Cardiovascular System: The Blood

Dr. Michael P. Gillespie

Constituents Of BloodBlood is a connective tissue composed of a

liquid matrix called plasma that dissolves and suspends various cells and fragments.

Interstitial fluid is the fluid that bathes body cells.

Functions of BloodTransportation – oxygen, carbon dioxide,

nutrients, hormones, heat, & waste products.

Regulation – maintains homeostasis (ph, heat, osmotic pressure).

Protection – clotting, WBCs, & antibodies.

Physical Characteristics Of Blood

Blood is denser and more viscous than water.

The temperature is 38 degrees C (100.4 degrees F).

Slightly alkaline ph ranging from 7.35 to 7.45.

20% of the extracellular fluid (about 8% of total body mass).

The blood volume is 5 to 6 liters (1.5 gal) in an average adult male and 4 to 5 liters (1.2 gal) in an average adult female.

Withdrawing BloodBlood samples for laboratory testing are

obtained in various ways.

Venipuncture (the most common method).

A finger or heel stick is utilized for children and people who must monitor their blood daily (I.E. Diabetics).

An arterial stick is utilized when the level of O2 must be determined.

Components Of BloodWhole blood has two components:

Blood plasma, a watery liquid matrix that contains dissolved substances.

Formed elements (cells and cell fragments).

45% formed elements and 55% plasma.

Blood PlasmaA straw-colored liquid which is about 91.5%

water and 8.5% solutes.

Plasma proteins – maintain osmotic pressure.Albumins, globulins, and fibrinogen (synthesized by

hepatocytes).Gamma globulins (antibodies 0r immunoglobulins).

Electrolytes, nutrients, regulatory substances (i.E. Enzymes and hormones), gases, & waste products (urea, uric acid, creatinine, ammonia, and bilirubin).

Formed ElementsRBCs.

WBCs – perform many functions.

Formed ElementsPlatelets – cell fragments.

Formed ElementsHematocrit – the percentage of total

blood occupied by RBCs.Males have a higher hematocrit than females

because testosterone stimulates the production of erythropoeitin (EPO).

Menstruation leads to lower values for females during their reproductive years.

Anemia is a significant drop in the hematocrit.Polycythemia is an. Abnormally high percentage of RBCs.

Causes of PolycythemiaAbnormal increase in RBC production.

Tissue hypoxia.

Dehydration.

Blood doping or use of Epoetin alfa (Procrit or Epogen) by athletes.This increases the work load of the heart.The increased #s of RBCs raise the viscosity of the

blood, which increases the resistance to blood flow. This can cause high blood pressure and stroke.

Formation of Blood CellsHemopoiesis (hematopoiesis) – the

process by which the formed elements of blood develop.

Blood cells, macrophages, reticular cells, mast cells, and adipocytes arise from the red bone marrow.

Pluripotent stem cells in the bone marrow reproduce themselves, proliferate and differentiate into mature blood cells.

Two Types of Pluripotent Stem Cells

Myeloid stem cells.Give rise to red blood cells, platelets, monocytes,

neutrophils, eosinophils, and basophils.

Lymphoid stem cells.Give rise to lymphocytes.

Generations Of Cell Lines In The Development Of Blood

CellsPluripotent stem cells – mesenchymal cells

which have the capacity to develop into many different types of cells. They can reproduce themselves.

Progenitor cells – cannot reproduce themselves.

Precursor cells (blasts) – they develop into the actual formed elements of the blood.

Hormones That Regulate Blood Cell Development

Hemopoietic growth factors – regulates differentiation and growth of progenitor cells.

Erythropoietin (EPO) from the kidneys – increases the # of RBC precursors.

Thrombopoietin (TPO) from the liver – stimulates the formation of platelets.

Colony-stimulating factors (CSFs) or interleukins stimulate WBC formation.

Medical Uses of Hemopoietic Growth Factors

EPO is utilized in end stage kidney disease to increase RBC formation.

CSFs are utilized to stimulate WBC formation in cancer patients undergoing chemotherapy.

Thrombopoietin helps induce platelet formation in chemotherapy patients.

Red Blood CellsRed blood cells (RBCs) or erythrocytes:

Contain hemoglobin – oxygen-carrying protein which gives the cell its color.

Approximately 5 million RBCs are present per microliter of blood.

Approximately 2 million RBCs are created and destroyed per second.

RBC AnatomyBiconcave discs with a diameter of 7-8

micrometers.

The plasma membrane is flexible, which allows them to deform without rupturing as they squeeze through capillaries.

RBCs lack a nucleus and other organelles.

RBCs cannot reproduce or carry on extensive metabolic activities.

RBC PhysiologyWith no nucleus, RBCs have more space

available for oxygen transport.

RBCs lack mitochondria and generate ATP anaerobically; Consequently, they do not use up the oxygen they are transporting.

RBC PhysiologyThe biconcave disc has a greater surface area

allowing greater diffusion of gas molecules.

Hemoglobin binds to oxygen, carbon dioxide, and nitric oxide (NO).

NO causes vasodilation which enhances oxygen delivery to cells.

