ccb-tox continuous contamination biomonitor benefits, functions & applications confidential...

CCB-TOX

Continuous Contamination Biomonitor

Benefits, Functions & Applications

Confidential & Preliminary

Contents

• Introduction

•Function

•Application

•Advantages

CCB-TOX

Introduction: what is the CCB-TOX?

• Automatic water toxicity biomonitor

• Continuously monitors chemical

contamination events.

• On-Line - sends alerts in real-time.

• Key element of an Early Warning

System. CCB-TOX

Introduction: key benefits

Significantly reduce the threats associated with accidental and

intentional chemical contamination of water:

• Spills, accidents, dumping

• Equipment malfunction

• Natural disasters

• Sabotage & terror

• Acute & chronic exposure

• Illness & death

• Direct & indirect costs

• Liability suits

Introduction: key uses

• Drinking water monitoring: protecting public health by sensing changes

in water quality at reservoirs, intake, during & after treatment, throughout

the distribution network, protected zones, etc.

• Environmental monitoring: protecting the environment by sensing

changes in water quality along rivers, lakes & natural reservoirs, as well

as near potential effluent discharge areas.

• Water treatment monitoring: improving treatment processes

effectiveness by sensing water quality changes at intake, and during

treatment processes, enabling process modifications decisions & quality

assurance.

Introduction: potential users

• Water companies: raw water suppliers, drinking water utilities,

municipal water suppliers, water treatment plants.

• Authorities: environmental supervising & monitoring, river basin

monitoring, health supervising, municipal water systems.

• Secured facilities: industrial zones & parks, hospitals, governmental,

military.

• Industrial companies: water intake & discharge for bottlers, food and

other water related processing companies.

Introduction: Key Features (1)

On line, continuous monitoring, fully automated.

Highly sensitive, detects real toxicity.

Detection of a very broad range of contaminants.

Very fast detection & warning: less than 15 minutes.

Minimal false positive & negative response.

High overall system reliability.

Suspicious samples are captured by an automatic sampler for

further analysis.

Introduction: Key Features (2)

Remotely operated & controlled.

Requires minimal operational intervention.

Very easy to use - no special skills required.

Easy installation & maintenance.

No need for adjustments.

Easily integrated with other systems.

CCB-TOX is an automatic toxicity biomonitor based on

Bioluminescence.

Toxicity is the degree to which a substance is able to damage an

exposed organism.

A biomonitor (biological monitor) uses an organism (such as fish,

daphnia, ,mussels, algae, bacteria) that provides information about the

quality of the environment (i.e. water) around it.

Bioluminescence is a naturally occurring process by which a living

organism converts chemical energy into light.

Function – basics (1)

CCB-TOX is based on luminescent (light

emitting) natural bacteria.

Changes in the level of bacterial

luminescence (light) indicate potential

toxicity in the sampled water.

CCB-TOX is able to measure and analyze

changes in light levels, and as a result

transmit an alert in case of toxicity.

Function – basics (2)

Decreased luminescence

Luminescence Level reflects degree of toxicity

Function – luminous bacteria (1)

Pesticides, HerbicidesChlorinated hydro-CarbonsGasoline, petrol oilHeavy metalsDetergents

Clean sample

Contaminated sample

Membrane function, Protein & lipid synthesis ATP generation, Electron transport, Cell respiration

affecting

Freeze dried luminous bacteria are stable for long periods.

A million cells are introduced into a small water sample, resulting in

reduced effect of biological variability.

Chemical toxicants that effect cell’s metabolism lead to rapid decay of

luminescence.

High correlation exists between the luminescence test and bioassays that

apply higher organisms, such as fish.

Function – luminous bacteria (2)

“Bacteria-based biosensors are ideal for use as early warning screening tools for drinking

water security” (USEPA).

