characterization of sugar-response arabidopsis (arabidopsis thaliana) mutants to engineer plants for...
TRANSCRIPT
Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy
protein productionBy Xin Li
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.
Purposes
• To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant.
• To produce cheaper soydiesel, more soy protein, and less expensive ethanol from the soybean plants.
Purposes
Modeling Soybean
Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
Modeling Soybean
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
Modeling Soybean
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Arabidopsis(Arabidopsis thaliana)
Soybean(Glycine max)
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
Modeling Soybean
•Both are oilseed plants
•Arabidopsis has a mapped genome
•Insertion-induced Arabidopsis mutants are av
•ailable commercially
•Insertion-induced Arabidopsis mutants are available commercially
Insertion-Induced Mutation
T-DNA
Gene
Insertion-Induced Mutation
Insertion-Induced Mutation
Insertion-Induced Mutation
Insertion-Induced Mutation
Insertion-Induced Mutation
Wild-type
Hypersensitive mutant
Insensitive mutant
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
• Measured root length of seedlings grown in glucose (Gibson, 2005)
Methods
• Grew 300 seedlings of each of 58 different mutants and wild-type in:
• 6% glucose
• 6% sucrose
• Measured root length of seedlings grown in glucose (Gibson, 2005)
• Use spectrophotometry to determine anthocyanin levels of seedlings grown in sucrose (Nacry,1998)
Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for
SALK_113292 are representative of all other 57 Arabidopsis mutants tested
0.000.200.400.600.801.001.201.401.601.802.00
SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072
Col-O
Root lengths
Results
Figure 1. Root lengths after 11 days: Results for the mutants shown in Figures 1 and 2 except for
SALK_113292 are representative of all other 57 Arabidopsis mutants tested
0.000.200.400.600.801.001.201.401.601.802.00
SALK_143055SALK_083114SALK_113292SALK_080380SALK_070394SALK_008072
Col-O
Root lengths
Results
Results
Results
ResultsNeff and Chory (1998)
Results
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is at1g06230.
Conclusion
• I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.
• The mutant’s genotype is SALK_113292.
• The disabled gene in mutant SALK_113292 is at1g06230.
• The gene contains a bromodomain protein
Future Work
• Grow SALK_113292 in 1% glucose and 1% sucrose
Future Work
• Grow SALK_113292 in 1% glucose and 1% sucrose
• Grow SALK_113292 in 1% sorbitol and 6% sorbitol
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Image from: htto://www.food-info.net
Acknowledgement
• Dr. Sue Gibson
• Dr. Chunyao Li
• Ms. Lois Fruen
• Ms. Chelen Johnson
• Dr. Jacob Miller
• Ms. Virginia Amundson
• Breck Advanced Team Research
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
http://www.cbs.umn.edu/plantbio/faculty/GibsonSue/
Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy
protein productionBy Xin Li
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.