campbell6e lecture ch2
TRANSCRIPT
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Mary K. CampbellShawn O. Farrellhttp://academic.cengage.com/chemistry/campbell
Chapter TwoWater: The Solvent for Biochemical Reactions
Paul D. Adams University of Arkansas
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What makes water polar
What is a polar bond:
• Electrons are unequally shared,more negative charge found closer to one atom.
• Due to difference in electronegativity of atoms involved in bond.
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Electronegativity
• Electronegativity:Electronegativity: a measure of the force of an atom’s attraction for electrons it shares in a chemical bond with another atom• Oxygen and Nitrogen, more electronegative than
carbon and hydrogen• Fluorine is most electronegative (4)
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Polar Bonds & Molecules
• Molecules such as CO2 have polar bonds but, given their geometry, are nonpolar molecules; that is, they have a zero dipole moments
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Solvent Properties of H2O
• Ionic compounds (e.g.,KCl) and low-molecular- weight polar covalent compounds (e.g., C2H5OH and CH3COCH3) tend to dissolve in water
• The underlying principle is electrostatic attraction of unlike charges; the positive dipole of water for the negative dipole of another molecule, etc.• ion-dipole interaction: e.g., KCl dissolved in H2O
• dipole-dipole interactions: e.g., ethanol or acetone dissolved in H2O
• dipole induced-dipole interactions: weak and generally do not lead to solubility in water
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Hydration Shells Surrounding Ions in Water
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• Ion-dipole and dipole-dipole interactions help ionic and polar compounds dissolve in water
Ion-dipole and Dipole-dipole Interactions
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Solvent Properties of H2O
• Hydrophilic: water-loving• tend to dissolve in water
• Hydrophobic: water-fearing• tend not to dissolve in water
• Amphipathic: has characteristics of both properties• molecules that contain one or more hydrophobic and
one or more hydrophilic regions, e.g., sodium palmitate
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Amphipathic molecules
• both polar and nonpolar character• Interaction between nonpolar molecules is very weak
– called van der Waals interactions
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Micelle formation by amphipathic molecules
• Micelle:Micelle: a spherical arrangement of organic molecules in water solution clustered so that• their hydrophobic parts are buried inside the sphere • their hydrophilic parts are on the surface of the sphere
and in contact with the water environment• formation depends on the attraction between
temporary induced dipoles
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Examples of Hydrophobic and Hydrophilic Substances
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Hydrogen Bonds
• Hydrogen bond:Hydrogen bond: the attractive interaction between dipoles when:• positive end of one dipole is a hydrogen atom bonded
to an atom of high electronegativity, most commonly O or N, and
• the negative end of the other dipole is an atom with a lone pair of electrons, most commonly O or N
• Hydrogen bond is non-covalent
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Interesting and Unique Properties of Water
• Each water molecule can be involved in 4 hydrogen bonds: 2 as donor, and 2 as acceptor
• Due to the tetrahedral arrangement of the water molecule (Refer to Figure 2.1).
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Hydrogen Bonding
• Even though hydrogen bonds are weaker than covalent bonds, they have a significant effect on the physical properties of hydrogen-bonded compounds
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Other Biologically Important Hydrogen bonds
• Hydrogen bonding is important in stabilization of 3-D structures of biological molecules such as: DNA, RNA, proteins.
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Acids, Bases and pH
• Acid:Acid: a molecule that behaves as a proton donor
• Strong base:Strong base: a molecule that behaves as a proton acceptor
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• One can derive a numerical value for the strength of an acid (amount of hydrogen ion released when a given amount of acid is dissolved in water).
• Describe by Ka:
• Written correctly,
Acid Strength
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Ionization of H2O and pH
• Lets quantitatively examine the dissociation of water:
• Molar concentration of water (55M)
• Kw is called the ion product constant for water.
• Must define a quantity to express hydrogen ion concentrations…pH
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Henderson-Hasselbalch
• Equation to connect Ka to pH of solution containing both acid and base.
• We can calculate the ratio of weak acid, HA, to its conjugate base, A-, in the following way
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Henderson-Hasselbalch (Cont’d)
• Henderson-Hasselbalch equation
• From this equation, we see that• when the concentrations of weak acid and its
conjugate base are equal, the pH of the solution equals the pKa of the weak acid
• when pH < pKa, the weak acid predominates• when pH > pKa, the conjugate base predominates
[Weak acid]
[Conjugate base]log=pH pKa +
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Titration Curves
• Titration:Titration: an experiment in which measured amounts of acid (or base) are added to measured amounts of base (or acid)
• Equivalence point:Equivalence point: the point in an acid-base titration at which enough acid has been added to exactly neutralize the base (or vice versa)
• a monoprotic acid releases one H+ per mole• a diprotic acid releases two H+ per mole• a triprotic acid releases three H+ per mole
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Buffers
• buffer:buffer: a solution whose pH resists change upon addition of either more acid or more base• consists of a weak acid and its conjugate base
• Examples of acid-base buffers are solutions containing• CH3COOH and CH3COONa
• H2CO3 and NaHCO3
• NaH2PO4 and Na2HPO4
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Buffer Range
• A buffer is effective in a range of about +/- 1 pH unit of the pKa of the weak acid
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Buffer Capacity
• Buffer capacity is related to the concentrations of the weak acid and its conjugate base• the greater the concentration of the weak acid and its
conjugate base, the greater the buffer capacity
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Naturally Occurring Buffers
• H2PO4-/HPO4
2- is the principal buffer in cells• H2CO3/HCO3
- is an important (but not the only) buffer in blood
• hyperventilation can result in increased blood pH• hypoventilation can result in decreased blood pH(Biochemical Connections p. 60)
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Selecting a Buffer
• The following criteria are typical• suitable pKa
• no interference with the reaction or detection of the assay
• suitable ionic strength • suitable solubility• its non-biological nature
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Laboratory Buffers