G T A A T A C G A C640
T C A C T A T A G G650
G A G A G C G G C T660
G T C T G G A T
C T670
A C G T A A T A C G680
C T A G T T A T T G690
C T C A G C G G T C700
C T T A T A C A T T710
A G G T C C T T T G720
T A G C T T T C T G730
G C A A G G T A G A740
C A A G C C C A T
T750
G C T G A T T A G A760
G G A G T C A A C C770
G T A G A A T C G A780
G A C C G A G G A G790
A G G G C A T T G G800
G T C A A C A G T A810
T A G A A C C G T G820
G A T G A T G T
T G830
C C A C C T T T A T840
G T A T G T A C G G850
T T A C C T T G T T860
A C G A C T T A C G870
G G C G G T G T G T880
G T A C A G A G T T890
T G A T C A T G G C900
T C A
G C G G G A G910
A G T T G A G A G G920
T C C A C T G A C G930
G G T G C C C C G C940
T C A C C T T C A G950
A T A C G G T C T G960
G G T G C T G C
Page: 3 / 322.05.2019
Sequence: pCR3.1-BGHrev_pCR31BGHrevClipped length:Left clip:Right clip:Avg. qual. in clip.:
8882090752.7
Samples:Bases:Average spacing:
12965101613.0
Average quality >= 10: 53, 20: 64, 30: 822
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T C970
G T GA T T G T A T980
C T A G A G C C T A990
G C G C G T C T G T1000
G T G A A A T T T G1010
C T A A T
Page: 1 / 322.05.2019
Sequence: pEX-For_pEX-For Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8971290852.35
Samples:Bases:Average spacing:
12962102013.0
Average quality >= 10: 58, 20: 63, 30: 820
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C A T C GG A C C10
G GG TG T G G C G20
G G T G T C G G G G30
C T G G C T T A A C40
T A T G C G G C A T50
C A G A G C A G A T60
T G T A C T G A G A70
G T G C A C C A T A80
T G G
T C G A C C T90
C G A G T T A A T T100
A A C G T A T A C G110
G T T A C C T T G T120
T A C G A C T T G C130
A A A T G G C A T T140
C T G A C A T C C G150
A C T G G T T C C A160
A T T G A C A
A G C170
T A C T A T T G C C180
A G C A T T G C T G190
C C G C A A A T G G200
G C G G T A G G C G210
T G C G C C A T C C220
A C G C T G T T T T230
G C T C T A G C G C240
C A C C A T G A A A250
C
A G G C A G C A G260
C C A C A G G T A A270
G A T T G C T G T T280
G C A C A G T G A T290
T C G G G A T G T T300
T A A T A C C A C T310
A C A A G A A A T T320
G A G A T G G T G C330
A C T A C
C A C T A340
C A A T G G A T G T350
C A T C G G A A G A360
G A G T A G T T T T370
T C A C T T C A C A380
G G T C G A T A A G390
C A T G C G T A G G400
A G A A A G A G T C410
T T A A G T A A T C420
T A G A T A A C G A430
G C C C A G C A A C440
A C C A A G G A T T450
A G G A C A A G G C460
T G G T G G T G A G470
T T T C G T C A C C480
A G T A C A G G A A490
A C A G C T A T G A500
C C T
Page: 2 / 322.05.2019
Sequence: pEX-For_pEX-For Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8971290852.35
Samples:Bases:Average spacing:
12962102013.0
Average quality >= 10: 58, 20: 63, 30: 820
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G T A A A A C510
G A C G G C C A G T520
A G G G T T T T C C530
C A G T C A C G A C540
G T T G G T C G T C550
A G A C T G T C G A560
T G A A G C C C G T570
T C G A C C C C G C580
C T C G A T
C C G G590
C T A A C T A G A G600
A A C C C A C T G G610
G C A A C T A G A A620
G G C A C A G T C A630
G A G C T C G T T T640
A G T G A A C C G G650
T G G T T T G T C C660
A A A C T C A T
C T670
A G A A G G C A C A680
G T C G A G G G A T690
C A C T C T C G G C700
A T G G A C C A T T710
T T A T G T T T C A720
G G T T C A G G G G730
T C G T A A C A A C740
