christopher j. easley, ph.d. knowles associate professor auburn university chemistry and...
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Christopher J. Easley, Ph.D.Knowles Associate Professor
Auburn UniversityChemistry and Biochemistry
Auburn, AL, USA
Auburn University Cluster Hire InitiativeSession #5: Nano-Bio Sciences
Auburn University, Auburn, AL, USA
December 1, 2014
Opportunities in Measurement Science and
Chemical Biology using Nanoscale Assemblies
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Diabetes in the US (2004 – 2010)
29.1 million in U.S. (9.3%) $245 billion in 2012 = 8 × NIH
budget Related to heart disease,
Alzheimer’s, stroke, blindness, kidney disease, nervous system disease, …
Centers for Disease Control (CDC), National Diabetes Statistics Report 2014, Atlanta, GA: U.S. Dept. of Health and Human Services.
Sarah Illenberger
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Godwin, L. A.; Brooks, J. C.; Hoepfner, L. D.; Wanders, D.; Judd, R. L.; Easley, C. J., Analyst 2014, DOI: 10.1039/C4AN01725K.
Microfluidic Methods for Secretion Sampling from Primary Cells
• Collaboration with Judd group (AU Vet Med, AP&P)• Boshell Diabetes and Metabolic Diseases Research Program
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Nanoliter-Scale Protein Assays
Before After
100
150
200
0 5 10 15 20 25Time (s)
8-b
it R
ed
Inte
nsi
ty
Negou, J.; Li, X.; Kim, J.; Hu, J.; Easley, C. J., 2014, in preparation.
Detection of 300 attomoles of
protein in ~10 minutes with direct
fluorescence
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Rapid Protein Assays in Undiluted Human Serum
• Developed in collaboration with Shannon lab (AU Chem & Biochem)
• Additional collaborations with Simonian lab (AU Mat. Eng.) and Greene lab (AU Nutrition)Hu, J.; Wang, T.; Kim, J.; Shannon, C.; Easley, C. J., J. Am. Chem. Soc. 2012, 134, 7066–
7072.Hu, J.; Shannon, C.; Easley, C. J.; et al., J. Am. Chem. Soc. 2014, 136, 8467-8474.
AU-IGP
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Nano-Bio Expertise in Chem. & Biochem. Dept.
Imaging: Goldsmith (MRI), Easley (fluorescence), Shannon
(Raman), Zhan (electrochemical)
Enzymology: Duin, Ellis, Goodwin, Mansoorabadi
Synthetic Chemistry: Merner (natural products, nanotubes), Schneller (antivirals),
Goldsmith (MRI probes), Gorden (sensors)
Computational: Acevedo, Patkowski,
Ortiz
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Expertise Needed at Auburn University
• Specialties with room to grow• Imaging
• Nanoparticle-mediated gene regulation & imaging• Super-resolution fluoresecence microscopy• Two-photon imaging and probe synthesis• MRI imaging probes and computational analysis
• Mass Spectrometry• Proteomics, metabolomics
• General Note on Hiring• We should improve sharing of faculty interview
schedules. How can this be done?
[email protected] Cluster Hire Initiative, Nano-Bio Sciences December 1, 2014
Nano-Bio Overlap at AU (instrumentation & programs)
AU MRI Research Cente
r
Boshell Diabetes Progr
am
AURIC
Cluster Hires: Health
Sciences
Cluster Hires:
Computational Science
CMB Program
AMIL I & AMIL II Metabolic Phenotyping
Lab
Flow Cytometry Lab
*
Mass Spectrometry Cen
ter
*NMR Center *
Confocal Raman Imagin
g*Microspectrophotomet
ry
*X-Ray Crystallography *EPR Spectroscopy
*Fluorescence
Microscopy
* Housed within the Chemistry Building