center for biotechnology biosciences institute dept · topics of research: regulation of the...

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Ongoing research: Production of bioactive monoterpene indole alkaloids in Psychotria spp. Production of immunoadjuvant saponins in Quillaja brasiliensis. Production of terpenes (oleoresin) in Pinus elliottii plantations. Search for new bioactivities and in planta functions of secondary metabolites. Control of adventitious rooting in Eucalyptus (emphasis on E. globulus and its hybrids), native woody species and Arabidopsis thaliana. Approaches: Physiological (whole plant, tissues, cells) , biochemical (enzyme activities and metabolite quantification), and molecular (gene expression). TOPICS OF RESEARCH: Regulation of the production of bioactive secondary metabolites in plants. Control of adventitious root development in forest and model species. BIOLOGICAL SCIENCES PLANT PHYSIOLOGY BOTANY SECONDARY METABOLISM AND DEVELOPMENT N NH H H O COOCH 3 O G lc H H OH H H A B C CONTACT: Arthur Germano FettNeto, Ph.D. 55 51 3308 7642 [email protected] www.cbiot.ufrgs.br/cenbiot/fisiovegetal/index.html CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE Figura 1. Representative images of ongoing investigations: A. Psychotria brachyceras and its antioxidant and antimutagenic alkaloid, brachycerine; B. Oleoresin tapping (collected in plastic bags) mediated by induction paste (applied to wound panel) in Pinus elliottii; C. Adventitious rooting in microcuttings of Eucalyptus globulus induced by auxin. CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE DEPT OF BOTANY

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Page 1: CENTER FOR BIOTECHNOLOGY BIOSCIENCES INSTITUTE DEPT · TOPICS OF RESEARCH: Regulation of the production of bioactive secondary metabolites in plants. Control of adventitious root

Ongoing research:‐Production of bioactive monoterpene indole alkaloids in Psychotria spp.‐ Production of immunoadjuvant saponins in Quillaja brasiliensis.‐ Production of terpenes (oleoresin) in Pinus elliottii plantations.‐ Search for new bioactivities and in planta functions of secondary metabolites.‐ Control of adventitious rooting in Eucalyptus(emphasis on E. globulus and its hybrids), native woody species and Arabidopsis thaliana.  

Approaches:  Physiological (whole plant, tissues, cells) , biochemical (enzyme activities and metabolite quantification), and molecular (gene expression).

TOPICS OF RESEARCH: Regulation of the production of bioactive secondary metabolites in plants. Control of adventitious root development in forest and model species.

BIOLO

GICAL SCIENCE

SPLANT PH

YSIOLO

GY

BOTA

NY

SECO

NDARY

 METABO

LISM

 AND 

DEV

ELOPM

ENT

NN H

HH

O

CO O CH 3

OGlc H

H

O HH

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A

B

C

CONTACT: Arthur Germano Fett‐Neto, Ph.D.55 51 3308 [email protected]/cenbiot/fisiovegetal/index.html

CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTECENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE

Figura  1. Representative  images  of  ongoing investigations: A. Psychotria brachyceras and  its antioxidant  and  antimutagenic  alkaloid, brachycerine;  B. Oleoresin  tapping  (collected  in plastic  bags)  mediated  by  induction  paste (applied  to  wound  panel)  in  Pinus  elliottii;  C. Adventitious  rooting  in  microcuttings  of Eucalyptus globulus induced by auxin. 

CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE DEPT OF BOTANY

Page 2: CENTER FOR BIOTECHNOLOGY BIOSCIENCES INSTITUTE DEPT · TOPICS OF RESEARCH: Regulation of the production of bioactive secondary metabolites in plants. Control of adventitious root

Approaches: Physiological problems are investigated  using molecular techniques (differential and quantitative gene expression), biochemical (enzyme activities, primary and secondary metabolites) and of whole plant (growth and morphometric analyses). Studies are carried out both in the laboratory and in the field.

Main results (examples): Increased yield of bioactive products originated from Brazilian phytodiversity (Figure 2); Establishment of propagation protocols for native species that accumulate bioactive molecules aiming at sustainable production systems (Figures 3 and 4); Development of oleoresin induction pastes with low cost and resulting in high yield; Development of optimized rooting protocols for Eucalyptus (Figure 5).   

MAIN RESULTS: efficient strategies for production of alkaloids and terpenes with pharmaceutical and industrial interest. Development and optimization of adventitious rooting systems for forestry and bioactive woody species .

FORE

STRY

 AND 

AGRICU

LTURE

USE OF BIODIVER

SITY

 AND PLA

NT BIOMASS

PLANT PR

ODUCT

ION

BIOACT

IVE PR

ODUCT

S AND PRO

CESS 

OPT

IMAIZATION

DEPT OF BOTANYCONTACT: Arthur Germano Fett‐Neto, Ph.D.55 51 3308 [email protected]/cenbiot/fisiovegetal/index.html

CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE

Figure  2.  Induction of  leaf accumulation of  the antioxidant  and  antimutagenic  alkaloidbrachycerine in  Psychotria brachyceras by UV exposure.

Figure  3.  Rooted cutting of  Psychotriaumbellata,  structure of  the neuroactivealkaloid psychollatine and a detail of  somaticembryo obtained in culture. 

Figure  4.  Microcuttings of  Quillaja brasiliensis, tree species that produces immunoadjuvantsaponins,  after exposure to  differentrhizogenesis promoting treatments.

Figure  5.  Microcuttings of  Eucalyptusglobulus rooted with different calciumconcentrations.

PLANT PHYSIOLOGYPLANT PHYSIOLOGY