Cambridge Light Microscopy Spring Course 2017

Organisers:Stefanie Reichelt

Nick BarryKevin O’Holleran

Alex SossickHeather Zecchini

Speakers:Brad AmosGopi Shah

Kevin O’ HolleranStefanie Reichelt

Dario BressanGeorge Sirinakis Edward Allgeyer

Aleks Ponjavic

(MRC LMB)(CRUK CI)(CAIC)(CRUK CI)(CRUK CI)(Gurdon)(Gurdon)(Gurdon)

Focus on light sheet, super-resolution and live cell imaging

Location: CRUK Cambridge Institute (CRUK CI)MRC LMBCambridge Gurdon Institute Cambridge Advanced Imaging Centre (CAIC)

25- 27 April, 2017

(CRUK CI)(MRC LMB)(CAIC)(Gurdon)(CRUK CI)

The course is aimed at experienced microscopy users and developers, who wish to connect with imaging experts and core facilities in and around Cambridge. We are looking to provide expertise and knowledge in new imaging techniques and an overview of what is avail-able, as well as identifying multidisciplinary opportunities to facilitate scienti�c collaborations. The course will also provide a link to microscopy companies for the discussion of emerging techniques and applications for new scienti�c questions.

Cambridge  Light  Microscopy  Spring  Course  

Tuesday  25th-­‐  Thursday  27th  April  2017  

www.lightmicroscopy.cruk.cam.ac.uk  contact:  microscopy  (at)  cruk.cam.ac.uk  

 

The  course  is  aimed  at  experienced  microscopy  users  and  developers,  who  wish  to  connect  with  imaging  experts  and  core  facilities  in  and  around  Cambridge.  We  are  looking  to  provide  expertise  and  knowledge  in  new  imaging  techniques  and  an  overview  of  what  is  available,  as  well  as  identifying  multidisciplinary  opportunities  to  facilitate  scientific  collaborations.  The  course  will  also  provide  a  link  to  microscopy  companies  for  the  discussion  of  emerging  techniques  and  applications  for  new  scientific  questions.  

The  course  will  consist  of  practical  rotations  and  seminars  based  in  several  locations  in  Cambridge.    

Places  are  limited  to  16  for  the  practical  sessions.  Seminars  are  open  to  all.    

Course  fees  are  £200  for  the  three  days  including  lunches  and  the  course  dinner  on  27th  April  2017.    

To  register  your  interest  in  the  course,  please  submit  the  short  Questionnaire  available  from  the  Website  by  email  (subject:  spring  course).    

Deadline  for  application:  17th  April.  Applicants  will  be  notified  by  20th  April.  

   

   

   Programme    DAY  1:  Whole  Organism  Imaging  09:00  Registration  &  Coffee  at  CRUK  CI  9:15  –  10:00:  Seminar  (CRUK  CI  Lecture  theatre)  Dr  Gopi  Shah:  Light  Sheet  Microscopy  Practical:  1. SPIM:  CRUK  CI  2. Zeiss  Z1;  CRUK  CI  3. Light  Sheet:  CAIC  4. Light  Sheet:  CAIC  12:30  –  13:30  Lunch  (CRUK  CI)  &  transfer  Practical:    1. Light  Sheet:  CAIC  2. Light  Sheet:  CAIC  3. SPIM:  CRUK  CI  4. Zeiss  Z1:  CRUK  CI  16:00-­‐17:00:  Seminar  (CRUK  CI  Lecture  theatre)  Dr  Kevin  O’Holleran:  Light  sheet  and  multi-­‐photon  imaging  at  CAIC    DAY  2:  Cellular  Imaging  9:15  –  10:00  Seminar  (CRUK  CI  Lecture  theatre)  Dr  Stefanie  Reichelt:  New  Approaches  in  Imaging  Cancer  Biology  Practical:  5. Zeiss  Intelligent  imaging:  CRUK  CI  6. Leica  Matrix  scanner:  CRUK  CI  7. Nikon  Elements  acquisition:  MRC  LMB  8. Nikon  Elements  analysis:  MRC  LMB  12:00  –  13:00  lunch  (CRUK  CI)  &  transfer      

   Practical:    1. Nikon  Elements  acquisition:  MRC  LMB  2. Nikon  Elements  analysis:  MRC  LMB  3. Zeiss  Intelligent  imaging:  CRUK  CI  4. Leica  Matrix  scanner:  CRUK  CI  16:00-­‐17:00:  Seminar  (CRUK  CI  Lecture  theatre)  Dr  Dario  Bressan:  How  to  make  cells  glow    DAY  3:  Super  Resolution  imaging  9:15  –  10:00:  George  Sirinakis  and  Edward  Allgeyer:  Super  Resolution  Imaging  in  Thick  Samples  (Gurdon  Institute)  Practical:  1. STED:  CRUK  CI  2. STED:  MRC  LMB  3. PALM/STORM:  Gurdon  Institute  4. SIM:  Gurdon  Institute  12:30  –  13:30  lunch  (MRC  LMB)  &  transfer  Practical:    1. PALM/STORM:  Gurdon  Institute  2. SIM:  Gurdon  Institute  3. STED:  CRUK  CI  4. STED:  MRC  LMB  16:00-­‐17:00:  Aleks  Ponjavic:  Title  TBC  (Gurdon  Institute)    17:00  Close    18:00:  Drinks  at  Wolfson  College  18:30:  Talk  by  Dr  Brad  Amos:  my  life  in  imaging  19:00:  Dinner  at  Wolfson  College