centrifugation serum v4 - gbo€¦ · centrifugation time is a critical issue for reducing...
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page 1/15
Stability of VACUETTE® Serum Separator tubes with shortened centrifugation times
Background:
Greiner-Bio-One, Austria has been selling plastic evacuated tubes (VACUETTE®) for venous blood collection since 1986. Serum Tubes with and without gel separator are coated with micronised silica particles which activate clotting when the tubes are gently inverted. VACUETTE® Serum Separator Clot Activator tubes contain a barrier gel that is present in the bottom of the tube. The specific gravity of this material lies between the blood clot and the serum. During centrifugation, the gel moves upwards to the serum-cell interface where it forms a stable barrier separating the serum from the fibrin clot. This improves the serum yield and enables serum to be left in the primary tube for analysis. It further allows greater stability of certain analytes when kept under specified conditions. [1] Study Objective: Centrifugation time is a critical issue for reducing turn-around-time in laboratory workflow. A clinical study was carried out to evaluate two different centrifugation conditions in order to improve efficiency in laboratory processes. [2,3] Study design and procedure:
Venous blood was collected from 40 presumably healthy donors into four VACUETTE® Serum Separator tubes (456073, 13/100, 5 ml) using VACUETTE® Safety Blood Collection Sets with Holder (item # 450085). Two different VACUETTE® gel tubes (Sample A US P-Gel and Sample B European Gel) were included in this test. A discard tube was used to guarantee appropriate filling of the tubes. The order of collection was randomized. Directly after blood collection all the tubes were inverted 8 times for proper mixing. Tubes were allowed to clot for at least 30 min at room temperature in an upright position and then centrifuged with a Rotanta 460R centrifuge from Hettich at two different centrifugation conditions (see Table 1). Table 1: Centrifugation
Sample RCF (x g) Time Temp [°C]
Sample A P-Gel
1800g 10 min 20 °C
Sample A P-Gel
1800g 5 min 20 °C
Sample B Gel
1800g 10 min 20 °C
Sample B Gel
1800g 5 min 20 °C
All sample tubes were analyzed for the constituents listed below at the initial time point within 2 hours of blood collection and after 48 hours on the Olympus AU640 analyzer (chemistry assays) or the Abbott AxSYM® analyzer (immunoassay assays) using accompanying reagents from the instrument manufacturers. Between measurements, the samples were stored in the refrigerator at 4-8 °C. The following parameters were measured:
Alanine Aminotransferase (ALT) Albumin Alkaline Phosphatase (ALP) Aspartate Aminotransferase (AST) Calcium
Chloride Cholesterol Creatine Kinase (CK) Creatinine Gamma Glutamyltransferase (GGT)
page 2/15
Glucose Iron Lactate Dehydrogenase (LDH) Magnesium Phosphate Potassium Sodium Total Bilirubin
Total Protein Triglyceride Thyroid stimulating hormone (TSH) Urea Uric acid Free Triiodthyronin (fT3) Free Thyroxin (fT4 )
Results: Comparison analysis was performed at the initial time point and at 48 hours. Statistical evaluation was performed with the T-test (α = 0.05) using StatSoft Software, Version 9. One sample B centrifuged at 1800g for 10 minutes was found to be hemolyzed (donor 17) and was removed from the statistical data pool for AST, LDH and Potassium after outlier testing. Also, for some donors values for immunoassay parameters at 48 hours are missing due to insufficient sample volume. Clinical evaluation was based on the allowed recommendation by the German Medical Association (RILIBÄK). [4] No clinically significant differences were observed with either gel type for the two centrifugation settings of 1800g for 10 minutes and 1800g for 5 minutes at the initial time point or after 48 hours. Conclusion:
In this study, both gel types used in the VACUETTE® Serum Separator Clot Activator tubes showed comparable results for the two tested centrifugation conditions of 1800g for 10 minutes and 1800g for 5 minutes for the tested biochemical parameters at the initial time point and 48 hours. Differences in results were not clinically significant.