Function - Luminous bacteria (3)

Strain namePhotobacterium leiognathi

Vibrio fischeri

OriginSea water

Optimal temperature for light emission

18-35°C15-16°C

Toxicity test utilized in

PCB-TOX (ToxScreen3)

PCB-TOX-SPOT

CCB-TOX

MicrotoxTM

ToxControlTM

LumisToxTM

BioFixTM

Stability to changing environmental conditions

HighLow

Function - proprietary biotechnology

Reaction to a wide spectrum of chemical agents.

Sensitive to very low concentrations of toxicants.

Results are obtained in minutes.

Reliable and consistent results.

Preliminary discrimination between cationic heavy metals & organic

toxicants.

Long term stable storage of biosensor bacteria

Basic technology (ToxScreen) verified by the USEPA-ETV.

Examples of Detected Toxic Chemicals

Toxic

chemical1

Rat LD502

(mg/kg body weight)

CCB-TOX

IC50 (mg/L)

Potassium cyanide100.5

Arsenate(5)140.09

Cadmium(2)2250.2

Dieldrin461.2

Parathion143

Methiocarb600.08

Chlorpyriphos820.7

Note-1 - chemicals that might be used for intentional contamination according to US Army reportsNote-2 - lethal dose data taken from MSDS provided by manufacturers

1)Connected to inlet water and clean reference water. Continuously

(365/7/24) draws samples (at predetermined intervals) into two assay

chambers to detect: cationic heavy metals and organic toxicants.

2)The light sensor (PMT) records emitted luminescence and compares it to

the level obtained in the reference reading.

3)To minimize false alarms, once a toxicity state is noted (e.g. 50%

luminescence change), a repeat reading is taken and then a repeat

reference test. If the result repeats itself, a toxicity alarm is turned on,

4)The suspected sample is drained into the grab sample container for future

confirmation and analysis.

Function – monitoring process

Function – monitoring specs.

• Bioassay: freeze-dried luminous bacteria.

• Buffer system: dual - cationic heavy metals & toxic organic chemicals.

• Detector: photomultiplier tube.

• Contaminants detected: > 150 toxic chemicals tested so far.

• Sensitivity: for most chemicals, detection ranges from ppb to low ppm.

• Measuring interval: programmable.

• Measuring time: 5-60 minutes.

Function – unique features

High reliability & low maintenance:

Automatic positive control tests.

Double check before alert signaling.

Automatic cleaning & flushing.

Internal electronics self test mechanisms.

Replacement of all biofouling-prone elements – every 4 weeks.

Automatic remote signaling (alarms) of instrument malfunction.

Minimal hands on required for operation & maintenance.

Quick and easy monthly cartridge replacement//

Function – key elements

Key elements include:

Static unit includes various hardware components (cabinet,

electronics, mechanics).

Cartridge unit includes re-fill reagents (bacteria & buffers)

and replaceable tubing.

Management software includes various options – either

embedded or server-based components.

Function – system overview

Function – outside view

Function – connections

RS232 to GSM

RJ45 to LAN

Inlet water

Outlet water

Grab sample water two

Reference water

Grab sample water one Wash water

E s c E n t e r

AquaVerityCCB Continuous Contamination Biomonitor

Contamination Alert

All Clear

SystemMalfunction

Function – front panel

• Bright alert lights.

• Complete local set up and

control using easy press

buttons.

• Basic LCD - 4 lines x 20

characters, support

European fonts.

• Optional LCD with support

of Chinese / English – 2

lines x 8 characters,

(128x32 dots)

Operation – control panel

Operation buttons:"Enter" – choose command /

enter selected screen /

approve parameter.

"Esc" – back to previous screen /

silent buzzer.

"▲►" – rolling lines up / move to next character position on the right.

"▼" – rolling lines down /

character and digit rolling

cycle.

"Enter" + "Esc" – LED's + buzzer test.

E s c E n t e r

AquaVerityCCB Continuous Contamination Biomonitor

Contamination Alert

All Clear

SystemMalfunction

Operation – LCD display screens

Function – static unit

• Cabinet with display

• Microprocessor

• Light detection module (PMT)

• Heating/cooling units

• Connections to inlet/outlet tubes

• Gantry, Motors

• Grab samples containers

Function – hardware specs.

