christine e. kasper, phd, rn, faan dept. of veterans affairs &
DESCRIPTION
Neurobehavioral Impairment is Prevented by Acute Minocycline Treatment in a Rodent Model of mild Blast Traumatic Brain Injury. Christine E. Kasper, PhD, RN, FAAN Dept. of Veterans Affairs & Professor, USUHS Graduate School of Nursing. Aim. - PowerPoint PPT PresentationTRANSCRIPT
Neurobehavioral Impairment is Prevented by Acute Minocycline Treatment in a Rodent Model of
mild Blast Traumatic Brain Injury
Christine E. Kasper, PhD, RN, FAAN
Dept. of Veterans Affairs &Professor, USUHS Graduate School of
Nursing
Aim To determine the effect of acute
treatment with minocycline, an FDA approved non-steroidal anti-inflammatory drug, on the functional and molecular outcomes of mild blast TBI.
HypothesisFor dorsal and ventral hippocampus:
Anti-inflammatory treatment with minocycline will improve the functional outcome in mbTBI
Anti-inflammatory treatment with minocycline will decrease cell apoptosis and reduce neural inflammation
Blast Injury
Method
245-265 g male Sprague Dawley Rats • N = 32
Normal housing (2/cage) 4 Groups:
• Sham saline treated N = 8
• Sham mincocycline treated N = 8• Blast injured saline treated N =
8• Blast injured minocycline treated N = 8
Timeline
Behavioral Testing
8 and 45 days post-bTBI:• Locomotion: Open Field (OF)• Anxiety: Elevated Plus Maze (EPM)• Spatial Learning & Memory: Barnes Maze
(BM)
Open Field
Anxiety: EPM
Spatial Learning & Memory: BM
Immunohistochemistry
GFAP: CNS glia – Green
Caspase 3: Cell apoptosis – Red
DAPI: Cell nuclei – Blue
Ventral HippocampusGFAP/Cas3, 10x
a. Control Minocycline b. Control bTBc. bTBI Minocycline
a
b
c
Ventral HippocampusGFAP/Cas3, 60x
a. Control Minocycline b. Control bTBc. bTBI Minocycline
a
b
c
Dorsal HippocampusGFAP/Cas3, 10x
a. Control Minocycline
b. Control bTBc. bTBI Minocycline
a
b
c
Dorsal HippocampusGFAP/Cas3, 60x
a. Control Minocycline
b. Control bTBc. bTBI Minocycline
a
b
c
Conclusions
Minocycline is effective in reducing inflammation and apoptotic cell death in the dorsal and ventral hippocampus following mild blast TBI.
The TeamKasper Lab
Dr. Andrei BlohkinMAJ Antoinnette ShinnCDR Stephanie BardackCol Susan PerryMohammad Elsayed
Dr. Neil Grunberg
VA Merit Award B5044R
Dr. Denes Agoston Dr. Erzsebet KovesdiAlaa KamnakshDr. Farid AhmedDaniel Wingo
Dr. Joseph Long, WRAIR