cbse 2014 question paper for class 12 biotechnology - delhi

8/12/2019 CBSE 2014 Question Paper for Class 12 BioTechnology - Delhi

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Series : OSR/Ida+.Roll No.

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Candidates must write the Code onthe title page of the answer-book.

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Ewr fr m{ ii t* gq qFT-*r il zt Yw t rEqqr lrFr ET srrftrqiT rT66r+ + q6d, vvt u't Fqi6 wqrc ftrd tqqg{l;T-wrsiq-di+fuqtsfir+ai6.tIFI{[fqqr'rfltrq{{-{{orfl+aprFR-{ifto.tsq$fuqr qrnrn I 10.15 e.q t to.:o qs ffiF EEr fiq6 ssq-q-d q'i qan efu qs q-cfu a' qY{T arrr-gi.rr+r qr # rm roi frqti'r tPlease checkthat this question paper contains 7 printed pages'Code number given on the right hand side of the question papef should be written on thetitle page of the answer-book by the candidate'Please check that this question paper contains 28 questions'Please writedown the Serial Number of the question before attempting it'15 minutes time has been allotted to read this question paper. The question plperwill bedistributed at 10.15 a.m. From 10.15 a.m. to to.ro a.m', the students will read the

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question paper only and will not write any answer on the answer-book during this period'

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t atftfiiqeiq :70[Maximum Marks : 70

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General Instructions :O All questions are comPulsoryt.(ii) There is no overall choice. However, an internal choice has been provided in. one question of three marks and two questions of Jive marks. You have toattempt only one of tlte choices in such questions. Question paper contains four

sections - A, B, C and D.(iii) Question numbers I to 5 are very short answer questions, canying 1 mark each.(iv) Question numbers 6 to 15 are short answer questions, carrying 2 marks each.(v) euestion numbers 16 to 25 are also short answer questions, carrying 3 markseach.(vi) Question numbers 26 to 28 are long answer questions, cartying 5 marks each.

(vii) Use of calculators tr not permitted. However, you may use log tables,if necessarY.

1.

Ers_ 3TSECTION _ A

Tdr{qfuEffiFrw +qi qt}-qi} qtjf ri t qo+tq qr r's tfu{-sT {6iq qiit +qffi Y-*rqirit rIndicate which one of the two broad areas of genomics addresses thebiologicalfunctions of genes.qlm q{{i*h foKs{i ii q}qtdi * qqq f geq* fuqr qTi qrf,r 5q sTrqRHr t ?What isthe major criterion used in selecting nutrients for large scale culturing ?

"rh1frffif ffi]q" wr Etdr t r \ts sqr{q {li{q IWhat isa 'cell clone' ? Give an example.

sfui O{r-ir qrxb ti sffiqr EtrT Bit enqq{f,t otY{-{t t+-q 96FK -ffi lmqr qror t rWhy is oxygen important in fermentors and how is this provided ?

5. g6dqrflq td Evwaruutqrkfu+]ftrsr {iq?i tdq qEFKsrH H qri t rWith an example, explain how secondary cell cultures are derived.

4.

99tr

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tz. ffi \16 t{iFf,r E+qi it \'6 fefrrs q. + w-+T *1 qa-tn:t m.d { tsr uit t tr frqqtlr rTlqT* oNa mr rfr v6quuti;rn&ftrr f+qr wor t zIn identifying parents of a given child in a paternity suit, why is the undisputed mothers'DNA also typed / fingerPrinted ?

13. fulEFffir fr-{r'it it sNps +-Bqqtrr si qflti tE q'osqrfl"r {tqq tGive an example to demonstrate the use of SNPs in medical sciences'

**rs sn-o'*il mrqr qkTr t ? qoffirs fs-{T rcnnsq+rfr dat t tHow is callus tissue generated ? How is this tissue useful ?

ls. +i + qr {fr ilfunI it qtraqrttkfiss qfdTq{r ilTr }fr tfirg{ ql ffiq {rtg Hqr +{@*dt'

drkfir o.r w*+.pr m.rfr gqq) iqlur ftrslfiTq IGiven below is a table of the genome size andthaliana and Homo saPiens :

gene numbers of the weed Arabidopsis

Derive two observations with explanation of the table'4

14.