References:
[1] Instructions for Use. Evacuated Blood Collection System. For in vitro Diagnostic Use. Rev. 13 [2] O‘Keane M. P., Evaluation of three different specimen types (serum, plasma, lithium heparin and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use. Clin. Chem. Labor. Med. (44), 5 662-668 [3] Thomas L., Labor und Diagnose. Indikation und Bewertung von Laborbefunden für die medizinische Diagnostik. 7.Aufl. TH-Books Verlagsgesellsch. [4] Guideline from the medical association in Germany for quality assurance of laboratory tests. German Medical Journal. Vol. 105, Issue 7. 2008. [5] Olympus product insert. [6] AxSYM® product insert.
GBO03 page 3/15
Alanine Aminotransferase (ALT, GPT) Reference range male: <35 U/l Reference range female: < 31 U/l [5]
Sample A 0h Alanine Aminotrasferase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
[U/l]
A0 ALT 1800g_10min A0 ALT 1800g_5min
Sample B 0h
Alanine Aminotrasferase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
[U/l]
B0 ALT 1800g_10min B0 ALT 1800g_5min
Albumin Reference range: 35-53 g/l [5]
Sample A 0h Albumin 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
[g/l]
A0 ALB 1800g_10min A0 ALB 1800g_5min
Sample B 0h
Albumin 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
[g/l]
B0 ALB 1800g_10min B0 ALB 1800g_5min
Sample A 48h
Alanine Aminotransferase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
[U/l]
A48 ALT 1800g_10min A48 ALT 1800g_5min
Sample B 48h
Alanine Aminotransferase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
[U/l]
B48 ALT 1800g_10min B48 ALT 1800g_5min
Sample A 48h
Albumin 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
[g/l]
A48 ALB 1800g_10min A48 ALB 1800g_5min
Sample B 48h
Albumin 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
[g/l]
B48 ALB 1800g_10min B48 ALB 1800g_5min
GBO03 page 4/15
Alkaline Phosphatase: Reference range male: 40-130 U/l Reference range female: 55-105 U/l [5]
Sample A 0h Alkaline Phosphatase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[U/l]
A0 ALP 1800g_10min A0 ALP 1800g_5min
Sample B 0h
Alkaline Phosphatase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[U/l]
B0 ALP 1800g_10min B0 ALP 1800g_5min
Aspartate aminotransferase (AST, GOT) Reference range male: <45 U/l Reference range female: < 34 U/l [5]
Sample A 0h Aspartate Aminotransferase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[U/l]
A0 AST 1800g_10min A0 AST 1800g_5min
Sample B 0h
Aspartate Aminotransferase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[U/l]
B0 AST 1800g_10min B0 AST 1800g_5min
Sample A 48h
Alkaline Phosphatase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[U/l]
A48 ALP 1800g_10min A48 ALP 1800g_5min
Sample B 48h
Alkaline Phosphatase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[U/l]
B48 ALP 1800g_10min B48 ALP 1800g_5min
Sample A 48h
Aspartate Aminotransferase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[U/l]
A48 AST 1800g_10min A48 AST 1800g_5min
Sample B 48h
Aspartate Aminotransferase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[U/l]
B48 AST 1800g_10min B48 AST 1800g_5min
GBO03 page 5/15
Calcium (Arsenazo) Reference range: 2.20-2.65 mmol/l [5]
Sample A 0h Calcium Arsenazo 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
A0 CALA 1800g_10min A0 CALA 1800g_5min
Sample B 0h
Calcium Arsenazo 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
B0 CALA 1800g_10min B0 CALA 1800g_5min
Chloride Reference range: 95-105 mmol/l [5]
Sample A 0h Chloride 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mm
ol/l
]
A0 CL 1800g_10min A0 CL 1800g_5min
Sample B 0h
Chloride 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mm
ol/l
]
B0 CL 1800g_10min B0 CL 1800g_5min
Sample A 48h
Calcium Arsenazo 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
A48 CALA 1800g_10min A48 CALA 1800g_5min
Sample B 48h