RBC Life CycleRed blood cells live only about 120 days.

Macrophages in the spleen and liver remove dead RBCs through phagocytosis.

Hemoglobin is broken down into its globin and heme portions.Globin is broken down into amino acids, which are

reused for proteins.Heme is converted into the yellow-orange pigment

bilirubin.

Reticulocyte CountThe rate of eryhtropoiesis is measured by a

reticulocyte count.

Low “retic” count – could indicate a shortage of erythropoietin due to a nutritional deficiency or leukemia.

High “retic” count – could indicate a good response to previous blood loss, iron therapy, or illegal use of Epoetin by an athlete.

White Blood CellsWhite blood cells (WBCs) or leukocytes have a

nucleus and do not contain hemoglobin.

Classified as either granular or agranular.

Types Of White Blood Cells

Eosinophil

Basophil

Neutrophil

Small lymphocyte

Monocyte

Numbers Of WBCsRBCs outnumber WBCs by about 700:1.

There are approximately 5000 – 10,000 cells per microliter of blood.

Leukocytosis – an increase in the number of WBCs due to stresses such as microbes, strenuous exercise, anesthesia, or surgery.

Leukopenia – a decrease in the number of WBCs due to radiation, shock, or chemotherapy.

Functions Of WBCsThe WBCs combat pathogens by phagocytosis

and other immune responses.

WBCs leave the bloodstream by emigration.

Functions Of WBCsNeutrophils and macrophages are active in

phagocytosis.

Phagocytes are attracted to inflamed tissues through a process called chemotaxis.

Phagocytes release the enzyme lysozyme, which destroys certain bacteria.

Functions Of WBCsEosinophil – release histamines. Respond to

allergic responses and parasitic infection.

Basophil – liberate heparin, histamine, and serotonin. They intensify the inflammatory reaction and are involved in hypersensitivity (allergic) reactions.

Functions Of WBCsNeutrophil – active in phagocytosis and ingest

bacteria and dead matter. They respond to bacteria and fungi.

Lymphocyte – “soldiers” of the immune system.

Monocyte – turn into macrophages and clean up cellular debris after an infection.

Differential WBC CountUtilized to detect infection, inflammation,

poisoning, blood disorders, effects of chemotherapy, allergic reactions, and parasitic infections.

Significance Of High & Low WBC Counts

WBC Type High Count Low Count

Neutrophils Bacterial infection, burns, stress, inflammation

Radiation, drug toxicity, B12 deficiency, SLE

Lymphocytes Viral infection, leukemia

Prolonged illness, immunosuppression, cortisol tx.

Significance Of High & Low WBC Counts

Monocytes Viral or fungal infection, chronic diseases

Bone marrow suppression, cortisol tx.

Eosinophils Allergic rx., parasites, autoimmune dis.

Drug toxicity, stress

Basophils Allergic rx., cancers, hypothyroidism

Pregnancy, ovulation, stress, hyperthyroidism

PlateletsThe hormone thrombopoietin influences the

production of platelets (thrombocytes).

Platelets help stop blood loss by forming a platelet plug.

Complete Blood Count (CBC)

A test that screens for anemia and various infections.

Counts of RBCs, WBCs, platelets, hematocrit, and a differential white blood cell count are included.

Blood ClottingSerum is a straw colored liquid and the gel is

called a clot.

The process of gel formation is called clotting or coagulation.

Clotting factors are involved in the coagulation cascade.

Blood ClottingNormal clotting requires vitamin K, which is

produced by bacteria in the intestines.

Dissolution of a clot is called fibrinolysis.

Anticoagulants (heparin & Warfaring a.K.A. Coumadin) are utilized for patients at risk of forming a blood clot.

Intravascular ClottingThrombosis – clotting in an unbroken

blood vessel.

Thrombus – the clot itself.

Embolus – a blood clot, bubble of air, fat from broken bones, or a piece of debris transported by the bloodstream.

Pulmonary embolism – when an embolus lodges in the lungs.

ABO Blood GroupType A blood - RBCs display only antigen

A.

Type B blood – RBCs display only antigen B.

Type AB blood – RBCs display both antigens A & B.

Type O blood – RBCs display neither antigen A or B.

ABO Blood GroupThe blood plasma contains isoantibodies or

agglutinins that react with A or B antigens if mixed.

You do not have the antibodies that react with the antigens of your own RBC type, but you most likely have the antibodies for antigens your own blood lacks.

Rh Blood GroupPeople with Rh antigens in their blood are Rh

positive and people without the Rh antigen are Rh negative.

Normally, plasma does not contain anti-Rh antibodies.

Rh Blood GroupIf an Rh- person receives Rh+ blood transfusion,

he or she will start to make antibodies that will remain in the blood.

The next time he or she receives Rh+ blood, the antibodies will cause hemolysis of the donated blood.

Hemolytic Disease Of The Newborn

ABO Blood Group Interactions

Characteristics A B AB O

Antibody in plasma

Anti-B Anti-A Neither Both

Antigen on RBCs

A B A & B Neither

Donors A, O B, O A, B, AB, O

O

Hemolysis B, AB A, AB None A, B, AB