cell::explorer hts pro - perkinelmer:explorer hts pro was designed to fulfill those diverse needs....
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S P E C I F I C A T I O N S
Integrated Solutionscell::explorer HTS pro
Application ConfigurationMultimodule Ultra-High Throughput Screening (uHTS) Platform
Customer Request
A multimodule system that can accommodate both uHTS and HCS in one, integrated platform.
Configuration Requirements
• Fully integrated screening platform to support:
– 96, 384, 384 low volume and 1536-well plates
– nLtohighμLliquidhandling
– Detection instruments for HCS – image based readouts
– Cellular and biochemical multimode read-outs
• Parallel assay processing and offline use of individual instruments
• Parallel and offline access to both workstation modules
Today’sacademicresearchersrequiretoolsandtechnologiesthat can support a broad range of current and future applicationsincludingdifferentmicroplateformats,liquidhandling volumes and detection-based read-out methods; cell::explorer HTS pro was designed to fulfill those diverse needs. The configuration was created with four (4) individual workstations, each able to run independent processes, linked via a bi-directional con veyor belt to create a large integrated screening system poised to automate the entire screening process. Through two (2) integrated 500-plate capacity stores, the compound module has access to nearly 600,000 compounds stored in 384-well plates. Compounds can be transferred into assay plate either in nL amounts utilizingaLabcyteECHOacousticdispenserorinμLamounts through a JANUS® Automated Workstation fitted with both 96- and 384-well pipetting heads. Precision dispensing of reagent and cells is realized through a Flexdrop® IV and two Thermo MultiDrop® Combi dispenser spread over the indi vidual modules. A 198-plate incubator, as well as 40-plate incubator enable plate incubation at different environmental condi tions. Assay results are detected either on an EnVision® Multilabel Plate Reader or on an Opera® confocal microplate imaging reader.
3D view of multimodule uHTS platform
FlexDrop® IV High precision reagent & cell dispenser
Conveyor beltPlate distribution conveyor dispenser
Assay plates Compound platesExample Assay Flow
Image plate
Compound store
Compound store
Incubator 1
Add reagent 1
Add reagent 2Read barcode
Incubator
Incubator
JANUS compoundaddition
Wash plate
Wash
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PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com
Top view of multimodule HTS and HCS platform
Cytomat® 6001 IncubatorIncubation
Echo®
nl compound transfer
Washer
MultiDrop® CombiReagent dispenser
500 DC storeCompound plate storage
1000 DC storeCompound plate storage
MultiDrop® CombiReagent dispenser
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PerkinElmer Components
3rd Party Components
EnVision®
Multilabel Plate Reader
plate::handler™ 6-axis robotPlate handling robot
plate::handler™ 6-axis robotPlate handling robot
TurntablePlate handling device
Opera®
Confocal microplate imaging reader
JANUS® Automated WorkstationMINI Varispan®
with 4 tips
plate::handler™ 6-axis robotPlate handling robot
Lid handling unitLid removal, storage
and replacement
JANUS® Automated WorkstationMINI MDT for 96/384 tips
VSpin centrifugePlate centrifugation
View of system within a research facility