Calcium Arsenazo 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
B48 CALA 1800g_10min B48 CALA 1800g_5min
Sample A 48h
Chloride 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mm
ol/l
]
A48 CL 1800g_10min A48 CL 1800g_5min
Sample B 48h
Chloride 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mm
ol/l
]
B48 CL 1800g_10min B48 CL 1800g_5min
GBO03 page 6/15
Cholesterol Reference range: <200 mg/dl [5]
Sample A 0h Cholesterol 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
50
100
150
200
250
300
350
[mg
/dl]
A0 CHOL 1800g_10min A0 CHOL 1800g_5min
Sample B 0h
Cholesterol 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
50
100
150
200
250
300
350
[mg
/dl]
B0 CHOL 1800g_10min B0 CHOL 1800g_5min
Creatine Kinase (CK) Reference range male: < 190 U/l Reference range female: <167 U/l [5]
Sample A 0h Creatine Kinase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
100
200
300
400
500
600
700
800
900
[U/l]
A0 CK 1800g_10min A0 CK 1800g_5min
Sample B 0h
Creatine Kinase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
100
200
300
400
500
600
700
800
900
[U/l]
B0 CK 1800g_10min B0 CK 1800g_5min
Sample A 48h
Cholesterol 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
50
100
150
200
250
300
350
[mg
/dl]
A48 CHOL 1800g_10min A48 CHOL 1800g_5min
Sample B 48h
Cholesterol 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
50
100
150
200
250
300
350
[mg
/dl]
B48 CHOL 1800g_10min B48 CHOL 1800g_5min
Sample A 48h
Creatinine Kinase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
100
200
300
400
500
600
700
800
900
[U/l]
A48 CK 1800g_10min A48 CK 1800g_5min
Sample B 48h
Creatinine Kinase 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
100
200
300
400
500
600
700
800
900
[U/l]
B48 CK 1800g_10min B48 CK 1800g_5min
GBO03 page 7/15
Creatinine Reference range male: 49-97 µmol/l Reference range female: 42-80 µmol/l [5]
Sample A 0h Creatinine 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[µmol/l]
A0 CREZ 1800g_10min A0 CREZ 1800g_5min
Sample B 0h
Creatinine 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[µmol/l]
B0 CREZ 1800g_10min B0 CREZ 1800g_5min
Gamma Glutamyltransferase (GGT) Reference range male: < 55 U/l Reference range female: <38 U/l [5]
Sample A 0h GGT 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
[U/l]
A0 GGT 1800g_10min A0 GGT 1800g_5min
Sample B 0h
GGT 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
50
[U/l]
B0 GGT 1800g_10min B0 GGT 1800g_5min
Sample A 48h
Creatinine 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[µmol/l]
A48 CREZ 1800g_10min A48 CREZ 1800g_5min
Sample B 48h
Creatinine 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[µmol/l]
B48 CREZ 1800g_10min B48 CREZ 1800g_5min
Sample A 48h
GGT 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
[U/l]
A48 GGT 1800g_10min A48 GGT 1800g_5min
Sample B 48h
GGT 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
50
[U/l]
B48 GGT 1800g_10min B48 GGT 1800g_5min
GBO03 page 8/15
Glucose Reference range: 74-109 mg/dl [5]
Sample A 0h Glucose 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mg
/dl]
A0 GLUC 1800g_10min A0 GLUC 1800g_5min
Sample B 0h
Glucose 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mg
/dl]
B0 GLUC 1800g_10min B0 GLUC 1800g_5min
Iron Reference range male: 6.3-30.7 µmol/l Reference range female: 4.1-29.5 µmol/l [5]
Sample A 0h Iron 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[µmol/l]
A0 IRON 1800g_10min A0 IRON 1800g_5min
Sample B 0h
Iron 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[µmol/l]
B0 IRON 1800g_10min B0 IRON 1800g_5min
Sample A 48h
Glucose 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mg
/dl]
A48 GLUC 1800g_10min A48 GLUC 1800g_5min
Sample B 48h
Glucose 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
[mg
/dl]
B48 GLUC 1800g_10min B48 GLUC 1800g_5min
Sample A 48h