• Power Supply: 90-264 VAC (~690VA) up to 250W. DC output – 12V.

• Hardware: specially designed control card (microprocessor,

integrated keyboard and graphic LCD display) and motor card.

• Data storage: 256K Flash, 32K internal RAM, 1024K external RAM,

(downloadable - automatically / remote PC poll).

• Alarms: warning light +buzzer, LCD display, external communication

(user programmable).

• UPS – such as Advice PRPC750 or similar (required).

• Safety compliance: CE (EU) & UL (USA).

Preliminary – specification subject to change without notice

Function – physical specs

• Enclosure: IP55; IP65 on request

• Total weight: ~ 75 Kg without reagents

• Shipping weight: ~ 100 Kg.

• Size: 800x800x500 mm (H*W*D)

• Mounting: wall or rack mount

• Operating temperature: 5°C - 40°C

• Operating humidity: up to 95%


Function – hydraulic specs.

• Flow Rate: at least 60 Liter/hr. Pressure : 0.5-2 bar.

• Sample delivery: Inlet water pressure: 1.5 – 2 bar, Temperature: 5°C-

50°C, Volume: –at least 180 Liter/hr.

• Water connections: Standard 8x6 mm (N*), permanent female thread ¼

BSP + nipple. Various diameters supported.

• Waste: pressure free 16X14 mm; total accumulated volume (including

inlet, wash, and reagents) - 150Liter/month.

• Reagents heater/cooler: thermo-electric cooler.

• Maintenance interval: once every 4 weeks, ~15 minutes.

• External containers: grab sample, wash, reference water. Preliminary – specification subject to change without notice

Function – communication specs.

• Remote control: SMS (Short Message Service) over GSM (using

a 3rd party modem, i.e. EZ10 by Telit.).

• Data communication protocols: TCP/IP, Ethernet, GSM, SCADA

• Physical Communication port: Ethernet (RJ-45), RS-232

• Data reporting interval: every 24hrs

• Modbus protocol for connection to SCADA systems (optional

supported with CAS).


Function - cartridge

• Assay Buffers containers

• Freeze dried bacteria vials

• Syringes

• Assay chambers

• Disinfection Solution container

• Reference water container

• Bacterial suspension chamber

Function – Test cycle preparation

Toxicity/Reference test

• Dispense 200μl Metal/Organic buffer

• Dispense 800μl Reference or inlet water

• Mix by air bubble

• Dispense 15.5μl Hydrated bacteria solution


• Enter into the PMT box and read the luminescence every minute during 15 minutes

Positive control test

• Dispense 200μl Metal/Organic buffer

• Dispense 100μl Disinfection buffer (1:9 dilution)

• Dispense 700μl Reference water


• Dispense 15.5μl Hydrated bacteria solution


• Enter into the PMT box and read the luminescence every minute during 15 minutes

Org

ani

c Disin

fection

Metal

Referen

ce

Inlet

Function – cartridge reagents

Each cartridge contains a 4 weeks supply of the following reagents

(shipped at ambient temperature):

• Freeze dried bacteria vials (4).

• Pro-Metal Buffer

• Pro-Organic Buffer

• Disinfection (cleaning)/Positive control Solution

• Hydration/Storage Buffer

Two key options for management

software of the CCB:

• Basic control software.

• Advanced Control & Analysis

software – CAS.

Function - management software


Basic control software:

• Basic presentation of test results in graphical form. Remote set up

and control.

• Device-based, fully integrated, internal web server.

• IP-based proprietary communication protocol.

• One to one device management, configuration and reporting.

• SMS alerts.

• Supported languages: English, French, German, Italian, Spanish &

Chinese. On-line help file.

Function – basic software

Operation – Web server pages

Entrance Page – Password and authorities

Function – basic software

Function - Alarm state & Alert SMS messages

Alarm messageAlarm typeCasesToxicity alert – MetalicToxicityOrganic water contamination.Toxicity alert – OrganicToxicityMetalic water contamination.

