cellmissy: enabling management and dissemination of cell migration data
TRANSCRIPT
CellMissy: enabling management and dissemination of cell migration data
paola masuzzo
[email protected] / Ghent University, Ghent, Belgium
A big challenge in cell migrationis data management and dissemination
sample preparation
automated image acquisition
automated image analysis
A big challenge in cell migrationis data management and dissemination
sample preparation
automated image acquisition
automated image analysis
There are three key elements to software for cell migration data
Guide and capture experimental set-up
Store, structure and query the acquired data
(Semi-)automate data exploration, analysis and interpretation
That’s why we developed CellMissy…
CellMissy modules
Experiment
0 3h 6hwound
cells
Experiment Manager
DataLoader
Data Analyzer
Masuzzo et al., Bioinformatics, 2013
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
Detailed metadata annotationis essential for data interpretation
Detailed metadata annotationis essential for data interpretation
Detailed metadata annotationis essential for data interpretation
Image acquisition metadata also tellan important part of the story
Link to raw images (ordering of imaged wells)
Imaging techniques
Exposure time, light intensity, magnification, time interval
Metadata are crucial
Exchanging the datalab-to-labglobal
Local data management
Structuring the data takes a bit of effort
These data support analysis on multiple levels
These data support analysis on multiple levels
These data support analysis on multiple levels
These data support analysis on multiple levels
These data support analysis on multiple levels
These data support analysis on multiple levels
Metadata and data are capturedon all these different levels
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
Metadata and/or data can be easily exchanged between laboratories
lab A
Metadata and/or data can be easily exchanged between laboratories
lab A lab B
Metadata and/or data can be easily exchanged between laboratories
lab A lab B
This is one file in CellMissy! (≈10 MB)
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
The real target is a global repositoryfor cell migration data
DataRepository
The real target is a global repositoryfor cell migration data
DataRepository Local Software
http://cellmissy.googlecode.com
Thank you
Niels Hulstaert prof. dr. Lennart
Martens
prof. dr. Leen Van Troysprof. dr. Christophe
Ampe