center for biotechnology biosciences institute dept · topics of research: regulation of the...
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Ongoing research:‐Production of bioactive monoterpene indole alkaloids in Psychotria spp.‐ Production of immunoadjuvant saponins in Quillaja brasiliensis.‐ Production of terpenes (oleoresin) in Pinus elliottii plantations.‐ Search for new bioactivities and in planta functions of secondary metabolites.‐ Control of adventitious rooting in Eucalyptus(emphasis on E. globulus and its hybrids), native woody species and Arabidopsis thaliana.
Approaches: Physiological (whole plant, tissues, cells) , biochemical (enzyme activities and metabolite quantification), and molecular (gene expression).
TOPICS OF RESEARCH: Regulation of the production of bioactive secondary metabolites in plants. Control of adventitious root development in forest and model species.
BIOLO
GICAL SCIENCE
SPLANT PH
YSIOLO
GY
BOTA
NY
SECO
NDARY
METABO
LISM
AND
DEV
ELOPM
ENT
NN H
HH
O
CO O CH 3
OGlc H
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CONTACT: Arthur Germano Fett‐Neto, Ph.D.55 51 3308 [email protected]/cenbiot/fisiovegetal/index.html
CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTECENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE
Figura 1. Representative images of ongoing investigations: A. Psychotria brachyceras and its antioxidant and antimutagenic alkaloid, brachycerine; B. Oleoresin tapping (collected in plastic bags) mediated by induction paste (applied to wound panel) in Pinus elliottii; C. Adventitious rooting in microcuttings of Eucalyptus globulus induced by auxin.
CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE DEPT OF BOTANY
Approaches: Physiological problems are investigated using molecular techniques (differential and quantitative gene expression), biochemical (enzyme activities, primary and secondary metabolites) and of whole plant (growth and morphometric analyses). Studies are carried out both in the laboratory and in the field.
Main results (examples): Increased yield of bioactive products originated from Brazilian phytodiversity (Figure 2); Establishment of propagation protocols for native species that accumulate bioactive molecules aiming at sustainable production systems (Figures 3 and 4); Development of oleoresin induction pastes with low cost and resulting in high yield; Development of optimized rooting protocols for Eucalyptus (Figure 5).
MAIN RESULTS: efficient strategies for production of alkaloids and terpenes with pharmaceutical and industrial interest. Development and optimization of adventitious rooting systems for forestry and bioactive woody species .
FORE
STRY
AND
AGRICU
LTURE
USE OF BIODIVER
SITY
AND PLA
NT BIOMASS
PLANT PR
ODUCT
ION
BIOACT
IVE PR
ODUCT
S AND PRO
CESS
OPT
IMAIZATION
DEPT OF BOTANYCONTACT: Arthur Germano Fett‐Neto, Ph.D.55 51 3308 [email protected]/cenbiot/fisiovegetal/index.html
CENTER FOR BIOTECHNOLOGY & BIOSCIENCES INSTITUTE
Figure 2. Induction of leaf accumulation of the antioxidant and antimutagenic alkaloidbrachycerine in Psychotria brachyceras by UV exposure.
Figure 3. Rooted cutting of Psychotriaumbellata, structure of the neuroactivealkaloid psychollatine and a detail of somaticembryo obtained in culture.
Figure 4. Microcuttings of Quillaja brasiliensis, tree species that produces immunoadjuvantsaponins, after exposure to differentrhizogenesis promoting treatments.
Figure 5. Microcuttings of Eucalyptusglobulus rooted with different calciumconcentrations.
PLANT PHYSIOLOGYPLANT PHYSIOLOGY