qfrGrEftm*{

15,70,00,0003,00,00,00,000

No. ofChromosomes

15,70,00,0003,00,00,00,000

99ll

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EIUE _HSECTION - C

16. a frfrtr u-sn +'ordq +{-*t{ t t.il +ctit +1 :-o {rfir d dqqr 6{fr t ? qlsr fi, qa,fiearqqfls+t{ t endq H{rxt +fr t sil{*tt-t gde r .rd fsqqri qrd*+ ld$il Edt d qri t zIndicate various types of bonds which contribute to 3-D structures in proteins. Alsomention which bonds are strong and which are weak. Why do proteins denature whenheated ?

t7 . .arl-oqs wr Etfr t sfu rDNA ry { t fus nmn gqftft dt t rWhat are plasmids and how are they useful in rDNA technology ?18. cML ttFii d ('ft;itrCfuiqT 6)ffiq' 6tdr t r gq Hirmq qt fuq ,FfiK q6qFT +1qrtr t r qq

n#*.+fr'qq d rro entq tt Ev warw ICML patients have the 'Philadelphia Chromosome'. How is this chromosomedetected ? Explain the technique with a diagram.

le. qqriftfr {-qd=ii *i qk Td,rftffi1*t qrq{r Edi T{nWi t r s+r+t #r ffiqq frftn} *'il-qfrkq ekH sTrq q+it t sd$ qKT sl-rrrcsrflfr t ss#fusq d qaeg rWhy is it important to measure growth kinetics of microbial cultures ? Name threedifferent methods and highlight the one you think is most effective.

20. ffi qfi Ehq +q sT TrqiGrd ente r+rgq I st fuq rFFK grT{n qrdr t *{ A Bfi sqq}rfrdttrDraw a labelled diagram of an artificial seed. How are these produced and why are theyuseful ?

lP.T.O.9n

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zt. qr"ft sjfrrspit sr tirq lrfiR risnur toqr qrcm t, 3t{ qe riqf{ tE gq-dr 3{lq{rFFf,I E]_fi t frs+1*" rorct ga'dfua mtEqr qnr t rHow are animal cells stored and how are they revived when required for culturing ?

22. ffi kE rrq sfr{ si ffi q6+. g rfl-6q 6i ffi}fr,T q-r} it wt-HI Eq q{q 3il-fr t entdq sqit qarqq rDiagrammatically indicate the important steps in cloning a given gene into a vector'

23. ffifrsrcni d {ffi +it *1 Hfi{qtn it tvr sTrt sT* qi qqs( {nqg r q-{tqK qri + flaq

+{a*ifrrflefi q,r [s{ rqnsq*rr i+qr qr s+-or t rqerEt

tefunq wr tn t r r*q qtq-trq Bilrqt sT qrci tt taffifrw mr f*-s rronsq+r tsqrqrar t rIndicate two problems which are encountered in expressing eukaryotic genes inbacteria. How can yeast cells be used to overcome these ?ORWhat is a metagenone ? How is metagenomics used to screen for novel microbialproducts ?

24. sruft u,1frrsr 1iqlh q6t rDNA SlBlffi t ffi* qri erd o: sqdrfr vt&{ q)Nfrrit *'qrqfufuqilTr sd t ffi qi +gqqt'r Sfrw it qqseq tName six useful protein pharmaceuticals derived from animal cell culture and rDNAtechnology and explain the use of any two'

zs. \r6dfr {ffi{ A* q-{Tfr qIfr t ? {T sT ott 1rowe'$ er1q-+'r qnl{g lHow are monoclonal antibodies produced ? Give one important application of the same'

99t1

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28. tqrrq orEd&fiqr +q-fi qerfi t qe q*,'f&q ffifr (-{rEq *laret+u rsr{o.r{ o.ri err"Trdr t ? tS qi efu-ffi * qrq ftfiqqcil {S Mqfu m.r Bqtrrr mrfr t rExplain how the folding of the enzyme chymotrypsin leads to its function as aproteolytic enzyme. Name two more enzymes which use the same mechanism.

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cbse 2014 question paper for class 12 biotechnology - delhi

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