T C C G C C C G
T C750
C A G C T C G A C C760
A G G A T G A G G T770
T A A G T A A A G C780
G T C T G A C C G T790
A A G T A G C A T C800
A C C T T C T G G T810
C T T G T T A G A A820
T T T G T T A
C C T830
T T T C G G T T A G840
A G C G G A T G T G850
T C G C G T T A A C860
G C T A G C A T G G870
A T C T C A C A T C880
A G A G A T T T T G890
A G A C A C G G G C900
G G A T A T G T A T910
A T G G T G G T A A920
T G C T G A C C A A930
A C C T C T G G G C940
G G A A G A T G C G950
T C G G C G T A G A960
T C C G G G A
Page: 3 / 322.05.2019
Sequence: pEX-For_pEX-For Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8971290852.35
Samples:Bases:Average spacing:
12962102013.0
Average quality >= 10: 58, 20: 63, 30: 820
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T A A970
T C A T T A C G T C980
C C C C T C T A C A990
A A T G T G G T A T1000
G G A C T G A A T T1010
A T T G A T C A G
Page: 1 / 322.05.2019
Sequence: pEX-Rev_pEX-Rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8961090552.3
Samples:Bases:Average spacing:
12580100913.0
Average quality >= 10: 66, 20: 72, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G C A T G TT G T G10
T G G A T T G T G A20
G C G G AT A A C A30
A T T T C A C A C A40
G G A A T T C G C T50
A G C T C T A G A T60
T A A T T A A C C A70
C G A A C A G C A C80
C C
A G G A C C G T90
A T C C T G A A G T100
T G A G C G G G G C110
A C C C G T C A G T120
G G A C C T C T C A130
A C T C T C C C G C140
T G A G C C A T G A150
T C A A A C T C T G160
T A C A C
A C A C C170
G C C C G T A A G T180
C G T A A C A A G G190
T A A C C G T A C A200
T A C A T A A A G G210
T G G C A A C A T C220
A T C C A C G G T T230
C T A T A C T G T T240
G A C C C A A T G
C250
C C T C T C C T C G260
G T C T C G A T T C270
T A C G G T T G A C280
T C C T C T A A T C290
A G C A A T G G G C300
T T G T C T A C C T310
T G C C A G A A A G320
C T A C A A A G G A330
C C T
A A T G T A T340
A A G G A C C G C T350
G A G C A A T A A C360
T A G C G T A T T A370
C G T A G A T C C A380
G A C A G C C G C T390
C T C C C T A T A G400
T G A G T C G T A T410
T A C C C T T
T A G420
T G A G G G T T A A430
T T C T A T A G T G440
T C A C C T A A A T450
G C A G A T T A A A460
T C A G A A C G C A470
G A A G C G A G T G480
C C C A C A C A G A490
T T C A G C T C T G500
C
Page: 2 / 322.05.2019
Sequence: pEX-Rev_pEX-Rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8961090552.3
Samples:Bases:Average spacing:
12580100913.0
Average quality >= 10: 66, 20: 72, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T T A T A T A G A510
C C C T C C T G T T520
G A T A G A T C C A530
G T A A T G C A G G540
G A A A C T T G G A550
C C C G C G A G A T560
G G T T A T C A T T570
T T G A T C T C C T580
A C A A
T A T T C T590
C A G C T G C G G A600
T G G A T A T G G T610
G T T C A G G C C T620
G C C A T G G A A A630
A T C G A T G T T C640
T T G G G A C A G C650
C T A T T T T G C T660
A G G G A A
G A C G670
C C A A A A A C A T680
A A A G C A G T T A690
C A G T A C C A T A700
A G A T A C C C T G710
C A A A G G C C A T720
G C T A T C T G A T730
C A T A A T C A G C740
C A T A C C A C A
T750
T T G T A G A G G G760
G G A C G G T A T G770
A A T A A T C C G G780
A T C T A C G C C G790
G A C G C A T C T T800
C G C C A G A G G T810
T T G G T C A G C A820
T T A C C A C C A
T830
A T A C A T A T C C840
G C C C G T G T C T850
C A A A A T C T C T860
