chapter 17. the central dogma transcription & translation three main steps for each: initiation...
TRANSCRIPT
Chapter 17
The Central Dogma
Transcription & TranslationThree main steps for each:
InitiationElongationTermination
Transcription: Initiation Promoters (TATAA) & transcription factors RNA Polymerase II & transcription initiation
complex DNA unwinds, transcription begins
Transcription: Elongation
RNA nucleotides added in 5’ to 3’ direction
Transcription: Termination
Prokaryotes: terminator signal
Transcription: Termination
Eukaryotes: polyadenylation signal sequence
RNA Processing in Eukaryotes5’ guanine cap & 3’ poly-A tail added
Facilitate export from nucleusPrevent degradation
RNA Processing in EukaryotesSpliceosome of
snRNP removes introns, leaves exons
RNA Processing in Eukaryotes
Translation – Basic InfoIn cytoplasmCodonsWobble
Translation – Basic InfoAnticodons & tRNA
Translation – Basic InfoAminoacyl tRNA synthetase
Translation – Basic InforRNA (ribosome) – two
subunits
Translation - InitiationInitiator tRNA hydrogen bonds at start codon (AUG)Requires GTPInitiation factors attach ribosomal subunits
Translation - ElongationA new tRNA with complementary anticodon
bonds at the A site.Requires GTP
Translation - ElongationPeptide bond forms between amino acids at P
& A sites
Translation - ElongationTranslocationRequires GTPEmpty tRNA is released
Translation - Elongation
Process repeats
Translation - TerminationRelease factor bonds at stop codon (UAG,
UAA, UGA)Hydrolyzes bond between peptide chain &
last tRNAAssembly separates
Translation
Polyribosomes
Free vs. Bound RibosomesAll start freeSignal peptide sequence on those bound for
ERSignal-recognition particle (SRP) attaches
Prokaryotes vs. EukaryotesDifferent RNA polymerasesDifferent transcription terminationDifferent sized ribosomal subunits
Prokaryotes vs. Eukaryotes
Coupled T & T in prokaryotes