buttons in spm5 carolyn mcgettigan & alice grogan methods for dummies 5 th april 2006
TRANSCRIPT
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Buttons in SPM5
Carolyn McGettigan & Alice Grogan
Methods for Dummies
5th April 2006
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Introducing the SPM5 User Interface
Help in SPM5Spatial Pre-processing in fMRI1st and 2nd Level Analysis
Overview
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SPM5 User Interface
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SPM5 User Interface
Preprocessing
Analysis
Inference
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SPM5 User Interface
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Current List of Jobs
SPM5 User Interface
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Current List of Jobs
Options available for currently
highlighted object
Current value of /information about
highlightedobject
Save/Loadas .mat files or
XML (“load-xml”,“savexml”)
Info about the meaning of current item
SPM5 User Interface
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SPM5 Help
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Sample Data Set from SPM5 manual “Mother of All Experiments” (MoAE) Auditory fMRI data
Single-subject Whole-brain BOLD/EPI images acquired on 2T Siemens
MAGNETOM Vision system Each acquisition
64 contiguous slices (64x64x64 3mm x 3mm x 3mm voxels) 6.05s acquisition TR=7sec
96 acquisitions in total 16 blocks of 6 So each block 42 sec
Functional data starts at acquisition 4 Advisable to discard initial few scans due to T1 effects
Structural image also acquired
Spatial Pre-Processing
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1. Realignment
2. Coregistration
3. Segmentation
4. Normalize
5. Smoothing
Spatial Pre-processing
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Data are available from http://www.fil.ion.ucl.ac.uk/spm/data/
Create a new directory for data Create a subdirectory “jobs”
Open MATLAB Get into the correct working
directory Type “SPM fmri”
If you’re using SPM for the first time, make sure you “Set Path”, under File in MATLAB. Enter the path to your SPM folder and select the “Add with Subfolders” option
From start to finish…
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Click on “Realign” from drop-down menu
Realignment
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Select “New Realign:Estimate and Reslice”
Realignment
Open “Realign:Estimate and Reslice” option
Highlight Data and select “New Sesson”
Highlight “Session”
Select “Specify Files”
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Realignment
Choose all of the functional images in the directory i.e. images beginning
‘fM000*.img’
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Realignment Save job file as e.g.
“realign.mat” in your jobs directory
Press “RUN”
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Realignment
etc
Mean image for use in coregistration
Header files modified with orientation info
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Coregistration
Click on “Coregister”
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Coregistration Click on “New Coreg:Estimate”
Double-click on “Coreg:Estimate”
Highlight “Reference Image” select mean fMRI scan meanfM00223_004.img from realignment
Highlight “Source Image” select structural image sM00223_002.img
SAVE as ‘coreg.mat’
Press “RUN”
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Effects: SPM implements a
coregistration between structural and functional data that maximises mutual information
SPM changes header of source file i.e. sM00223_002.hdr
Coregistration
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Useful to check registration of ref and source images at this point
Click “Check Reg” button Select your source
and ref images as before
Navigate images and inspect anatomical correspondence
Coregistration
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Segmentation
Click on “Segment”
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Segmentation Highlight Data field
“Specify Files” select the subject’s registered structural image sM00223_002.img
Optional extras:“Custom” menu
For extra speed, though not optimal, you can reduce the number of Gaussians per class e.g. to [1 1 1 4]. Click on “Specify Text” to modify the values.
SAVE as segment.mat RUN
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Effects: SPM creates grey and white
matter images and a bias-field corrected structural image
View with Check Reg Grey matter image is
c1sM00223_002.img White matter image is
c2sM00223_002.img Check reg against original structural
sM00223_002.img
Segmentation
SPM also writes spatial normalisation and inverse spatial normalisation parameters to files in structural directory:
sM00223_002_seg_sn.mat sM00223_002_seg_inv_sn.mat
THESE CAN BE USED TO NORMALISE FUNCTIONAL DATA
Grey matterimage
Originalstructural image
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Click on “Normalize”
Normalize
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Select “Normalise:Write” Allows previously determined
warps to be applied to a series of images
Normalize
Highlight “Data”
Select new “Subject” Open “Subject” and highlight “Parameter File” Select sM00223_002_seg_sn.mat from Segmentation step
Highlight “Images to Write” “Specify Files” Use filter to select all realigned functional images Type ^r.* in SPM file selector and click “Filt” Right-click “Select all” Done
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Open “Writing Options” Click “Voxel sizes”, then
“Specifiy Values” Change values to [3 3 3] This writes images at a
resolution closer to that at which they were acquired
Normalize
SAVE as “normalise.mat
RUN
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Effects: SPM writes
spatially normalised files to the functional data directory
Normalised files have the prefix “w”
Normalize
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Smooth
Click on “Smooth”
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Open “Smooth”
Smooth
Select “Images to Smooth” select the spatially normalised files “wrfM00*.img”
Highlight “FWHM” “Specify Values” Change [8 8 8] to [6 6 6] Data will be smoothed by 6mm in each direction
SAVE as smooth.mat
RUN
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Effects See right
Normalised functional image above wrfM00223_004.img
Smoothed image below swrf00223_004.img
Note: SPM5 Manual says
“smoothing step is unnecessary if you are only interested in Bayesian analysis of your functional data”
Smooth
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fM00223_004.img Realign rfM00223_004.img Coregister Segment Normalise wrfM00223_004.img Smooth swrfM00223_004.img
Overview
Use SPM Help and the ? Button
See SPM5 Manual for everythingI’ve included today
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