G A T G T G A G A T870
C C A T G C T A G C880
G T T A A C G C G A890
C A C A T C C G C T900
C T A A C C
G A A A910
A G G T A C A A T T920
C T A C A G A C C A930
G A T G T G A T G C940
T A C T T A C G G T950
C A G A C G C T T T960
A C T T A C C T C A970
Page: 3 / 322.05.2019
Sequence: pEX-Rev_pEX-Rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8961090552.3
Samples:Bases:Average spacing:
12580100913.0
Average quality >= 10: 66, 20: 72, 30: 819
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T C C T G G T C G A980
G C T G G G A C G G990
G G G C G G G G A G1000
T T G T T A C G
Page: 1 / 322.05.2019
Sequence: pJET1.2for_pJET12for Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9021491552.51
Samples:Bases:Average spacing:
12961102813.0
Average quality >= 10: 68, 20: 65, 30: 815
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G G T T T T T G G A10
C T C G T A A A C G20
C T A G T T A T T G30
C T C A G C G G T C40
C T T A T A C A T T50
A G G T C C T T T G60
T A G C T T T C T G70
G C A A G G T A G A80
C A A G C
C C A T T90
G C T G A T T A G A100
G G A G T C A A C C110
G T A G A A T C G A120
G A C C G A G G A G130
A G G G C A T T G G140
G T C A A C A G T A150
T A G A A C C G T G160
G A T G A T G T T
G170
C C A C C T T T A T180
G T A T G T A C G G190
T T A C C T T G T T200
A C G A C T T A C G210
G G C G G T G T G T220
G T A C A G A G T T230
T G A T C A T G G C240
T C A G C G G G A G250
A G
T T G A G A G G260
T C C A C T G A C G270
G G T G C C C C G C280
T C A A C T T C A G290
G A T A C G G T C C300
T G G G T G C T G T310
T C G T G G T T A A320
T T A A T C T A G A330
G C T A G C G
A A T340
T C C T G T G T G A350
A A T T G T T A T C360
C G C T C A C A A T370
T C C A C A C A A C380
A T A C G A G C C G390
G A A G C A T A A A400
G T G T A A A G C C410
T G G G G T G C C T420
A
A T G A G T G A G430
C T A A C T C A C A440
T T A A T T G C G T450
T G C G C T C A C T460
G C C C G C T T T C470
C A G T C G G G A A480
A C C T G T C G T G490
C C A G C T G C A T500
T A A T G
Page: 2 / 322.05.2019
Sequence: pJET1.2for_pJET12for Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9021491552.51
Samples:Bases:Average spacing:
12961102813.0
Average quality >= 10: 68, 20: 65, 30: 815
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A A T C G510
G C C A A C G C G C520
G G G G A G A G G C530
G G T T T G C G T A540
T T G G G C G C T C550
T T C C G C T T C C560
T C G C T C A C T G570
A C T C G C T G C G580
C T C G G T C G
T T590
C G G C T G C G G C600
G A G C G G T A T C610
A G C T C A C T C A620
A A G G C G G T A A630
T A C G G T T A T C640
C A C A G A A T C A650
G G G G A T A A C G660
C A G G A A A G A A670
C
A T G T G A G C A680
A A A G G C C A G C690
A A A A G G C C A G700
G A A C C G T A A A710
A G G C C G C G T T720
G C T G G C G T T T730
T T C C A T A G G C740
T C C G C C C C C C750
T G
A C G A G C A T760
C A C A A A A A T C770
G A C G C T C A A G780
T C A G A G G T G G790
C G A A A C C C G A800
C A G G A C T A T A810
A A G A T A C C A G820
G C G T T T C C C C830
C T G
A A A G C T C840
C C T C G T G C G C850
T C T C C T G T T C860
C G A C C C T G C C870
G C T T A C C G G A880
T A C C T G T C C G890
C C T T T C T C C C900
T T C G G G A A G C910
G T
G C G C T T C T920
C A T A G C T C A C930
G C T G T A G T A T940
C T C A G T T C G G950
T G T A G T C G T C960
G C T C A G C T G G970
C T G T G
Page: 3 / 322.05.2019
