chris leigh-lancaster_inside 3d printing melbourne
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#3dprintconfmelbTRANSCRIPT
The World’s First Commercial 3D Bioprinter
Chris Leigh-Lancaster
Introducing Invetech
A synergistic mix of biomedical, automation, high
precision, fluid handling and user experience ...
All the right ingredients for bioprinting!
More than 30 years of product realization
Page 2
Diagnostics Cell Therapy Point of Care
Consumer
& Industrial
Introducing Invetech A diverse project mix
Page 3
Making Human Tissue The community need – Organ/tissue replacement
Page 4
Making Human Tissue The community need – Tissue for drug discovery
Page 5
Printing Human Tissue Science fiction becomes reality
Page 6
Making Human Tissue Step 1 – Cells are harvested aggregates and loaded into cartridges
Page 7
Page 8
Making Human Tissue Step 2 – The Bioprinter prints layers of Cell aggregates and BioGel
Extruded BioGel
lines
Cartridge with BioGel
or cell aggregates
3D print
nozzle
Page 9
Making Human Tissue Step 3 – Construct is placed into a bioreactor and incubated
The NovoGen MMX Bioprinter™ Key features
Page 10
• 3 high-precision motion
axes
• Dual print heads for
BioGel and cell layers
• Thermal control of the
BioGel
• Semi-automated loading
of print cartridges
• An intuitive GUI for rapid
vessel/tissue design
The NovoGen MMX Bioprinter™ Initial blood vessel printing on the Bioprinter
Page 11
500 μm glass
cartridge
dispense tip
BioGel structure
containing BioGel and
cell lines
Blood vessel
geometry within the
BioGel structure
Simple Operation Intuitive graphical user interface (GUI)
Page 12
WPF-based flexible GUI - customisable for future applications
Key focus is easy translation of simple tissue designs into real tissue
“mine sweeper” style designer for developing 2D geometric forms
Built-in scripting language for design of more complex 3D constructs
Simple Operation Cartridge loading & alignment
Page 13
Before the Bioprinter …
Blood vessel printing took 8 hours or more
With the Bioprinter – less than an hour
Cartridge loading & alignment took up to 45 mins
With the Bioprinter - less than 1 minute
Thanks to ...
Intuitive cartridge loading with precision
alignment
Automatic positive displacement dispense
Automatic laser calibration of cartridge tip
position
Precision Printing What’s needed for successful printing?
Page 14
Positional precision of better than ± 20 microns
Re-calibration of tip positions for each cartridge
Smooth dispense of cells and BioGel
Bio-compatible operation
100 µm human hair
Precision Printing How it’s done …
Page 15
Optical-grade robotic axes with single micron precision
Novel 3D laser tip calibration using custom interpolation algorithms
Closed loop micro-stepping motor control to minimize vibration
Designed for the bio-safety cabinet
From Science to Application A timeline from the first blood vessel
Page 16
December 2010
First human blood vessel
Time Magazine’s Best Inventions of 2010
Mar 2011
Partnerships with big pharma
Nov 2011
Australian Engineering Innovation Award.
Dec 2012
Autodesk partnership
Feb 2013
Refined Bioprinters delivered to Organovo
From Science to Application A timeline from the first blood vessel
Page 17
Apr 2013
First 3D liver tissue. Life span increases from 5 to 40 days
Apr 2014
Contracting for 3D liver tissue toxicity testing
The Near Future
3D tissue constructs replace existing pre-clinical trial methods for drug screening.
Simple tissues start being used for direct surgical therapy
Alternate Blood Vessel Manufacturing
Page 18
“Off the shelf” human veins for use in life-saving vascular surgery
Humacyte Artificial Vascular Graft (HAVGTM)
Regenerative Medicine SCORE center at Scripps Clinic- Cartilage printing
Page 19
Consumer Products Modern Meadow - Cow-less leather and meat
Page 20
Cell Therapies
Innovacell - ICES 13 and ICEF 15
Page 21
Argos Therapeutics - ArcelisTM
Treating incontinence and cancer
Page 22 Invetech confindential file: A3_3D Bioprinter - Inside 3D Printing Conference.pptx
www.invetech.us
www.linkedin.com/company/invetech
https://twitter.com/Invetech
Photo: Timothy Hogan