8/14/2019 Characterization of a New Malignant Human T-Cell l...[Eur J Haematol. 1990] - PubMed Result

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Characterization of a new malignant human T-cell line (PFI-285) sensitive to ascorbic acid.

Helgestad J, Pettersen R, Storm-Mathisen I, Schjerven L, Ulrich K, Smeland EB, Egeland T,Srskaard D, Brgger A, Hovig T, et al.

Department of Pediatric Research, National Hospital of Norway, Oslo.

A new malignant human T-cell line-labelled PFI-285-has been isolated from a boy with malignant lymphoma.

Morphologically, the cells had characteristics of malignant lymphoid cells. The cells presented surface antigens

as early cortical lymphocytes and proliferated non-adherently as single cells, independent of T-cell growth factor

(IL-2), in liquid culture. The cells had undetectable levels of receptors for IL-2, were not clonogenic in soft agar,

but did form tumors in nude mice. Their establishment and continuous growth in vitro was dependent on the

number of cells inoculated and on the growth medium used. Cytogenetic alteration, HTLV-1 or reverse

transcriptase activity were not detected. The production of known T-cell derived lymphokines such as IL-2,

B-cell growth factor(s), alpha-interferon or granulocyte/macrophage colony stimulating or inhibiting factor(s)

was not detected. The cells had 5-8% natural killer (NK)-cell activity against NK-cell sensitive target cells

(K562) and were not sensitive for NK cells. A most unusual characteristic was the pronounced sensitivity of the

cells to ascorbic acid. Concentrations down to 50 mumol/l killed the cells within hours.

1: Eur J Haematol. 1990 Jan;44(1):9-17.

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Characterization of a new malignant human T-cell line (PFI-285) sensitive to

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Patient Drug Information

Source: AHFS Consumer Medication Information

Ascorbic Acid (Cecon Drops, Cenolate , Cevi-Bid , ...) Ascorbic acid is used

to prevent and treat scurvy, a disease caused by a lack of vitamin C in the body.