Sequence: pJET1.2for_pJET12for Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9021491552.51
Samples:Bases:Average spacing:
12961102813.0
Average quality >= 10: 68, 20: 65, 30: 815
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T G C A C980
G A C C C C C G G T990
C A G C C C G A C G1000
C T G C G C C T T A1010
T C C G T A A C C T1020
A A T C G T C
Page: 1 / 322.05.2019
Sequence: pJET1.2rev_pJET12rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8711188152.71
Samples:Bases:Average spacing:
12964101513.0
Average quality >= 10: 64, 20: 62, 30: 817
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C G G T A C G TA10
T C C T C C G C A G20
C T G A G A T A T T30
G T A G G A G A T C40
A A A A T G A T A A50
C C A T C T C G C G60
G G T C C A A G T T70
T C C C T G C A T T80
A
C T G G A T C T A90
T C A A C A G G A G100
G G T C T A T A T A110
A G C A G A G C T G120
A A T C T G T G T G130
G G C A C T C G C T140
T C T G C G T T C T150
G A T T T A A T C T160
G C A T T
T A G G T170
G A C A C T A T A G180
A A T T A A C C C T190
C A C T A A A G G G200
T A A T A C G A C T210
C A C T A T A G G G220
A G A G C G G C T G230
T C T G G A T C T A240
C G T A A T A C G
C250
T A G T T A T T G C260
T C A G C G G T C C270
T T A T A C A T T A280
G G T C C T T T G T290
A G C T T T C T G G300
C A A G G T A G A C310
A A G C C C A T T G320
C T G A T T A G A G330
G A G
T C A A C C G340
T A G A A T C G A G350
A C C G A G G A G A360
G G G C A T T G G G370
T C A A C A G T A T380
A G A A C C G T G G390
A T G A T G T T G C400
C A C C T T T A T G410
T A T G T A C
G G T420
T A C C T T G T T A430
C G A C T T A C G G440
G C G G T G T G T G450
T A C A G A G T T T460
G A T C A T G G C T470
C A G C G G G A G A480
G T T G A G A G G T490
C C A C T G A C G G500
Page: 2 / 322.05.2019
Sequence: pJET1.2rev_pJET12rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8711188152.71
Samples:Bases:Average spacing:
12964101513.0
Average quality >= 10: 64, 20: 62, 30: 817
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G T G C C C C G C T510
C A A C T T C A G G520
A T A C G G T C C T530
G G G T G C T G T T540
C G T G G T T A A T550
T A A T C T A G A G560
C T A G C G A A T T570
C C T G T G T G A A580
A T T
G T T A T C C590
G C T C A C A A T T600
C C A C A C A A C A610
T A C G A G C C G G620
A A G C A T A A A G630
T G T A A A G C C T640
G G G G T G C C T A650
A T G A G T G A G C660
T A A C T C
A C A T670
T A A T T G C G T T680
G C G C T C A C T G690
C C C G C T T T C C700
A G T C G G G A A C710
C T G T C G T G C C720
A G C T G C A T T A730
A T G A A T C G G C740
C A A C G C
G C G G750
G G A G A G G C G G760
T T T G C G T A T T770
G G G C G C T C T T780
C C G C T T C C T C790
G C T C A C T G A C800
T C G C T G C G C T810
C G G T C G T T C G820
G C T G
C G G C G A830
G C G G T A T C A G840
C T C A C T C A A G850
G C G G T A A T A C860
G G T T A T C C A C870
A G A A T C A G G G880
A T A A C G C A G A890
A G A A C A
T G T G900
A G C A A A G G C C910
A G C A A A A G G C920
C A G G A A C C G T930
A A A A A G C C G C940
G T T G C T G G C G950
T T T T T C C A T A960
G G
Page: 3 / 322.05.2019
Sequence: pJET1.2rev_pJET12rev Clipped length:Left clip:Right clip:Avg. qual. in clip.:
8711188152.71
Samples:Bases:Average spacing:
12964101513.0
Average quality >= 10: 64, 20: 62, 30: 817
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C T C C G C C C970
