castel_figure s1, related to figure 1
DESCRIPTION
Castel_Figure S1, related to Figure 1. RT-qPCR: Hey1. A. B. Relative mRNA levels (2^- DD Ct ). Sensitivity. GimmeMotifs. Transfac M00234. 1-Specificity. C. D. ChIP: Ab1-RBPJ. WT. Rbpj —/—. % ChIP /input. Krt9. Hey1. neg16. Rbms1. Rps6ka1. Apobec3. Atp6v0a4. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/1.jpg)
BA
C D
E
F
Castel_Figure S1, related to Figure 1
Rel
ativ
e m
RN
A le
vels
(2^-DD
Ct)
RT-qPCR: Hey1%
ChI
P/in
put
ChIP: Ab1-RBPJ
Sen
sitiv
ity
1-Specificity
GimmeMotifs
Transfac M00234
WT Rbpj—/—
RBPJ Phalloidin Hoechst
Hey
1
Genes associated to peaks
neg1
6
Rbm
s1
Atp
6v0a
4
Krt
9
Apo
bec3
Rps
6ka1
![Page 2: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/2.jpg)
D
C
Castel_Figure S2, related to Figure 2
Replicate experiment ChIP:Ab1-RBPJ
On OffNotch: On Off6 hours 24 hours
Con
stan
tIn
duci
ble
p300RBPJC
onst
ant
Indu
cibl
e
On Off
24 hours
Notch: On Off
RBPJ
Con
stan
tIn
duci
ble
RBPJ
Notch: On Off24 hours
On Off6 hours
![Page 3: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/3.jpg)
A
Castel_Figure S3
Validation with an independent RBPJ polyclonal antibody (Ab2-RBPJ)
On OffNotch: On Off
Ab1-RBPJ Ab2-RBPJ
Con
stan
tIn
duci
ble
Con
stan
tIn
duci
ble
Notch: On Off
Ab1-RBPJ
On Off
Ab2-RBPJB
![Page 4: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/4.jpg)
CREST motif in Ab2-RBPJ only sites
RBPJ (GM) RBPJ (M00234) REST CREB ETS
FE
Castel_Figure S3F
requ
ency
D
Sen
sitiv
ity
1-Specificity 1-Specificity
Ab2-RBPJ only sites Ab1-RBPJ sites
%C
hIP
/inpu
t
ChIP: Ab1-RBPJ ChIP: Ab2-RBPJ
ChIP on REST sites
%C
hIP
/inpu
t
ChIP: Ab2-RBPJ
Hey
1
Genes associated to peaks
neg1
6
Rbm
s1
Atp
6v0a
4
Krt
9
Apo
bec3
Rps
6ka1
peaks:
![Page 5: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/5.jpg)
A
C
RBPJ/NICD-GFP NICD-GFP only RBPJ only Constant RBPJ only Inducible
Castel_Figure S4, related to Figure 3
NICD-GFP GFP
HEYL
49kD
38kD
28kD
freq
uenc
y
freq
uenc
y
freq
uenc
y
freq
uenc
y
position (bp) position (bp) position (bp) position (bp)
B
D E
![Page 6: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/6.jpg)
F
Castel_Figure S4, related to Figure 3
![Page 7: Castel_Figure S1, related to Figure 1](https://reader036.vdocuments.site/reader036/viewer/2022062323/56815753550346895dc4f851/html5/thumbnails/7.jpg)
Castel_Figure S5, related to Figure 4
A
B
C