Iron 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[µm
ol/l
]
A48 IRON 1800g_10min A48 IRON 1800g_5min
Sample B 48h
Iron 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
[µm
ol/l
]
B48 IRON 1800g_10min B48 IRON 1800g_5min
GBO03 page 9/15
Lactate Dehydrogenase (LDH) Reference range male: <248 U/l Reference range female:<247 U/l [5]
Sample A 0h Lactate Dehydrogenase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[U/l]
A0 LDH 1800g_10min A0 LDH 1800g_5min
Sample B 0h
Lactate Dehydrogenase 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[U/l]
B0 LDH 1800g_10min B0 LDH 1800g_5min
Magnesium Reference range: 0.75-1.10 mmol/l [5]
Sample A 0h Magnesium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
A0 MG 1800g_10min A0 MG 1800g_5min
Sample B 0h
Magnesium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
B0 MG 1800g_10min B0 MG 1800g_5min
Sample A 48h
LDH 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[U/l]
A48 LDH 1800g_10min A48 LDH 1800g_5min
Sample B 48h
LDH 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[U/l]
B48 LDH 1800g_10min B48 LDH 1800g_5min
Sample A 48h
Magnesium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
A48 MG 1800g_10min A48 MG 1800g_5min
Sample B 48h
Magnesium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
B48 MG 1800g_10min B48 MG 1800g_5min
GBO03 page 10/15
Phosphate, inorganic Reference range: 0.84-1.45 mmol/l [5]
Sample A 0h Inorganic Phosphate 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
A0 IP 1800g_10min A0 IP 1800g_5min
Sample B 0h
Inorganic Phosphate 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
B0 IP 1800g_10min B0 IP 1800g_5min
Potassium Reference range: 3.6-4.8 mmol/l [5]
Sample A 0h Potassium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
[mm
ol/l
]
A0 K 1800g_10min A0 K 1800g_5min
Sample B 0h
Potassium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
[mm
ol/l
]
B0 K 1800g_10min B0 K 1800g_5min
Sample A 48h
Inorganic Phosphate 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
A48 IP 1800g_10min A48 IP 1800g_5min
Sample B 48h
Inorganic Phosphate 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
B48 IP 1800g_10min B48 IP 1800g_5min
Sample A 48h
Potassium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
[mm
ol/l
]
A48 K 1800g_10min A48 K 1800g_5min
Sample B 48h
Potassium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
[mm
ol/l
]
B48 K 1800g_10min B48 K 1800g_5min
GBO03 page 11/15
Sodium Reference range: 135-145 mmol/l [5]
Sample A 0h Sodium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[mm
ol/l
]
A0 Na 1800g_10min A0 Na 1800g_5min
Sample B 0h
Sodium 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[mm
ol/l
]
B0 Na 1800g_10min B0 Na 1800g_5min
Total Bilirubin Reference range: 0.1-1.2 mg/dl [5]
Sample A 0h Total Bilirubin 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mg
/dl]
A0 TBIL 1800g_10min A0 TBIL 1800g_5min
Sample B 0h
Total Bi lirubin 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
[mg
/dl]
B0 TBIL 1800g_10min B0 TBIL 1800g_5min
Sample A 48h
Sodium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[mm
ol/l
]
A48 Na 1800g_10min A48 Na 1800g_5min
Sample B 48h
Sodium 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
[mm
ol/l
]
B48 Na 1800g_10min B48 Na 1800g_5min
Sample A 48h
Total Bil irubin 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
2,2
2,4
2,6
2,8
3,0
[mg
/dl]
A48 TBIL 1800g_10min A48 TBIL 1800g_5min
Sample B 48h
Total Bil irubin 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
2,2
2,4
2,6
2,8
3,0
[mg
/dl]
B48 TBIL 1800g_10min B48 TBIL 1800g_5min
GBO03 page 12/15
Total Protein Reference range: 66-83 g/l [5]
Sample A 0h Total Protein 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
80
90
[g/l]
A0 TP 1800g_10min A0 TP 1800g_5min
Sample B 0h
Total Protein 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