C C T G A C G A G C980
A T C A C A A A A A990
A A T C G A C C G C1000
C T C A A G T T C A1010
A A A G
Page: 1 / 322.05.2019
Sequence: pLKO1_pLKO1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101612102751.75
Samples:Bases:Average spacing:
16303123014.0
Average quality >= 10: 106, 20: 83, 30: 935
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C T G C A T AC GG10
C TG C C G C T C A20
C T T CA G G A T A30
C G G T C C T G G G40
T G C T G T T C G T50
G G T T A A T T A A60
T C T A G A G C T A70
G C G A A T T C C
T80
G T G T G A A A T T90
G T A T C G C G G G100
T G G C A G C T C T110
A G A G C T A G C G120
A A T T C T T T G G130
T G A A A T T G T T140
A T C C G C T C A C150
A A T T C C A C A C160
A A C
A T A C G A G170
C C G G A A G C A T180
A A A G T G T A A A190
G C C T G G G G T G200
C C T A A T G A G T210
G A G C T A A C T C220
A C A T T A A T T G230
C G T T G C G C T C240
A C T G C C
C G C T250
T T C C A G T C G G260
G A A A C C T G T C270
G T G C C A G C T G280
C A T T A A T G A A290
T C G G C C A A C G300
C G C G G G G A G A310
G G C G G T T T G C320
G T A T T G G G C
G330
C T C T T C C G C T340
T C C T C G C T C A350
C T G A C T C G C T360
G C G C T C G G T C370
G T T C G G C T G C380
G G C G A G C G G T390
A T C A G C T C A C400
T C A A A G G C G G410
T A A
T A C G G T T420
A T C C A C A G A A430
T C A G G G G A T A440
A C G C A G G A A A450
G A A C A T G T G A460
G C A A A A G G C C470
A G C A A A A G G C480
C A G G A A C C G T490
A A A A A G
Page: 2 / 322.05.2019
Sequence: pLKO1_pLKO1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101612102751.75
Samples:Bases:Average spacing:
16303123014.0
Average quality >= 10: 106, 20: 83, 30: 935
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G C C G500
C G T T G C T G G C510
G T T T T T C C A T520
A G G C T C C G C C530
C C C C T G A C G A540
G C A T C A C A A A550
A A T C G A C G C T560
C A A G T C A G A G570
G T G G C G A A A C580
C C G A C A G G A C590
T A T A A A G A T A600
C C A G G C G T T T610
C C C C C T G G A A620
G C T C C C T C G T630
G C G C T C T C C T640
G T T C C G A C C C650
T G C C G C T T A C660
C G G
A T A C C T G670
T C C G C C T T T C680
T C C C T T C G G G690
A A G C G T G G C G700
C T T T C T C A T A710
G C T C A C G C T G720
T A G G T A T C T C730
A G T T C G G T G T740
A G G T
C G T T C G750
C T C C A A G C T G760
G G C T G T G T G C770
A C G A A C C C C C780
C G T T C A G C C C790
G A C C G C T G C G800
C C T T A T C C G G810
T A A C T A T C G T820
C T T G A
G T C C A830
A C C C G G T A A G840
A C A C G A C T T A850
T C G C C A C T G G860
C A G C A G C C A C870
T G G T A A C A G G880
A T T A G C A G A G890
C G A G G T A T G T900
A G G C
G G T G C T910
A C A G A G T T C T920
T G A A G T G G T G930
G C C T A A C T A C940
G G C T A C A C T A950
G A A G A A C A G T960
A T T T G G T A T C970
T G C G C T C T G C980
T G
Page: 3 / 322.05.2019
Sequence: pLKO1_pLKO1 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
101612102751.75
Samples:Bases:Average spacing:
16303123014.0
Average quality >= 10: 106, 20: 83, 30: 935
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
A A G C C A G T990
T A C C T T T C G G1000
A A A A A G G A G T1010
T G G T A G C T C T1020
T G A T C C G G C A1030
A A C A A A C C A C1040
C G C T G G T A G C1050
G G T G G T T
T T T1060
T T T G T T T G C A1070
A G C A G C A G A T1080