Instrument malfunctionMalfunctionPositive control not working.Motor failure or Microcontroller's failure.

Instrument reduced functionalityMalfunction

Abnormal change (ascend or descend) in light level in reference water (relative level).Initial reference water light level is too low after inserting a new cartridge.

Temperature off limitsMalfunction

TEC (Thermo-Electric Cooler) failure. Temperature in bacteria chamber, assay chamber, storage buffer, inlet water and F.D. bacteria is off limits for a predefined period of time after power return.

Unauthorized cartridge insertedInformativeUn-authorized (damaged or used) cartridge unit insertion.

Cartridge needs replacementInformativeReagents volume reaching low level.Cartridge hasn't been replaced on time.

Power failure InformativePower supply failure.

Power failure recoveryInformativePower supply returned after failure.

Water flow problemInformativeNo inlet water flow.No reference water flow.

Unauthorized door open InformativeUnauthorized door open.Interlock cut off.

Function – advanced software


CAS –

Control &

Analysis

Software

Key features:

Easy remote monitoring and control of multiple CCB units.

Intuitive data report generation and trend analysis.

Alert management: e-mail, text-messages, cellular.

Additional features: advanced backup; log file analysis; open interfaces, API.

Alert management.

Smooth integration and communication with 3rd party devices & management

systems (such as SCADA/GIS).

Fully customized. Secure and reliable.

Function – CAS


Management, Communication and Security:

Application is managed either on external server (ASP / central database) or on

customer’s server on-site (application and database with remote installation &

support).

Connectivity - Either LAN (gateway & firewall), Cellular communication

(GSM/GPRS/CDMA/1X/UMTS/EVDO) or Satellite communication.

Access via iCex controller (scheduled polling of CCB units) using either Modbus /

TCP / COMP. No firewall settings are required. COMP communicated with

customers’ central system (SCADA)>

Secured data access (authorization levels, SSL) and integrity (central backup /

restore & disaster / recovery mechanism).Preliminary – specification subject to change without notice

Function – CAS

Function – CAS

Customization:

Fully customized: CCB parameters, user interface.

Different polling schedule per CCB data element / value.

Flexible format & validity checks per data element.

Change history per data element.

Customized user interface.

Secured access from anywhere: PC, Cell phone, PDA.

Simple and straightforward:

Physical mounting. Electricity, UPS and internet connection.

Plumbing: inert filter, external containers (3), pressure regulator.

Cartridge mounting. Assay vials insertion. Gantry positioning. (every

4 weeks – about 15 minutes work).

Parameters set up: general. Testing and communications.

Total first installation time: < 2 hours.

CCB - Installation & maintenance

Accessories & add-ons

• Alternative / renewable power supply (i.e. solar).

• Communication option: wire and wireless.

• Inlet water self cleaning filters.

• Inlet water pressure reducer.

• Water / wash containers.


CCB - Future Options

Detection of microbial outbursts in drinking water.

Tailor-made tests for designated groups of toxic chemicals

of interest.

Detection of bacterial re-growth potential / bio-stability of

drinking and process water.


Application

Key part of a comprehensive Early Warning Solution:

• Effective coverage of drinking water systems.

• Located at various stations throughout the water distribution system,

• Coupled with Portable Contamination Biomonitors.

• Pinpoint contamination boundaries & trace contamination sources.

• Sseamless integration with other monitors / sensors and customer

management systems (i.e. SCADA).

AquaVerity

xxx

AquaVerity Components

CCB - Continuous Contamination Biomonitor

PCB - Portable Contamination Biomonitor

CAS - Control & Analysis Software package

SIS - Solution Implementation Service package

Tiered Response Model

Increasing:

Certainty

Response

Cost

Observed Water Quality Change

)determined by broad-based

continuous screening(

Automated Sample Collection

Confirmation Bioassay

Chemical Analysis

If positive

Public health Regulatory or Remedial Action

If positive

PCB-TOX-SPOT

CCB-TOC

Applications

CheckLight’s Portable Contamination Biomonitors:

PCB-TOX-SPOTPCB-TOX PCB-AOC

How does the AquaVerity solution

compare to other solutions?

