cellometer vision cba analysis system & denovo fcs express
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Cellometer® Vision CBA Analysis System & DeNovo FCS Express 4 Softwarefor Image-Based Analysis of Cell-Based Assays
www.nexcelom.com+1 [email protected]
Vision CBA Analysis System Features•Cell-based assays with 20µl of sample
•No washing. No clogging. Simple Set-up.
•Cellimagesfordataverification
•Pattern-recognition software for analysis of cell clumps and irregular-shaped cells
•Results in 60 seconds
•User-changeable fluorescence optics modules ranging from UV to near IR/far red
•Experienced applications specialists and technical support
FCS Express 4 Software Features•Colorized scatter plots and histograms
•More accurate gating for enhanced sensitivity
•Pre-set, optimized data layouts
•Simple import and creation of data layouts for new assays
•Flow-like data presentation
•Multi-sample data formatting
•Convenient Export to Excel
Flow core labs can utilize the Vision CBA instrument to pre-screen samples prior to sorting and flowcytometryanalysisandtorun1-colorand2-colorassaysforcellcycle,apoptosis,GFPtransfection,mitochondrialpotential,andmore…freeinguptheirflowsystemforthemorecomplex studies.
Individual researchers performing simple 1-color and 2-color analysis appreciate the simplicity and accessibility of the Vision CBA. The ability to analyze samples right away, using only 20µl of sample, enables researchers to accelerate and expand their experiments. Researchers can analyze a number of time-points, rather than one endpoint result. The FCS Express 4 Softwareoffersstraightforwarddataanalysisproceduresandoutputsfamiliartomanyflowusers.
www.denovosoftware.com+1 [email protected]
Get Flow Results without Flow CytometryIncorporation of FCS Express 4 Software into the Vision CBA Analysis System has enabled users to conduct image-based analysis of cell-based assays requiring enhanced gating for the most accurate, reliable, and reproducible results. Side-by-side comparison of results for apoptosis, cell cycle, mitochondrial potential, autophagy, ABC transporter assays, aggresome detection, CD4+ / CD8+ immunophenotyping, and other assays has shown Cellometer image-based analysis results to be comparabletoflowcytometry.
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Cell Images
Livecellimages,bothbrightfieldandfluorescent,arecapturedforimmediatereview, exporting, and archiving. The counted brightfieldimageindicatesindividualcellscounted and can be used to optimize cell size parameters for the most accurate analysis. Visuallyconfirmthatcellswithinclumpsarebeing counted correctly and debris has been eliminated from the results.
www.nexcelom.com | 978.327.5340 | [email protected] Rev.A 01/12
Additional FCS Express 4 data layouts for the growing menu of Cell-Based Assays for the Cellometer Vision CBA Analysis System are constantly being created. Please contact Nexcelom technical support at 978-327-5340 or [email protected] for more information.
Looking for Data Layouts for other Cell-Based Assays?é
Customized Data Layouts
As shown in the Nexcelom data layout to the left, FCS Express 4 Softwareplotsdatabasedonfluorescenceintensityanddefaultgating parameters. The data table displays the cell percentage andcellconcentrationforeachphaseofthecellcycle.Gatingismanually optimized by dragging the gating bars in the histogram. Cell percentages and concentrations are instantly updated in the associated data table.
For analysis of Apoptosis with Annexin V-FITC / PI, FCS Express 4 Software generates colorized scatter plots indicating live, apoptotic and dead cells with an associated data table indicating the % of cells and cell concentration in each category.