T A C G C G C A G A1090
A A A A A A G A T C1100
T C A G A G A T C C1110
T T T G A T C T T T1120
T T C T
A C G G G G1130
T C T G A C G C C T1140
C A G TG G A C G A1150
A A C T C A C G T A1160
G C A T T T G T T C1170
A T G A A
T A T C A1180
A A C G G A T C T C1190
A C T A G A T C T T1200
T T A A A T T A A A1210
A A TG G G A A
G G1220
T T T T A A A A T
Page: 1 / 322.05.2019
Sequence: T7 term_T7term Clipped length:Left clip:Right clip:Avg. qual. in clip.:
860586453.31
Samples:Bases:Average spacing:
12787100713.0
Average quality >= 10: 56, 20: 67, 30: 810
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C T GG T C T T G T10
A G C T T T C T G G20
C A G G T A G A C A30
A G C C C A T T G C40
T G A T T A G A G G50
A G T C A A C C G T60
A G A A T C G A G A70
C C G A G G A G A
G80
G G C A T T G G G T90
C A A C A G T A T A100
G A A C C G T G G A110
T G A T G T T G C C120
A C C T T T A T G T130
A T G T A C G G T T140
A C C T T G T T A C150
G A C T T A C G G G160
C G
G T G T G T G T170
A C A G A G T T T G180
A T C A T G G C T C190
A G C G G G A G A G200
T T G A G A G G T C210
C A C T G A C G G G220
T G C C C C G C T C230
A A C T T C A G G A240
T A C G G T
C C T G250
G G T G C T G T T C260
G T G G T T A A T T270
A A T C T A G A G C280
T A G C G A A T T C290
C T G T G T G A A A300
T T G T T A T C C G310
C T C A C A A T T C320
C A C A C A A C A T330
A
C G A G C C G G A340
A G C A T A A A G T350
G T A A A G C C T G360
G G G T G C C T A A370
T G A G T G A G C T380
A A C T C A C A T T390
A A T T G C G T T G400
C G C T C A C T G C410
C C G C T
T T C C A420
G T C G G G A A A C430
C T G T C G T G C C440
A G C T G C A T T A450
A T G A A T C G G C460
C A A C G C G C G G470
G G A G A G G C G G480
T T T G C G T A T T490
G G G C G C T C
Page: 2 / 322.05.2019
Sequence: T7 term_T7term Clipped length:Left clip:Right clip:Avg. qual. in clip.:
860586453.31
Samples:Bases:Average spacing:
12787100713.0
Average quality >= 10: 56, 20: 67, 30: 810
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T T500
C C G C T T C C T C510
G C T C A C T G A C520
T C G C T G C G C T530
C G G T C G T T C G540
G C T G C G G C G A550
G C G G T A T C A G560
C T C A C T C A A A570
G G C G G T A A T A580
C
G G T T A T C C A590
C A G A A T C A G G600
G G A T A A C G C A610
G G A A A G A A C A620
T G T G A G C A A A630
A G G C C A G C A A640
A A G G C C A G G A650
A C C G T A A A A A660
G G C C
G C G T T G670
C T G G C G T T T T680
T C C A T A G G C T690
C C G C C C C C C T700
G A C G A G C A T C710
A C A A A A A T C G720
A C G C T C A A G T730
C A G A G G T G G C740
G A A A C C
C G A C750
A G G A C T A T A A760
A G A T A C C A G G770
C G T T T C C C C C780
T G T A A G C T C C790
C T C G T G C G C T800
C T C C T G T T C C810
G A C C C T G C C G820
C T T A C C
G G A T830
A C C T G T C C G C840
C T T T C T C C C T850
T C G G G A A G C G860
T G C G C T T T C T870
C A T A G C T C A C880
G C T G T A G T A T890
C T C A G T T C G G900
T G T A G T C G T T910
C G C T C C A G C T920
G G G C T G T G T G930
C A C G A A C C C C940
C C G T C A G C C C950
G A C G G C T G C G960
C T A
Page: 3 / 322.05.2019
Sequence: T7 term_T7term Clipped length:Left clip:Right clip:Avg. qual. in clip.:
860586453.31
Samples:Bases:Average spacing:
12787100713.0
Average quality >= 10: 56, 20: 67, 30: 810
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T C G G T A C970