EWS Matrix (1)- Detection & Warning Capabilities

FactorMulti-ParameterOther Biomonitors

CheckLight’sAquaVerity

Method5-6 sensors5-20 live organismsOne million luminescent bacteria

Detection spectrum5-6 parametersWide range of contaminants, including unknown types.

Determines toxicityNoYesYes

Discrimination between organic & heavy metals Contaminants

NoNoYes

Detection sensitivity

Medium (depends on the parameter)

HighHigh

ContaminationBoundaries assessment

PartialNoYes, by using the portable detectors

EWS Matrix (2)- Implementation

FactorMulti-ParameterOther BiomonitorsCheckLight’sAquaVerity

Installation & maintenance

Complex due to variability of sensors used

Complex & requires on going human supervision

Very simple to install & maintain. Unattended operation.

Adjustment to changing water environment

ComplexComplexMinimal adjustment needed.

Effective coverage in large water networks

Limited to wide distribution. Depends on parameters mix & complexity

Limited distribution due to complexity & costs

Wide distribution possible due to simple installation, minimal training & maintenance.

On-Going usageMedium – requires skills & training

Complex - Requires skilled personnel & special training

Effort is minimal - reagents replacement once a month.

Overall reliabilityMedium. Depends on parameters used.

Low to Medium. Depends on biota used & environment. conditions

Very high. Includes built-in control mechanism.

Future EnhancementsunknownUnknownUpgrade re-fill kits with enhanced detection capabilities

EWS Matrix (3) - Cost Effectiveness

FactorMulti-ParameterOther BiomonitorsCheckLight’sAquaVerity

Initial capital investment

Low to very highDepending on chosen parameters

Medium to very highMedium

On going costsMedium due to complexity of sensors arrays and baseline build up

High due to the required human supervision

Low due to minimal intervention

Total cost of ownership

Medium to highHighLow to medium

Positioning

Sensitive to a broad

range of

contamination sources

Reliable

Cost effective

Easy to operate

Customer oriented

Unique Selling Points

Broad detection capabilities – range & sensitivity

Customer friendly – easy to install, operate & maintain

Reliable – minimum false positives & negatives

Very cost effective for broad EWS implementation

CCB vs ToxControl

FeatureCheckLightCCB-TOX

MicrolanToxControl

SensitivityVery highLow-medium

Discrimination between organic toxicants and cationic heavy metals

YESNO

RepeatabilityHighLow-medium

Needs adjustment to chlorinated water

NOYES

Measuring cycle5-15 minutes30 minutes

UPS installed & adjustedYESNO

Operational down-time per month~15 minutes16-20 hrs

Required skills for maintenanceLowMedium

Reagents re-fill frequencyOnce in 4 weeksOnce in 7 days

Set up timeShortLong

Confidential – for internal use only

IC50 examples (mg/L)

Tested chemicalCheckLightCCB-TOX

MicrolanToxControl

Arsenic (5)0.097.8

Cadmium (2)0.2420

Chromium (6)0.0715.3

DDT0.057

Mercury0.0140.07

Pentachlorophenol0.060.7


ToxControl – Key weaknesses (1)

No discrimination between heavy metals and organic contaminants.

Long measurement cycle – slow response time.

Hundreds of liters per week are required for reference water.

Management software is very limited. No 3rd party integration.

Complex and lengthy reagents preparations.

Customer requires a bio-reactor (which is +30% cost on top) with

special media.


ToxControl – Key weaknesses (2)

Down time is too long for on-line system.

Weekly maintenance. Manual cleaning.

Requires large space for installation.

Too expensive (total cost is +50% than other solutions).

Conclusion: not practical as a key element of a real Early Warning

solution where broad deployment including in remote locations is

required.


ccb-tox continuous contamination biomonitor benefits, functions & applications confidential...

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