80
90
[g/l]
B0 TP 1800g_10min B0 TP 1800g_5min
Triglyceride Reference range: <150 mg/dl [5]
Sample A 0h Triglyceride 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[mg
/dl]
A0 TRI 1800g_10min A0 TRI 1800g_5min
Sample B 0h
Triglyceride 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[mg
/dl]
B0 TRI 1800g_10min B0 TRI 1800g_5min
Sample A 48h
Total Protein 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
80
90
[g/l]
A48 TP 1800g_10min A48 TP 1800g_5min
Sample B 48h
Total Protein 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
60
70
80
90
[g/l]
B48 TP 1800g_10min B48 TP 1800g_5min
Sample A 48h
Triglyceride 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[mg
/dl]
A48 TRI 1800g_10min A48 TRI 1800g_5min
Sample B 48h
Triglyceride 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
20
40
60
80
100
120
140
160
180
200
220
240
260
[mg
/dl]
B48 TRI 1800g_10min B48 TRI 1800g_5min
GBO03 page 13/15
Thyroid stimulating hormone (TSH) Reference range: 0.49-4.67 µU/ml [6]
Sample A 0h TSH 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[µU/m
l]
A0 TSH 1800g_10min A0 TSH 1800g_5min
Sample B 0h
TSH 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[µU/m
l]
B0 TSH 1800g_10min B0 TSH 1800g_5min
Urea Reference range male: 18-55 mg/dl Reference range female: 15-43 mg/dl [5]
Sample A 0h Urea 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
[mg
/dl]
A0 UREA 1800g_10min A0 UREA 1800g_5min
Sample B 0h
Urea 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
10
20
30
40
50
[mg
/dl]
B0 UREA 1800g_10min B0 UREA 1800g_5min
Sample A 48h
TSH 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[µU/m
l]
A48 TSH 1800g_10min A48 TSH 1800g_5min
Sample B 48h
TSH 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[µU/m
l]
B48 TSH 1800g_10min B48 TSH 1800g_5min
Sample A 48h
Urea 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
50
[mg
/dl]
A48 UREA 1800g_10min A48 UREA 1800g_5min
Sample B 48h
Urea 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
5
10
15
20
25
30
35
40
45
50
[mg
/dl]
B48 UREA 1800g_10min B48 UREA 1800g_5min
GBO03 page 14/15
Uric acid Reference range male: 3.6-7.0 mg/dl Reference range female: 2.3-6.1 mg/dl [5]
Sample A 0h Uric Acid 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
7
8
9
10
[mg
/dl]
A0 UA 1800g_10min A0 UA 1800g_5min
Sample B 0h
Uric Acid 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
7
8
9
10
[mg
/dl]
B0 UA 1800g_10min B0 UA 1800g_5min
Free Triiodthyronin (fT3)
Reference range: 1.45-3.48 pg/ml [6]
Sample A 0h fT3 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[pg
/ml]
A0 fT3 1800g_10min A0 fT3 1800g_5min
Sample B 0h
fT3 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[pg
/ml]
B0 fT3 1800g_10min B0 fT3 1800g_5min
Sample A 48h
Uric Acid 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
7
8
9
10
[mg
/dl]
A48 UA 1800g_10min A48 UA 1800g_5min
Sample B 48h
Uric Acid 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420
1
2
3
4
5
6
7
8
9
10
[mg
/dl]
B48 UA 1800g_10min B48 UA 1800g_5min
Sample A 48h
fT3 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[pg
/ml]
A48 fT3 1800g_10min A48 fT3 1800g_5min
Sample B 48h
fT3 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
[pg
/ml]
B48 fT3 1800g_10min B48 fT3 1800g_5min
GBO03 page 15/15
Free Thyroxin (fT4)
Reference range: 0.71-1.85 ng/dl [6]
Sample A 0h fT4 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
A0 fT4 1800g_10min A0 fT4 1800g_5min
Sample B 0h
fT4 0h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
B0 fT4 1800g_10min B0 fT4 1800g_5min
Sample A 48h
fT4 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
A48 fT4 1800g_10min A48 fT4 1800g_5min
Sample B 48h
fT4 48h
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 420,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
B48 fT4 1800g_10min B48 fT4 1800g_5min