c:\documents and settings\user\desktop\microsoft power point agranulocytic series+abnormal wbc
TRANSCRIPT
Monoblast
Monocyte
Promonocyte
MonoblastSize:- ��� ���������� 10-18 �����
Cytoplasm:-)*� +,-��*-�.-����������-�����+�/
Nucleus:- �3456��+5�7��3����� ���(folded over)
-chromatin� ���@��A��+����+��)B3���C�
-)*� +��3�D� -E/ nucleoli ��456� 1-2 �B
PromonocyteSize:- ��� ���������� 14-18 �����
Cytoplasm:-)*� +,-��*�.,�����+�/ -E/ fine azurophilic granules
Nucleus:-�B�JKA�+5�7��3�-chromatin 5�/�L, ��+��)B3�������C� -)*� +��3�D�-E/ parachromatin-E/ nucleoli ���/��
Monocyte
Size:- ��� ���������� 14-18 �����
Cytoplasm:-)*� +MN-�.,���������+�/ -�DE/ fine azurophilic granule- �DE/ vacuoles
Nucleus:-�B�JKA�+5�7��3�-chromatin 5�/�L, C)��B�� ���@���+��)B3�������C�
-)*� +��3�D�-E/ parachromatin
Macrophage
Size:- ��� ���������� 25-50 �����
Cytoplasm:-�+P�*�K�� - �+ vacuoles- �+ azurophilic granule
Nucleus:-���-chromatin 5�/C�A�PQD,--�+ nucleoli 1-2 �B
Histiocyte
Histiocyte
��� Reticuloendothelial cell (RE cell) ��������� �� �!" # $ % &! " �� �! " "!� � ' ���%� "()*+,)+-(./
e
LymphoblastSize:- ��� ���������� 10-18 �����
Cytoplasm: -�+P�*�K��� -����+ granules-�DE/ perinuclear zone
Nucleus:- �3456������456�- �B)� �3��� Nucleus/Cytoplasm = 5-7/1-chromatin �A��+����+��C�-E/ nucleoli 1-2 �B
Prolymphocyte
Size:- ��� ���������� 10-18 �����
Cytoplasm:-)*� +,-��*4 -E/ azurophilic granules
Nucleus:-�B�JKA���,����@���-chromatin 5�/�L, ��+��)B3�PQ�A�/+�/C�
-�DE/ nucleoli ���
Lymphocyte
Size:- ��� ���������� 10-15 �����()�U*���� Lymphocyte)
Cytoplasm:-)*� +,-��*4 -�DE/ azurophilic granule
Nucleus:-�����@��� ,�+ 5�7� ��P�)-chromatin 5�/ ��+��)B3C���+�/
Large lymphocyte
Medium lymphocyte
Small lymphocyte
Large Granular Lymphocyte “N.K cell”
Spindle-shaped lymphocyte
Atypical lymphocyte
(Transformed lymphocyte)
�PQ lymphocyte .+W����4�A�A.+W�+���P�+W�CP����P��� �7W��D�����4�A�A active stage ��� 3 C// �7�
Type IType 2Type 3
Atypical lymphocyte type I
Size:-�PQ cell .+W�+�����C�� P�)*��������456�
Cytoplasm:-)*� +,-��*��������plasma cell �PQ foamy -�DE/ fine azurophilic granules
Nucleus:-���5�7���P�)-chromatin 5�/��+��)B3����PQ�A�/+�/
Size:-��456��3� cell type I
Cytoplasm: -)*� +,-��*4 -�D)*� +����)���/
Nucleus:-��� , ��P�) 5�7�����nucleus ��� monocyte-chromatin 5�/��+��)B3C�
Atypical lymphocyte type II
Size:-P�)*��@��3� cell type I
Cytoplasm: -)*� +,-��*-�D)*� +����)���/
Nucleus:-�B���� ,�+ 5�7� ��P�)-chromatin �A��+����+��)B3�PQ�A�/+�/- ��[E/ nucleoli
Atypical lymphocyte type III
Plasmoblast
Proplasmocyte
Plasma cell
Plasmoblast
Size:- ��� ���������� 18-25 �����
Cytoplasm: - +,-��*����-�B�JKA foamy-�+ perinuclear zone
Nucleus:-��456�,�B�U*���/ -E/ nucleoli 5���B-�DE/����+��)B3��� parachromatin �PQ��P�����3+� (Cartwheel)
Proplasmocyte
Size:- ��� ���������� 15-25 �����
Cytoplasm: - +,-��*����-�B�JKA foamy -�+ perinuclear zone UB��D
Nucleus:-����@���-�B�U*���/ -�DE/ nucleoli 5���5�7�����
Plasma cell
Size:- ��� ���������� 8-24 �����
Cytoplasm: - +,-��*����-�B�JKA foamy UB��D-�+ perinuclear zone ���UB�
Nucleus:-����@���-�B�U*���/ -�DE/ 2 nucleus -�+ parachromatin UB��D
������������� ���������������������������������������� �!�"#�����$%���#���&%���$% '(��#���&%���$%�!��)��%�)��:
•Congenital abnormalities•Acquired abnormalities