T A T C G T C T T G980
A G T C C A A C C C990
G G T A A G A A A C1000
A C G A A C
Page: 1 / 322.05.2019
Sequence: T7_T7 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9061491952.74
Samples:Bases:Average spacing:
12827101813.0
Average quality >= 10: 62, 20: 59, 30: 827
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C C C C A T T C TG10
G AT C A C G T A T20
A C G C T A G T T A30
T T G C T C A G C G40
G T C C T T A T A C50
A T T A G G T C C T60
T T G T A G C T T T70
C T G G C A A G G T80
A G A C
A A G C C C90
A T T G C T G A T T100
A G A G G A G T C A110
A C C G T A G A A T120
C G A G A C C G A G130
G A G A G G G C A T140
T G G G T C A A C A150
G T A T A G A A C C160
G T G G A T G
A T G170
T T G C C A C C T T180
T A T G T A T G T A190
C G G T T A C C T T200
G T T A C G A C T T210
A C G G G C G G T G220
T G T G T A C A G A230
G T T T G A T C A T240
G G C T C A G C G G250
G
A G A G T T G A G260
A G G T C C A C T G270
A C G G G T G C C C280
C G C T C A A C T T290
C A G G A T A C G G300
T C C T G G G T G C310
T G T T C G T G G T320
T A A T T A A T C T330
A G A G C
T A G C G340
A A T T C C T G T G350
T G A A A T T G T T360
A T C C G C T C A C370
A A T T C C A C A C380
A A C A T A C G A G390
C C G G A A G C A T400
A A A G T G T A A A410
G C C T G G G G T
G420
C C T A A T G A G T430
G A G C T A A C T C440
A C A T T A A T T G450
C G T T G C G C T C460
A C T G C C C G C T470
T T C C A G T C G G480
G A A A C C T G T C490
G T G C C A G C T G500
C A T
Page: 2 / 322.05.2019
Sequence: T7_T7 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9061491952.74
Samples:Bases:Average spacing:
12827101813.0
Average quality >= 10: 62, 20: 59, 30: 827
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
T A A T G A A510
T C G G C C A A C G520
C G C G G G G A G A530
G G C G G T T T G C540
G T A T T G G G C G550
C T C T T C C G C T560
T C C T C G C T C A570
C T G A C T C G C T580
G C G C T C
G G T C590
G T T C G G C T G C600
G G C G A G C G G T610
A T C A G C T C A C620
T C A A A G G C G G630
T A A T A C G G T T640
A T C C A C A G A A650
T C A G G G G A T A660
A C G C A G G A A
A670
G A A C A T G T G A680
G C A A A A G G C C690
A G C A A A A G G C700
C A G G A A C C G T710
A A A A A G G C C G720
C G T T G C T G G C730
G T T T T T C C A T740
A G G C T C C G C C750
C
C C C T G A C G A760
G C A T C A C A A A770
A A T C G A C G C T780
C A A G T C A G A G790
G T G G C G A A A C800
C C G A C A G G A C810
T A T A A A G A T A820
C C A G G C G T T T830
C C
C C C T G G A A840
G C T C C C T C G T850
G C G C T C T C C T860
G T T C C G A C C C870
T G C C G C T T A C880
C G G A T A C C T G890
T C C G C C C T T T900
C T C C C T T T C G910
G G A A G C G T G C920
G C T T C T C A T A930
G C T C A C G C T G940
T A G T A T C T C A950
G T C G T G T A G T960
C G G T C G C T C A970
G
Page: 3 / 322.05.2019
Sequence: T7_T7 Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9061491952.74
Samples:Bases:Average spacing:
12827101813.0
Average quality >= 10: 62, 20: 59, 30: 827
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C T G G G C T G T980
G T G C A C G A A C990
C C C C C C G T T C1000
A G C C C G G A C C1010
G G C TG G C
Page: 1 / 322.05.2019
Sequence: U6_fwd_U6_fwd Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9511196149.51
Samples:Bases:Average spacing:
16301121614.0
Average quality >= 10: 115, 20: 98, 30: 857
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
GC C G T C T C T G10
A T C C G A C T G G20
T T C A T T G A C A30
G C T A C T A T T G40
C C A G C A T T G C50
T G C C G C A A A T60
G G G C G G T A G G70
C G T G C G C C
A T80
C C A C G C T G T T90
T T G C T C T A G C100
G C C A C C A T G A110
A A C A G G C A G G120
T T G C A C A G T G130
A T T C G G G A T G140
T T T A A T A C C A150
C T A C A A G A A A160
T T
G A G A T G G T170
G C A C T A C C A C180
T A C A A T G G A T190
G T C A T C G G A A200
G A G A G T A G T T210
T T T C A C T T C A220
C A G G T C G A T A230
A G C A T G C G T A240
G G A G A A
A G A G250
T C T T A A G T A A260
T C T A G A T A A C270
G A G C C C A G C A280
A C A C C A A G T G290
A G T T T C G T C A300
C C A G T A C A G G310
A A A C A G C T A T320
G A C C T G T A A A330
A C G A C G G C C A340
G T A G G G T T T T350
C C C A G T C A C G360
A C G T T G G T C G370
T C A G A C T G T C380
G A T G A A G C C C390
G T T C G A C C C C400
G C C T C G A T C C410
G G C T
A A C T A G420
A G A A C C C A C T430
G G G C A A C T A G440
A A G T C A C A G T450
C A G A G C T C G T460
T T A G T G A A C C470
G G T G G T T T G T480
C C A A A C T C A T490
C T A G A A G G
Page: 2 / 322.05.2019
Sequence: U6_fwd_U6_fwd Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9511196149.51
Samples:Bases:Average spacing:
16301121614.0
Average quality >= 10: 115, 20: 98, 30: 857
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
C A500
C A G T C G A G G G510
A T C A C T C T C G520
G A C C C T A A C T530
G A C A C A C A T T540
C C C A T G G A C C550
A T T T T A T G T T560
T C A G G T T C A G570
G G G T C G T A A C580
A
A C T C C G C C C590
G T C C A G C T C G600
A C C A G G A T G A610
G G T T A A G T A A620
A G C G T C T G A C630
C G T A A G T A G C640
A T C A C C T T C T650
G G T C T T G T T A660
G A A T
T T G T T A670
C C T T T T C G G T680
T A G A G C G G A T690
G T G T C G C G T T700
A A C G C T A G C A710
T G G A T C T C A C720
A G A T T A T G C G730
G C C G T G T A C A740
A T C A G
A G A T T750
T T G A G A C A C G760
G G C G G A T A T G770
T A T A T G G T G G780
T A A T G C T G A C790
C A A A C C T C T G800
G C G A A G A T G C810
G T C C G G C G T A820
G A T C C G
G A T T830
A T T C A T A C C G840
T C C C C C T C T A850
C A A A T G T G G T860
A T G G C T G A T T870
A T G A T C A G A T880
A G C A T G G C C T890
T T G C A G G G T A900
T C T T A T G
G T A910
C T G T A A C T G C920
T T T A T G T T T T930
T G G C G T C T T C940
C C T A G C A A A A950
T A G C T G T C C C960
A A G A A C A T C G970
A T T T T C C A T G980
G C A
Page: 3 / 322.05.2019
Sequence: U6_fwd_U6_fwd Clipped length:Left clip:Right clip:Avg. qual. in clip.:
9511196149.51
Samples:Bases:Average spacing:
16301121614.0
Average quality >= 10: 115, 20: 98, 30: 857
Clip. 1 BQ 20 WL 10 Quality: 0 - 910 - 1920 - 29>= 30
G C C T G A C990
A C C A T A T C C A1000
T C C G C A G C T G1010
A G A A T A T T T G1020
T A G A G A T C A A1030
T G A T A C A T C T1040
C G C G G G T C C A1050
G T
T C C C T G C A1060
A T A C T G G A T C1070
T A T C A A C A G G1080
A G G G T C T A T A1090
T A G C A G A G C T1100
G A T C T G T G T G1110
G C A C T C G C
T C1120
T G C G T C T G A T1130
A T C T G C A T T A1140
G T G A C C T A T G1150
A C T A C C T A C T1160
A C G G
T A A T C G1170
A T C A C A T A T A1180
C G A A G C G C T G1190
T C T G G A A TC T1200
A C G T A
T A C G C1210
C T A A G