������������ ��&%���$% ������+ 2222 ��� ,���-.��1.1.1.1. CytoplasmicCytoplasmicCytoplasmicCytoplasmic abnormalities abnormalities abnormalities abnormalities
• .���&%���$% �������������� granulegranulegranulegranule
• �����%�/�������" cytoplasmcytoplasmcytoplasmcytoplasm ((((vacuolization) vacuolization) vacuolization) vacuolization)
2.2.2.2. Nuclear abnormalitiesNuclear abnormalitiesNuclear abnormalitiesNuclear abnormalities
0 �PQ Large azurophilic granules ��456� 5�/ C�A)*� +����
0 E/���4]3A:- Infections- Serious burns- Septicemia- drug poisoning
Cytoplasmic abnormalities
.���&%���$% �������������� granule
Normal1.Toxic granule
Toxic granule
0 Congenital (autosomal recessive)0 �PQ granule �+��456�)*� +��3�C��(Abnormal azurophilic granules) E/���4��@���7���3.̀�U*�0 Susceptible to infection�+�B�JKA��3��B/albinismC�A bleeding tendency
2.Chediak- Higashi anomaly
0 )A������@���@���� 5�7���P��� �� 1-4 µm0 )*� +,-��* �B�DA����U*���/�b��� bLW�E/���4 bandform, neutrophil0 Aggregates of Rough endoplasmic reticulum0E/4]3A:
- Several toxic status- Severe burn- Burns- Chemotherapy- Malignant disease- Pregnancy
0 DA5��P��7W���B/��� ��]3AP�)*
3. Döhle bodies
4. Alder-Reilly anomaly
0 Autosomal recessive trait0 E/ granule ��@���456� )*� +��3�������AD�.B / nucleus � � � neutrophil C � A � DE/ 4 lymphocyte 5�7� monocyte0 ���� Toxic granules
0 C���3�C)�)��.+W Cytochemical stains:h����� Alder-Reilly45���/3�4��������3� Toluidine blue stain C�A Colloidal iron stain �3 Toxic granule 45��� i�/j.B,����
5. Auer rod
0 �PQC.�� + ��3�-C�� 0 ��*�D� Abnormal development of
primary granules0 E/4]3A Leukemic Blood cells ���
- Myeloblast- Promyelocyte- Monoblast
6. May-Hegglin anomaly
0 Autosomal dominant trait 0 �B�JKA�PQ����� 1-3 m, + blue-gray ���� Döhle bodies C)�DAE/)����P 0 ��*�D� Aggregates of mRNA0 �B�E/��3��B/
LeukopeniaThrombocytopeniaE/ Giant platelets
0 4]3A.+W�+��)*��U7,������̀C��
1 ��E/ vacuole ��3��B/���+ toxic granule 4 neutrophil DAU�3�/��U+,���+]3A septicemia
0 ����@/)B3������7���3�4 ��B��7��C�@�C�A)B,��3�.+W�̀K5]��*5��� ��* 4 UBW3h��
Cytoplasmic abnormalities
/�������" cytoplasm (vacuolization)
Nuclear abnormalities
1. Hypersegmentation
0 Nucleus C/���PQ lobe ���3� 5 lobes �L,�P 0 ihypersegmented neutrophilj0 Vit.B12 or Folic acid Deficiency(Megaloblastic anemia or Pernicious anemia)
0 h���)*4 nucleus �����@���7���3DB/��̀���BC� .-45�E/ neutrophil �+ 2 lobe ����C3�) �B�JKA���� band form 0 Congenital (Autosomal dominant) 0 Acquire
h����7��.+W�+���D�*6�������A������*�P�)* (myeloproliferative disorder)�+��)*��U7,��̀C��
2. Pelger-Huet anomaly
3. Pyknotic cell
0Nucleus �+����@��� �3��B�PQ�����+�3 C� C�A�PQ�B33 0Cytoplasm �B��� ]E45��5@���� 0E/4�b���.+W�-�B�DA ��)B3
0 �PQ U*, �3��� cells .+W)�C��3 nucleus [��.-�� DL��5@�PQ���C5 0 ��[E/���P�)*C�AE/������3�P�)*4 Leukemic blood cell h���sEA4 Chronic Lymphocytic Leukemia (CLL)
4. Smudge cell or basket cell
0 �PQ WBC h���sEA PMN .+W�* hematoxylin body0 ��*�D�:Autoimmune disease h�����Pu3��+ Antinuclearfactor (ANF)0�PQPv*��*� Antigen-antibodies complex ��+�� complex+,3� Hematoxylin body (Nucleus:ANF)0 �+ Complement���A��A)̀�45���*��/3�� Chemotaxis
0 PMN ���A�3��B��+�� Rosettes form0 PMN �E+��)B3��+�3.+W�* Hematoxylin body ����P��+��3� iL.E cellj
Rosettes form
L.E.Cell
1. L.E cell
Other
0 �7� monocyte 5�7� neutrophil .+W��*� phagocytosis0 �B����5@�B�JKAh���)*��� nucleus ����b���.+W[���*����PUB��D
2. Tart cell